R Haselberg

Summary

Affiliation: Utrecht University
Country: The Netherlands

Publications

  1. pmc Capillary electrophoresis-mass spectrometry using noncovalently coated capillaries for the analysis of biopharmaceuticals
    R Haselberg
    Department of Biomedical Analysis, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands
    Anal Bioanal Chem 400:295-303. 2011
  2. doi request reprint Low-flow sheathless capillary electrophoresis-mass spectrometry for sensitive glycoform profiling of intact pharmaceutical proteins
    Rob Haselberg
    BioMolecular Analysis, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands
    Anal Chem 85:2289-96. 2013
  3. doi request reprint CE-MS for the analysis of intact proteins 2010-2012
    Rob Haselberg
    BioMolecular Analysis, Utrecht University, CG Utrecht, The Netherlands
    Electrophoresis 34:99-112. 2013
  4. doi request reprint Characterization of drug-lysozyme conjugates by sheathless capillary electrophoresis-time-of-flight mass spectrometry
    R Haselberg
    BioMolecular Analysis, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, The Netherlands
    Anal Chim Acta 698:77-83. 2011
  5. doi request reprint Capillary electrophoresis-mass spectrometry for the analysis of intact proteins 2007-2010
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, TB Utrecht, The Netherlands
    Electrophoresis 32:66-82. 2011
  6. doi request reprint Performance of a sheathless porous tip sprayer for capillary electrophoresis-electrospray ionization-mass spectrometry of intact proteins
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Chromatogr A 1217:7605-11. 2010
  7. doi request reprint Capillary electrophoresis-mass spectrometry of intact basic proteins using Polybrene-dextran sulfate-Polybrene-coated capillaries: system optimization and performance
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    Anal Chim Acta 678:128-34. 2010
  8. doi request reprint Effectiveness of charged noncovalent polymer coatings against protein adsorption to silica surfaces studied by evanescent-wave cavity ring-down spectroscopy and capillary electrophoresis
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    Anal Chem 81:10172-8. 2009
  9. doi request reprint Capillary electrophoresis of intact basic proteins using noncovalently triple-layer coated capillaries
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, Utrecht, The Netherlands
    J Sep Sci 32:2408-15. 2009
  10. ncbi request reprint Capillary electrophoresis-mass spectrometry for the analysis of intact proteins
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, 3508 TB Utrecht, The Netherlands
    J Chromatogr A 1159:81-109. 2007

Collaborators

Detail Information

Publications11

  1. pmc Capillary electrophoresis-mass spectrometry using noncovalently coated capillaries for the analysis of biopharmaceuticals
    R Haselberg
    Department of Biomedical Analysis, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands
    Anal Bioanal Chem 400:295-303. 2011
    ..It is concluded that the separation power provided by combined capillary electrophoresis and TOF-MS allows discrimination of highly related protein species...
  2. doi request reprint Low-flow sheathless capillary electrophoresis-mass spectrometry for sensitive glycoform profiling of intact pharmaceutical proteins
    Rob Haselberg
    BioMolecular Analysis, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands
    Anal Chem 85:2289-96. 2013
    ..35 to 950 nM. These profiles may be very useful for quality control of biopharmaceuticals and their biosimilars...
  3. doi request reprint CE-MS for the analysis of intact proteins 2010-2012
    Rob Haselberg
    BioMolecular Analysis, Utrecht University, CG Utrecht, The Netherlands
    Electrophoresis 34:99-112. 2013
    ..Finally, some general conclusions and future perspectives are given...
  4. doi request reprint Characterization of drug-lysozyme conjugates by sheathless capillary electrophoresis-time-of-flight mass spectrometry
    R Haselberg
    BioMolecular Analysis, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, The Netherlands
    Anal Chim Acta 698:77-83. 2011
    ..Based on relative peak areas (assuming an equimolar response for each component) a quantitative conjugate profile could be derived for every preparation leading to drug loading values of 0.4-0.6 mol drug per mole protein...
  5. doi request reprint Capillary electrophoresis-mass spectrometry for the analysis of intact proteins 2007-2010
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, TB Utrecht, The Netherlands
    Electrophoresis 32:66-82. 2011
    ..It is concluded that CE-MS is a valuable technique with high potential for intact protein analysis, providing useful information on protein identity and purity, including modifications and degradation products...
  6. doi request reprint Performance of a sheathless porous tip sprayer for capillary electrophoresis-electrospray ionization-mass spectrometry of intact proteins
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Chromatogr A 1217:7605-11. 2010
    ..Clearly, the sheathless porous tip sprayer provides high sensitivity CE-MS of intact proteins...
  7. doi request reprint Capillary electrophoresis-mass spectrometry of intact basic proteins using Polybrene-dextran sulfate-Polybrene-coated capillaries: system optimization and performance
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    Anal Chim Acta 678:128-34. 2010
    ..With the CE-MS system, determination coefficients (R(2)) for protein concentration and peak area were higher than 0.996, whereas detection limits were between 11 and 19 nM...
  8. doi request reprint Effectiveness of charged noncovalent polymer coatings against protein adsorption to silica surfaces studied by evanescent-wave cavity ring-down spectroscopy and capillary electrophoresis
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    Anal Chem 81:10172-8. 2009
    ..It is concluded that the combination of results from EW-CRDS and CE provides highly useful information on the adsorptive characteristics of bare and coated silica surfaces toward basic proteins...
  9. doi request reprint Capillary electrophoresis of intact basic proteins using noncovalently triple-layer coated capillaries
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, Utrecht, The Netherlands
    J Sep Sci 32:2408-15. 2009
    ..Finally, the feasibility of using the PB-DS-PB coated capillaries for CE with mass spectrometric detection was shown by the characterization of the impure llama antibody sample...
  10. ncbi request reprint Capillary electrophoresis-mass spectrometry for the analysis of intact proteins
    Rob Haselberg
    Department of Biomedical Analysis, Utrecht University, 3508 TB Utrecht, The Netherlands
    J Chromatogr A 1159:81-109. 2007
    ..It is concluded that CE-MS represents a powerful tool for the analysis of intact proteins yielding unique separations and information...
  11. pmc Quality of original and biosimilar epoetin products
    Vera Brinks
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, Sorbonnelaan 14 16, 3584 CA, Utrecht, The Netherlands
    Pharm Res 28:386-93. 2011
    ..To compare the quality of therapeutic erythropoietin (EPO) products, including two biosimilars, with respect to content, aggregation, isoform profile and potency...