Manfred Wuhrer

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. ncbi request reprint IPSE/alpha-1, a major secretory glycoprotein antigen from schistosome eggs, expresses the Lewis X motif on core-difucosylated N-glycans
    Manfred Wuhrer
    Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands
    FEBS J 273:2276-92. 2006
  2. ncbi request reprint Negative-mode MALDI-TOF/TOF-MS of oligosaccharides labeled with 2-aminobenzamide
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Chem 77:6954-9. 2005
  3. pmc Pro-inflammatory pattern of IgG1 Fc glycosylation in multiple sclerosis cerebrospinal fluid
    Manfred Wuhrer
    Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
    J Neuroinflammation 12:235. 2015
  4. pmc Comparison of the Fc glycosylation of fetal and maternal immunoglobulin G
    Helga K Einarsdóttir
    Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Glycoconj J 30:147-57. 2013
  5. pmc Glycomics using mass spectrometry
    Manfred Wuhrer
    Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Albinusdreef 2, 2333ZA, Leiden, The Netherlands
    Glycoconj J 30:11-22. 2013
  6. pmc The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG
    Sarah Lebeer
    Centre of Microbial and Plant Genetics, K, U, Leuven, Kasteelpark Arenberg 20, Box 2460, B 3001 Leuven, Belgium
    Microb Cell Fact 11:15. 2012
  7. pmc Mannose-binding lectin does not explain the course and outcome of pregnancy in rheumatoid arthritis
    Fleur E van de Geijn
    Department of Rheumatology, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 50, NL 3015 GE, Rotterdam, The Netherlands
    Arthritis Res Ther 13:R10. 2011
  8. pmc Immunoglobulin G galactosylation and sialylation are associated with pregnancy-induced improvement of rheumatoid arthritis and the postpartum flare: results from a large prospective cohort study
    Fleur E van de Geijn
    Department of Rheumatology, Erasmus MC University Medical Center Rotterdam, Dr, Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Arthritis Res Ther 11:R193. 2009
  9. pmc Glycan labeling strategies and their use in identification and quantification
    L R Ruhaak
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300RC Leiden, The Netherlands
    Anal Bioanal Chem 397:3457-81. 2010
  10. ncbi request reprint Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands
    FEBS J 273:347-61. 2006

Collaborators

Detail Information

Publications96

  1. ncbi request reprint IPSE/alpha-1, a major secretory glycoprotein antigen from schistosome eggs, expresses the Lewis X motif on core-difucosylated N-glycans
    Manfred Wuhrer
    Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands
    FEBS J 273:2276-92. 2006
    ..This is the first report both on the expression of Lewis X on a specific schistosome egg protein and on a protein-specific glycosylation analysis of schistosome eggs...
  2. ncbi request reprint Negative-mode MALDI-TOF/TOF-MS of oligosaccharides labeled with 2-aminobenzamide
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Chem 77:6954-9. 2005
    ....
  3. pmc Pro-inflammatory pattern of IgG1 Fc glycosylation in multiple sclerosis cerebrospinal fluid
    Manfred Wuhrer
    Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
    J Neuroinflammation 12:235. 2015
    ..Intrathecal production of IgG, especially IgG1, is a hallmark of multiple sclerosis (MS), but nothing is known about IgG Fc glycosylation in MS and in cerebrospinal fluid (CSF) in general...
  4. pmc Comparison of the Fc glycosylation of fetal and maternal immunoglobulin G
    Helga K Einarsdóttir
    Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Glycoconj J 30:147-57. 2013
    ..Average levels of galactosylation, sialylation, bisecting GlcNAc and fucosylation were very similar for the fetal and maternal IgGs. Our data suggest that the placental IgG transport is not Fc glycosylation selective...
  5. pmc Glycomics using mass spectrometry
    Manfred Wuhrer
    Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Albinusdreef 2, 2333ZA, Leiden, The Netherlands
    Glycoconj J 30:11-22. 2013
    ..The given examples are demonstrating the application of mass spectrometry to study glycosylation changes associated with congenital disorders of glycosylation, lysosomal storage diseases, autoimmune diseases and cancer...
  6. pmc The major secreted protein Msp1/p75 is O-glycosylated in Lactobacillus rhamnosus GG
    Sarah Lebeer
    Centre of Microbial and Plant Genetics, K, U, Leuven, Kasteelpark Arenberg 20, Box 2460, B 3001 Leuven, Belgium
    Microb Cell Fact 11:15. 2012
    ..Nevertheless, knowledge of protein glycosylation holds important potential for better understanding specific glycan-mediated interactions of probiotics and for glycoengineering in food-grade microbes...
  7. pmc Mannose-binding lectin does not explain the course and outcome of pregnancy in rheumatoid arthritis
    Fleur E van de Geijn
    Department of Rheumatology, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 50, NL 3015 GE, Rotterdam, The Netherlands
    Arthritis Res Ther 13:R10. 2011
    ..We also studied the association between MBL genotypes and pregnancy outcomes in RA...
  8. pmc Immunoglobulin G galactosylation and sialylation are associated with pregnancy-induced improvement of rheumatoid arthritis and the postpartum flare: results from a large prospective cohort study
    Fleur E van de Geijn
    Department of Rheumatology, Erasmus MC University Medical Center Rotterdam, Dr, Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Arthritis Res Ther 11:R193. 2009
    ..Since previous studies were small, did not include the postpartum flare and did not study sialylation, these issues were addressed in the present study...
  9. pmc Glycan labeling strategies and their use in identification and quantification
    L R Ruhaak
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300RC Leiden, The Netherlands
    Anal Bioanal Chem 397:3457-81. 2010
    ..In this review, various aspects of glycan labeling, separation, and detection strategies are discussed...
  10. ncbi request reprint Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands
    FEBS J 273:347-61. 2006
    ..Like monomeric LDN and LDNF, the dimeric LDN(F) moieties found here are expected to be targets of humoral and cellular immune responses during schistosome infection...
  11. ncbi request reprint Matrix-assisted laser desorption/ionization in-source decay combined with tandem time-of-flight mass spectrometry of permethylated oligosaccharides: targeted characterization of specific parts of the glycan structure
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Rapid Commun Mass Spectrom 20:943-51. 2006
    ..In conclusion, combination of MALDI-ISD with TOF/TOF-MS allows the detailed characterization of specific moieties of permethylated oligosaccharides and is, therefore, a powerful technique for structural glycomics...
  12. ncbi request reprint Mass spectrometry of proton adducts of fucosylated N-glycans: fucose transfer between antennae gives rise to misleading fragments
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Rapid Commun Mass Spectrom 20:1747-54. 2006
    ..This study indicates that caution should be applied with the interpretation of tandem mass spectrometric (MS/MS) data of protonated glycoconjugates, including glycopeptides, because of the potential occurrence of fucose rearrangements...
  13. ncbi request reprint Glycoproteomics based on tandem mass spectrometry of glycopeptides
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 849:115-28. 2007
    ..This review discusses the potential and limitations of tandem mass spectrometry of glycopeptides as a tool in structural glycoproteomics...
  14. ncbi request reprint Gender-specific expression of complex-type N-glycans in schistosomes
    Manfred Wuhrer
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Glycobiology 16:991-1006. 2006
    ....
  15. doi request reprint Two-dimensional HPLC separation with reverse-phase-nano-LC-MS/MS for the characterization of glycan pools after labeling with 2-aminobenzamide
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Postbus 9600, 2300 RC, Leiden, The Netherlands
    Methods Mol Biol 534:79-91. 2009
    ..In conclusion, the presented methods allow the detailed mass spectrometric characterization of complex N-glycan pools released from various biological sources...
  16. doi request reprint Mass spectrometric glycan rearrangements
    Manfred Wuhrer
    Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden, The Netherlands
    Mass Spectrom Rev 30:664-80. 2011
    ..Hence, tandem mass spectrometric analysis of alkali adducts or deprotonated ions often prevents rearrangement reactions, while they may happen with high efficacy with protonated glycoconjugates...
  17. doi request reprint Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry
    Manfred Wuhrer
    Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, P O Box 9600, 2300 RC Leiden, The Netherlands
    Mass Spectrom Rev 28:192-206. 2009
    ..c) 2009 Wiley Periodicals, Inc., Mass Spec Rev 28:192-206, 2009...
  18. ncbi request reprint Protein glycosylation analysis by liquid chromatography-mass spectrometry
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 825:124-33. 2005
    ..Performed on nano-scale or capillary-scale, these LC-MS methods operate at femtomole sensitivity and support the further integration of glycosylation analysis in proteomics methodology...
  19. ncbi request reprint Glycosylation profiling of immunoglobulin G (IgG) subclasses from human serum
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Proteomics 7:4070-81. 2007
    ..The changes in galactosylation associated with rheumatoid arthritis could readily be monitored. This method is suitable for the subclass-specific analysis of IgG glycosylation from clinical samples...
  20. ncbi request reprint Normal-phase nanoscale liquid chromatography-mass spectrometry of underivatized oligosaccharides at low-femtomole sensitivity
    Manfred Wuhrer
    Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Chem 76:833-8. 2004
    ..The method should be useful for the sensitive and quick analysis of glycosylation patterns and individual oligosaccharides from biotechnologically produced glycoproteins as well as scarcely available biological samples...
  21. ncbi request reprint Glycopeptide analysis by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry reveals novel features of horseradish peroxidase glycosylation
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Rapid Commun Mass Spectrom 18:1741-8. 2004
    ....
  22. doi request reprint Ligand identification of carbohydrate-binding proteins employing a biotinylated glycan binding assay and tandem mass spectrometry
    Manfred Wuhrer
    Department of Parasitology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Anal Biochem 406:132-40. 2010
    ..This approach should be generally applicable to study and structurally identify carbohydrate ligands of anti-glycan antibodies and lectins...
  23. ncbi request reprint Protein glycosylation analyzed by normal-phase nano-liquid chromatography--mass spectrometry of glycopeptides
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Chem 77:886-94. 2005
    ..Thus, the presented, unique combination of Pronase digestion of glycoproteins, normal-phase nano-LC, and multistage MS provides a method for the facile characterization of site-specific protein glycosylation...
  24. ncbi request reprint New features of site-specific horseradish peroxidase (HRP) glycosylation uncovered by nano-LC-MS with repeated ion-isolation/fragmentation cycles
    Manfred Wuhrer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Biochim Biophys Acta 1723:229-39. 2005
    ..The finding of these new features of glycosylation of an already exceptionally well-studied glycoprotein underscores the potential of the nano-LC-MS(n) based analytical approach followed...
  25. doi request reprint Hexose rearrangements upon fragmentation of N-glycopeptides and reductively aminated N-glycans
    Manfred Wuhrer
    Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Chem 81:4422-32. 2009
    ..This characteristic of glycopeptide MS/MS has to be considered when developing tools for de novo glycopeptide structural analysis...
  26. pmc Targeted glycoproteomic analysis reveals that kappa-5 is a major, uniquely glycosylated component of Schistosoma mansoni egg antigens
    Moniek H J Meevissen
    Department of Parasitology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
    Mol Cell Proteomics 10:M110.005710. 2011
    ..mansoni. The distinct structural and antigenic properties of kappa-5 glycans suggest a specific role for kappa-5 in schistosome egg immunogenicity...
  27. doi request reprint Glycoproteomic analysis of human fibrinogen reveals novel regions of O-glycosylation
    Gerhild Zauner
    Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Leiden, The Netherlands
    J Proteome Res 11:5804-14. 2012
    ..Moreover, we describe seven novel O-glycosylation regions in human fibrinogen, all occupied by a monosialylated T-antigen. Our findings may help to improve the general understanding of human fibrinogen in the blood clotting process...
  28. pmc Targeted biomarker discovery by high throughput glycosylation profiling of human plasma alpha1-antitrypsin and immunoglobulin A
    L Renee Ruhaak
    Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
    PLoS ONE 8:e73082. 2013
    ....
  29. doi request reprint MALDI-TOF-MS analysis of sialylated glycans and glycopeptides using 4-chloro-α-cyanocinnamic acid matrix
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Proteomics 12:1337-48. 2012
    ..In conclusion, we clearly demonstrate the high potential of this rationally designed matrix for glycomics and glycoproteomics...
  30. doi request reprint Fc specific IgG glycosylation profiling by robust nano-reverse phase HPLC-MS using a sheath-flow ESI sprayer interface
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    J Proteomics 75:1318-29. 2012
    ....
  31. ncbi request reprint Targeted identification of a unique glycan epitope of Schistosoma mansoni egg antigens using a diagnostic antibody
    Marjolein L M Robijn
    Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, P O Box 9600, 2300 RC Leiden, The Netherlands
    Mol Biochem Parasitol 151:148-61. 2007
    ..This multifucosylated motif has so far only been found in schistosomes, providing a structural basis for the high specificity of the diagnostic antibody...
  32. doi request reprint Structural characterization of glycans on omega-1, a major Schistosoma mansoni egg glycoprotein that drives Th2 responses
    Moniek H J Meevissen
    Department of Parasitology, Leiden University Medical Centre, 2333 ZA Leiden, The Netherlands
    J Proteome Res 9:2630-42. 2010
    ..mansoni glycoprotein. The obtained knowledge of the glycans present on omega-1 contributes to a full understanding of the mode of action of this immunomodulatory glycoprotein...
  33. doi request reprint Plasma protein N-glycan profiles are associated with calendar age, familial longevity and health
    L Renee Ruhaak
    Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Leiden, The Netherlands
    J Proteome Res 10:1667-74. 2011
    ..In conclusion, we describe two glycan features, of which increased levels mark familial longevity and decreased levels of one of these features mark the presence of cardiovascular disease...
  34. doi request reprint IgG glycosylation analysis
    Carolin Huhn
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Proteomics 9:882-913. 2009
    ..Alternatively, intact IgGs or fragments thereof obtained by enzymatic cleavages in the hinge region and by reduction may be analyzed by a large number of analytical techniques, including MS and chromatography or CE...
  35. pmc Glycoproteomic analysis of antibodies
    Gerhild Zauner
    Biomolecular Mass Spectrometry Unit, Postbus 9600, 2300RC Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 12:856-65. 2013
    ..Glycoproteomic analyses of IgM and IgE are lagging behind but should complete our picture of glycosylation's influence on antibody function...
  36. pmc Investigations on aberrant glycosylation of glycosphingolipids in colorectal cancer tissues using liquid chromatography and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS)
    Stephanie Holst
    Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 12:3081-93. 2013
    ..The obtained discriminating glycans can contribute to progress the discovery of biomarkers to improve diagnostics and patient treatment. ..
  37. doi request reprint Robust and high-throughput sample preparation for (semi-)quantitative analysis of N-glycosylation profiles from plasma samples
    L Renee Ruhaak
    Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Leiden, The Netherlands
    Methods Mol Biol 893:371-85. 2012
    ..High throughput is achieved by conducting all sample preparation steps in the 96-well format. The robustness and repeatability of the sample preparation strategy is evaluated...
  38. pmc Fc-glycosylation of IgG1 is modulated by B-cell stimuli
    Jun Wang
    Department of Rheumatology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Mol Cell Proteomics 10:M110.004655. 2011
    ..Our study, therefore, furthers our understanding of the regulation of IgG1 glycosylation at the cellular level...
  39. doi request reprint IgG fc N-glycosylation changes in Lambert-Eaton myasthenic syndrome and myasthenia gravis
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    J Proteome Res 10:143-52. 2011
    ..Interestingly, LEMS patients below 50 years showed elevated levels of bisecting N-acetylglucosamine on IgG1 and IgG2, demonstrating for the first time the link of changes in the level of bisecting N-acetylglucosamine with disease...
  40. doi request reprint Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues
    Cees Bruggink
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    FEBS J 277:2970-86. 2010
    ..The results show the potential and versatility of the analytical approach for structural characterization of oligosaccharides in various body fluids...
  41. doi request reprint Coupling porous sheathless interface MS with transient-ITP in neutral capillaries for improved sensitivity in glycopeptide analysis
    Anthonius A M Heemskerk
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Electrophoresis 34:383-7. 2013
    ..In conclusion, the use of the highly sensitive t-ITP-CZE-MS method will provide information on IgG Fc glycosylation for those samples with IgG1 concentrations below the LODs of the conventional method...
  42. ncbi request reprint Electron transfer dissociation of N-glycopeptides: loss of the entire N-glycosylated asparagine side chain
    M Isabel Catalina
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Rapid Commun Mass Spectrom 21:1053-61. 2007
    ..Thus, our study brings nano-LC/QIT-MS(n) with CID and ETD to the fore as a powerful technique for glycoproteomics at the glycopeptide level...
  43. pmc Pregnancy-associated serum N-glycome changes studied by high-throughput MALDI-TOF-MS
    Bas C Jansen
    Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Sci Rep 6:23296. 2016
    ..In conclusion, our new high-throughput workflow allowed the identification of novel plasma glycosylation changes with pregnancy. ..
  44. doi request reprint Immunoglobulin G glycopeptide profiling by matrix-assisted laser desorption ionization Fourier transform ion cyclotron resonance mass spectrometry
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Chem 82:1073-81. 2010
    ..The method was found suitable for isotype-specific high-throughput IgG glycosylation profiling from human plasma. As an example we successfully applied the method to profile the IgG glycosylation of 62 human samples...
  45. doi request reprint Mapping O-glycosylation of apolipoprotein C-III in MALDI-FT-ICR protein profiles
    Simone Nicolardi
    Center for Proteomics and Metabolomics, Leiden University Medical Center LUMC, Leiden, The Netherlands
    Proteomics 13:992-1001. 2013
    ..ESI-FT-ICR-MS/MS analyses of the same serum samples were performed to confirm the identity of apolipoprotein C-III glycoforms...
  46. doi request reprint Cotton HILIC SPE microtips for microscale purification and enrichment of glycans and glycopeptides
    Maurice H J Selman
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Anal Chem 83:2492-9. 2011
    ..In conclusion, we establish cotton wool microtips for glycan and glycopeptide purification with subsequent mass spectrometric detection...
  47. doi request reprint Hydrophilic interaction chromatography-based high-throughput sample preparation method for N-glycan analysis from total human plasma glycoproteins
    L Renee Ruhaak
    Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, P O Box 9600, 2300RC Leiden, The Netherlands
    Anal Chem 80:6119-26. 2008
    ..Up to four times, 96 human plasma samples can be handled in parallel, which, together with the versatility of the 2-AA label, makes this procedure very attractive for glycomics analysis of larger sample cohorts...
  48. doi request reprint Linkage-Specific in Situ Sialic Acid Derivatization for N-Glycan Mass Spectrometry Imaging of Formalin-Fixed Paraffin-Embedded Tissues
    Stephanie Holst
    Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
    Anal Chem 88:5904-13. 2016
    ....
  49. doi request reprint Protein glycosylation analysis by HILIC-LC-MS of Proteinase K-generated N- and O-glycopeptides
    Gerhild Zauner
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    J Sep Sci 33:903-10. 2010
    ..In conclusion, this approach appears to be a valuable technique for in-depth analysis of the site-specific N-glycosylation and O-glycosylation of individual glycoproteins...
  50. pmc Mass spectrometric identification of aberrantly glycosylated human apolipoprotein C-III peptides in urine from Schistosoma mansoni-infected individuals
    Crina I A Balog
    Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 9:667-81. 2010
    ..Furthermore, our results indicate for the first time that as a result of S. mansoni infection the glycosylation of a host protein is altered...
  51. pmc A novel Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)-core modification attached to the proximal N-acetylglucosamine of keyhole limpet haemocyanin (KLH) N-glycans
    Manfred Wuhrer
    Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Biochem J 378:625-32. 2004
    ..The Gal(beta1-4)Fuc- and Gal(beta1-4)Gal(beta1-4)Fuc- core modifications are expected to be immunogenic, similar to other non-mammalian-type core modifications, and to contribute to the immunostimulatory properties of KLH...
  52. doi request reprint Mass spectrometric O-glycan analysis after combined O-glycan release by beta-elimination and 1-phenyl-3-methyl-5-pyrazolone labeling
    Gerhild Zauner
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Postbus 9600, 2300 RC, Leiden, The Netherlands
    Biochim Biophys Acta 1820:1420-8. 2012
    ..In contrast to N-glycans which are generally enzymatically released for analysis, there is no corresponding enzyme for O-glycan liberation. Therefore, O-glycans are generally released by chemical methods involving tedious procedures...
  53. doi request reprint Protein O-glycosylation analysis
    Gerhild Zauner
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, NL 2300 RC Leiden, The Netherlands
    Biol Chem 393:687-708. 2012
    ..Finally, the O-glycan analytical challenges that need to be met will be discussed...
  54. pmc Decreased levels of bisecting GlcNAc glycoforms of IgG are associated with human longevity
    L Renee Ruhaak
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    PLoS ONE 5:e12566. 2010
    ..It has been shown previously that the N-glycosylation pattern of human immunoglobulin G (IgG) is age-dependent. Here we investigate whether N-linked glycans reflect early features of human longevity...
  55. ncbi request reprint Glycomics-driven discoveries in schistosome research
    Cornelis H Hokke
    Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Exp Parasitol 117:275-83. 2007
    ..Here we will present a concise review of the already available data guided by a selection of recently generated stage-specific glycan profiles, and discuss implications and prospects of glycomics studies of schistosomes...
  56. pmc Classification for Longevity Potential: The Use of Novel Biomarkers
    Marian Beekman
    Molecular Epidemiology, Leiden University Medical Center, Leiden, Netherlands
    Front Public Health 4:233. 2016
    ..In the current paper, we aim to classify a group of older people into those with longevity potential or controls...
  57. doi request reprint MassyTools: A High-Throughput Targeted Data Processing Tool for Relative Quantitation and Quality Control Developed for Glycomic and Glycoproteomic MALDI-MS
    Bas C Jansen
    Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    J Proteome Res 14:5088-98. 2015
    ..The package is released under the Apache 2.0 license and is freely available on GitHub ( https://github.com/Tarskin/MassyTools )...
  58. pmc Immunoglobulin G (IgG) Fab glycosylation analysis using a new mass spectrometric high-throughput profiling method reveals pregnancy-associated changes
    Albert Bondt
    From the Department of Rheumatology, Erasmus University Medical Center, 3000 CA Rotterdam, The Netherlands Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
    Mol Cell Proteomics 13:3029-39. 2014
    ....
  59. doi request reprint Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis
    Yoann Rombouts
    Department of Rheumatology, Leiden University Medical Centre, Leiden, The Netherlands
    Ann Rheum Dis 74:234-41. 2015
    ..To determine whether anticitrullinated protein antibodies (ACPA) exhibit specific changes in Fc glycosylation prior to the onset of arthritis...
  60. pmc N-glycosylation of colorectal cancer tissues: a liquid chromatography and mass spectrometry-based investigation
    Crina I A Balog
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 11:571-85. 2012
    ....
  61. doi request reprint Site-specific N-glycosylation analysis of human immunoglobulin e
    Rosina Plomp
    Center for Proteomics and Metabolomics, Department of Rheumatology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    J Proteome Res 13:536-46. 2014
    ..Our approach allows comparison of the glycosylation of IgE samples in a site-specific manner. ..
  62. doi request reprint Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis
    Gerhild Zauner
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Methods Mol Biol 951:113-27. 2013
    ..Using this approach, site-specific information on protein glycosylation is obtained...
  63. doi request reprint 2-picoline-borane: a non-toxic reducing agent for oligosaccharide labeling by reductive amination
    L Renee Ruhaak
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Proteomics 10:2330-6. 2010
    ..Therefore, 2-picoline-borane is a non-toxic alternative to sodium cyanoborohydride for the labeling of oligosaccharides...
  64. doi request reprint Optimized workflow for preparation of APTS-labeled N-glycans allowing high-throughput analysis of human plasma glycomes using 48-channel multiplexed CGE-LIF
    L Renee Ruhaak
    Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Leiden, The Netherlands
    J Proteome Res 9:6655-64. 2010
    ..Alterations in the glycosylation patterns were observed with gestation and time after delivery...
  65. pmc Human plasma protein N-glycosylation
    Florent Clerc
    Center for Proteomics and Metabolomics, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Glycoconj J 33:309-43. 2016
    ..In the discussion, we put the glycosylation of individual proteins into perspective and speculate how the individual proteins may contribute to a total plasma N-glycosylation profile determined at the released glycan level. ..
  66. pmc Acute phase inflammation is characterized by rapid changes in plasma/peritoneal fluid N-glycosylation in mice
    Yoann Rombouts
    Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
    Glycoconj J 33:457-70. 2016
    ..Importantly, substantial changes in glycosylation were already observed 12 h after induction of peritonitis, thereby demonstrating an unexpected velocity of the biological mechanisms involved. ..
  67. doi request reprint Regulated glycosylation patterns of IgG during alloimmune responses against human platelet antigens
    Manfred Wuhrer
    Department of Parasitology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands m wuhrer
    J Proteome Res 8:450-6. 2009
    ..Moreover, experiments in animal models as well as in vitro immunological tests will be needed to unravel the mechanisms regulating IgG Fc glycosylation...
  68. pmc Changes in antigen-specific IgG1 Fc N-glycosylation upon influenza and tetanus vaccination
    Maurice H J Selman
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 11:M111.014563. 2012
    ..Our data indicate that dependent on the vaccination time point the infectious agent will encounter IgGs with different glycosylation profiles, which are expected to influence the antibody effector functions relevant in immunity...
  69. doi request reprint Microarray technology using glycans extracted from natural sources for serum antibody fluorescent detection
    Emanuela Lonardi
    Department of Parasitology, Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Center for Infectious Diseases, Leiden, The Netherlands
    Methods Mol Biol 808:285-302. 2012
    ..This approach may be used to detect autoantibodies in cancer as well as in autoimmune diseases...
  70. pmc Analysis of urinary oligosaccharides in lysosomal storage disorders by capillary high-performance anion-exchange chromatography-mass spectrometry
    Cees Bruggink
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Anal Bioanal Chem 403:1671-83. 2012
    ..In conclusion, using online coupling of HPAEC with mass spectrometric detection, our study provides characteristic urinary oligosaccharide fingerprints with diagnostic potential for lysosomal storage disorders...
  71. ncbi request reprint General microarray technique for immobilization and screening of natural glycans
    Arjen R de Boer
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Anal Chem 79:8107-13. 2007
    ..Moreover, it was applied for the characterization of the microarray glycans by using known carbohydrate-binding proteins...
  72. doi request reprint Natural glycan microarrays
    Emanuela Lonardi
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, PO Box 9600, 2300 RC Leiden, The Netherlands
    Expert Rev Proteomics 7:761-74. 2010
    ..The importance of glycan density and multivalency is discussed. Furthermore, some applications of natural glycan microarrays for studying lectin and antibody binding are presented...
  73. doi request reprint Glycan profiling of anti-citrullinated protein antibodies isolated from human serum and synovial fluid
    Hans U Scherer
    Leiden University Medical Center, Leiden, The Netherlands
    Arthritis Rheum 62:1620-9. 2010
    ..We undertook this study to analyze Fc glycosylation of IgG1 ACPA in serum and synovial fluid (SF) in order to further characterize the immune response to citrullinated antigens...
  74. doi request reprint Skewed Fc glycosylation profiles of anti-proteinase 3 immunoglobulin G1 autoantibodies from granulomatosis with polyangiitis patients show low levels of bisection, galactosylation, and sialylation
    Manfred Wuhrer
    Center for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    J Proteome Res 14:1657-65. 2015
    ..Total IgG1 and anti-PR3 IgG1 Fc galactosylation were weakly correlated, while bisection of IgG1 and anti-PR3 showed no correlation. Our data indicate that aberrant ANCA galactosylation may be driven in an antigen-specific manner. ..
  75. doi request reprint Fibrinogen alpha chain O-glycopeptides as possible markers of urinary tract infection
    Tiziana Pacchiarotta
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    J Proteomics 75:1067-73. 2012
    ....
  76. doi request reprint Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics
    Gerhild Zauner
    Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Electrophoresis 32:3456-66. 2011
    ....
  77. doi request reprint Oligosaccharide analysis by graphitized carbon liquid chromatography-mass spectrometry
    L Renee Ruhaak
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P O Box 9600, 2300, RC, Leiden, The Netherlands
    Anal Bioanal Chem 394:163-74. 2009
    ..While graphitized carbon HPLC-MS for glycan analysis is still only applied by a limited number of groups, more users are expected to apply this method when databases which support structural assignment become available...
  78. ncbi request reprint Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry
    Cees Bruggink
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center for Infectious Diseases, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 829:136-43. 2005
    ..Isomeric glycans in the sample could be resolved by HPAEC and assigned on the basis of the monosaccharide linkage information revealed by on-line IT-MS/MS...
  79. doi request reprint Alignment of laser-induced fluorescence and mass spectrometric detection traces using electrophoretic mobility scaling in CE-LIF-MS of labeled N-glycans
    Carolin Huhn
    Forschungszentrum Julich, Central Division of Analytical Chemistry, Julich, Germany
    Electrophoresis 33:563-6. 2012
    ..As an example, the proposed methodology is applied to a mixture of labeled as well as non-labeled N-glycans...
  80. doi request reprint Hexapeptide library as a universal tool for sample preparation in protein glycosylation analysis
    Carolin Huhn
    Forschungszentrum Julich, Central Division of Analytical Chemistry, Julich, Germany
    J Proteomics 75:1515-28. 2012
    ..Instead, fractionation techniques or profiling strategies on the glycopeptide level after enrichment are proposed for in-depth glycoproteomics research...
  81. doi request reprint Immunoglobulin 1 (IgG1) Fc-glycosylation profiling of anti-citrullinated peptide antibodies from human serum
    H Ulrich Scherer
    Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Berlin, Germany
    Proteomics Clin Appl 3:106-15. 2009
    ..Using this method, studies can be performed on the biological and clinical relevance of ACPA glycosylation within RA patient cohorts...
  82. ncbi request reprint Schistosoma mansoni and Schistosoma haematobium: identification and characterization of glycoconjugate antigens in the hemolymph of infected vector snails
    Jürgen Schmitt
    Department of Tropical Hygiene and Public Health, University of Heidelberg, Germany
    J Parasitol 88:505-13. 2002
    ..Removal of terminal fucose abolished reactivity with both monoclonal antibodies. Both glycans are different from previously characterized schistosome carbohydrates. Their biological function(s) remain to be defined...
  83. pmc Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity
    Sven R Kantelhardt
    Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D 35392 Giessen, Germany
    Biochem J 366:217-23. 2002
    ..The Fuc(alpha1-->3)GalNAc-motif also appeared to be the basis for the cross-reactivity with KLH, a phenomenon used in the serodiagnosis of S. mansoni, S. haematobium and S. japonicum infections...
  84. ncbi request reprint Characterization of glycosphingolipids from Schistosoma mansoni eggs carrying Fuc(alpha1-3)GalNAc-, GalNAc(beta1-4)[Fuc(alpha1-3)]GlcNAc- and Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc- (Lewis X) terminal structures
    Manfred Wuhrer
    Institute of Biochemistry, University of Giessen, Germany
    Eur J Biochem 269:481-93. 2002
    ....
  85. ncbi request reprint Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(beta1-6)Man-motifs
    Tomofumi Kurokawa
    Pharmaceutical Discovery Center, Pharmaceutical Research Division, Takeda Chemical Industries, Ltd, Osaka, Japan
    Eur J Biochem 269:5459-73. 2002
    ..Hence, our studies demonstrate that this marine mollusk glycoprotein is characterized by a unique oligosaccharide pattern comprising, in part, novel structural elements...
  86. ncbi request reprint Glycosylation profiling in clinical proteomics: heading for glycan biomarkers
    Manfred Wuhrer
    Expert Rev Proteomics 4:135-6. 2007
  87. ncbi request reprint Molecular characterization of omega-1: a hepatotoxic ribonuclease from Schistosoma mansoni eggs
    Colin M Fitzsimmons
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Mol Biochem Parasitol 144:123-7. 2005
  88. ncbi request reprint Identification and characterization of keyhole limpet hemocyanin N-glycans mediating cross-reactivity with Schistosoma mansoni
    Hildegard Geyer
    Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D 35392 Giessen, Germany
    J Biol Chem 280:40731-48. 2005
    ..This study provides for the first time detailed structural data on the KLH carbohydrate entities responsible for cross-reactivity with glycoconjugates from S. mansoni...
  89. ncbi request reprint The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal(beta1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity
    Manfred Wuhrer
    Institute of Biochemistry, Medical Faculty, University of Giessen, D 35392 Giessen, Germany
    Glycobiology 14:115-26. 2004
    ..granulosus and Taenia crassiceps neogala-glycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections...
  90. ncbi request reprint Localization of defined carbohydrate epitopes in bovine polysialylated NCAM
    Manfred Wuhrer
    Biochemisches Institut, Fachbereich Humanmedizin, Friedrichstrasse 24, Universitat Giessen, 35392 Giessen, Germany
    Biochimie 85:207-18. 2003
    ..When compared with previous data on murine NCAM glycosylation, our results indicate a conservation of structural features and attachment sites for the different types of NCAM N-glycans...
  91. ncbi request reprint A novel GlcNAcalpha1-HPO3-6Gal(1-1)ceramide antigen and alkylated inositol-phosphoglycerolipids expressed by the liver fluke Fasciola hepatica
    Manfred Wuhrer
    Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D 35392 Giessen, Germany
    Glycobiology 13:129-37. 2003
    ..hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcalpha1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections...
  92. ncbi request reprint Characterization of keyhole limpet hemocyanin (KLH) glycans sharing a carbohydrate epitope with Schistosoma mansoni glycoconjugates
    Hildegard Geyer
    Faculty of Medicine, Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, Giessen D 35392, Germany
    Micron 35:105-6. 2004
    ..Oligosaccharides cross-reacting with schistosomal glycans comprised a terminal Fuc(alpha1-3)GalNAc-motif, which appears to represent the main carbohydrate epitope mediating cross-reactivity of KLH with glycoconjugates from S. mansoni...
  93. ncbi request reprint Distinct contributions of beta 4GalNAcTA and beta 4GalNAcTB to Drosophila glycosphingolipid biosynthesis
    Anita Stolz
    Zelluläre Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, 30625, Hannover, Germany
    Glycoconj J 25:167-75. 2008
    ....
  94. ncbi request reprint Localization and characterization of polysialic acid-containing N-linked glycans from bovine NCAM
    Maren von der Ohe
    Zentrum für Molekulare Neurobiologie, University of Hamburg, D 20246 Hamburg, Germany
    Glycobiology 12:47-63. 2002
    ..In conclusion, we observed a structural variability and high regional selectivity for the PSA-glycans attached to the NCAM molecule that are most likely influencing its biological functions...
  95. ncbi request reprint Efficient introduction of a bisecting GlcNAc residue in tobacco N-glycans by expression of the gene encoding human N-acetylglucosaminyltransferase III
    Gerard J A Rouwendal
    Business Unit Bioscience, Plant Research International BV, Wageningen University and Research Centre, Droevendaalsesteeg 1 6708 PB Wageningen, The Netherlands
    Glycobiology 17:334-44. 2007
    ..Moreover, we have shown that the majority of N-glycans of an antibody produced in a plant expressing GnT-III is also bisected. This might improve the efficacy of therapeutic antibodies produced in this type of transgenic plant...
  96. ncbi request reprint Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin
    Julie Bouckaert
    Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel and Vlaams Interuniversitair Instituut voor Biotechnologie, Building E, Pleinlaan 2, 1050 Brussels, Belgium
    Mol Microbiol 55:441-55. 2005
    ..coli. We foresee that our findings will spark new ideas and initiatives for the development of UTI vaccines and anti-adhesive drugs to prevent anticipated and recurrent UTIs...