Maw Sheng Chien

Summary

Affiliation: National Chung Hsing University
Country: Taiwan

Publications

  1. pmc Mutations in the Salmonella enterica serovar Choleraesuis cAMP-receptor protein gene lead to functional defects in the SPI-1 Type III secretion system
    Zeng Weng Chen
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, Republic of China
    Vet Res 41:5. 2010
  2. ncbi request reprint Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs
    Chih Ming Liao
    Graduate Institute of Veterinary Pathology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vaccine 24:27-35. 2006
  3. pmc Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages
    Zeng Weng Chen
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250, Kuo Kuang Road, Taichung, 402, Taiwan, ROC
    Vet Res 42:25. 2011
  4. ncbi request reprint Yeast-expressed classical swine fever virus glycoprotein E2 induces a protective immune response
    Guang Jan Lin
    Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vet Microbiol 139:369-74. 2009
  5. doi request reprint Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages
    Maw Sheng Chien
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 402, Taiwan
    Vet Microbiol 135:327-33. 2009
  6. doi request reprint Yeast expressed classical swine fever E2 subunit vaccine candidate provides complete protection against lethal challenge infection and prevents horizontal virus transmission
    Guang Jan Lin
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vaccine 30:2336-41. 2012
  7. doi request reprint Characterization of the monoclonal antibody against classical swine fever virus glycoprotein E(rns) and its application to an indirect sandwich ELISA
    Ching Wei Wu
    Graduate Institute of Microbiology and Public Health College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, Republic of China
    Appl Microbiol Biotechnol 92:815-21. 2011
  8. ncbi request reprint Expression and immunological studies of classical swine fever virus glycoprotein E2 in the bi-cistronic baculovirus/larvae expression system
    Chi Ming Wu
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
    Biosci Biotechnol Biochem 74:1343-9. 2010
  9. doi request reprint Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development
    Pei Ching Wu
    College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227 Taiwan, Republic of China
    Appl Microbiol Biotechnol 95:1501-7. 2012
  10. doi request reprint Mitogen-activated protein kinases p38 and JNK mediate Actinobacillus pleuropneumoniae exotoxin ApxI-induced apoptosis in porcine alveolar macrophages
    Chi Ming Wu
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, ROC
    Vet Microbiol 151:372-8. 2011

Collaborators

Detail Information

Publications28

  1. pmc Mutations in the Salmonella enterica serovar Choleraesuis cAMP-receptor protein gene lead to functional defects in the SPI-1 Type III secretion system
    Zeng Weng Chen
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, Republic of China
    Vet Res 41:5. 2010
    ....
  2. ncbi request reprint Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs
    Chih Ming Liao
    Graduate Institute of Veterinary Pathology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vaccine 24:27-35. 2006
    ..Our findings indicate these non-toxic rsPMT proteins are attractive candidates for development of a subunit vaccine against PAR in pigs...
  3. pmc Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages
    Zeng Weng Chen
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250, Kuo Kuang Road, Taichung, 402, Taiwan, ROC
    Vet Res 42:25. 2011
    ..Collectively, this study demonstrated ApxI induces gene expression of IL-1β, IL-8 and TNF-α in PAMs that involves β2 integrins and downstream MAPKs...
  4. ncbi request reprint Yeast-expressed classical swine fever virus glycoprotein E2 induces a protective immune response
    Guang Jan Lin
    Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vet Microbiol 139:369-74. 2009
    ..The yeast-expressed E2 protein retains correct immunogenicity and is able to induce a protective immune response against CSFV infection...
  5. doi request reprint Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages
    Maw Sheng Chien
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 402, Taiwan
    Vet Microbiol 135:327-33. 2009
    ..These findings suggest a role of ApxI in AP infection as it might impair the host defense system through the induction of apoptosis in PAMs...
  6. doi request reprint Yeast expressed classical swine fever E2 subunit vaccine candidate provides complete protection against lethal challenge infection and prevents horizontal virus transmission
    Guang Jan Lin
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vaccine 30:2336-41. 2012
    ..All the sentinel pigs were alive and had no clinical symptoms confirming yE2 vaccine could confer a protective immune response and prevent horizontal transmission of CSFV...
  7. doi request reprint Characterization of the monoclonal antibody against classical swine fever virus glycoprotein E(rns) and its application to an indirect sandwich ELISA
    Ching Wei Wu
    Graduate Institute of Microbiology and Public Health College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, Republic of China
    Appl Microbiol Biotechnol 92:815-21. 2011
    ..The assay demonstrated a high sensitivity and specificity that may provide an alternative method for developing a diagnostic kit with easy manipulation and low cost...
  8. ncbi request reprint Expression and immunological studies of classical swine fever virus glycoprotein E2 in the bi-cistronic baculovirus/larvae expression system
    Chi Ming Wu
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
    Biosci Biotechnol Biochem 74:1343-9. 2010
    ..This is the first study to indicate that baculovirus/T. ni larvae-expressed rE2 can be served as a vaccine candidate. This system provides an economical alternative for the production of vaccine components in the veterinary industry...
  9. doi request reprint Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development
    Pei Ching Wu
    College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227 Taiwan, Republic of China
    Appl Microbiol Biotechnol 95:1501-7. 2012
    ..The Cap1-233-immunized pigs demonstrated specific antibody immune responses and are prevented from PCV2 challenge, thus implying its potential use for a VLP-based PCV2 vaccine...
  10. doi request reprint Mitogen-activated protein kinases p38 and JNK mediate Actinobacillus pleuropneumoniae exotoxin ApxI-induced apoptosis in porcine alveolar macrophages
    Chi Ming Wu
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, ROC
    Vet Microbiol 151:372-8. 2011
    ..This study is the first to demonstrate that ApxI-induced apoptosis of PAMs involves the activation of p38 and JNK, and engages the extrinsic and intrinsic apoptotic pathways...
  11. doi request reprint Efficacy of a novel Pasteurella multocida vaccine against progressive atrophic rhinitis of swine
    Shih Ling Hsuan
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, ROC
    Vaccine 27:2923-9. 2009
    ..Collectively, these results indicate that the rsPMT-PM vaccine could be used to provide protective immunity for controlling the prevalence and severity of PAR among farm-raised swine...
  12. ncbi request reprint Effects of sugar cane extract on the modulation of immunity in pigs
    Dan Yuan Lo
    Graduate Institute of Veterinary Pathology, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, R O C
    J Vet Med Sci 67:591-7. 2005
    ..Moreover, SCE-treated pigs showed a 7.87% growth enhancement compared with untreated controls. Thus SCE produces an immunostimulative effect on porcine innate immunity that may provide protection against pathogens...
  13. pmc Elucidating the role of ApxI in hemolysis and cellular damage by using a novel apxIA mutant of Actinobacillus pleuropneumoniae serotype 10
    Nai Yun Chang
    Graduate Institutes of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan, R O C
    J Vet Sci 15:81-9. 2014
    ..Collectively, ApxI, but not other components in the exotoxin preparation of A. pleuropneumoniae serotype 10, was responsible for the hemolytic and cytotoxic effects on porcine erythrocytes and alveolar macrophages. ..
  14. doi request reprint Immunostimulation of sugar cane extract on neutrophils to Salmonella typhimurium infection in mice
    Ming Hua Chen
    Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, 250 Kuo Kuang Rd, 40227 Taichung, Taiwan, ROC
    Phytother Res 26:1062-7. 2012
    ..In conclusion, our results suggest that SCE has a positive regulatory effect on the biological function of mouse neutrophils that may increase host resistance against bacterial infections...
  15. ncbi request reprint Secreted expression of the classical swine fever virus glycoprotein E(rns) in yeast and application to a sandwich blocking ELISA
    Chienjin Huang
    Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    J Virol Methods 132:40-7. 2006
    ..The yE(rns) which possesses enzymatic activity and retains antigenicity may provide a useful material for developing a diagnostic kit...
  16. pmc Epidemiologic relationship between fluoroquinolone-resistant Salmonella enterica Serovar Choleraesuis strains isolated from humans and pigs in Taiwan (1997 to 2002)
    Chao Chin Chang
    Graduate Instituteof Veterinary Public Health, National Chung Hsing University, Taichung 402, Taiwan
    J Clin Microbiol 43:2798-804. 2005
    ....
  17. doi request reprint Enhancing expression of the classical swine fever virus glycoprotein E2 in yeast and its application to a blocking ELISA
    Chih Yuan Cheng
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    J Biotechnol 174:1-6. 2014
    ..The recombinant yE2 subunits may provide potential subunit vaccine candidates and useful diagnostic reagents for CSFV with easy manipulation and low cost. ..
  18. ncbi request reprint Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses
    Chienjin Huang
    Graduate Institute of Veterinary Microbiology, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Vet Microbiol 101:209-14. 2004
    ..The relative efficiency (compared to performing separate assays for each virus) and apparent sensitivity of mPCR suggest its potential application for routine molecular diagnostic purposes...
  19. ncbi request reprint Effect of immunostimulation by detoxified E. coli lipopolysaccharide combined with inactivated Propionibacterium granulosum cells on porcine immunity
    Dan Yuan Lo
    College of Veterinary Medicine, National Chung Hsing University, Taichung, Republic of China
    J Vet Med Sci 71:897-903. 2009
    ..8% growth enhancement compared with untreated pigs. Thus, PG/LPS caused positive immunoregulation of porcine innate immune system effectors, non-specific activation of lymphocytes and antibody production...
  20. ncbi request reprint Mapping of functional regions conferring nuclear localization and RNA-binding activity of pseudorabies virus early protein UL54
    Ya Ju Huang
    Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    J Virol Methods 130:102-7. 2005
    ..The utmost N-terminal 83 residues portion of UL54 contains two important functional domains, NLS and RNA-binding, and thus it would play an indispensable role in UL54 regulatory function...
  21. ncbi request reprint Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA
    Pei Ching Wu
    Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    J Biotechnol 133:58-64. 2008
    ....
  22. ncbi request reprint Characterization of the swine U6 promoter for short hairpin RNA expression and its application to inhibition of virus replication
    Ching Wei Wu
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    J Biotechnol 168:78-84. 2013
    ..Furthermore, the antiviral effect of shRNA targeting the classical swine fever virus (CSFV) NS5B driven by the swine U6 promoter was confirmed by the significant reduction of virus replication. ..
  23. doi request reprint Spontaneous rhabdomyosarcoma in a young Sprague-Dawley rat
    Shih Chieh Chang
    Department of Veterinary Medicine and Veterinary Medical Teaching Hospital, National Chung Hsing University, Taichung, Taiwan, ROC
    Toxicol Pathol 36:866-70. 2008
    ..Based on the pathology results, a spontaneous rhabdomyosarcoma with pleomorphic type was diagnosed in a young SD rat...
  24. ncbi request reprint The preparation of porcine circovirus type 2 (PCV2) virus-like particles using a recombinant pseudorabies virus and its application to vaccine development
    Jyun Ni Chi
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    J Biotechnol 181:12-9. 2014
    ..The immunization of mice or guinea pigs with purified VLPs could induce significant, specific antibody responses to PCV2 Cap. These results demonstrate an alternative to PCV2 for the development of a VLP-based subunit vaccine. ..
  25. pmc The Porcine Circovirus Type 2 Nonstructural Protein ORF3 Induces Apoptosis in Porcine Peripheral Blood Mononuclear Cells
    Wei Li Lin
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan
    Open Virol J 5:148-53. 2011
    ....
  26. doi request reprint The N-terminus of porcine circovirus type 2 replication protein is required for nuclear localization and ori binding activities
    Wei Li Lin
    Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC
    Biochem Biophys Res Commun 379:1066-71. 2009
    ..The ability of the utmost 20 residues of the N-terminal region to target the GFP to the nucleus confirmed its role as a functional NLS...
  27. doi request reprint The cytotoxicity to leukemia cells and antiviral effects of Isatis indigotica extracts on pseudorabies virus
    Shih Ling Hsuan
    Graduate Institute of Veterinary Pathobiology and Department of Veterinary Medicine, National Chung Hsing University, 250, Kuo Kuang Road, Taichung, 402, Taiwan, ROC
    J Ethnopharmacol 123:61-7. 2009
    ..The aim of this study was to evaluate the cytotoxicity of Isatis indigotica extracts on human leukemia cell line (HL-60) and the antiviral activity on swine pseudorabies virus (PrV) in in vitro assays...
  28. ncbi request reprint Complete sequence of a subgroup 3.4 strain of classical swine fever virus from Taiwan
    Yu Ju Lin
    Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kou Kwang Road, Taichung 402, Taiwan
    Virus Genes 35:737-44. 2007
    ..Strain 94.4/IL/94/TWN is the first completely genomic sequence of subgroup 3.4 viruses...