You Di Liao

Summary

Affiliation: Academia Sinica
Country: Taiwan

Publications

  1. pmc Purification and cloning of cytotoxic ribonucleases from Rana catesbeiana (bullfrog)
    Y D Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    Nucleic Acids Res 28:4097-104. 2000
  2. pmc Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase
    You Di Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan 115
    Protein Sci 13:1802-10. 2004
  3. pmc The structural integrity exerted by N-terminal pyroglutamate is crucial for the cytotoxicity of frog ribonuclease from Rana pipiens
    You Di Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan
    Nucleic Acids Res 31:5247-55. 2003
  4. ncbi request reprint Solution structure of the cytotoxic RNase 4 from oocytes of bullfrog Rana catesbeiana
    Chun Hua Hsu
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, ROC
    J Mol Biol 326:1189-201. 2003
  5. ncbi request reprint Residues involved in the catalysis, base specificity, and cytotoxicity of ribonuclease from Rana catesbeiana based upon mutagenesis and X-ray crystallography
    Ying Jen Leu
    Institute of Biomedical Sciences and the Institute of Molecular Biology, Academia Sinica, Taipei 115, and the Department of Life Science, National Tsing Hua University, Hsin Chu 300, Taiwan
    J Biol Chem 278:7300-9. 2003
  6. ncbi request reprint Roles of N-terminal pyroglutamate in maintaining structural integrity and pKa values of catalytic histidine residues in bullfrog ribonuclease 3
    Yuan Chao Lou
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, ROC
    J Mol Biol 355:409-21. 2006
  7. doi request reprint NMR and biophysical elucidation of structural effects on extra N-terminal methionine residue of recombinant amphibian RNases from Rana catesbeiana
    Chun Hua Hsu
    Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan
    J Biochem 148:209-15. 2010
  8. pmc Regulation of ribonuclease expression by estradiol in Rana catesbeiana (Bullfrog)
    Pin Chi Tang
    Institute of Biomedical Sciences, Academia Sinica, 128, Yen Chiu Yuan Road, Sec 2, Taipei 115, Taiwan
    Nucleic Acids Res 30:3286-93. 2002
  9. pmc Outer membrane protein I of Pseudomonas aeruginosa is a target of cationic antimicrobial peptide/protein
    Yu Min Lin
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan
    J Biol Chem 285:8985-94. 2010
  10. doi request reprint Solution structure and base specificity of cytotoxic RC-RNase 2 from Rana catesbeiana
    Chun Hua Hsu
    Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan Genome and Systems Biology Degree Program, Center for Systems Biology, National Taiwan University, Taipei 10617, Taiwan Electronic address
    Arch Biochem Biophys 584:70-8. 2015

Collaborators

  • Chinpan Chen
  • Chun Hua Hsu
  • Ming Jing Hwang
  • Tsai Ling Lauderdale
  • Huey Chung Huang
  • Shang Te Danny Hsu
  • Ting Wei Chang
  • Yu Min Lin
  • Tien Sheng Tseng
  • Yu Chie Huang
  • Heng Li Chen
  • Chiu Feng Wang
  • Shih Jung Wu
  • Margaret Dah Tsyr Chang
  • Yuan Chao Lou
  • Shih Han Wang
  • Hung Mu Wei
  • Yu June Wang
  • Ying Jen Leu
  • Keng Chang Tsai
  • Iren Wang
  • Sui Chi Wang
  • Pin Chi Tang
  • Szu Yao Wu
  • Hui Ming Yu
  • Chiaho Shih
  • Ya Shu Chang
  • Pei Yi Su
  • Kaichih Chang
  • Ching Shu Suen
  • Yuan Tsong Chen
  • Shih Hsiung Wu
  • Yan Shin Lee
  • Yun Ru Pan
  • Shuenn Shing Chern
  • Yen Chywan Liaw
  • Imameddin Amiraslanov
  • Ya Yun Hsiao
  • Jen Chong Jeng

Detail Information

Publications14

  1. pmc Purification and cloning of cytotoxic ribonucleases from Rana catesbeiana (bullfrog)
    Y D Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    Nucleic Acids Res 28:4097-104. 2000
    ..Their DNA sequences and amino acid sequences are homologous to those of mammalian ribonuclease superfamily, approximately 50 and approximately 25%, respectively...
  2. pmc Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase
    You Di Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan 115
    Protein Sci 13:1802-10. 2004
    ..On the basis of N-terminal sequence information in the protein database, 85%-90% of recombinant proteins should be produced in authentic form by our engineered MetAPs...
  3. pmc The structural integrity exerted by N-terminal pyroglutamate is crucial for the cytotoxicity of frog ribonuclease from Rana pipiens
    You Di Liao
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan
    Nucleic Acids Res 31:5247-55. 2003
    ..The cytotoxicity of onconase is largely determined by structural integrity and specific catalytic activity for UG through Pyr1, rather than non-specific activity for total RNAs...
  4. ncbi request reprint Solution structure of the cytotoxic RNase 4 from oocytes of bullfrog Rana catesbeiana
    Chun Hua Hsu
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, ROC
    J Mol Biol 326:1189-201. 2003
    ..Furthermore, the differences of side-chain conformations of subsite residues among RNase A, ONC and rRNase 4 are related to their distinct catalytic activities and base preferences...
  5. ncbi request reprint Residues involved in the catalysis, base specificity, and cytotoxicity of ribonuclease from Rana catesbeiana based upon mutagenesis and X-ray crystallography
    Ying Jen Leu
    Institute of Biomedical Sciences and the Institute of Molecular Biology, Academia Sinica, Taipei 115, and the Department of Life Science, National Tsing Hua University, Hsin Chu 300, Taiwan
    J Biol Chem 278:7300-9. 2003
    ..Interestingly, Pyr-1 along with Lys-95 and Glu-97 form four hydrogen bonds with guanine at B(2) site and determine B(2) base specificity...
  6. ncbi request reprint Roles of N-terminal pyroglutamate in maintaining structural integrity and pKa values of catalytic histidine residues in bullfrog ribonuclease 3
    Yuan Chao Lou
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, ROC
    J Mol Biol 355:409-21. 2006
    ....
  7. doi request reprint NMR and biophysical elucidation of structural effects on extra N-terminal methionine residue of recombinant amphibian RNases from Rana catesbeiana
    Chun Hua Hsu
    Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan
    J Biochem 148:209-15. 2010
    ..The structural integrity exerted by the N-terminal Pyr(1) residue may be crucial for amphibian RNases and the greatest structural differences occur on the network of the Pyr(1) residue and S2 beta-sheet region...
  8. pmc Regulation of ribonuclease expression by estradiol in Rana catesbeiana (Bullfrog)
    Pin Chi Tang
    Institute of Biomedical Sciences, Academia Sinica, 128, Yen Chiu Yuan Road, Sec 2, Taipei 115, Taiwan
    Nucleic Acids Res 30:3286-93. 2002
    ..These results support the hypothesis that RC-RNase is synthesized in the liver upon stimulation by estradiol during oogenesis, then secreted into the bloodstream and stored in oocytes for embryonic development...
  9. pmc Outer membrane protein I of Pseudomonas aeruginosa is a target of cationic antimicrobial peptide/protein
    Yu Min Lin
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan
    J Biol Chem 285:8985-94. 2010
    ..Our findings highlight a novel mechanism of antimicrobial activity and document a previously unexplored target of alpha-helical cationic AMPs, which may be used for screening drugs to treat antibiotic-resistant bacterial infection...
  10. doi request reprint Solution structure and base specificity of cytotoxic RC-RNase 2 from Rana catesbeiana
    Chun Hua Hsu
    Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan Genome and Systems Biology Degree Program, Center for Systems Biology, National Taiwan University, Taipei 10617, Taiwan Electronic address
    Arch Biochem Biophys 584:70-8. 2015
    ..Furthermore, the substrate-related residues in the base specificity among native RC-RNases are derived using the chemical shift perturbation on ligand binding. ..
  11. pmc Sarkosyl-Induced Helical Structure of an Antimicrobial Peptide GW-Q6 Plays an Essential Role in the Binding of Surface Receptor OprI in Pseudomonas aeruginosa
    Tien Sheng Tseng
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    PLoS ONE 11:e0164597. 2016
    ..Taken together, our study provides mechanistic insights into the mode of action of the GW-Q6 peptide and suggests its applicability in modifying and developing potent AMPs as therapeutic agents...
  12. pmc Key Residues of Outer Membrane Protein OprI Involved in Hexamer Formation and Bacterial Susceptibility to Cationic Antimicrobial Peptides
    Ting Wei Chang
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    Antimicrob Agents Chemother 59:6210-22. 2015
    ..Our results showed that OprI on bacterial surfaces is responsible for the recruitment and susceptibility to amphipathic α-helical AMPs and may be used to screen antimicrobials. ..
  13. ncbi request reprint The flexible and clustered lysine residues of human ribonuclease 7 are critical for membrane permeability and antimicrobial activity
    Yu Chie Huang
    Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Institute of Biochemical Sciences, National Taiwan University, Taipei 106 Taiwan
    J Biol Chem 282:4626-33. 2007
    ..The conformation of hRNase7 can be adapted for pore formation or disruption of bacterial membrane even at 4 degrees C...
  14. pmc Identification of a novel antimicrobial peptide from human hepatitis B virus core protein arginine-rich domain (ARD)
    Heng Li Chen
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    PLoS Pathog 9:e1003425. 2013
    ..Our current results suggested that HBc ARD could be a new promising antimicrobial peptide...