Patrick Micke

Summary

Affiliation: Uppsala University
Country: Sweden

Publications

  1. ncbi Exploring the tumour environment: cancer-associated fibroblasts as targets in cancer therapy
    Patrick Micke
    Department of Genetics and Pathology, Uppsala University, S 751 85, Uppsala, Sweden
    Expert Opin Ther Targets 9:1217-33. 2005
  2. ncbi A fluid cover medium provides superior morphology and preserves RNA integrity in tissue sections for laser microdissection and pressure catapulting
    Patrick Micke
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    J Pathol 202:130-8. 2004
  3. ncbi Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues
    Kenneth Wester
    Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Lab Invest 83:889-99. 2003
  4. doi Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis, and tissue microarray validation
    Johan Botling
    Departments of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Clin Cancer Res 19:194-204. 2013
  5. pmc Microsatellite instability and mutations in BRAF and KRAS are significant predictors of disseminated disease in colon cancer
    Helgi Birgisson
    Department of Surgical Sciences, Colorectal Surgery, Uppsala University, 75185, Uppsala, Sweden
    BMC Cancer 15:125. 2015
  6. ncbi Various Antibody Clones of Napsin A, Thyroid Transcription Factor 1, and p40 and Comparisons With Cytokeratin 5 and p63 in Histopathologic Diagnostics of Non-Small Cell Lung Carcinoma
    Lena Tran
    Department of Pathology, Regional Laboratories Region Skåne Department of Clinical Sciences, Division of Oncology and Pathology, Lund University Department of Thoracic Surgery, Lund University and Skåne University Hospital Department of Oncology, Skane University Hospital, Lund Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Appl Immunohistochem Mol Morphol 24:648-659. 2016
  7. pmc Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity
    Markus Rasmussen
    Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Akademiska sjukhuset, SE 751 85 Uppsala, Sweden
    Genome Biol 12:R108. 2011
  8. doi Microsatellite instability and mutations in BRAF and KRAS are significant predictors of disseminated disease in colon cancer
    Helgi Birgisson
    Department of Surgical Sciences, Colorectal Surgery, Uppsala University, 75185, Uppsala, Sweden
    BMC Cancer 15:1144. 2015
  9. doi Gene copy number aberrations are associated with survival in histologic subgroups of non-small cell lung cancer
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    J Thorac Oncol 6:1833-40. 2011
  10. doi The Impact of the Fourth Edition of the WHO Classification of Lung Tumours on Histological Classification of Resected Pulmonary NSCCs
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    J Thorac Oncol 11:862-72. 2016

Collaborators

Detail Information

Publications35

  1. ncbi Exploring the tumour environment: cancer-associated fibroblasts as targets in cancer therapy
    Patrick Micke
    Department of Genetics and Pathology, Uppsala University, S 751 85, Uppsala, Sweden
    Expert Opin Ther Targets 9:1217-33. 2005
    ..It is likely that the continued characterisation of this interaction, and the molecular identification of key mediators, will provide insights into tumour biology and suggest novel therapeutic options...
  2. ncbi A fluid cover medium provides superior morphology and preserves RNA integrity in tissue sections for laser microdissection and pressure catapulting
    Patrick Micke
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    J Pathol 202:130-8. 2004
    ..This not only facilitates established procedures, but will also extend the application to tissues that require superior morphological resolution...
  3. ncbi Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues
    Kenneth Wester
    Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Lab Invest 83:889-99. 2003
    ..We conclude that ZBF is superior to NBF for analysis of DNA and protein expression. Fixation of tissues in ZBF may also be an alternative strategy to freeze storage of tissue specimens, eg, in future bio-banks...
  4. doi Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis, and tissue microarray validation
    Johan Botling
    Departments of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Clin Cancer Res 19:194-204. 2013
    ..So far, the value of these multigene signatures in clinical practice is unclear, and the biologic importance of individual genes is difficult to assess, as the published signatures virtually do not overlap...
  5. pmc Microsatellite instability and mutations in BRAF and KRAS are significant predictors of disseminated disease in colon cancer
    Helgi Birgisson
    Department of Surgical Sciences, Colorectal Surgery, Uppsala University, 75185, Uppsala, Sweden
    BMC Cancer 15:125. 2015
    ....
  6. ncbi Various Antibody Clones of Napsin A, Thyroid Transcription Factor 1, and p40 and Comparisons With Cytokeratin 5 and p63 in Histopathologic Diagnostics of Non-Small Cell Lung Carcinoma
    Lena Tran
    Department of Pathology, Regional Laboratories Region Skåne Department of Clinical Sciences, Division of Oncology and Pathology, Lund University Department of Thoracic Surgery, Lund University and Skåne University Hospital Department of Oncology, Skane University Hospital, Lund Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Appl Immunohistochem Mol Morphol 24:648-659. 2016
    ..There is no substantial difference between monoclonal and polyclonal p40 and between different NAPA clones, whereas there is a difference between the TTF-1 clones 8G7G3/1 and SPT24...
  7. pmc Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity
    Markus Rasmussen
    Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Akademiska sjukhuset, SE 751 85 Uppsala, Sweden
    Genome Biol 12:R108. 2011
    ..Analysis of cancer samples indicates that TAPS is well suited to investigate samples with aneuploidy and tumor heterogeneity, which is commonly found in many types of solid tumors...
  8. doi Microsatellite instability and mutations in BRAF and KRAS are significant predictors of disseminated disease in colon cancer
    Helgi Birgisson
    Department of Surgical Sciences, Colorectal Surgery, Uppsala University, 75185, Uppsala, Sweden
    BMC Cancer 15:1144. 2015
    ....
  9. doi Gene copy number aberrations are associated with survival in histologic subgroups of non-small cell lung cancer
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    J Thorac Oncol 6:1833-40. 2011
    ..To maximize the chance to identify clinically relevant events, we applied a strategy involving two prognostically extreme patient groups...
  10. doi The Impact of the Fourth Edition of the WHO Classification of Lung Tumours on Histological Classification of Resected Pulmonary NSCCs
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    J Thorac Oncol 11:862-72. 2016
    ....
  11. doi Aberrantly activated claudin 6 and 18.2 as potential therapy targets in non-small-cell lung cancer
    Patrick Micke
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Int J Cancer 135:2206-14. 2014
    ..8; p = 0.03] and multivariate COX regression model (HR: 1.9; p = 0.02). These findings encourage further clinical exploration of targeting ectopically activated CLDN expression as a valuable treatment concept in NSCLC. ..
  12. pmc Profiling cancer testis antigens in non-small-cell lung cancer
    Dijana Djureinovic
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    JCI Insight 1:e86837. 2016
    ..The detailed RNAseq catalog can guide biomarker studies and efforts to identify targets for immunotherapeutic strategies...
  13. pmc Inconsistent results in the analysis of ALK rearrangements in non-small cell lung cancer
    Johanna S M Mattsson
    Department of Immunology, Genetics and Pathology, Uppsala University, 751 85, Uppsala, Sweden
    BMC Cancer 16:603. 2016
    ..In order to unbiasedly analyze the diagnostic impact of such a screening strategy, we compared ALK IHC with ALK FISH in three large representative Swedish NSCLC cohorts incorporating clinical parameters and gene expression data...
  14. pmc 1p36 deletion is a marker for tumour dissemination in microsatellite stable stage II-III colon cancer
    Markus Mayrhofer
    Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Box 3056, Uppsala 750 03, Sweden
    BMC Cancer 14:872. 2014
    ..We investigated 115 selected stage II-IV primary colon cancers for associations between chromosomal alterations and tumour dissemination...
  15. doi The lung-specific proteome defined by integration of transcriptomics and antibody-based profiling
    Cecilia Lindskog
    Rudbeck Laboratory, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
    FASEB J 28:5184-96. 2014
    ..As biomarker candidates, these proteins may represent intriguing starting points for further exploration in health and disease...
  16. ncbi Patients with Non-small Cell Lung Cancer Analyzed for EGFR: Adherence to Guidelines, Prevalence and Outcome
    Martin Sandelin
    Department of Medical Sciences, Respiratory Medicine, Uppsala University, Uppsala, Sweden
    Anticancer Res 35:3979-85. 2015
    ..In the present study, we describe the prevalence of EGFR mutations and the adherence to testing and treatment guidelines in a population-based Swedish NSCLC cohort...
  17. doi Prognostic impact of COX-2 in non-small cell lung cancer: a comprehensive compartment-specific evaluation of tumor and stromal cell expression
    Johanna S M Mattsson
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Cancer Lett 356:837-45. 2015
    ....
  18. pmc Data-driven unbiased curation of the TP53 tumor suppressor gene mutation database and validation by ultradeep sequencing of human tumors
    Karolina Edlund
    Department of Immunology, and SciLifeLab Uppsala, Uppsala University, SE 751 85 Uppsala, Sweden
    Proc Natl Acad Sci U S A 109:9551-6. 2012
    ..Thus, by combining statistical and experimental analyses, we provide a curated mutation database for TP53 mutations and a framework for mutation database analysis...
  19. doi CD99 is a novel prognostic stromal marker in non-small cell lung cancer
    Karolina Edlund
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Int J Cancer 131:2264-73. 2012
    ..The results support the concept that stromal properties have an important impact on tumour progression...
  20. doi HaloPlex Targeted Resequencing for Mutation Detection in Clinical Formalin-Fixed, Paraffin-Embedded Tumor Samples
    Lotte N J Moens
    Department of Immunology Genetics and Pathology, Uppsala University, Science for Life Laboratory, Uppsala, Sweden
    J Mol Diagn 17:729-39. 2015
    ..We believe that the HaloPlex method presented here will be broadly applicable as a tool for somatic mutation detection in clinical cancer settings. ..
  21. doi Consistent mutation status within histologically heterogeneous lung cancer lesions
    Johanna Sofia Margareta Mattsson
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
    Histopathology 61:744-8. 2012
    ..The aim of this study was to characterize the presence of EGFR and KRAS mutations in histologically different areas of primary NSCLC lesions...
  22. doi Immunohistochemistry-based prognostic biomarkers in NSCLC: novel findings on the road to clinical use?
    Cecilia Lindskog
    Department of Immunology Genetics and Pathology, Science for Life Laboratory, Uppsala University, 751 85 Uppsala, Sweden
    Expert Rev Mol Diagn 15:471-90. 2015
    ..Raised quality standards for study design and antibody validation, and integration of preclinical findings with clinical needs are clearly warranted...
  23. pmc KRAS analysis in colorectal carcinoma: analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice
    Magnus Sundstrom
    Molecular Pathology Unit, Department of Genetics and Pathology, Uppsala University, SE 751 85 Uppsala, Sweden
    BMC Cancer 10:660. 2010
    ..Several procedures to detect KRAS mutations have been developed. However, the analytical sensitivity and specificity of these assays on routine clinical samples are not yet fully characterised...
  24. doi Fresh frozen tissue: RNA extraction and quality control
    Johan Botling
    Rudbeck Laboratory, Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Methods Mol Biol 675:405-13. 2011
    ..To this end, we here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis...
  25. doi Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue
    Johan Botling
    Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Diagn Mol Pathol 18:44-52. 2009
    ..In conclusion, degradation of RNA in frozen tissue occurs first after several minutes of thawing. Already minimal decrease in RNA quality may result in significant changes in gene expression patterns in clinical tissue samples...
  26. pmc The protein kinase LKB1 negatively regulates bone morphogenetic protein receptor signaling
    Erna Raja
    Ludwig Institute for Cancer Research, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
    Oncotarget 7:1120-43. 2016
    ..This new mechanism of BMP receptor regulation by LKB1 has ramifications in physiological organogenesis and disease. ..
  27. doi A novel strategy based on histological protein profiling in-silico for identifying potential biomarkers in urinary bladder cancer
    M Ulrika Segersten
    Department of Surgical Sciences Urology, Uppsala University Hospital, Uppsala, Sweden
    BJU Int 104:1780-5. 2009
    ..To screen a publicly available immunohistochemistry (IHC) based web-atlas, to identify key proteins in bladder cancer that might serve as potential biomarkers...
  28. pmc Quantification of normal cell fraction and copy number neutral LOH in clinical lung cancer samples using SNP array data
    Hanna Göransson
    Department of Medical Sciences, Uppsala University, Uppsala, Sweden
    PLoS ONE 4:e6057. 2009
    ..Since tumor tissue invariably contains genetically normal stromal cells, this may lead to a failure to detect aberrations in the tumor cells...
  29. doi Biobanking of fresh frozen tissue from clinical surgical specimens: transport logistics, sample selection, and histologic characterization
    Johan Botling
    Department of Genetics and Pathology, University Hospital, Uppsala, Sweden
    Methods Mol Biol 675:299-306. 2011
    ..Also, knowledge of the histomorphology of the frozen tissue sample - tumor cell content, stromal components, and presence of necrosis - is pivotal before entering a biobank case into costly molecular profiling studies...
  30. ncbi Tumour-stroma interaction: cancer-associated fibroblasts as novel targets in anti-cancer therapy?
    Patrick Micke
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    Lung Cancer 45:S163-75. 2004
    ..Finally, a laser-capture- and microarray-based approach is presented, which comprehensively characterises carcinoma-associated fibroblasts in their in vivo environment for the identification of potential targets for anti-cancer therapy...
  31. ncbi Laser-assisted cell microdissection using the PALM system
    Patrick Micke
    Cancer Center Karolinska, Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden
    Methods Mol Biol 293:151-66. 2005
    ..The described procedures emphasize preparation of tissue, laser microdissection including catapulting of cells, and extraction of RNA and DNA. Downstream experiments for validation are also shown...
  32. ncbi Dynamic changes in the expression of DEP-1 and other PDGF receptor-antagonizing PTPs during onset and termination of neointima formation
    Kai Kappert
    Cancer Centrum Karolinska, Department of Oncology Pathology, Karolinska Institutet, 17176 Stockholm, Sweden
    FASEB J 21:523-34. 2007
    ..In more general terms, the observations indicate transcriptional regulation of PTPs as an important mechanism for controlling onset and termination of RTK-dependent tissue remodeling...
  33. ncbi In situ identification of genes regulated specifically in fibroblasts of human basal cell carcinoma
    Patrick Micke
    Department of Oncology Pathology, Cancer Centrum Karolinska, Karolinska Institutet, Stockholm, Sweden
    J Invest Dermatol 127:1516-23. 2007
    ..The results suggest a specific expression profile of CAFs in BCC possibly accounting for disease-specific pathological roles...
  34. ncbi In vitro characterization of the cytokine profile of the epithelial cell rests of Malassez
    Mitsuhiro Ohshima
    Department of Biochemistry, Nihon University School of Dentistry, 1 8 13 Kanda Surugadai, Chiyoda ku, Tokyo, Japan
    J Periodontol 79:912-9. 2008
    ..The aim of this study was to establish primary cell cultures of human ERM, characterize the cytokine profile, and compare it to other periodontal cell entities...
  35. ncbi Downregulation of bradykinin B2 receptor in human fibroblasts during prolonged agonist exposure
    Andree Blaukat
    Institute of Pharmacology, University of Heidelberg, Germany
    Am J Physiol Heart Circ Physiol 284:H1909-16. 2003
    ..e., by attenuation of de novo synthesis and by reduction of receptor stability...