Jochen M Schwenk

Summary

Affiliation: Royal Institute of Technology
Country: Sweden

Publications

  1. doi request reprint Antibody suspension bead arrays within serum proteomics
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteome Res 7:3168-79. 2008
  2. doi request reprint Comparative protein profiling of serum and plasma using an antibody suspension bead array approach
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    Proteomics 10:532-40. 2010
  3. pmc Autoantibody profiling in multiple sclerosis using arrays of human protein fragments
    Burcu Ayoglu
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 12:2657-72. 2013
  4. doi request reprint Classification of protein profiles from antibody microarrays using heat and detergent treatment
    Anna Häggmark
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    N Biotechnol 29:564-70. 2012
  5. doi request reprint Systematic antibody and antigen-based proteomic profiling with microarrays
    Burcu Ayoglu
    Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Expert Rev Mol Diagn 11:219-34. 2011
  6. doi request reprint A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Microbiol Methods 82:11-8. 2010
  7. doi request reprint Validation of serum protein profiles by a dual antibody array approach
    Rebecca Rimini
    Department of Proteomics, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteomics 73:252-66. 2009
  8. doi request reprint Antibody-based profiling of cerebrospinal fluid within multiple sclerosis
    Anna Häggmark
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Proteomics 13:2256-67. 2013
  9. doi request reprint Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
    Ulrika Qundos
    Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    J Proteomics 95:46-54. 2013
  10. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011

Collaborators

Detail Information

Publications52

  1. doi request reprint Antibody suspension bead arrays within serum proteomics
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteome Res 7:3168-79. 2008
    ..The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins...
  2. doi request reprint Comparative protein profiling of serum and plasma using an antibody suspension bead array approach
    Jochen M Schwenk
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    Proteomics 10:532-40. 2010
    ....
  3. pmc Autoantibody profiling in multiple sclerosis using arrays of human protein fragments
    Burcu Ayoglu
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Mol Cell Proteomics 12:2657-72. 2013
    ....
  4. doi request reprint Classification of protein profiles from antibody microarrays using heat and detergent treatment
    Anna Häggmark
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    N Biotechnol 29:564-70. 2012
    ..The experimental scheme presented here can be used to systematically categorize antibodies and thereby combine antibodies with similar properties into targeted arrays for analysis of plasma and serum...
  5. doi request reprint Systematic antibody and antigen-based proteomic profiling with microarrays
    Burcu Ayoglu
    Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Expert Rev Mol Diagn 11:219-34. 2011
    ....
  6. doi request reprint A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    J Microbiol Methods 82:11-8. 2010
    ....
  7. doi request reprint Validation of serum protein profiles by a dual antibody array approach
    Rebecca Rimini
    Department of Proteomics, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    J Proteomics 73:252-66. 2009
    ....
  8. doi request reprint Antibody-based profiling of cerebrospinal fluid within multiple sclerosis
    Anna Häggmark
    SciLifeLab Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Proteomics 13:2256-67. 2013
    ..SERPINA3 instead was detected at higher levels in all MS patients compared to controls. The developed assay procedure now offers new possibilities for broad-scale protein profiling of CSF within neurological disorders. ..
  9. doi request reprint Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
    Ulrika Qundos
    Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    J Proteomics 95:46-54. 2013
    ....
  10. pmc Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
    Barbara Hjelm
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, 10691 Stockholm, Sweden
    Protein Sci 20:1824-35. 2011
    ....
  11. doi request reprint Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment
    Maja Neiman
    Royal Institute of Technology, Solna, Sweden
    J Proteome Res 10:4925-34. 2011
    ....
  12. pmc Affinity proteomics discovers decreased levels of AMFR in plasma from Osteoporosis patients
    Ulrika Qundos
    Affinity Proteomics, SciLifeLab, KTH Royal Institute of Technology, Solna, Sweden
    Proteomics Clin Appl 10:681-90. 2016
    ..In the presented study, we investigated blood plasma within osteoporosis to discovery differential protein profiles and to propose novel biomarkers candidates for subsequent studies...
  13. doi request reprint Identification of a Novel Autoimmune Peptide Epitope of Prostein in Prostate Cancer
    Elisa Pin
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, 171 65 Solna, Sweden
    J Proteome Res 16:204-216. 2017
    ..This study provides useful information when searching for novel biomarkers or drug targets that are guided by the reactivity of the immune system against autoantigens...
  14. doi request reprint Affinity proteomic profiling of plasma, cerebrospinal fluid, and brain tissue within multiple sclerosis
    Sanna Byström
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm 171 21, Sweden
    J Proteome Res 13:4607-19. 2014
    ..The affinity proteomic translation of profiles discovered by profiling human body fluids and tissue provides a powerful strategy to suggest additional candidates to studies of neurological disorders. ..
  15. doi request reprint Validation of affinity reagents using antigen microarrays
    Ronald Sjöberg
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Box 1031, SE 171 21 Solna, Sweden
    N Biotechnol 29:555-63. 2012
    ..The presented study illustrates the feasibility of using antigen microarrays for integrative, high-throughput validation of various types of binders and antigens...
  16. pmc Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays
    Jochen M Schwenk
    Science for Life Laboratory, Royal Institute of Technology KTH, Stockholm, Sweden
    Mol Cell Proteomics 9:2497-507. 2010
    ..The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format...
  17. doi request reprint Exploration of high-density protein microarrays for antibody validation and autoimmunity profiling
    Ronald Sjöberg
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    N Biotechnol 33:582-92. 2016
    ....
  18. ncbi request reprint Autoantibody targets in vaccine-associated narcolepsy
    Anna Häggmark-Månberg
    a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Autoimmunity 49:421-433. 2016
    ..These findings could become useful elements of further clinical assays that aim towards a better phenotypic understanding of narcolepsy and its triggers...
  19. pmc Elevated levels of FN1 and CCL2 in bronchoalveolar lavage fluid from sarcoidosis patients
    Carl Hamsten
    Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and University Hospital, Stockholm, Sweden
    Respir Res 17:69. 2016
    ..Several gene associations have previously been described, but established and clinically useful biomarkers are still absent. This study aimed to find proteins in bronchoalveolar lavage (BAL) fluid that can be associated with the disease...
  20. pmc Immunocapture strategies in translational proteomics
    Claudia Fredolini
    a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Solna, Sweden
    Expert Rev Proteomics 13:83-98. 2016
    ..Discussed are some of their advantages, pitfalls and opportunities for the next phase in clinical and translational proteomics. ..
  21. pmc Selectivity analysis of single binder assays used in plasma protein profiling
    Maja Neiman
    Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Solna, Sweden
    Proteomics 13:3406-10. 2013
    ..The presented approach will serve as an important tool for resolving differential protein profiles from antibody arrays within plasma biomarker discoveries. ..
  22. doi request reprint Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP)
    Linn Fagerberg
    Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden
    J Proteome Res 12:2439-48. 2013
    ..The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas ( www.proteinatlas.org )...
  23. pmc Multiplex screening of surface proteins from Mycoplasma mycoides subsp. mycoides small colony for an antigen cocktail enzyme-linked immunosorbent assay
    Maja Neiman
    Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Clin Vaccine Immunol 16:1665-74. 2009
    ..This set of proteins and technologies therefore offers a powerful combination to drive and further improve serological assays toward reliable, simple, and cost-effective diagnosis of CBPP...
  24. doi request reprint Assessment of antibody specificity using suspension bead arrays
    Jochen M Schwenk
    SciLifeLab Stockholm, KTH Royal Institute of Technology, Tomtebodav, Sweden
    Methods Mol Biol 785:183-9. 2011
    ..While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility...
  25. doi request reprint Proteomics. Tissue-based map of the human proteome
    Mathias Uhlen
    Science for Life Laboratory, KTH Royal Institute of Technology, SE 171 21 Stockholm, Sweden Department of Proteomics, KTH Royal Institute of Technology, SE 106 91 Stockholm, Sweden Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK 2970 Hørsholm, Denmark
    Science 347:1260419. 2015
    ..All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body. ..
  26. doi request reprint Highly multiplexed antibody suspension bead arrays for plasma protein profiling
    Kimi Drobin
    Science for Life Laboratory Stockholm, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Methods Mol Biol 1023:137-45. 2013
    ..Presently, this workflow enables the profiling of 384 samples for up to 384 proteins per assay...
  27. ncbi request reprint Determination of binding specificities in highly multiplexed bead-based assays for antibody proteomics
    Jochen M Schwenk
    Department of Proteomics, Royal Institute of Technology KTH, SE 10691 Stockholm, Sweden
    Mol Cell Proteomics 6:125-32. 2007
    ..This high throughput analysis will potentially have great impact on the possibility for the utilization of different antibody proteomics approaches where the quality assessment of antibodies is of the utmost importance...
  28. pmc Anoctamin 2 identified as an autoimmune target in multiple sclerosis
    Burcu Ayoglu
    Affinity Proteomics, Science for Life Laboratory, School of Biotechnology, Royal Institute of Technology KTH, SE 17165 Stockholm, Sweden
    Proc Natl Acad Sci U S A 113:2188-93. 2016
    ..The findings presented here demonstrate that an ANO2 autoimmune subphenotype may exist in MS and lay the groundwork for further studies focusing on the pathogenic role of ANO2 autoantibodies in MS. ..
  29. doi request reprint Heat differentiated complement factor profiling
    Carl Hamsten
    Clinical Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and University Hospital, Stockholm, Sweden Center for Inflammatory Diseases, Karolinska Institutet, Stockholm, Sweden
    J Proteomics 126:155-62. 2015
    ..Our study demonstrates the potential of using multiplexed single binder assays for screening of complement components that open possibilities to expand such analysis to other forms of deficiencies. ..
  30. doi request reprint Parallel barcoding of antibodies for DNA-assisted proteomics
    Mahya Dezfouli
    Science for Life Laboratory, Division of Gene Technology, School of Biotechnology, Royal Institute of Technology KTH, Solna, Sweden
    Proteomics 14:2432-6. 2014
    ..Conclusively, human plasma samples were analyzed to indicate the functionality of barcoded antibodies in intended proteomics applications...
  31. doi request reprint Antibody suspension bead arrays
    Jochen M Schwenk
    Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, 10691, Stockholm, Sweden
    Methods Mol Biol 723:29-36. 2011
    ..Presently, this workflow enables the profiling of 384 clinical samples for up to 100 proteins per assay...
  32. pmc Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type
    Carl Hamsten
    Department of Proteomics, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 8:2544-54. 2009
    ..In conclusion, a toolbox with recombinant proteins and a flexible suspension array assay that allows multiplex analysis of humoral immune responses to M. mycoides SC has been created...
  33. pmc Multiplexed protein profiling by sequential affinity capture
    Burcu Ayoglu
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Solna, Sweden
    Proteomics 16:1251-6. 2016
    ..Hence, the described concept enhances the possibilities for antibody array assays to be utilized for protein profiling in body fluids and beyond...
  34. pmc CSF profiling of the human brain enriched proteome reveals associations of neuromodulin and neurogranin to Alzheimer's disease
    Julia Remnestål
    Affinity Proteomics, SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
    Proteomics Clin Appl 10:1242-1253. 2016
    ....
  35. doi request reprint Proteomic profiling reveals autoimmune targets in sarcoidosis
    Anna Häggmark
    1 SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Am J Respir Crit Care Med 191:574-83. 2015
    ..There is a need to further characterize the antibody repertoire in relation to sarcoidosis and potentially related autoantigens...
  36. pmc Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
    Linn Fagerberg
    Science for Life Laboratory, KTH Royal Institute of Technology, SE 171 21 Stockholm, Sweden
    Mol Cell Proteomics 13:397-406. 2014
    ..The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body. ..
  37. doi request reprint Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies
    Johan Rockberg
    School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Mol Oncol 3:238-47. 2009
    ..The work describes a new strategy to develop antibodies directed to non-overlapping epitopes and shows a path of pursuit to explore the epitope space of a target protein...
  38. doi request reprint Magnetic bead-based detection of autoimmune responses using protein microarrays
    Jesper Gantelius
    Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
    N Biotechnol 26:269-76. 2009
    ..We propose from our findings that the magnetic bead-based detection option provides a simplified and cost effective readout method for protein microarrays...
  39. ncbi request reprint PDGFB, a new candidate plasma biomarker for venous thromboembolism: results from the VEREMA affinity proteomics study
    Maria Bruzelius
    Coagulation Unit, Hematology Centre, Karolinska University Hospital, Stockholm, Sweden
    Blood 128:e59-e66. 2016
    ....
  40. ncbi request reprint Multidimensional Normalization to Minimize Plate Effects of Suspension Bead Array Data
    Mun Gwan Hong
    Affinity Proteomics, Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Solna 171 65, Sweden
    J Proteome Res 15:3473-3480. 2016
    ..Our normalization approaches, which are available as R package MDimNormn, could also be useful in studies using other types of high-throughput assay data...
  41. pmc Elevated levels of circulating CDH5 and FABP1 in association with human drug-induced liver injury
    Maria Mikus
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Liver Int . 2016
    ..To identify novel biomarker candidates associated to DILI, we utilized an affinity proteomics strategy, where antibody suspension bead arrays were applied to profile plasma and serum samples from human DILI cases and controls...
  42. doi request reprint Neuroproteomic profiling of human body fluids
    Anna Häggmark
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Proteomics Clin Appl 10:485-502. 2016
    ..Here, we provide an overview of how such technologies have been applied to study neurological disease and we also discuss some important considerations concerning discovery of disease-associated profiles...
  43. doi request reprint Antigen arrays for profiling autoantibody repertoires
    Burcu Ayoglu
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
    Bioanalysis 8:1105-26. 2016
    ..Together with recent studies utilizing antigen arrays, we give an overview on how the different types of antigen arrays have and will continue to deliver novel insights into autoimmune diseases among several others. ..
  44. pmc Circulating carnosine dipeptidase 1 associates with weight loss and poor prognosis in gastrointestinal cancer
    Peter Arner
    Department of Medicine H7, Karolinska Institutet, Karolinska University Hospital, Huddinge, 141 86, Stockholm, Sweden
    PLoS ONE 10:e0123566. 2015
    ..Although the mechanisms promoting this condition are not known, several circulating proteins have been proposed to contribute. We analyzed the plasma proteome in cancer subjects in order to identify factors associated with cachexia...
  45. doi request reprint Analysis of autoantibody profiles in osteoarthritis using comprehensive protein array concepts
    Frauke Henjes
    Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, Stockholm SE 171 21, Sweden
    J Proteome Res 13:5218-29. 2014
    ....
  46. pmc Affinity proteomics within rare diseases: a BIO-NMD study for blood biomarkers of muscular dystrophies
    Burcu Ayoglu
    Affinity Proteomics, SciLifeLab, School of Biotechnology KTH Royal Institute of Technology, Stockholm, Sweden
    EMBO Mol Med 6:918-36. 2014
    ..Using age-matched sub-cohorts, 9 protein profiles correlating with disease progression and severity were identified, which hold promise for the development of new clinical tools for management of dystrophinopathies. ..
  47. doi request reprint Magnetic bead assisted labeling of antibodies at nanogram scale
    Mahya Dezfouli
    Science for Life Laboratory, Division of Gene Technology, School of Biotechnology, Royal Institute of Technology KTH, Solna, Sweden
    Proteomics 14:14-8. 2014
    ..With the support of magnetic microspheres, automated labeling of antibodies in parallel using biotin or fluorescent dyes was achieved. ..
  48. doi request reprint Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples
    Cecilia Eriksson
    AlbaNova University Center, Department of Biotechnology, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Biotechnol Appl Biochem 56:49-57. 2010
    ..A sample-processing platform like this should be suitable for a number of proteomics technologies; its flexibility in buffer composition allows for different types of downstream applications...
  49. ncbi request reprint Cell microarrays: an emerging technology for the characterization of antibodies
    Jochen M Schwenk
    NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
    Biotechniques . 2002
    ..This new method is applicable for rapidly screening cell surface-specific antibodies with respect to selectivity and cross-reactivity...
  50. ncbi request reprint Protein microarrays for antibody profiling: specificity and affinity determination on a chip
    Oliver Poetz
    Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
    Proteomics 5:2402-11. 2005
    ..The new microarray based approach has an extraordinary potential to speed up the screening process for the generation of recombinant antibodies with pre-defined selection criteria, since it is intrinsically a high-throughput technology...
  51. ncbi request reprint Protein microarrays: catching the proteome
    Oliver Poetz
    NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr 55, 72770 Reutlingen, Germany
    Mech Ageing Dev 126:161-70. 2005
    ..We will focus on the latest fields of application for the simultaneous determination of a variety of parameters from a minute amount of sample. Future challenges of this cutting-edge technology will be discussed...
  52. ncbi request reprint Protein microarrays: promising tools for proteomic research
    Markus F Templin
    NMI Natural and Medical Sciences Institute, University of Tubingen, Markwiesenstrasse 55, D 72770 Reutlingen, Germany
    Proteomics 3:2155-66. 2003
    ..Microarray technology is an emerging technology used in global analytical approaches and has a considerable impact on proteomic research...