Gunnar Sturfelt

Summary

Affiliation: Lund University
Country: Sweden

Publications

  1. pmc Streptococcus pneumoniae phosphoglycerate kinase is a novel complement inhibitor affecting the membrane attack complex formation
    Anna M Blom
    From the Section of Medical Protein Chemistry, Department of Laboratory Medicine Malmö, Lund University, 20502 Malmo, Sweden
    J Biol Chem 289:32499-511. 2014
  2. pmc C4b-binding protein binds to necrotic cells and DNA, limiting DNA release and inhibiting complement activation
    Leendert A Trouw
    Department of Laboratory Medicine, University Hospital Malmo, Lund University, S 205 02 Malmo, Sweden
    J Exp Med 201:1937-48. 2005
  3. pmc Annexin-II, DNA, and histones serve as factor H ligands on the surface of apoptotic cells
    Jonatan Leffler
    Department of Laboratory Medicine, Wallenberg Laboratory, University Hospital Malmo, Lund University, S 205 02 Malmo, Sweden
    J Biol Chem 285:3766-76. 2010
  4. pmc C4b-binding protein is present in affected areas of myocardial infarction during the acute inflammatory phase and covers a larger area than C3
    Leendert A Trouw
    Department of Laboratory Medicine, Medical Protein Chemistry, Lund University, Malmo, Sweden
    PLoS ONE 3:e2886. 2008
  5. pmc The C-type lectin of the aggrecan G3 domain activates complement
    Camilla Melin Fürst
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, Lund University, Malmo, Sweden
    PLoS ONE 8:e61407. 2013
  6. pmc Functional analyses of complement convertases using C3 and C5-depleted sera
    Marcin Okroj
    Department of Laboratory Medicine, Lund University, The Wallenberg Laboratory, Malmo, Sweden
    PLoS ONE 7:e47245. 2012
  7. pmc Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia
    Sven Malm
    Section of Medical Protein Chemistry, Department of Laboratory Medicine, Skane University Hospital, Lund University, Malmo, Sweden
    PLoS ONE 7:e34852. 2012
  8. doi The novel complement inhibitor human CUB and Sushi multiple domains 1 (CSMD1) protein promotes factor I-mediated degradation of C4b and C3b and inhibits the membrane attack complex assembly
    Astrid Escudero-Esparza
    1Department of Laboratory Medicine Malmö, Section of Medical Protein Chemistry, Skane University Hospital, The Wallenberg Laboratory, Inga Marie Nilssons gata 53, 20502 Malmo, Sweden
    FASEB J 27:5083-93. 2013
  9. doi Sushi domain-containing protein 4 (SUSD4) inhibits complement by disrupting the formation of the classical C3 convertase
    Emelie Holmquist
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, Lund University, Malmo, Sweden
    FASEB J 27:2355-66. 2013
  10. pmc Serum COMP-C3b complexes in rheumatic diseases and relation to anti-TNF-α treatment
    Kaisa E Happonen
    Department of Laboratory Medicine Malmö, Section of Medical Protein Chemistry, Lund University, Wallenberg Laboratory Floor 4, SE 205 02 Malmo, Sweden
    Arthritis Res Ther 14:R15. 2012

Detail Information

Publications81

  1. pmc Streptococcus pneumoniae phosphoglycerate kinase is a novel complement inhibitor affecting the membrane attack complex formation
    Anna M Blom
    From the Section of Medical Protein Chemistry, Department of Laboratory Medicine Malmö, Lund University, 20502 Malmo, Sweden
    J Biol Chem 289:32499-511. 2014
    ..In conclusion, our data demonstrate for the first time to our knowledge a novel pneumococcal inhibitor of the terminal complement cascade aiding complement evasion by this important pathogen. ..
  2. pmc C4b-binding protein binds to necrotic cells and DNA, limiting DNA release and inhibiting complement activation
    Leendert A Trouw
    Department of Laboratory Medicine, University Hospital Malmo, Lund University, S 205 02 Malmo, Sweden
    J Exp Med 201:1937-48. 2005
    ..This study describes a novel mechanism in which C4BP limits the inflammatory potential of necrotic cells...
  3. pmc Annexin-II, DNA, and histones serve as factor H ligands on the surface of apoptotic cells
    Jonatan Leffler
    Department of Laboratory Medicine, Wallenberg Laboratory, University Hospital Malmo, Lund University, S 205 02 Malmo, Sweden
    J Biol Chem 285:3766-76. 2010
    ....
  4. pmc C4b-binding protein is present in affected areas of myocardial infarction during the acute inflammatory phase and covers a larger area than C3
    Leendert A Trouw
    Department of Laboratory Medicine, Medical Protein Chemistry, Lund University, Malmo, Sweden
    PLoS ONE 3:e2886. 2008
    ..In the present study we investigated whether fluid-phase complement inhibitor C4b-binding protein (C4BP) would also bind to the infarcted heart tissue...
  5. pmc The C-type lectin of the aggrecan G3 domain activates complement
    Camilla Melin Fürst
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, Lund University, Malmo, Sweden
    PLoS ONE 8:e61407. 2013
    ..This was most apparent at the level of deposition of terminal complement components. Taken together our observations indicate aggrecan CLD as one factor involved in the sustained inflammation of the joint...
  6. pmc Functional analyses of complement convertases using C3 and C5-depleted sera
    Marcin Okroj
    Department of Laboratory Medicine, Lund University, The Wallenberg Laboratory, Malmo, Sweden
    PLoS ONE 7:e47245. 2012
    ..As an example of practical utility of the assay, we performed studies on thioredoxin-1 in order to clarify the mechanism of its influence on complement convertases...
  7. pmc Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia
    Sven Malm
    Section of Medical Protein Chemistry, Department of Laboratory Medicine, Skane University Hospital, Lund University, Malmo, Sweden
    PLoS ONE 7:e34852. 2012
    ..We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases...
  8. doi The novel complement inhibitor human CUB and Sushi multiple domains 1 (CSMD1) protein promotes factor I-mediated degradation of C4b and C3b and inhibits the membrane attack complex assembly
    Astrid Escudero-Esparza
    1Department of Laboratory Medicine Malmö, Section of Medical Protein Chemistry, Skane University Hospital, The Wallenberg Laboratory, Inga Marie Nilssons gata 53, 20502 Malmo, Sweden
    FASEB J 27:5083-93. 2013
    ..Our results revealed a novel complement inhibitor for the classical and lectin pathways...
  9. doi Sushi domain-containing protein 4 (SUSD4) inhibits complement by disrupting the formation of the classical C3 convertase
    Emelie Holmquist
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, Lund University, Malmo, Sweden
    FASEB J 27:2355-66. 2013
    ..Overall, our results show that SUSD4 is a novel complement inhibitor with restricted expression...
  10. pmc Serum COMP-C3b complexes in rheumatic diseases and relation to anti-TNF-α treatment
    Kaisa E Happonen
    Department of Laboratory Medicine Malmö, Section of Medical Protein Chemistry, Lund University, Wallenberg Laboratory Floor 4, SE 205 02 Malmo, Sweden
    Arthritis Res Ther 14:R15. 2012
    ..We now set out to elaborate on the information provided by this marker in a variety of diseases and larger patient cohorts...
  11. doi Complement evasion strategies of pathogens-acquisition of inhibitors and beyond
    Anna M Blom
    Lund University, Deptartment of Laboratory Medicine, University Hospital Malmo, Sweden
    Mol Immunol 46:2808-17. 2009
    ....
  12. doi C4b-binding protein (C4BP) inhibits development of experimental arthritis in mice
    A M Blom
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, Lund University, University Hospital Malmö entrance 46, The Wallenberg Laboratory floor 4, S 205 02 Malmo, Sweden
    Ann Rheum Dis 68:136-42. 2009
    ..To assess the human complement inhibitor C4b-binding protein (C4BP) for treatment of arthritis...
  13. ncbi Contribution of interactions between complement inhibitor C4b-binding protein and pathogens to their ability to establish infection with particular emphasis on Neisseria gonorrhoeae
    Anna M Blom
    Lund University, Department of Laboratory Medicine, Division of Medical Protein Chemistry, University Hospital Malmö entrance 46, The Wallenberg Laboratory floor 4, S 205 02 Malmo, Sweden
    Vaccine 26:I49-55. 2008
    ....
  14. doi Increased serum levels of S100A8/A9 and S100A12 are associated with cardiovascular disease in patients with inactive systemic lupus erythematosus
    Helena Tydén
    Department of Clinical Sciences, Division of Rheumatology, Lund University, SE 221 85 Lund, Sweden
    Rheumatology (Oxford) 52:2048-55. 2013
    ..The aim of the present study was to investigate whether there is an association between CVD and serum levels of the proinflammatory proteins S100A8/A9 and S100A12 in SLE...
  15. ncbi A novel interaction between type IV pili of Neisseria gonorrhoeae and the human complement regulator C4B-binding protein
    A M Blom
    Department of Clinical Chemistry, The Wallenberg Laboratory, Floor 6, Lund University, University Hospital Malmo, S 205 02 Malmo, Sweden
    J Immunol 166:6764-70. 2001
    ..Accordingly, high concentrations of C4BP were required to inhibit binding of N. gonorrhoeae to Chang conjunctiva cells, and no inhibition of binding was observed with cervical epithelial cells...
  16. pmc Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus
    Christian Lood
    Department of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University, Solvegatan 23, 223 62 Lund, Sweden
    Arthritis Res Ther 13:R60. 2011
    ..In this study we investigated if aberrant cell surface S100A8/A9 could be seen in SLE and if plasmacytoid dendritic cells (pDCs) could synthesize S100A8/A9...
  17. ncbi A cluster of positively charged amino acids in the alpha-chain of C4b-binding protein (C4BP) is pivotal for the regulation of the complement system and the interaction with bacteria
    A M Blom
    Department of Clinical Chemistry, Lund University, Wallenberg Laboratory, University Hospital Malmo, S 205 02, Malmo, Sweden
    Scand J Clin Lab Invest Suppl 233:37-49. 2000
    ..We suggest that binding between C4b and C4BP is governed mostly by electrostatic interactions, while additional non-covalent forces cause tight binding of C4BP to streptococcal M proteins...
  18. doi Design of recombinant antibody microarrays for urinary proteomics
    Malin Kristensson
    Department of Immunotechnology, Lund University, Lund, Sweden
    Proteomics Clin Appl 6:291-6. 2012
    ..In order to decipher complex proteomes, high demands will, however, be placed upon the methodology applied. The purpose of this study was to develop a recombinant antibody microarray platform for urinary proteomics...
  19. pmc The assessment of serum-mediated phagocytosis of necrotic material by polymorphonuclear leukocytes to diagnose and predict the clinical features of systemic lupus erythematosus: an observational longitudinal study
    Michele Compagno
    Department of Clinical Sciences, Section of Rheumatology, Lund University, Lund, Sweden
    Arthritis Res Ther 18:44. 2016
    ..In this study, we aimed to investigate the diagnostic accuracy of the assay and the relationship with clinical manifestations and disease activity in systemic lupus erythematosus (SLE)...
  20. doi Platelet transcriptional profile and protein expression in patients with systemic lupus erythematosus: up-regulation of the type I interferon system is strongly associated with vascular disease
    Christian Lood
    Department of Laboratory Medicine, Section of Microbiology, Immunology and Glycobiology, Lund University Hospital, Solvegatan 23, Lund, Sweden
    Blood 116:1951-7. 2010
    ..This could affect platelet activation and contribute to development of vascular disease in SLE. In addition, platelets with type I IFN signature could be a novel marker for vascular disease in SLE...
  21. pmc Plasma concentrations of Gas6 and sAxl correlate with disease activity in systemic lupus erythematosus
    Carl Ekman
    Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, Skane University Hospital, Malmo, Sweden
    Rheumatology (Oxford) 50:1064-9. 2011
    ..We have recently developed immunological methods to quantify the vitamin-K-dependent protein Gas6 and its soluble receptor sAxl in human plasma, which we have used to investigate the role of Gas6 and soluble Axl in SLE...
  22. pmc Increased plasma levels of the soluble Mer tyrosine kinase receptor in systemic lupus erythematosus relate to disease activity and nephritis
    Jun Wu
    Department of Laboratory Medicine, Section of Clinical Chemistry, Lund University, Wallenberg Laboratory, Skane University Hospital, Södra Förstadsgatan 101, Malmo, SE 205 02, Sweden
    Arthritis Res Ther 13:R62. 2011
    ..The objective of this study was to elucidate whether the plasma levels of soluble Mer (sMer) and Tyro3 (sTyro3) were increased in systemic lupus erythematosis (SLE), rheumatoid arthritis (RA), or critical limb ischemia (CLI)...
  23. ncbi Localization of a hydrophobic binding site for anticoagulant protein S on the beta -chain of complement regulator C4b-binding protein
    J H Webb
    Division of Clinical Chemistry, Department of Laboratory Medicine, Lund University, , , Sweden
    J Biol Chem 276:4330-7. 2001
    ..In addition, Lys(41) and Lys(42) contribute slightly to the interaction. Our results further confirm that surface hydrophobicity analysis may be used to identify ligand recognition sites...
  24. ncbi The complement regulator C4b-binding protein analyzed by molecular modeling, bioinformatics and computer-aided experimental design
    B O Villoutreix
    Lund University, The Wallenberg Laboratory, Department of Clinical Chemistry, University Hospital, Malmo, Sweden
    Immunopharmacology 42:121-34. 1999
    ..We also propose that the first CCP of the C4BP beta-chain displays a key hydrophobic surface of major importance for the interaction with the coagulation cofactor protein S...
  25. ncbi Positively charged amino acids at the interface between alpha-chain CCP1 and CCP2 of C4BP are required for regulation of the classical C3-convertase
    A M Blom
    Department of Clinical Chemistry, The Wallenberg Laboratory, Lund University, University Hospital Malmo, S 205 02 Malmo, Sweden
    Mol Immunol 37:445-53. 2000
    ..In conclusion, a cluster of positively charged amino acid residues at the CCP1-CCP2 interface is identified as functionally important in the regulation of the C3-convertase by C4BP...
  26. ncbi Structural requirements for the complement regulatory activities of C4BP
    A M Blom
    Lund University Wallenberg Laboratory, Department of Clinical Chemistry, University Hospital Malmo, S 205 02 Malmo, Sweden
    J Biol Chem 276:27136-44. 2001
    ..The results presented here elucidate the structural requirements of individual CCPs of C4BP, as well as their spatial arrangements within and between subunits for expression of full functional activity...
  27. doi COMP-C3b complexes in rheumatoid arthritis with severe extraarticular manifestations
    Kaisa E Happonen
    From the Department of Laboratory Medicine, Section of Medical Protein Chemistry, and the Department of Clinical Sciences, Section of Rheumatology, Lund University, Malmö the Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden the Oslo University Hospital Rikshospitalet, and University of Oslo, Oslo the Research Laboratory Nordland Hospital, and University of Tromsø, Tromsø, Norway
    J Rheumatol 40:2001-5. 2013
    ..To investigate biomarker patterns in rheumatoid arthritis (RA) with extraarticular manifestations...
  28. doi Pharmacokinetics, tolerability, and preliminary efficacy of paquinimod (ABR-215757), a new quinoline-3-carboxamide derivative: studies in lupus-prone mice and a multicenter, randomized, double-blind, placebo-controlled, repeat-dose, dose-ranging study in
    Anders A Bengtsson
    Lund University, Lund, Sweden
    Arthritis Rheum 64:1579-88. 2012
    ....
  29. ncbi Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis
    Kutty Selva Nandakumar
    Medical Inflammation Research, Lund University, Lund, Sweden
    Eur J Immunol 37:2973-82. 2007
    ..Hence, the asparagine-linked glycan on IgG plays a crucial role in the development of arthritis...
  30. doi A candidate gene approach to ANCA-associated vasculitis reveals links to the C3 and CTLA-4 genes but not to the IL1-Ra and Fcγ-RIIa genes
    Ulf Persson
    Departments of Nephrology, Lund University, Lund, Sweden
    Kidney Blood Press Res 37:641-8. 2013
    ....
  31. ncbi C4b-binding protein and factor H compensate for the loss of membrane-bound complement inhibitors to protect apoptotic cells against excessive complement attack
    Leendert A Trouw
    Department of Laboratory Medicine, University Hospital Malmo, 5 20502 Malmö, Sweden
    J Biol Chem 282:28540-8. 2007
    ..In conclusion, during late apoptosis, cells acquire fluid phase complement inhibitors that compensate for the down-regulation of m-C-Reg and protect against excessive complement activation and lysis...
  32. pmc Mutations in genes encoding complement inhibitors CD46 and CFH affect the age at nephritis onset in patients with systemic lupus erythematosus
    Andreas Jönsen
    Section of Rheumatology, Department of Clinical Sciences Lund, Lund University, Kioskgatan 3, SE 221 85 Lund, Sweden
    Arthritis Res Ther 13:R206. 2011
    ..Inherited deficiencies of several complement components strongly predispose to systemic lupus erythematosus (SLE) while deficiencies of complement inhibitors are found in kidney diseases such as atypical hemolytic uremic syndrome (aHUS)...
  33. ncbi Complement deficiencies and systemic lupus erythematosus
    Lennart Truedsson
    Section of Microbiology, Immunology and Glycobiology, Department of Laboratory Medicine, Lund University, Lund, Sweden
    Autoimmunity 40:560-6. 2007
    ..On the other hand, complement also takes part in the inflammatory reaction in the disease that gives rise to the tissue and organ damage. In this review various aspects of the relation between complement and SLE are discussed...
  34. doi Multiplexing of miniaturized planar antibody arrays for serum protein profiling--a biomarker discovery in SLE nephritis
    Linn Petersson
    Dept of Immunotechnology and CREATE Health, Medicon Village, Lund University, Medicon Village, Building no 406, SE 22381 Lund, Sweden
    Lab Chip 14:1931-42. 2014
    ....
  35. ncbi Gene-environment interactions in the aetiology of systemic lupus erythematosus
    Andreas Jönsen
    Section of Rheumatology, Department of Clinical Sciences, Lund University, Lund, Sweden
    Autoimmunity 40:613-7. 2007
    ..With increased knowledge on SLE pathogenesis, the role of environmental factors and their genetic interactions may be further elucidated...
  36. pmc Serum protein profiling of systemic lupus erythematosus and systemic sclerosis using recombinant antibody microarrays
    Anders Carlsson
    Department of Immunotechnology, BMC D13, Lund University, SE 221 84 Lund, Sweden
    Mol Cell Proteomics 10:M110.005033. 2011
    ....
  37. doi Interactions of histidine-rich glycoprotein with immunoglobulins and proteins of the complement system
    G A Manderson
    Lund University, Department of Laboratory Medicine Malmö, Division of Medical, Protein Chemistry, The Wallenberg Laboratory floor 4, University Hospital, Malmo, Sweden
    Mol Immunol 46:3388-98. 2009
    ....
  38. ncbi Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C
    U Friedrich
    Department of Clinical Chemistry, Lund University, The Wallenberg Laboratory, MAS, , Sweden
    J Biol Chem 276:24122-8. 2001
    ..In conclusion, we have characterized and spatially localized the functionally important heparin/heparan sulfate-binding site of APC...
  39. ncbi Functions of human complement inhibitor C4b-binding protein in relation to its structure
    Anna M Blom
    Lund University, University Hospital Malmo, S 205 02 Malmo, Sweden
    Arch Immunol Ther Exp (Warsz) 52:83-95. 2004
    ....
  40. doi Recombinant antibody microarray for profiling the serum proteome of SLE
    Carl A K Borrebaeck
    Department of Immunotechnology and CREATE Health, Lund University, Lund, Sweden
    Methods Mol Biol 1134:67-78. 2014
    ..The methodology provides unique opportunities for delineating serum biomarkers reflecting SLE, thus paving the way for improved diagnosis, classification, and prognosis. ..
  41. pmc Selective IgA deficiency in autoimmune diseases
    Ning Wang
    Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden
    Mol Med 17:1383-96. 2011
    ..This indicates a possible common genetic background. In this review, we present suggestive evidence for a shared genetic predisposition between these disorders...
  42. pmc Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3
    Michal Potempa
    Lund University, Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Malmo, Sweden
    PLoS Pathog 5:e1000316. 2009
    ..Taken together, the newly characterized interpain A proteinase appears to be an important virulence factor of P. intermedia...
  43. ncbi Molecular characterization of the interaction between porins of Neisseria gonorrhoeae and C4b-binding protein
    Hanna Jarva
    Department of Laboratory Medicine, Division of Medical Protein Chemistry, University of Lund, Malmo, Sweden
    J Immunol 179:540-7. 2007
    ..Only those recombinant mutant C4BPs that bound to bacteria rescued them from 100% killing by rhesus serum, thereby providing a functional correlate for the binding studies and highlighting C4BP function in gonococcal serum resistance...
  44. pmc Short leucine-rich glycoproteins of the extracellular matrix display diverse patterns of complement interaction and activation
    Andreas P Sjöberg
    Department of Laboratory Medicine, University Hospital Malmo, Lund University, Malmo, Sweden
    Mol Immunol 46:830-9. 2009
    ....
  45. ncbi The extracellular matrix and inflammation: fibromodulin activates the classical pathway of complement by directly binding C1q
    Andreas Sjöberg
    Department of Laboratory Medicine, University Hospital Malmo, BMC, Lund University, Sweden
    J Biol Chem 280:32301-8. 2005
    ..Taken together our observations indicate fibromodulin as one factor involved in the sustained inflammation of the joint...
  46. doi Complement in the immunopathogenesis of rheumatic disease
    Gunnar Sturfelt
    Section of Rheumatology, Department of Clinical Sciences, Lund University, Kioskgatan 3, SE 221 85 Lund, Sweden gunnar sturfelt med lu se
    Nat Rev Rheumatol 8:458-68. 2012
    ..Potential approaches to therapeutic intervention that focus on regulating complement activities in these disorders are also considered...
  47. ncbi Homozygosity for the IgG2 subclass allotype G2M(n) protects against severe infection in hereditary C2 deficiency
    Goran Jonsson
    Department of Infectious Diseases, University Hospital of Lund, SE 221 85 Lund, Sweden
    J Immunol 177:722-8. 2006
    ..In conclusion, the findings indicated that efficient Ab responses to polysaccharides are protective against severe infection in C2D. Implications with regard to vaccination should be considered...
  48. doi Complement inhibitor C4b-binding protein in primary Sjögren's syndrome and its association with other disease markers
    A Foltyn Zadura
    Department of Laboratory Medicine, Lund University, Lund, Sweden
    Scand J Immunol 69:374-80. 2009
    ....
  49. ncbi Bordetella pertussis binds to human C4b-binding protein (C4BP) at a site similar to that used by the natural ligand C4b
    K Berggård
    Department of Medical Microbiology, Dermatology and Infection, Lund University, Lund, Sweden
    Eur J Immunol 31:2771-80. 2001
    ..pertussis selectively bound C4BP in human plasma, indicating that binding occurs also in vivo. Together, these findings indicate that B. pertussis exploits a site in C4BP, resembling that used by the natural ligand C4b...
  50. ncbi CCP1-4 of the C4b-binding protein alpha-chain are required for factor I mediated cleavage of complement factor C3b
    Anna M Blom
    The Wallenberg Laboratory, Department of Clinical Chemistry, Lund University, University Hospital Malmo, S 205 02 Malmo, Sweden
    Mol Immunol 39:547-56. 2003
    ..However, in both cases 1,000-fold molar excess of C4BP over factor H (FH), well known inhibitor of the alternative pathway, was required to obtain the same effect...
  51. ncbi The binding of protein S and the protein S-C4BP complex to neutrophils is apoptosis dependent
    Joanna H Webb
    Division of Clinical Chemistry, Department of Laboratory Medicine, Lund University, Sweden
    Blood Coagul Fibrinolysis 14:355-9. 2003
    ..Thus, we conclude that binding of protein S and the C4BP-protein S complex to neutrophils is not cell specific, but rather apoptosis dependent...
  52. ncbi Role of CCP2 of the C4b-binding protein beta-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies
    Joanna H Webb
    Division of Clinical Chemistry, Department of Laboratory Medicine, Lund University, Sweden
    Eur J Biochem 270:93-100. 2003
    ..Taken together, the results suggest that the role of CCP2 in protein S binding is to orient and stabilize CCP1 rather than to be directly part of the binding site...
  53. doi Vitronectin binds to the head region of Moraxella catarrhalis ubiquitous surface protein A2 and confers complement-inhibitory activity
    Birendra Singh
    Medical Microbiology, Department of Laboratory Medicine, University Hospital Malmo, Lund University, Malmo, Sweden
    Mol Microbiol 75:1426-44. 2010
    ..Our study directly reveals the binding mode between the N-terminal domain of UspA2 and the C-terminal part of vitronectin and thus sheds light upon the mechanism of M. catarrhalis-dependent serum resistance...
  54. doi Nontypeable Haemophilus influenzae protein E binds vitronectin and is important for serum resistance
    Teresia Hallström
    Department of Laboratory Medicine, Lund University, University Hospital Malmo, Malmo, Sweden
    J Immunol 183:2593-601. 2009
    ..Our findings show that PE, by binding vitronectin, may play an important role in NTHi pathogenesis...
  55. doi Complement activation and plasma levels of C4b-binding protein in critical limb ischemia patients
    Myriam Martin
    University of Lund, Department of Laboratory Medicine, Malmo University Hospital, Malmo, Sweden
    J Vasc Surg 50:100-6. 2009
    ..The hypothesis of this study was that levels of complement activation and C4BP are predictive for the severity of the disease and that their measurement might be of clinical advantage...
  56. ncbi Backcross and partial advanced intercross analysis of nonobese diabetic gene-mediated effects on collagen-induced arthritis reveals an interactive effect by two major loci
    Anna Karin B Lindqvist
    Medical Inflammation Research, Lund University, Lund, Sweden
    J Immunol 177:3952-9. 2006
    ..These data show that not only arthritis but also C5-dependent complement activity is dominantly suppressed, confirming that C5 is one of the major genes explaining the Cia2 effect...
  57. ncbi Interaction with C4b-binding protein contributes to nontypeable Haemophilus influenzae serum resistance
    Teresia Hallström
    Department of Laboratory Medicine, Lund University, Malmo University Hospital, Malmo, Sweden
    J Immunol 178:6359-66. 2007
    ..influenzae retained its cofactor activity as determined by analysis of C3b and C4b degradation. Taken together, NTHi interferes with the classical complement activation pathway by binding to C4BP...
  58. ncbi Non-small cell lung cancer cells produce a functional set of complement factor I and its soluble cofactors
    Marcin Okroj
    Lund University, Department of Laboratory Medicine, Section of Clinical Chemistry, University Hospital, S 205 02 Malmo, Sweden
    Mol Immunol 45:169-79. 2008
    ....
  59. doi Native, amyloid fibrils and beta-oligomers of the C-terminal domain of human prion protein display differential activation of complement and bind C1q, factor H and C4b-binding protein directly
    Andreas P Sjöberg
    Lund University, Department of Laboratory Medicine, Division of Medical Protein Chemistry, Wallenberg Laboratory Floor 4, UMAS, 205 02 Malmo, Sweden
    Mol Immunol 45:3213-21. 2008
    ..Taken together the present study indicates that complement activation may be an important factor in human prion diseases, suggesting that complement induced activities may prove relevant therapeutic targets...
  60. ncbi A novel non-synonymous polymorphism (p.Arg240His) in C4b-binding protein is associated with atypical hemolytic uremic syndrome and leads to impaired alternative pathway cofactor activity
    Anna M Blom
    Lund University, Department of Laboratory Medicine, Malmo University Hospital, Malmo, Sweden
    J Immunol 180:6385-91. 2008
    ..Three of the patients carry also mutations in membrane cofactor protein and factor H strengthening the hypothesis that individuals may carry multiple susceptibility factors with an additive effect on the risk of developing aHUS...
  61. ncbi Haemophilus influenzae interacts with the human complement inhibitor factor H
    Teresia Hallström
    Medical Microbiology, Department of Laboratory Medicine, Lund University, Malmo University Hospital, Sweden
    J Immunol 181:537-45. 2008
    ..In contrast to incubation with active complement, H. influenzae had a reduced survival in FH-depleted human serum, thus demonstrating that FH mediates a protective role at the bacterial surface...
  62. ncbi The C4b-binding protein-protein S complex inhibits the phagocytosis of apoptotic cells
    Lena Kask
    Lund University, The Wallenberg Laboratory, Department of Clinical Chemistry, University Hospital Malmö S 205 02 Malmö, Sweden
    J Biol Chem 279:23869-73. 2004
    ..Although the presence of the C4BP-PS complex on apoptotic cells may lead to decreased phagocytosis, it may still be beneficial to the host, since it could prevent secondary necrosis because it inhibits further complement attack...
  63. pmc Binding of complement inhibitor C4b-binding protein contributes to serum resistance of Porphyromonas gingivalis
    Michal Potempa
    Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Lund University, Malmo, Sweden
    J Immunol 181:5537-44. 2008
    ....
  64. doi Characterization of the complement inhibitory function of rhesus rhadinovirus complement control protein (RCP)
    Marcin Okroj
    Department of Laboratory Medicine, Lund University, University Hospital Malmo, Malmo S 20502, Sweden
    J Biol Chem 284:505-14. 2009
    ..This makes RRV a superior model for future in vivo investigations of complement evasion, as RCP does not play a supportive role in viral attachment as KCP does...
  65. doi Hypoxia increases susceptibility of non-small cell lung cancer cells to complement attack
    Marcin Okroj
    Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital, UMAS, Lund University, Entrance 46, 205 02 Malmo, Sweden
    Cancer Immunol Immunother 58:1771-80. 2009
    ..Taken together, our results imply that under hypoxic conditions NSCLC give up some of their available defense mechanisms and become more prone to complement attack...
  66. pmc Separation of decay-accelerating and cofactor functional activities of Kaposi's sarcoma-associated herpesvirus complement control protein using monoclonal antibodies
    Linda Mark
    Medical Protein Chemistry Group, Lund University, University Hospital Malmo, Malmo, Sweden
    Immunology 123:228-38. 2008
    ..These results show that, while KCP is a multifunctional protein, these activities do not completely overlap and can be isolated through incubation with monoclonal antibodies...
  67. ncbi Complement inhibitor C4b-binding protein interacts directly with small glycoproteins of the extracellular matrix
    Kaisa E Happonen
    Department of Laboratory Medicine, Wallenberg Laboratory, University Hospital Malmo, Lund University, Malmo, Sweden
    J Immunol 182:1518-25. 2009
    ....
  68. ncbi Complement inhibitor C4b-binding protein-friend or foe in the innate immune system?
    Anna M Blom
    The Wallenberg Laboratory, Department of Clinical Chemistry, University Hospital Malmo, Lund University, S 205 02 Malmo, Sweden
    Mol Immunol 40:1333-46. 2004
    ..This contributes to the serum resistance and the pathogenicity of these bacteria. In this review, the structural requirements and functional importance of the interactions between C4BP and its various ligands are discussed...
  69. ncbi A mutation in factor I that is associated with atypical hemolytic uremic syndrome does not affect the function of factor I in complement regulation
    Sara C Nilsson
    Lund University, Department of Laboratory Medicine, University Hospital Malmo, S 205 02 Malmo, Sweden
    Mol Immunol 44:1835-44. 2007
    ..In conclusion, despite the association between the heterozygous mutation in FI and aHUS we did not observe any abnormalities in the function of FI regarding complement regulation...
  70. pmc Molecular characterization of the rhesus rhadinovirus (RRV) ORF4 gene and the RRV complement control protein it encodes
    Linda Mark
    Department of Laboratory Medicine, Lund University, University Hospital Malmo, Sweden
    J Virol 81:4166-76. 2007
    ..These data provide the foundation for studies into the biological significance of gammaherpesvirus complement regulatory proteins in a tractable, non-human primate model...
  71. ncbi The factor H variant associated with age-related macular degeneration (His-384) and the non-disease-associated form bind differentially to C-reactive protein, fibromodulin, DNA, and necrotic cells
    Andreas P Sjöberg
    Department of Laboratory Medicine, University Hospital Malmo, Lund University, S 205 02 Malmo, Sweden
    J Biol Chem 282:10894-900. 2007
    ..The variations in binding affinity of the two alleles indicate that complement activation and local inflammation in response to different targets will differ between His/His and Tyr/Tyr homozygotes...
  72. pmc Analysis of binding sites on complement factor I that are required for its activity
    Sara C Nilsson
    Department of Laboratory Medicine, Medical Protein Chemistry, Malmo University Hospital, Lund University, S 205 02 Malmo, Sweden
    J Biol Chem 285:6235-45. 2010
    ..These same mutants also showed impaired binding to C3met. In conclusion, the FIMAC domain appears to harbor the main binding sites important for the ability of FI to degrade C4b and C3b...
  73. doi Clinical isolates of Streptococcus pneumoniae bind the complement inhibitor C4b-binding protein in a PspC allele-dependent fashion
    Antoine Dieudonné-Vatran
    Department of Laboratory Medicine, Medical Microbiology, Lund University, Malmo, Sweden
    J Immunol 182:7865-77. 2009
    ..4) as a major ligand for C4BP, revealing a new function for this important pneumococcal virulence factor. Thus pneumococci exploit host C4BP for complement evasion in a PspC allele-dependent manner...
  74. ncbi Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system
    Katarzyna Popadiak
    Lund University, Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Malmo, Sweden
    J Immunol 178:7242-50. 2007
    ..At later stages of infection the concentration of proteases is high enough to destroy complement factors and thus render the bacteria resistant to the bactericidal activity of complement...
  75. ncbi Rheumatoid arthritis and the complement system
    Marcin Okroj
    Lund University, Department of Laboratory Medicine, University Hospital Malmo, Malmo, Sweden
    Ann Med 39:517-30. 2007
    ..Success of complement inhibition in the experimental models described so far encourages novel therapeutic approaches to the treatment of human RA...
  76. ncbi Viral heparin-binding complement inhibitors--a recurring theme
    Anna M Blom
    Lund University, Department of Laboratory Medicine, The Wallenberg Laboratory, 4th Floor, Malmo University Hospital, Entrance 46, S 205 02 Malmo, Sweden
    Adv Exp Med Biol 598:105-25. 2007
  77. ncbi Ionic binding of C3 to the human pathogen Moraxella catarrhalis is a unique mechanism for combating innate immunity
    Therese Nordstrom
    Medical Microbiology, Lund University, Malmo University Hospital, Malmo, Sweden
    J Immunol 175:3628-36. 2005
    ..Interestingly, among the large number of bacteria analyzed, only M. catarrhalis has this unique ability to interfere with the innate immune system of complement by binding C3...
  78. pmc Structural stability and heat-induced conformational change of two complement inhibitors: C4b-binding protein and factor H
    Lena Kask
    Lund University, The Wallenberg Laboratory, Department of Clinical Chemistry, University Hospital Malmo, S 205 02 Malmo, Sweden
    Protein Sci 13:1356-64. 2004
    ..These findings are of interest because CCP-containing complement inhibitors have been proposed as clinical agents to be used to control unwanted complement activation that contributes to many diseases...
  79. ncbi Effects of zinc on factor I cofactor activity of C4b-binding protein and factor H
    Anna M Blom
    Department of Clinical Chemistry, Lund University, University Hospital Malmo, S 205 02, Malmo, Sweden
    Arch Biochem Biophys 418:108-18. 2003
    ..Inhibition of the reaction seen at higher concentrations is due to aggregation of C4b/C3b...
  80. doi Structural basis and functional effects of the interaction between complement inhibitor C4b-binding protein and DNA
    Marcin Okroj
    Lund University, Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Malmo, Sweden
    Mol Immunol 46:62-9. 2008
    ..Taken together these results reinforce the case for the occupation of some of the seven arms of C4BP in a multivalent interaction with DNA or surface bound glycosaminoglycans while other arms engage C4b or C3b...
  81. ncbi Mutations in alpha-chain of C4BP that selectively affect its factor I cofactor function
    Anna M Blom
    Lund University Department of Clinical Chemistry, University Hospital Malmo, The Wallenberg Laboratory, S 205 02 Malmo, Sweden and INSERM U428, Universite Paris V, Paris 75006, France
    J Biol Chem 278:43437-42. 2003
    ..It appears that C4b and C3b do not undergo the same conformational changes upon binding to the C4BP mutants as during the interaction with the wild type C4BP, which then results in the observed loss of the cofactor activity...