Fernando J Pena

Summary

Affiliation: University of Extremadura
Country: Spain

Publications

  1. doi request reprint New flow cytometry approaches in equine andrology
    Fernando J Pena
    University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Caceres, Spain Electronic address
    Theriogenology 86:366-72. 2016
  2. ncbi request reprint Effect of vulvomucosal injection of D-cloprostenol at weaning and at insemination on reproductive performance of sows during the low fertility summer season under field conditions
    F J Pena
    Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Extremadura, Av de la Universidad s n, 10071, Caceres, Spain
    Anim Reprod Sci 68:77-83. 2001
  3. doi request reprint Dissecting the molecular damage to stallion spermatozoa: the way to improve current cryopreservation protocols?
    F J Pena
    Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Spain
    Theriogenology 76:1177-86. 2011
  4. doi request reprint Mitochondria in mammalian sperm physiology and pathology: a review
    F J Pena
    Laboratory of Spermatology, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Reprod Domest Anim 44:345-9. 2009
  5. ncbi request reprint Detecting subtle changes in sperm membranes in veterinary andrology
    Fernando J Pena
    Department of Herd Health and Medicine, Faculty of Veterinary Medicine, Avd de la Universidad s n, Caceres 10071, Spain
    Asian J Androl 9:731-7. 2007
  6. ncbi request reprint Semen technologies in dog breeding: an update
    F J Pena
    Reproduction and Obstetrics, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Reprod Domest Anim 41:21-9. 2006
  7. ncbi request reprint Endometrial adenocarcinoma and mucometra in a 6-year-old Alaska Malamute dog
    F J Pena
    Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Reprod Domest Anim 41:189-90. 2006
  8. ncbi request reprint Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa
    F J Pena
    Section of Animal Reproduction, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s n, 10071 Caceres, Spain
    Anim Reprod Sci 97:74-83. 2007
  9. ncbi request reprint Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to postthaw quality
    Fernando J Pena
    Section of Animal Reproduction and Obstetrics, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Caceres, Spain
    J Androl 26:716-23. 2005
  10. ncbi request reprint Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate
    F J Pena
    Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences SLU, Box 7039, SE 750 07 Uppsala, Sweden
    Anim Reprod Sci 78:85-98. 2003

Collaborators

  • Heriberto Rodriguez-Martinez
  • F Saravia
  • Isaac Jardin
  • Gines M Salido
  • J C Samper
  • M C Gil
  • Anders Johannisson
  • Patricia Martín Muñoz
  • Cristina Ortega Ferrusola
  • Jose A Tapia
  • María Plaza Dávila
  • Juan M Gallardo Bolaños
  • Carolina M Balao da Silva
  • Ines M Aparicio
  • Alvaro Miro-Moran
  • Antolín Morillo Rodríguez
  • Beatriz Macías García
  • Cristina Ortega-Ferrusola
  • Luis Anel
  • Luis Anel Lopez
  • Mercedes Alvarez Garcia
  • Chiara Del Petre
  • Paulino de Paz Cabello
  • Carolina Balao da Silva C
  • Heriberto Rodriguez Martinez
  • Guillermo Vizuete
  • Antolin Morillo Rodriguez
  • Carolina Maria Balao da Silva
  • Juan Maria Gallardo Bolaños
  • Heriberto Rodríguez Martínez
  • Alvaro Miró Moran
  • Alvaro Miro Moran
  • Lauro González Fernández
  • Beatriz Macías-García
  • Antolín Morillo-Rodriguez
  • Lauro Gonzalez-Fernandez
  • George Stilwell
  • Juan M Gallardo-Bolaños

Detail Information

Publications23

  1. doi request reprint New flow cytometry approaches in equine andrology
    Fernando J Pena
    University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Caceres, Spain Electronic address
    Theriogenology 86:366-72. 2016
    ..In this review, recent advances in the evaluation of stallion spermatozoa will be discussed. These new techniques in flow cytometry are able to simultaneously measure damage to different sperm regions and/or changes in functionality. ..
  2. ncbi request reprint Effect of vulvomucosal injection of D-cloprostenol at weaning and at insemination on reproductive performance of sows during the low fertility summer season under field conditions
    F J Pena
    Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Extremadura, Av de la Universidad s n, 10071, Caceres, Spain
    Anim Reprod Sci 68:77-83. 2001
    ..001). Fertility for treated sows was also significantly increased by the treatment (P<0.01). However, neither the percentage of inseminated sows that farrowed nor litter size was affected by treatment...
  3. doi request reprint Dissecting the molecular damage to stallion spermatozoa: the way to improve current cryopreservation protocols?
    F J Pena
    Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Spain
    Theriogenology 76:1177-86. 2011
    ..Increased knowledge about the molecular injuries that occur during cryopreservation may lead to improved protective techniques and thus to further improvements in fertility in the current decade...
  4. doi request reprint Mitochondria in mammalian sperm physiology and pathology: a review
    F J Pena
    Laboratory of Spermatology, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Reprod Domest Anim 44:345-9. 2009
    ....
  5. ncbi request reprint Detecting subtle changes in sperm membranes in veterinary andrology
    Fernando J Pena
    Department of Herd Health and Medicine, Faculty of Veterinary Medicine, Avd de la Universidad s n, Caceres 10071, Spain
    Asian J Androl 9:731-7. 2007
    ..Using phospholipid transposition tests, early changes in membrane permeability and fluidity can be assessed in a large number of spermatozoa using fluorescent probes in combination with flow cytometry...
  6. ncbi request reprint Semen technologies in dog breeding: an update
    F J Pena
    Reproduction and Obstetrics, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Reprod Domest Anim 41:21-9. 2006
    ..New techniques to evaluate early membrane changes have also been recently developed in our laboratory...
  7. ncbi request reprint Endometrial adenocarcinoma and mucometra in a 6-year-old Alaska Malamute dog
    F J Pena
    Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Reprod Domest Anim 41:189-90. 2006
    ..The mass was encapsulated by the omentum, but areas of necrosis and calcification were identified. Histopathological diagnosis was endometrial adenocarcinoma...
  8. ncbi request reprint Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa
    F J Pena
    Section of Animal Reproduction, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s n, 10071 Caceres, Spain
    Anim Reprod Sci 97:74-83. 2007
    ..440, p<0.01), indicating this new triple staining can be used to safely disclose among ejaculates prior to freezing...
  9. ncbi request reprint Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to postthaw quality
    Fernando J Pena
    Section of Animal Reproduction and Obstetrics, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Caceres, Spain
    J Androl 26:716-23. 2005
    ....
  10. ncbi request reprint Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate
    F J Pena
    Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences SLU, Box 7039, SE 750 07 Uppsala, Sweden
    Anim Reprod Sci 78:85-98. 2003
    ..The magnitude of the effect appeared, however, to be dependent of the fraction of the ejaculate considered...
  11. ncbi request reprint Assessment of fresh and frozen-thawed boar semen using an Annexin-V assay: a new method of evaluating sperm membrane integrity
    Fernando J Pena
    Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences SLU, Box 7039, SE 750 07 Uppsala, Sweden
    Theriogenology 60:677-89. 2003
    ..The finding that fewer spermatozoa in Portion I of the ejaculate showed early apoptosis post-freezing, suggests boar spermatozoa in this portion of the seminal plasma are less sensitive to the stress induced by cryopreservation...
  12. ncbi request reprint Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation
    F J Pena
    Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences SLU, SE 750 07 Uppsala, Sweden
    Theriogenology 61:63-70. 2004
    ..In conclusion, HA appeared to preserve post-thaw spermatozoa viability in vitro and maintained membrane stability after cryopreservation...
  13. ncbi request reprint Antioxidant supplementation of boar spermatozoa from different fractions of the ejaculate improves cryopreservation: changes in sperm membrane lipid architecture
    F J Pena
    Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Zygote 12:117-24. 2004
    ....
  14. ncbi request reprint Do different portions of the boar ejaculate vary in their ability to sustain cryopreservation?
    F J Pena
    Animal Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Extremadura, Avda de la Universidad s n, 10071 Caceres, Spain
    Anim Reprod Sci 93:101-13. 2006
    ..It is concluded that the identification of the ejaculate portion more suitable to sustain cryopreservation in each individual boar may be a readily applicable and easy technique to diminish variation in sperm freezability among boars...
  15. ncbi request reprint A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa
    F J Pena
    Section of Animal Reproduction, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s n, 10071 Caceres, Spain
    Int J Androl 28:107-14. 2005
    ..The new assay stained all sperm sub-populations present in the sample, making it especially suitable for both fluorescence microscopy and flow cytometry, facilitating the exclusion of debris and egg-yolk particles when using FC...
  16. doi request reprint Phosphorylated AKT preserves stallion sperm viability and motility by inhibiting caspases 3 and 7
    Juan M Gallardo Bolaños
    Laboratory of Equine Reproduction and Equine SpermatologyFaculty of Veterinary Medicine, Veterinary Teaching HospitalDepartment of PhysiologyFaculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s n, 10003 Caceres, SpainDepartment of Clinical and Experimental MedicineFaculty of Health Sciences, Linkoping University, Linkoping, Sweden
    Reproduction 148:221-35. 2014
    ..It is concluded that AKT maintains the membrane integrity of ejaculated stallion spermatozoa, presumably by inhibiting caspases 3 and 7, which prevents the progression of spermatozoa to an incomplete form of apoptosis...
  17. pmc Identification and function of exchange proteins activated directly by cyclic AMP (Epac) in mammalian spermatozoa
    Alvaro Miro-Moran
    Cell Physiology Research Group, University of Extremadura, Caceres, Spain
    PLoS ONE 7:e37713. 2012
    ....
  18. pmc Autophagy and apoptosis have a role in the survival or death of stallion spermatozoa during conservation in refrigeration
    Juan M Gallardo Bolaños
    Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Caceres, Spain
    PLoS ONE 7:e30688. 2012
    ....
  19. doi request reprint Melatonin reduces lipid peroxidation and apoptotic-like changes in stallion spermatozoa
    Carolina M Balao da Silva
    Laboratory of Equine Reproduction, Faculty of Veterinary Medicine, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    J Pineal Res 51:172-9. 2011
    ..It is concluded that melatonin is a useful tool to improve the quality of stored stallion sperm, increasing their life span and reducing premature aging, this likely relates to melatonin's antioxidant properties...
  20. pmc Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility
    María Plaza Dávila
    Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    PLoS ONE 10:e0138777. 2015
    ..These data also indicate that increased hydrogen peroxide originating in the mitochondria is a mechanism involved in stallion sperm senescence...
  21. doi request reprint The mitochondria of stallion spermatozoa are more sensitive than the plasmalemma to osmotic-induced stress: role of c-Jun N-terminal kinase (JNK) pathway
    Beatriz Macías García
    Veterinary Teaching Hospital, Laboratory of Equine Reproduction, Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s n, Caceres, Spain
    J Androl 33:105-13. 2012
    ....
  22. doi request reprint Depletion of Intracellular Thiols and Increased Production of 4-Hydroxynonenal that Occur During Cryopreservation of Stallion Spermatozoa Lead to Caspase Activation, Loss of Motility, and Cell Death
    Patricia Martín Muñoz
    Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Biol Reprod 93:143. 2015
    ..The accelerated senescence of thawed spermatozoa is related to oxidative and electrophilic stress induced by increased production of 4-hydroxynoneal in thawed samples once intracellular thiols are depleted. ..
  23. doi request reprint Caspase 3 Activity and Lipoperoxidative Status in Raw Semen Predict the Outcome of Cryopreservation of Stallion Spermatozoa
    Patricia Martín Muñoz
    Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Caceres, Spain
    Biol Reprod 95:53. 2016
    ..900, P < 0.01]. Moreover, we described for the first time the presence of 8-ISO-PGF2alpha in stallion spermatozoa and revealed the importance of considering different markers of oxidative stress. ..