Jose V Castell
Affiliation: University of Valencia
- Allergic hepatitis induced by drugsJose V Castell
Unit for Experimental Hepatology, Research Centre, University Hospital La Fe, Spain
Curr Opin Allergy Clin Immunol 6:258-65. 2006....
- Metabolism and bioactivation of toxicants in the lung. The in vitro cellular approachJose V Castell
Research Center, University Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
Exp Toxicol Pathol 57:189-204. 2005..Genetic manipulation of lung-derived cells allowing them to re-express key biotransformation enzymes appear to be a promising strategy to improve their functionality and metabolic performance...
- Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytesGabriela Perez
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
Toxicol In Vitro 17:643-9. 2003..This technique can be of value, to considerably simplify the identification of drug candidates with potential CYP inducing ability in man...
- Hepatocyte cell lines: their use, scope and limitations in drug metabolism studiesJose V Castell
University Hospital La Fe, Research Centre, Avda, Campanar 21, E 46009 Valencia, Spain
Expert Opin Drug Metab Toxicol 2:183-212. 2006..This paper reviews the features of liver-derived cell lines, their suitability for drug metabolism studies as well as the state-of-the-art of the strategies pursued in order to generate metabolically competent hepatic cell lines...
- Down-regulation of human CYP3A4 by the inflammatory signal interleukin-6: molecular mechanism and transcription factors involvedRamiro Jover
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, E 46009 Valencia, Spain
FASEB J 16:1799-801. 2002..From a clinical point of view, these findings could be relevant in the development of therapeutic cytokines with a less repressive effect on hepatic drug-metabolizing enzymes...
- Drug metabolism by cultured human hepatocytes: how far are we from the in vivo reality?Xavier Ponsoda
Unit of Experimental Hepatology, Research Centre, University Hospital La Fe, Avda Campanar 21, 46009 Valencia, Spain
Altern Lab Anim 32:101-10. 2004..95). Overall, although differing among assays, the in vitro/in vivo metabolism data for each patient were surprisingly similar. Thus, the variability observed in vitro appears to reflect genuine phenotypic variability among the donors...
- HepG2 cells simultaneously expressing five P450 enzymes for the screening of hepatotoxicity: identification of bioactivable drugs and the potential mechanism of toxicity involvedLaia Tolosa
Unidad de Hepatologia Experimental, Centro de Investigacion, Instituto de Investigación Sanitaria Hospital La Fe, Valencia, Avda de Campanar 21, 46009 Valencia, Spain
Arch Toxicol 87:1115-27. 2013..Overall, our results indicate that this assay may be a suitable new in vitro approach for early screening of compounds to identify bioactivable hepatotoxins and the mechanism(s) involved in their toxicity...
- Strategies and molecular probes to investigate the role of cytochrome P450 in drug metabolism: focus on in vitro studiesM Teresa Donato
Unit of Experimental Hepatology, Research Centre, University Hospital La Fe and Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain
Clin Pharmacokinet 42:153-78. 2003..Primary cultured hepatocytes are still the unique in vitro model that allows global examination of the inductive potential of a drug...
- An update on metabolism studies using human hepatocytes in primary cultureMaría José Gómez-Lechón
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, 46009 Valencia, Spain
Expert Opin Drug Metab Toxicol 4:837-54. 2008..The variability observed in human hepatocytes reflects the existing phenotypic heterogeneity of cytochrome P450 expression in human liver...
- Presence of fibronectin peptides in saliva of patients with Sjögren's syndrome: a potential indicator of salivary gland destructionFrancisco J Silvestre
Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, Valencia, Spain
Med Oral Patol Oral Cir Bucal 14:e365-70. 2009..The purpose of this preliminary study was to monitor the degree of destruction of salivary glands in Sjögren disease by the detection of fibronectin peptides in patients' saliva...
- Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profileMarta Moya
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Valencia, Spain
Chem Biol Interact 184:376-87. 2010..Our results also suggest that human hepatocytes cultured with free fatty acids offer a highly valuable in vitro system to investigate the pathogenesis and therapeutics of the human fatty liver...
- Liver cell culture techniquesJose V Castell
Unit of Experimental Hepatology, University Hospital La Fe, Valencia, Spain
Methods Mol Biol 481:35-46. 2009..Practical details on how to carry out these cultures and to assess their functionality (CYP activity and ureogenesis) are discussed in this chapter...
- Transcriptional regulation and expression of CYP3A4 in hepatocytesCelia P Martinez-Jimenez
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, 46009 Valencia, Spain
Curr Drug Metab 8:185-94. 2007..However, other hepatic models should be considered in drug development for screening purposes owing to the limited availability of human hepatocytes...
- Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROSM Jose Gomez-Lechon
Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
Biochem Pharmacol 66:2155-67. 2003....
- Diclofenac induces apoptosis in hepatocytesMaría José Gómez-Lechón
Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
Toxicol In Vitro 17:675-80. 2003..Finally, the differential cytotoxic and apoptotic effect produced in hepatocytes and non-metabolizing hepatoma cells suggest that CYP-mediated metabolism of diclofenac apoptosis may be related to the apoptotic effect of the drug...