Affiliation: University of Navarra
- Polyphenols and DNA Damage: A Mixed BlessingAmaya Azqueta
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Navarra, C Irunlarrea 1, 31009 Pamplona, Spain
Nutrients 8:. 2016..High concentrations can themselves induce DNA damage, as demonstrated in numerous cell culture experiments; low concentrations, on the other hand, tend to decrease DNA damage...
- Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay--a methodological overviewAmaya Azqueta
Department of Pharmacology and Toxicology, University of Navarra, C Irunlarrea 1, 31009 Pamplona, Spain Department of Nutrition, University of Oslo, PB 1046 Blindern, Oslo, Norway Electronic address
DNA Repair (Amst) 12:1007-10. 2013..By proposing standard protocols, we hope to facilitate comparison of results obtained in different laboratories...
- Enhancing the sensitivity of the comet assay as a genotoxicity test, by combining it with bacterial repair enzyme FPGAmaya Azqueta
Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
Mutagenesis 28:271-7. 2013..Triton X-100-induced breaks were secondary to cytotoxicity. The remaining compounds induced no damage. Thus, FPG enhances sensitivity of the comet assay without compromising selectivity...
- The essential comet assay: a comprehensive guide to measuring DNA damage and repairAmaya Azqueta
Department of Pharmacology and Toxicology, University of Navarra, C Irunlarrea 1, 31009 Pamplona, Spain
Arch Toxicol 87:949-68. 2013..We briefly describe the applications of this method in genotoxicity testing, human biomonitoring, and ecogenotoxicology...
- Vitamin C in cultured human (HeLa) cells: lack of effect on DNA protection and repairAmaya Azqueta
Department of Pharmacology and Toxicology, University of Navarra, C Irunlarrea 1, 31008 Pamplona, Spain
Nutrients 5:1200-17. 2013..Our aim was to investigate antioxidant/ pro-oxidant effects of vitamin C at the cellular level...
- A comparative performance test of standard, medium- and high-throughput comet assaysAmaya Azqueta
Department of Nutrition, Faculty of Medicine, University of Oslo, P O Box 1046, Blindern, 0316 Oslo, Norway
Toxicol In Vitro 27:768-73. 2013....
- The comet assay, DNA damage, DNA repair and cytotoxicity: hedgehogs are not always deadYolanda Lorenzo
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Mutagenesis 28:427-32. 2013..It is clear that 'hedgehogs' can correspond to one level on a continuum of genotoxic damage, are not diagnostic of apoptosis and should not be regarded as an indicator of cytotoxicity...
- Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisationSergey Shaposhnikov
Department of Nutrition, University of Oslo, Blindern, Oslo, Norway
Toxicol Lett 195:31-4. 2010....
- The influence of scoring method on variability in results obtained with the comet assayAmaya Azqueta
Department of Nutrition, Faculty of Medicine, University of Oslo, PO Box 1046 Blindern, 0316 Oslo, Norway
Mutagenesis 26:393-9. 2011..Overall, the degree of agreement between the scoring methods is within acceptable limits according to a Bland-Altman analysis...
- In vitro comet assay for DNA repair: a warning concerning application to cultured cellsAmaya Azqueta
Department of Nutrition, University of Oslo, Norway
Mutagenesis 24:379-81. 2009..Here, we describe a rigorous approach to testing for this possibility, which we recommend to anyone carrying out similar experiments...
- Towards a more reliable comet assay: optimising agarose concentration, unwinding time and electrophoresis conditionsAmaya Azqueta
Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Mutat Res 724:41-5. 2011..human peripheral blood lymphocytes and the lymphoblastoid cell line TK-6. All these variables have marked effects on assay performance and, therefore, on the determination of DNA damage. Here we identify factors of particular importance...
- Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespanKristiane Haug
Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, Ullevål and University of Oslo, Oslo, Norway
Acta Ophthalmol 91:219-25. 2013..Storage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC...
- Effects of micronutrients on DNA repairAndrew R Collins
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Blindern, PB 1046, 0316, Oslo, Norway
Eur J Nutr 51:261-79. 2012..In addition, there are numerous investigations at the level of expression of DNA repair-related genes...
- Both base excision repair and nucleotide excision repair in humans are influenced by nutritional factorsAsgeir Brevik
Division of Environmental Medicine, Department of Chemical Toxicology, Norwegian Institute of Public Health, Oslo, Norway
Cell Biochem Funct 29:36-42. 2011..Here we have demonstrated that DNA repair is affected by diet and that modified versions of the comet assay can be used to assess activity of different DNA repair pathways in human biomonitoring studies...
- Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cellsAlice A Ramos
Department of Nutrition, University of Oslo, Oslo, Norway
J Agric Food Chem 58:7465-71. 2010..In conclusion, SO, SF, and the isolated compounds (RA and L-7-G) demonstrated chemopreventive activity by protecting cells against oxidative DNA damage and stimulating DNA repair (SO, SF, and L-7-G)...
- DNA oxidation: investigating its key role in environmental mutagenesis with the comet assayAmaya Azqueta
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Mutat Res 674:101-8. 2009..Finally, the potential use of this technique to monitor effects of contaminants in the natural environment has yet to be fully exploited...
- Comet assay to measure DNA repair: approach and applicationsAmaya Azqueta
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Navarra Pamplona, Spain
Front Genet 5:288. 2014..Studies to date have examined effects of environment, nutrition, lifestyle, and occupation, in addition to clinical investigations. ..
- Carotenoids and DNA damageAmaya Azqueta
Department of Nutrition, Food Science and Toxicology, Schools of Pharmacy and Sciences, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
Mutat Res 733:4-13. 2012..The tendency to exacerbate damage is seen mainly at high concentrations, and might be accounted for by pro-oxidant actions of these carotenoids...
- DNA repair as a biomarker in human biomonitoring studies; further applications of the comet assayAndrew R Collins
Department of Nutrition, University of Oslo, Oslo, Norway
Mutat Res 736:122-9. 2012..Exposure to genotoxic agents has in general not been found to stimulate repair. Crucial questions concerning the factors regulating repair and the causes of individual variation are as yet unanswered...
- Synthesis and biological evaluation of quinoxaline di-N-oxide derivatives with in vitro trypanocidal activitySilvia Pérez-Silanes
Department of Organic and Pharmaceutical Chemistry, University of Navarra, Irunlarrea s n, 31008 Pamplona, Spain Institute of Tropical Health, University of Navarra, Irunlarrea s n, 31008 Pamplona, Spain
Bioorg Med Chem Lett 26:903-6. 2016..5 μM. Moreover, unspecific cytotoxicity and genotoxicity studies are also reported. Compound 14 showed a SI higher than 24, whereas compound 10 was the only one that was negative in the genotoxicity screening...
- Causes of genome instability: the effect of low dose chemical exposures in modern societySabine A S Langie
Environmental Risk and Health Unit, Flemish Institute for Technological Research VITO, Boeretang 200, 2400 Mol, Belgium, Health Canada, Environmental Health Sciences and Research Bureau, Environmental Health Centre, Ottawa, Ontario K1A0K9, Canada, Department of Pathology, Kuwait University, Safat 13110, Kuwait, Department of Experimental and Clinical Medicine, University of Firenze, Florence 50134, Italy, Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Navarra, Pamplona 31009, Spain, Environmental and Molecular Toxicology, Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA, Department of Environmental and Radiological Health Sciences Food Science and Human Nutrition, College of Veterinary Medicine and Biomedical Sciences, Colorado State University Colorado School of Public Health, Fort Collins, CO 80523 1680, USA, Department of Chemicals and Radiation, Division of Environmental Medicine, Norwegian Institute of Public Health, PO Box 4404, N 0403 Oslo, Norway, Hopkins Building, School of Biological Sciences, University of Reading, Reading, United Kingdom
Carcinogenesis 36:S61-88. 2015..The overall aim is to make scientists aware of the increasing need to unravel the underlying mechanisms via which chemicals at low doses can induce genome instability and thus promote carcinogenesis. ..
- Genotoxicity of Aflatoxin B1 and Ochratoxin A after simultaneous application of the in vivo micronucleus and comet assayLaura Ana Corcuera
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
Food Chem Toxicol 76:116-24. 2015..The combined treatment reduced DNA damage in the liver and had no influence in the kidney. Altogether, these results may be indicative of an antagonistic relationship regarding the genotoxicity of both mycotoxins. ..
- Does the duration of lysis affect the sensitivity of the in vitro alkaline comet assay?José Manuel Enciso
Department of Pharmacology and Toxicology, University of Navarra, Irunlarrea 1, 31009 Pamplona, Spain
Mutagenesis 30:21-8. 2015..In conclusion, the time of lysis can be varied depending on the sensitivity needed in both versions of the assay, and a constant time of lysis should be used if results from different experiments or laboratories are to be compared...
- Assessment of DNA damage using comet assay in middle-aged overweight/obese subjects after following a hypocaloric diet supplemented with cocoa extractIdoia Ibero-Baraibar
Department of Nutrition, Food Science and Physiology, Nutrition Research Centre, University of Navarra, C Irunlarrea 1, 31008 Pamplona, Navarra, Spain
Mutagenesis 30:139-46. 2015..On the other hand, the inverse associations found between oxidised bases and some cocoa-derived metabolites suggest that a protective effect might be seen in a longer period of time or in subjects with higher baseline DNA damage...