Maria J Bonete

Summary

Affiliation: Universidad de Alicante
Country: Spain

Publications

  1. pmc Nitrogen metabolism in haloarchaea
    Maria Jose Bonete
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain
    Saline Systems 4:9. 2008
  2. ncbi request reprint Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: enzyme purification, characterisation and N-terminal sequence
    M J Bonete
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Spain
    FEBS Lett 383:227-9. 1996
  3. pmc Biodiversity of Archaea and floral of two inland saltern ecosystems in the Alto Vinalopó Valley, Spain
    Basilio Zafrilla
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, AP, 99, E 03080 Alicante, Spain
    Saline Systems 6:10. 2010
  4. ncbi request reprint Occurrence of two different glutamate dehydrogenase activities in the halophilic bacterium Salinibacter ruber
    Maria Jose Bonete
    Division of Biochemistry and Molecular Biology, University of Alicante, 03080, Alicante, Spain
    FEMS Microbiol Lett 226:181-6. 2003
  5. ncbi request reprint 2-Hydroxyacid dehydrogenase from Haloferax mediterranei, a D-isomer-specific member of the 2-hydroxyacid dehydrogenase family
    M J Bonete
    Departamento de Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, Alicante, Spain
    Biochimie 82:1143-50. 2000
  6. ncbi request reprint Alpha-amylase activity from the halophilic archaeon Haloferax mediterranei
    F Perez-Pomares
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain
    Extremophiles 7:299-306. 2003
  7. ncbi request reprint Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis
    J Esclapez
    Departamento de Agroquimica y Bioquimica, División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEBS Lett 581:837-42. 2007
  8. ncbi request reprint Heterologous overexpression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase/reductase family
    C Pire
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 200:221-7. 2001
  9. ncbi request reprint Assimilatory nitrate reductase from the haloarchaeon Haloferax mediterranei: purification and characterisation
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 204:381-5. 2001
  10. ncbi request reprint Look on the positive side! The orientation, identification and bioenergetics of 'Archaeal' membrane-bound nitrate reductases
    Rosa Maria Martinez-Espinosa
    Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain
    FEMS Microbiol Lett 276:129-39. 2007

Collaborators

Detail Information

Publications16

  1. pmc Nitrogen metabolism in haloarchaea
    Maria Jose Bonete
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain
    Saline Systems 4:9. 2008
    ..The results obtained show that this microorganism could be very attractive for bioremediation applications in those areas where high salt, nitrate and nitrite concentrations are found in ground waters and soils...
  2. ncbi request reprint Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: enzyme purification, characterisation and N-terminal sequence
    M J Bonete
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Spain
    FEBS Lett 383:227-9. 1996
    ..However, the halophilic glucose dehydrogenase appears to be a dimeric protein, in contrast to the tetrameric enzyme from the thermophile...
  3. pmc Biodiversity of Archaea and floral of two inland saltern ecosystems in the Alto Vinalopó Valley, Spain
    Basilio Zafrilla
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, AP, 99, E 03080 Alicante, Spain
    Saline Systems 6:10. 2010
    ..However, studies focused on the characterization of inland solar salterns are scarce and both the archaeal diversity and the plant communities inhabiting these environments remain poorly described...
  4. ncbi request reprint Occurrence of two different glutamate dehydrogenase activities in the halophilic bacterium Salinibacter ruber
    Maria Jose Bonete
    Division of Biochemistry and Molecular Biology, University of Alicante, 03080, Alicante, Spain
    FEMS Microbiol Lett 226:181-6. 2003
    ..A low-molecular-mass cytoplasmic fraction was found to be a highly effective activator of GDHII in the presence of high NaCl concentrations...
  5. ncbi request reprint 2-Hydroxyacid dehydrogenase from Haloferax mediterranei, a D-isomer-specific member of the 2-hydroxyacid dehydrogenase family
    M J Bonete
    Departamento de Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, Alicante, Spain
    Biochimie 82:1143-50. 2000
    ..5 and 8.5. The optimum temperature was 52 degrees C and at 65 degrees C there was a pronounced activity decrease. This new enzyme can be used for the production of D-2-hydroxycarboxylic acid...
  6. ncbi request reprint Alpha-amylase activity from the halophilic archaeon Haloferax mediterranei
    F Perez-Pomares
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain
    Extremophiles 7:299-306. 2003
    ..The enzyme displayed maximal activity at pHs from 7 to 8, and its optimal temperature was in a range from 50 degrees C to 60 degrees C. The results also implicated several prototropic groups in the catalytic reaction...
  7. ncbi request reprint Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis
    J Esclapez
    Departamento de Agroquimica y Bioquimica, División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEBS Lett 581:837-42. 2007
    ....
  8. ncbi request reprint Heterologous overexpression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase/reductase family
    C Pire
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 200:221-7. 2001
    ..This method may be useful as a general procedure for purifying other halophilic proteins from mesophilic hosts...
  9. ncbi request reprint Assimilatory nitrate reductase from the haloarchaeon Haloferax mediterranei: purification and characterisation
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 204:381-5. 2001
    ..In contrast, NADPH and NADH, which are electron donors in nitrate reductases from different non-photosynthetic bacteria, were ineffective...
  10. ncbi request reprint Look on the positive side! The orientation, identification and bioenergetics of 'Archaeal' membrane-bound nitrate reductases
    Rosa Maria Martinez-Espinosa
    Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain
    FEMS Microbiol Lett 276:129-39. 2007
    ..Finally, the bioenergetic consequences of nitrate reduction on the outside of the cytoplasmic membrane have been explored...
  11. ncbi request reprint Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Haloferax mediterranei
    J Ferrer
    Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    Acta Crystallogr D Biol Crystallogr 57:1887-9. 2001
    ..8, b = 110.9, c = 151.7 A, alpha = beta = gamma = 90 degrees. Calculated values for V(M) and consideration of the packing for both crystal forms suggests that the asymmetric units in both crystal forms contain a monomer...
  12. doi request reprint Effects of nitrogen sources on the nitrate assimilation in Haloferax mediterranei: growth kinetics and transcriptomic analysis
    Julia Esclapez
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Alicante, Spain
    FEMS Microbiol Lett 350:168-74. 2014
    ..The results are the first ever produced in relation to amino acids as nitrogen sources within the Halobacteriaceae family...
  13. ncbi request reprint Purification and characterisation of a possible assimilatory nitrite reductase from the halophile archaeon Haloferax mediterranei
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEMS Microbiol Lett 196:113-8. 2001
    ..NADPH and NADH, which are electron donors in nitrite reductases from different non-photosynthetic bacteria, were not effective with nitrite reductase from H. mediterranei...
  14. ncbi request reprint Respiratory nitrate reductase from haloarchaeon Haloferax mediterranei: biochemical and genetic analysis
    B Lledó
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    Biochim Biophys Acta 1674:50-9. 2004
    ..The absorption spectrum indicated that Nar contains Fe-S clusters. Reverse transcriptase (RT-PCR) shows that regulation of nar genes occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate...
  15. ncbi request reprint Fluorescence and quenching comparative studies of halophilic and bovine glutamate dehydrogenase
    J Ferrer
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Spain
    J Photochem Photobiol B 47:148-54. 1998
    ..The tryptophan residues in the protein are more exposed to the solvent in h-GDH than in b-GDH. The total amount of tryptophan residues is nearly the same for both enzymes...
  16. ncbi request reprint Respiratory nitrate and nitrite pathway in the denitrifier haloarchaeon Haloferax mediterranei
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Campus de San Vicente del Raspeig, 03080 Alicante, Spain
    Biochem Soc Trans 34:115-7. 2006
    ..Nar has been purified and characterized in vitro. This characterization has included protein-film voltammetry and preliminary EPR studies...