Oussama Ahrazem

Summary

Affiliation: Universidad de Castilla-La Mancha
Country: Spain

Publications

  1. pmc Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Int J Mol Sci 17:. 2016
  2. ncbi request reprint Assessing allergen levels in peach and nectarine cultivars
    Oussama Ahrazem
    Departamento de Biotecnologia, E T S Ingenieros Agronomos, Universidad Politecnica, Madrid, Spain
    Ann Allergy Asthma Immunol 99:42-7. 2007
  3. pmc Characterization of a glucosyltransferase enzyme involved in the formation of kaempferol and quercetin sophorosides in Crocus sativus
    Almudena Trapero
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Plant Physiol 159:1335-54. 2012
  4. ncbi request reprint Orange germin-like glycoprotein Cit s 1: an equivocal allergen
    Oussama Ahrazem
    Unidad de Bioquimica, Departamento de Biotecnologia, E T S Ingenieros Agronomos, UPM, Madrid, Espana
    Int Arch Allergy Immunol 139:96-103. 2006
  5. doi request reprint Developmental and stress regulation of gene expression for a 9-cis-epoxycarotenoid dioxygenase, CstNCED, isolated from Crocus sativus stigmas
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    J Exp Bot 63:681-94. 2012
  6. doi request reprint The carotenoid cleavage dioxygenase CCD2 catalysing the synthesis of crocetin in spring crocuses and saffron is a plastidial enzyme
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071, Albacete, Spain
    New Phytol 209:650-63. 2016
  7. pmc Crocins with high levels of sugar conjugation contribute to the yellow colours of early-spring flowering crocus tepals
    Angela Rubio Moraga
    Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, Spain Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, Spain
    PLoS ONE 8:e71946. 2013
  8. pmc Intron retention and rhythmic diel pattern regulation of carotenoid cleavage dioxygenase 2 during crocetin biosynthesis in saffron
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071, Albacete, Spain
    Plant Mol Biol 91:355-74. 2016
  9. doi request reprint The expression of a chromoplast-specific lycopene beta cyclase gene is involved in the high production of saffron's apocarotenoid precursors
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, E 02071 Albacete, Spain
    J Exp Bot 61:105-19. 2010
  10. ncbi request reprint Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens
    Aránzazu Palacín
    Departamento de Biotecnologia, Escuela Técnica Superior Ingenieros Agrónomos, Universidad Politecnica de Madrid, Madrid, Spain
    J Allergy Clin Immunol 117:1423-9. 2006

Collaborators

Detail Information

Publications27

  1. pmc Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Int J Mol Sci 17:. 2016
    ..This review focuses on CCDs from plants, algae, fungi, and bacteria, describing recent progress in their functions and regulatory mechanisms in relation to the different roles played by the apocarotenoids in these organisms...
  2. ncbi request reprint Assessing allergen levels in peach and nectarine cultivars
    Oussama Ahrazem
    Departamento de Biotecnologia, E T S Ingenieros Agronomos, Universidad Politecnica, Madrid, Spain
    Ann Allergy Asthma Immunol 99:42-7. 2007
    ..The lipid transfer protein Pru p 3 has been identified as a major peach fruit allergen. However, the putative peach member of the Bet v 1 family, Pru p 1, has been neither identified nor characterized...
  3. pmc Characterization of a glucosyltransferase enzyme involved in the formation of kaempferol and quercetin sophorosides in Crocus sativus
    Almudena Trapero
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Plant Physiol 159:1335-54. 2012
    ..These data reveal the involvement of UGT707B1 in the biosynthesis of flavonol-3-O-sophorosides and how significant changes in flavonoid homeostasis can be caused by the overproduction of a flavonoid-conjugating enzyme...
  4. ncbi request reprint Orange germin-like glycoprotein Cit s 1: an equivocal allergen
    Oussama Ahrazem
    Unidad de Bioquimica, Departamento de Biotecnologia, E T S Ingenieros Agronomos, UPM, Madrid, Espana
    Int Arch Allergy Immunol 139:96-103. 2006
    ..Orange allergens are virtually unknown, in spite of the large consumption of this fruit. Germin-like proteins, together with vicilins and legumins, form the cupin superfamily of plant proteins, which includes many seed allergens...
  5. doi request reprint Developmental and stress regulation of gene expression for a 9-cis-epoxycarotenoid dioxygenase, CstNCED, isolated from Crocus sativus stigmas
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    J Exp Bot 63:681-94. 2012
    ..The results provided evidence of the involvement of CstNCED in the regulation of ABA-associated processes such as flower senescence and corm dormancy in monocotyledonous saffron...
  6. doi request reprint The carotenoid cleavage dioxygenase CCD2 catalysing the synthesis of crocetin in spring crocuses and saffron is a plastidial enzyme
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071, Albacete, Spain
    New Phytol 209:650-63. 2016
    ..The specific CCD2 localization and its membrane interaction will contribute to the development of a better understanding of the mechanism of crocetin biosynthesis and regulation in the chromoplast. ..
  7. pmc Crocins with high levels of sugar conjugation contribute to the yellow colours of early-spring flowering crocus tepals
    Angela Rubio Moraga
    Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, Spain Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, Spain
    PLoS ONE 8:e71946. 2013
    ..The identification of tepals as new sources of crocins is of special interest given their wide range of applications in medicine, cosmetics and colouring industries. ..
  8. pmc Intron retention and rhythmic diel pattern regulation of carotenoid cleavage dioxygenase 2 during crocetin biosynthesis in saffron
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071, Albacete, Spain
    Plant Mol Biol 91:355-74. 2016
    ....
  9. doi request reprint The expression of a chromoplast-specific lycopene beta cyclase gene is involved in the high production of saffron's apocarotenoid precursors
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, E 02071 Albacete, Spain
    J Exp Bot 61:105-19. 2010
    ..goulimyi and C. cancellatus, the absence of transcripts suggests that transcriptional regulation of CstLcyB2a is responsible for the low apocarotenoid content in these species...
  10. ncbi request reprint Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens
    Aránzazu Palacín
    Departamento de Biotecnologia, Escuela Técnica Superior Ingenieros Agrónomos, Universidad Politecnica de Madrid, Madrid, Spain
    J Allergy Clin Immunol 117:1423-9. 2006
    ..Food IgE-mediated allergy to members of the Brassicaceae family has been increasingly reported...
  11. doi request reprint Crocins transport in Crocus sativus: the long road from a senescent stigma to a newborn corm
    Angela Rubio-Moraga
    ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, E 02071, Spain
    Phytochemistry 71:1506-13. 2010
    ....
  12. doi request reprint Genomic analysis and gene structure of the plant carotenoid dioxygenase 4 family: a deeper study in Crocus sativus and its allies
    Oussama Ahrazem
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Escuela Tecnica Superior de Ingenieros Agronomos, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Genomics 96:239-50. 2010
    ....
  13. ncbi request reprint Lipid transfer proteins and allergy to oranges
    Oussama Ahrazem
    Unidad de Bioquimica, Departamento de Biotecnologia, ETS Ingenieros Agronomos, UPM, Madrid, Spain
    Int Arch Allergy Immunol 137:201-10. 2005
    ..No citrus fruit allergen has been characterized to date. We sought to identify and isolate citrus fruit LTPs and to explore their relevance in orange allergy...
  14. doi request reprint Identification and possible role of a MYB transcription factor from saffron (Crocus sativus)
    Lourdes Gomez-Gomez
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Instituto Botánico, ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete 02071, Spain
    J Plant Physiol 169:509-15. 2012
    ..No transcripts were found in stigma tissues of Crocus species characterized by branched stigma morphology. Taken together, these results suggest that CsMYB1 may be involved in the regulation of stigma morphology in Crocus...
  15. pmc Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production
    José L Rambla
    Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La ManchaAlbacete, Spain Instituto de Biología Molecular y Celular de Plantas, CSIC Universidad Politécnica de ValenciaValencia, Spain
    Front Plant Sci 7:1619. 2016
    ..Correlation analysis showed a higher degree of overall correlation in precursor/volatile metabolite-metabolite levels in Airén, confirming the enriched aroma bouquet characteristic of the white varieties...
  16. doi request reprint New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus
    Angela Rubio-Moraga
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071, Albacete, Spain
    Plant Mol Biol 86:555-69. 2014
    ..Lutein, neoxanthin and violaxanthin levels in Nicotiana leaves were markedly reduced when CsCCD4c is over expressed, suggesting that CsCCD4c recognizes these carotenoids as substrates. ..
  17. doi request reprint Ectopic expression of a stress-inducible glycosyltransferase from saffron enhances salt and oxidative stress tolerance in Arabidopsis while alters anchor root formation
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain Fundación Parque Científico y Tecnológico de Albacete, Spain
    Plant Sci 234:60-73. 2015
    ....
  18. doi request reprint Saffron: Its Phytochemistry, Developmental Processes, and Biotechnological Prospects
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnologı́a Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    J Agric Food Chem 63:8751-64. 2015
    ....
  19. pmc Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach
    Oussama Ahrazem
    Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla La Mancha, Albacete, Spain
    PLoS ONE 11:e0168736. 2016
    ....
  20. pmc Unraveling Massive Crocins Transport and Accumulation through Proteome and Microscopy Tools during the Development of Saffron Stigma
    Lourdes Gomez-Gomez
    Botanical Institute, Department of Science Technology, Agroforestry and Genetics, Faculty of Pharmacy, University of Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Int J Mol Sci 18:. 2017
    ..and crocin (e.g., ζ-carotene desaturase (ZDS) and plastid-lipid-associated proteins (PLAP2)) accumulation; in addition, candidate aldehyde dehydrogenase (ADH) genes were highlighted...
  21. doi request reprint Screening for polyphenols, antioxidant and antimicrobial activitiesof extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in south-eastern Spain
    Angela Rubio-Moraga
    Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    J Ethnopharmacol 148:287-96. 2013
    ..Some of them are important medicinal plants used in several countries for many different purposes. However, studies addressing the phytochemistry of many of these species or their biological activities are currently nonexistent...
  22. pmc Saffron is a monomorphic species as revealed by RAPD, ISSR and microsatellite analyses
    Angela Rubio-Moraga
    Departamento de Ciencia y Tecnología Agroforestal y Genética, ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, 02071, Spain
    BMC Res Notes 2:189. 2009
    ..Since no molecular markers for this species have been found in the literature, the objective of this study was to determine whether phenotypical differences found in C. sativus were supported by molecular analyses...
  23. pmc Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation
    Angela Rubio Moraga
    Departamento de Ciencia y Tecnología Agroforestal y Genética, ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, Albacete, 02071, Spain
    BMC Plant Biol 9:109. 2009
    ..sativus, despite the importance of these compounds as antioxidant agents. Moreover, their bitter taste makes them excellent candidates for consideration as potential organoleptic agents of saffron spice, the dry stigmas of C. sativus...
  24. doi request reprint Metabolite and target transcript analyses during Crocus sativus stigma development
    Angela Rubio Moraga
    Departamento de Ciencia y Tecnología Agroforestal y Genética, ETSIA, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    Phytochemistry 70:1009-16. 2009
    ..Fatty acid derivates, which predominated at the earlier developmental stages, were observed at low levels in later stages...
  25. pmc Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting
    Angela Rubio-Moraga
    Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla La Mancha, Campus Universitario s n, 02071 Albacete, Spain
    BMC Plant Biol 14:171. 2014
    ..We focused on two of the genes within the SL pathway, CCD7 and CCD8, encoding carotenoid cleavage enzymes required for the production of SLs...
  26. pmc Molecular and immunological characterization of the glycosylated orange allergen Cit s 1
    Gerald Pöltl
    Department für Chemie, Universität für Bodenkultur, Muthgasse 18, A 1190 Wien, Austria
    Glycobiology 17:220-30. 2007
    ....
  27. ncbi request reprint Lipid transfer protein is involved in rhinoconjunctivitis and asthma produced by rice inhalation
    Ernesto Enrique
    J Allergy Clin Immunol 116:926-8. 2005