M Isabel González Siso

Summary

Country: Spain

Publications

  1. pmc New extremophilic lipases and esterases from metagenomics
    Olalla López-López
    University of A Coruna, Faculty of Sciences, Department of Cellular and Molecular Biology, Biochemistry and Molecular Biology Area Campus A Zapateira s n, 15071, A Coruna, Spain
    Curr Protein Pept Sci 15:445-55. 2014
  2. pmc Dealing with different methods for Kluyveromyces lactis beta-galactosidase purification
    M Becerra
    Departamento de Bioloxía Celular e Molecular Facultade de Ciencias Campus da Zapateira s n 15071 A Coruña Spain
    Biol Proced Online 1:48-58. 1998
  3. doi request reprint The yeast hypoxic responses, resources for new biotechnological opportunities
    M Isabel González Siso
    Departamento de Biologia Celular y Molecular, F Ciencias, Universidad de A Coruna, Coruna, Spain
    Biotechnol Lett 34:2161-73. 2012
  4. pmc Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
    Saul N Rocha
    Department of Chemical Engineering, Polytechnic School of Engineering, University of Sao Paulo, CP 61548, 05424 970 São Paulo SP, Brazil
    Microb Cell Fact 9:4. 2010
  5. pmc Sugar metabolism, redox balance and oxidative stress response in the respiratory yeast Kluyveromyces lactis
    M Isabel González-Siso
    Department of Molecular and Cell Biology, University of A Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    Microb Cell Fact 8:46. 2009
  6. ncbi request reprint Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media
    Manuel Becerra
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    J Biotechnol 109:131-7. 2004
  7. pmc Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger beta-galactosidase
    Angel Pereira Rodríguez
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira, s n 15071, A Coruna, Spain
    Microb Cell Fact 5:41. 2006
  8. pmc Crystallization and preliminary X-ray crystallographic analysis of beta-galactosidase from Kluyveromyces lactis
    Angel Pereira-Rodríguez
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:297-300. 2010
  9. doi request reprint Heterologous expression of an esterase from Thermus thermophilus HB27 in Saccharomyces cerevisiae
    Olalla López-López
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, 15071 A Coruna, Spain
    J Biotechnol 145:226-32. 2010
  10. ncbi request reprint An approach to the hypoxic and oxidative stress responses in Kluyveromyces lactis by analysis of mRNA levels
    Moises Blanco
    Department of Molecular and Cell Biology, University of A Coruna, A Coruna, Spain
    FEMS Yeast Res 7:702-14. 2007

Collaborators

  • M Esperanza Cerdán
  • M Isabel González Siso
  • Manuel Becerra
  • Olalla López-López
  • M Isabel González-Siso
  • Nuria Tarrío
  • María E Cerdán
  • Angel Pereira-Rodríguez
  • Saul N Rocha
  • Moises Blanco
  • Angel Pereira Rodríguez
  • Silvia M Díaz Prado
  • María I González Siso
  • Angel Vizoso Vázquez
  • Mónica Lamas Maceiras
  • Julia Sanz-Aparicio
  • Andreas K Gombert
  • Lorenzo Pastrana
  • María I González-Siso
  • Rafael Fernández-Leiro
  • Pablo Fuciños
  • M Luisa Rúa
  • José Abrahão-Neto
  • Ana García-Leiro
  • Elena Canto
  • Laura Núñez
  • M Cristina Trillo
  • M Becerra
  • Rafael Fernández Leiro
  • Esther Rodríguez-Belmonte
  • E Cerdan
  • M I González Siso

Detail Information

Publications12

  1. pmc New extremophilic lipases and esterases from metagenomics
    Olalla López-López
    University of A Coruna, Faculty of Sciences, Department of Cellular and Molecular Biology, Biochemistry and Molecular Biology Area Campus A Zapateira s n, 15071, A Coruna, Spain
    Curr Protein Pept Sci 15:445-55. 2014
    ..Here, we review the latest advances and findings from research into new extremophilic lipases and esterases, using metagenomic approaches, and their potential industrial and biotechnological applications. ..
  2. pmc Dealing with different methods for Kluyveromyces lactis beta-galactosidase purification
    M Becerra
    Departamento de Bioloxía Celular e Molecular Facultade de Ciencias Campus da Zapateira s n 15071 A Coruña Spain
    Biol Proced Online 1:48-58. 1998
    ..Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme...
  3. doi request reprint The yeast hypoxic responses, resources for new biotechnological opportunities
    M Isabel González Siso
    Departamento de Biologia Celular y Molecular, F Ciencias, Universidad de A Coruna, Coruna, Spain
    Biotechnol Lett 34:2161-73. 2012
    ..Possible pathways and target genes, which might be of particular interest for the improvement of biotechnological applications, are evaluated...
  4. pmc Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
    Saul N Rocha
    Department of Chemical Engineering, Polytechnic School of Engineering, University of Sao Paulo, CP 61548, 05424 970 São Paulo SP, Brazil
    Microb Cell Fact 9:4. 2010
    ..lactis CBS 2359 using three different expression systems. We aimed at verifying how each expression system would affect protein expression, secretion/localization, post-translational modification, and biochemical properties...
  5. pmc Sugar metabolism, redox balance and oxidative stress response in the respiratory yeast Kluyveromyces lactis
    M Isabel González-Siso
    Department of Molecular and Cell Biology, University of A Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    Microb Cell Fact 8:46. 2009
    ....
  6. ncbi request reprint Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media
    Manuel Becerra
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    J Biotechnol 109:131-7. 2004
    ..This strain is effective for the heterologous production and release of K. lactis beta-galactosidase into the extra-cellular medium after osmotic shock...
  7. pmc Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger beta-galactosidase
    Angel Pereira Rodríguez
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira, s n 15071, A Coruna, Spain
    Microb Cell Fact 5:41. 2006
    ..In both, a heterologous signal peptide for secretion was also included at the N-terminus of the recombinant proteins. One of the hybrid proteins obtained has interesting properties for its biotechnological utilization...
  8. pmc Crystallization and preliminary X-ray crystallographic analysis of beta-galactosidase from Kluyveromyces lactis
    Angel Pereira-Rodríguez
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:297-300. 2010
    ..Finally, the crystal quality was improved using the streak-seeding technique and a complete diffraction data set was collected at 2.8 A resolution...
  9. doi request reprint Heterologous expression of an esterase from Thermus thermophilus HB27 in Saccharomyces cerevisiae
    Olalla López-López
    Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruna, 15071 A Coruna, Spain
    J Biotechnol 145:226-32. 2010
    ..3h at 85 degrees C) was higher, optimum temperature (40 degrees C) was lower and optimum pH (7.5-8.5) was similar. These characteristics support potential biotechnological applications of the recombinant esterase...
  10. ncbi request reprint An approach to the hypoxic and oxidative stress responses in Kluyveromyces lactis by analysis of mRNA levels
    Moises Blanco
    Department of Molecular and Cell Biology, University of A Coruna, A Coruna, Spain
    FEMS Yeast Res 7:702-14. 2007
    ..lactis strains in which glycolysis is blocked at the phosphoglucose isomerase step. This approach is the first analysis carried out in K. lactis for the majority of the genes selected...
  11. ncbi request reprint Characterization of the second external alternative dehydrogenase from mitochondria of the respiratory yeast Kluyveromyces lactis
    Nuria Tarrío
    Department of Molecular and Cell Biology, University of A Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    Biochim Biophys Acta 1757:1476-84. 2006
    ..KlNde2p shows lower affinity for NADPH than KlNde1p. Both enzymes show similar pH optimum...
  12. ncbi request reprint Isolation and transcriptional regulation of the Kluyveromyces lactis FBA1 (fructose-1,6-bisphosphate aldolase) gene
    Silvia M Díaz Prado
    Department of Molecular and Cell Biology, University of A Coruna, Campus da Zapateira s n, 15071 A Coruna, Spain
    Can J Microbiol 50:645-52. 2004
    ..lactis and Saccharomyces cerevisiae, i.e., a respiratory vs. fermentative yeast...