Genomes and Genes
Affiliation: Center for Genomic Regulation
- B young againThomas Graf
Center for Genomic Regulation, Carrer Dr Aiguader 88, E 08003 Barcelona, Spain
Immunity 28:606-8. 2008..Hanna et al. (2008) report in a recent issue of Cell that a defined set of transcription factors can reprogram mature B cells back to pluripotent stem cells...
- Forcing cells to change lineagesThomas Graf
Center for Genomic Regulation and ICREA, 08003 Barcelona, Spain
Nature 462:587-94. 2009..The lessons learnt provide a rubric for how cells may be manipulated for therapeutic purposes...
- Heterogeneity of embryonic and adult stem cellsThomas Graf
Center for Genomic Regulation, ICREA and Pompeu Fabra University, 08003 Barcelona, Spain
Cell Stem Cell 3:480-3. 2008..These cell states show a bias in their differentiation potential and correlate with specific patterns of transcription factor expression and chromatin modifications...
- Historical origins of transdifferentiation and reprogrammingThomas Graf
ICREA Professor, Center for Genomic Regulation and Pompeu Fabra University, 08003 Barcelona, Spain
Cell Stem Cell 9:504-16. 2011..In this article, I give a historical and personal perspective of the events that set the stage for our current understanding of cellular reprogramming...
- C/EBPa-mediated activation of microRNAs 34a and 223 inhibits Lef1 expression to achieve efficient reprogramming into macrophagesJavier Rodriguez-Ubreva
Chromatin and Disease Group, Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, L Hospitalet de Llobregat, Barcelona, Spain
Mol Cell Biol 34:1145-57. 2014....
- HDAC7 is a repressor of myeloid genes whose downregulation is required for transdifferentiation of pre-B cells into macrophagesBruna Barneda-Zahonero
Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, Barcelona, Spain
PLoS Genet 9:e1003503. 2013..Thus, in B cells HDAC7 is a transcriptional repressor of undesirable genes. Our findings uncover a novel role for HDAC7 in maintaining the identity of a particular cell type by silencing lineage-inappropriate genes...
- CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with ageChristos Gekas
Gene Regulation, Stem Cells and Cancer Program, Centre for Genomic Regulation, Barcelona, Spain
Blood 121:4463-72. 2013..In summary, our data provide a novel marker to identify a myeloid-biased HSC subtype that becomes prevalent with age and highlights the dogma of HSC regulation by their progeny...
- CCAAT/enhancer binding protein alpha (C/EBP(alpha))-induced transdifferentiation of pre-B cells into macrophages involves no overt retrodifferentiationAlessandro di Tullio
Cancer and Differentiation Program, Center for Genomic Regulation and Pompeu Fabra University, 08003 Barcelona, Spain
Proc Natl Acad Sci U S A 108:17016-21. 2011..Together, our findings are consistent with the notion that the conversion from pre-B cells to macrophages is mostly direct and does not involve overt retrodifferentiation...
- C/EBPα induces highly efficient macrophage transdifferentiation of B lymphoma and leukemia cell lines and impairs their tumorigenicityFrancesca Rapino
Center for Genomic Regulation, Universidad Pompeu Fabra and Institució Catalana de Recerca i Estudis Avançats, Dr Aiguader 88, 08003 Barcelona, Spain
Cell Rep 3:1153-63. 2013....
- Tet2 facilitates the derepression of myeloid target genes during CEBPα-induced transdifferentiation of pre-B cellsEric M Kallin
Gene Regulation, Stem Cells and Cancer Program, Center for Genomic Regulation, and Pompeu Fabra University, Barcelona, Spain
Mol Cell 48:266-76. 2012..Activation of these target genes was accompanied by rapid increases of promoter hydroxy-methylation. Our observations indicate that Tet2 helps CEBPα rapidly derepress myeloid genes during the conversion of pre-B cells into macrophages...
- Fibroblast-derived induced pluripotent stem cells show no common retroviral vector insertionsFlorencio Varas
Differentiation and Cancer, Center for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain
Stem Cells 27:300-6. 2009..We conclude that Oct4, Sox2, Klf4, and c-Myc are sufficient to promote fibroblast-to-iPS cell reprogramming and propose that the observed low reprogramming frequencies may have alternative explanations...
- C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cellsBruno Di Stefano
1 Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation CRG, Dr Aiguader 88, 08003 Barcelona, Spain 2 Universitat Pompeu Fabra UPF, Dr Aiguader 88, 08003 Barcelona, Spain
Nature 506:235-9. 2014..The rapid iPS reprogramming approach described here should help to fully elucidate the process and has potential clinical applications. ..
- Induced pluripotent stem cell-derived human platelets: one step closer to the clinicChristos Gekas
Differentiation and Cancer Program, Center for Genomic Regulation, 08003 Barcelona, Spain
J Exp Med 207:2781-4. 2010..Notably, their lack of nucleus renders platelets unable to retain the pluripotent or tumorigenic properties of iPS cells...
- Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changesJavier Rodriguez-Ubreva
Chromatin and Disease Group, Cancer Epigenetics and Biology Programme PEBC, Bellvitge Biomedical Research Institute IDIBELL, 08907 L Hospitalet de Llobregat, Barcelona, Spain
Nucleic Acids Res 40:1954-68. 2012....
- Zrf1 is required to establish and maintain neural progenitor identityLuigi Aloia
Centre for Genomic Regulation CRG, Universitat Pompeu Fabra, 08003 Barcelona, Spain
Genes Dev 28:182-97. 2014..Depletion of Zrf1 in vivo impairs the expression of key self-renewal regulators and Wnt ligand genes in RGCs. Thus, we demonstrate that Zrf1 plays an essential role in NPC generation and maintenance. ..
- Musashi 2 is a regulator of the HSC compartment identified by a retroviral insertion screen and knockout miceLuisa de Andres-Aguayo
Differentiation and Cancer Program, Center for Genomic Regulation and UPF, Barcelona, Spain
Blood 118:554-64. 2011..Our data indicate that Msi2 maintains the stem cell compartment mainly by regulating the proliferation of primitive progenitors downstream of LT-HSCs...
- C/EBPα bypasses cell cycle-dependency during immune cell transdifferentiationAlessandro di Tullio
Gene Regulation, Stem Cells and Cancer Program, Center for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain
Cell Cycle 11:2739-46. 2012....
- Hi-TEC reprogramming for organ regenerationBruno Di Stefano
Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation and Universitat Pompeu Fabra, Dr Aiguader 88, 08003 Barcelona, Spain
Nat Cell Biol 16:824-5. 2014..Thymic epithelial cells that were obtained from fibroblasts by Foxn1 overexpression support the formation of an ectopic thymus following transplantation. ..
- A new path to leukemia with WITJose Luis Sardina
Center for Genomic Regulation, c Dr Aiguader, 88, PRBB Building, 08003 Barcelona, Spain Pompeu Fabra University, Plaça de la Mercè, 10, 08002 Barcelona, Spain Electronic address
Mol Cell 57:573-4. 2015..This pathway consists of WT1, IDH1/2, and TET2 (WIT) genes, with exclusive mutations of the three genes inducing myeloid cell proliferation...
- Determinants of lymphoid-myeloid lineage diversificationCatherine V Laiosa
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Annu Rev Immunol 24:705-38. 2006....
- Stepwise reprogramming of B cells into macrophagesHuafeng Xie
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine Cancer Research Center, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Cell 117:663-76. 2004..Our observations indicate that C/EBPalpha and beta remodel the transcription network of B cells into that of macrophages through a series of parallel and sequential changes that require endogenous PU.1...
- PU.1 and C/EBPalpha/beta convert fibroblasts into macrophage-like cellsRu Feng
Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA
Proc Natl Acad Sci U S A 105:6057-62. 2008..Our data suggest that it might become possible to induce the transdifferentiation of skin-derived fibroblasts into cell types desirable for tissue regeneration...
- Making eosinophils through subtle shifts in transcription factor expressionKelly McNagny
The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
J Exp Med 195:F43-7. 2002
- Myeloid or lymphoid promiscuity as a critical step in hematopoietic lineage commitmentToshihiro Miyamoto
Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
Dev Cell 3:137-47. 2002..Thus, the accessibility for multiple myeloid or lymphoid programs promiscuously may allow flexibility in fate commitments at these multipotent stages...
- Distinguishable live erythroid and myeloid cells in beta-globin ECFP x lysozyme EGFP miceSusanne Heck
Department of Molecular and Developmental Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Blood 101:903-6. 2003..The new mouse lines should become useful tools to dissect the branching between erythroid and myelomonocytic cells during in vitro differentiation of definitive multipotent progenitors...
- E26 leukemia virus converts primitive erythroid cells into cycling multilineage progenitorsKelly M McNagny
Biomedical Research Centre, Department of Medical Genetics, University of British Columbia, Vancouver, Canada
Blood 101:1103-10. 2003..These results suggest that the Myb-Ets oncoprotein of the E26 leukemia virus converts primitive erythroid cells into proliferating definitive-type multipotent hematopoietic progenitors...
- Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycanTomas Ganz
Department of Medicine, David Geffen School of Medicine, Los Angeles, CA 90095 1690, USA
Blood 101:2388-92. 2003..Our data indicate that tissue injury is normally limited by prompt degradation of bacterial macromolecules that trigger innate immunity and inflammation...
- MafB deficiency causes defective respiratory rhythmogenesis and fatal central apnea at birthBruno Blanchi
Centre d immunologie de Marseille Luminy, CNRS INSERM Université Mediterrané, Campus de Luminy, Case 906, 13288 Marseille Cedex 09, France
Nat Neurosci 6:1091-100. 2003..Our results show an essential role of MafB in central respiratory control, possibly involving the specification of rhythmogenic preBötC neurons...
- A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumorsJeffrey Wyckoff
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Cancer Res 64:7022-9. 2004..This work provides the first direct evidence for a synergistic interaction between macrophages and tumor cells during cell migration in vivo and indicates a mechanism for how macrophages may contribute to metastasis...
- Fluorescent protein-cell labeling and its application in time-lapse analysis of hematopoietic differentiationMatthias Stadtfeld
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA
Methods Mol Med 105:395-412. 2005..Finally, we make recommendations about which of these filters to choose when working with specific fluorescent proteins...
- PU.1 is not strictly required for B cell development and its absence induces a B-2 to B-1 cell switchMin Ye
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
J Exp Med 202:1411-22. 2005..Furthermore, we found that B-2 cells express substantially more PU.1 than B-1 cells, which is consistent with the idea that maintenance of the B-2 cell phenotype requires relatively high levels of PU.1, but B-1 cells require little...
- Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesisHarald Schulze
Dana Farber Cancer Institute, One Jimmy Fund Way, Boston, MA 02115, USA
Blood 107:3868-75. 2006..These observations collectively suggest a signaling pathway wherein PI-4,5-P(2) might facilitate DMS development and local assembly of actin fibers in preparation for platelet biogenesis...
- Reprogramming of committed T cell progenitors to macrophages and dendritic cells by C/EBP alpha and PU.1 transcription factorsCatherine V Laiosa
Department of Developmental and Molecular Biology and the Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Immunity 25:731-44. 2006..1 in multilineage precursors...
- Klf2 is an essential regulator of vascular hemodynamic forces in vivoJohn S Lee
Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Dev Cell 11:845-57. 2006..These findings identify Klf2 as an essential hemodynamic regulator in vivo and suggest that hemodynamic regulation in response to fluid shear stress is required for cardiovascular development and function...
- Early decisions in lymphoid developmentMin Ye
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, New York Bronx, NY 10461, USA
Curr Opin Immunol 19:123-8. 2007..In conclusion, although a lot is known about B and T cell commitment, more work needs to be done to clarify the earliest steps in lymphoid specification...
- CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulationJinghang Zhang
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA
Exp Hematol 35:490-499. 2007..Development of a mouse line permitting live imaging of cells expressing CD41/GpIIb as a means to study megakaryopoiesis...
- Reciprocal activation of GATA-1 and PU.1 marks initial specification of hematopoietic stem cells into myeloerythroid and myelolymphoid lineagesYojiro Arinobu
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Cell Stem Cell 1:416-27. 2007..1 primarily organizes the hematopoietic lineage fate decision to form the earliest hematopoietic branchpoint that comprises isolatable myeloerythroid and myelolymphoid progenitor populations...
- Immunology: blood lines redrawnThomas Graf
Nature 452:702-3. 2008
- Differentiation plasticity of hematopoietic cellsThomas Graf
Albert Einstein College of Medicine, Bronx, NY, USA
Blood 99:3089-101. 2002