Paul D van Helden

Summary

Affiliation: University of Stellenbosch
Country: South Africa

Publications

  1. ncbi request reprint Molecular epidemiology of TB: challenging dogmas and asking new questions
    Paul D van Helden
    US MRC Centre for Molecular and Cellular Biology and Department of Medical Biochemistry, Stellenbosch University, Tygerberg, South Africa
    IUBMB Life 53:219-23. 2002
  2. ncbi request reprint Drug resistance in Mycobacterium tuberculosis
    Rabia Johnson
    Division of Molecular Biology and Human Genetics, Department of Biomedical Science, Faculty of Health Science, Tygerberg, Stellenbosch University, South Africa
    Curr Issues Mol Biol 8:97-111. 2006
  3. pmc Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania
    Erasto V Mbugi
    Department of Biochemistry, Muhimbili University of Health and Allied Sciences, P O Box 65001 Dar es Salaam, Tanzania Departments of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, P O Box 65001 Dar es Salaam, Tanzania Electronic address
    Tuberculosis (Edinb) 95:170-8. 2015
  4. pmc Prevalence and risk factors for infection of bovine tuberculosis in indigenous cattle in the Serengeti ecosystem, Tanzania
    Bugwesa Z Katale
    Department of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences MUHAS, P, O BOX 65001, Dar es Salaam, Tanzania
    BMC Vet Res 9:267. 2013
  5. pmc Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens
    Hendrik J Nel
    Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, NRF DST Centre of Excellence in Biomedical TB Research, Faculty Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
    BMC Microbiol 14:9. 2014
  6. pmc Using multi-way admixture mapping to elucidate TB susceptibility in the South African Coloured population
    Michelle Daya
    Molecular Biology and Human Genetics, MRC Centre for TB Research and the DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
    BMC Genomics 15:1021. 2014
  7. pmc A threshold value for the time delay to TB diagnosis
    Pieter W Uys
    Division of Molecular Biology and Human Genetics, MRC Center for Molecular and Cellular Biology, Faculty of Health Sciences, Stellenbosch University, Cape Town, Western Cape, Republic of South Africa
    PLoS ONE 2:e757. 2007
  8. pmc High yield of culture-based diagnosis in a TB-endemic setting
    Anne Marie Demers
    Department of Medicine, McGill University, Montreal, Quebec, Canada
    BMC Infect Dis 12:218. 2012
  9. doi request reprint A new TB vaccine: fact or fiction?
    Paul D van Helden
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa
    Comp Immunol Microbiol Infect Dis 36:287-94. 2013
  10. pmc Bovine tuberculosis in African buffaloes: observations regarding Mycobacterium bovis shedding into water and exposure to environmental mycobacteria
    Anita L Michel
    Tuberculosis Laboratory, ARC Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa
    BMC Vet Res 3:23. 2007

Detail Information

Publications105 found, 100 shown here

  1. ncbi request reprint Molecular epidemiology of TB: challenging dogmas and asking new questions
    Paul D van Helden
    US MRC Centre for Molecular and Cellular Biology and Department of Medical Biochemistry, Stellenbosch University, Tygerberg, South Africa
    IUBMB Life 53:219-23. 2002
    ..These findings alone demand urgent attention and the design of optimal intervention strategies to reduce the burden of disease...
  2. ncbi request reprint Drug resistance in Mycobacterium tuberculosis
    Rabia Johnson
    Division of Molecular Biology and Human Genetics, Department of Biomedical Science, Faculty of Health Science, Tygerberg, Stellenbosch University, South Africa
    Curr Issues Mol Biol 8:97-111. 2006
    ....
  3. pmc Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania
    Erasto V Mbugi
    Department of Biochemistry, Muhimbili University of Health and Allied Sciences, P O Box 65001 Dar es Salaam, Tanzania Departments of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, P O Box 65001 Dar es Salaam, Tanzania Electronic address
    Tuberculosis (Edinb) 95:170-8. 2015
    ..Other types found in lower prevalence are Beijing, Haarlem, X, S and MANU. The Haarlem, EAI_Somalia, LAM3 and S/convergent and X2 subfamilies found in this study were not reported in previous studies in Tanzania. ..
  4. pmc Prevalence and risk factors for infection of bovine tuberculosis in indigenous cattle in the Serengeti ecosystem, Tanzania
    Bugwesa Z Katale
    Department of Microbiology and Immunology, School of Medicine, Muhimbili University of Health and Allied Sciences MUHAS, P, O BOX 65001, Dar es Salaam, Tanzania
    BMC Vet Res 9:267. 2013
    ..This study estimated the prevalence and risk factors associated with bovine tuberculosis transmission in indigenous cattle at the human-animal interface in the Serengeti ecosystem of Tanzania...
  5. pmc Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens
    Hendrik J Nel
    Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, NRF DST Centre of Excellence in Biomedical TB Research, Faculty Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
    BMC Microbiol 14:9. 2014
    ..bovis) BCG, would alter host immune responses to, or the pathological effect of, either infection...
  6. pmc Using multi-way admixture mapping to elucidate TB susceptibility in the South African Coloured population
    Michelle Daya
    Molecular Biology and Human Genetics, MRC Centre for TB Research and the DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
    BMC Genomics 15:1021. 2014
    ..In this study, we infer local ancestry using the novel algorithm implemented in RFMix, with the emphasis on identifying regions of excess San or Bantu ancestry, which we hypothesize may harbour TB susceptibility genes...
  7. pmc A threshold value for the time delay to TB diagnosis
    Pieter W Uys
    Division of Molecular Biology and Human Genetics, MRC Center for Molecular and Cellular Biology, Faculty of Health Sciences, Stellenbosch University, Cape Town, Western Cape, Republic of South Africa
    PLoS ONE 2:e757. 2007
    ..It is plausible that the typical long delay from the onset of infectious disease to diagnosis and commencement of treatment is almost certainly the major factor contributing to the high rate of transmission...
  8. pmc High yield of culture-based diagnosis in a TB-endemic setting
    Anne Marie Demers
    Department of Medicine, McGill University, Montreal, Quebec, Canada
    BMC Infect Dis 12:218. 2012
    ..In most of the world, microbiologic diagnosis of tuberculosis (TB) is limited to microscopy. Recent guidelines recommend culture-based diagnosis where feasible...
  9. doi request reprint A new TB vaccine: fact or fiction?
    Paul D van Helden
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa
    Comp Immunol Microbiol Infect Dis 36:287-94. 2013
    ..We present different aspects of the development of vaccines against TB, outline some complications and suggest some new ways to consider this problem...
  10. pmc Bovine tuberculosis in African buffaloes: observations regarding Mycobacterium bovis shedding into water and exposure to environmental mycobacteria
    Anita L Michel
    Tuberculosis Laboratory, ARC Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa
    BMC Vet Res 3:23. 2007
    ..bovis. Hence, all study buffaloes in the vaccination trial could be considered potential shedders and provided a suitable setting for investigating questions relating to the tenacity of M. bovis shed in water...
  11. pmc Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis
    Catriona J Harper
    DST NRF Centre of Excellence for Biomedical TB Research, Department of Molecular Biology and Human Genetics, Faculty of Health Sciences, University of Stellenbosch, Tygerberg, South Africa
    BMC Microbiol 10:138. 2010
    ....
  12. pmc Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology
    Karine Brudey
    Unite de la Tuberculose et des Mycobacteries, Institut Pasteur de Guadeloupe, Guadeloupe
    BMC Microbiol 6:23. 2006
    ....
  13. pmc The non-clonality of drug resistance in Beijing-genotype isolates of Mycobacterium tuberculosis from the Western Cape of South Africa
    Thomas R Ioerger
    Department of Computer Science and Engineering, Texas A and M University, College Station, TX, USA
    BMC Genomics 11:670. 2010
    ..Although the outbreak is considered to be due to clonal transmission, the relationship among drug resistant isolates has not yet been established...
  14. pmc Rainfall-driven sex-ratio genes in African buffalo suggested by correlations between Y-chromosomal haplotype frequencies and foetal sex ratio
    Pim van Hooft
    Resource Ecology Group, Wageningen University, 6708 PB Wageningen, The Netherlands
    BMC Evol Biol 10:106. 2010
    ..These sex-ratio (SR) genes modify (suppress) gamete transmission in their own favour at a fitness cost, allowing for stable polymorphism...
  15. pmc The economic divide and tuberculosis. Tuberculosis is not just a medical problem, but also a problem of social inequality and poverty
    Paul D van Helden
    MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, University of Stellenbosch, South Africa
    EMBO Rep 4:S24-8. 2003
  16. ncbi request reprint Antimicrobial resistance in tuberculosis: an international perspective
    Paul D van Helden
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg 7505, South Africa
    Expert Rev Anti Infect Ther 4:759-66. 2006
    ..We highlight some of the controversies and new findings in this area, as well as future perspectives requiring more active interventions, in addition to new technology and developments...
  17. ncbi request reprint Pulmonary infection due to Mycobacterium goodii in a spotted hyena (Crocuta crocuta) from South Africa
    Paul D van Helden
    DST NRF Centre of Excellence for Biomedical TB Research Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences Stellenbosch University, Tygerberg, South Africa
    J Wildl Dis 44:151-4. 2008
    ..goodii, a recently described fast-growing organism closely related to the nonpathogenic mycobacterial species M. smegmatis. The current study illustrates that this organism can be pathogenic and cause extensive pulmonary disease...
  18. ncbi request reprint 'Emerging' mycobacteria in South Africa
    P D van Helden
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505 South Africa
    J S Afr Vet Assoc 80:210-4. 2009
    ..Each case must be evaluated carefully with respect to the animal species involved, the environment in which the host is found and the mycobacterial species, and operational decisions made accordingly...
  19. ncbi request reprint Rate of reinfection tuberculosis after successful treatment is higher than rate of new tuberculosis
    Suzanne Verver
    Desmond Tutu TB Center, Department of Pediatrics and Child Health, Stellenbosch University, Cape Town, South Africa
    Am J Respir Crit Care Med 171:1430-5. 2005
    ..In a high-tuberculosis (TB) incidence area of Cape Town, South Africa, there is a very high rate of unexplained recurrent TB. The incidence of new bacteriologically confirmed disease in the area is 313 per 100,000 individuals...
  20. ncbi request reprint Patients with active tuberculosis often have different strains in the same sputum specimen
    Robin M Warren
    Medical Research Council Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Stellenbosch University, Tygerberg, South Africa
    Am J Respir Crit Care Med 169:610-4. 2004
    ..This finding could influence our understanding of the epidemiology of disease in high-incidence regions and our understanding for vaccine development...
  21. pmc Evidence for a rapid rate of molecular evolution at the hypervariable and immunogenic Mycobacterium tuberculosis PPE38 gene region
    Christopher R E McEvoy
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    BMC Evol Biol 9:237. 2009
    ..tuberculosis clinical isolates, other MTBC members and non tuberculous mycobacteria (NTM). Where possible we have extended this analysis to include the adjacent plcABC and PPE39/40 genomic regions...
  22. pmc Molecular characteristics and global spread of Mycobacterium tuberculosis with a western cape F11 genotype
    Thomas C Victor
    MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, University of Stellenbosch, Tygerberg 7505, South Africa
    J Clin Microbiol 42:769-72. 2004
    ..Careful study of dominant compared to rare strains should provide clues to their success and possibly provide new ideas for combating TB...
  23. doi request reprint Mutations in the rrs A1401G gene and phenotypic resistance to amikacin and capreomycin in Mycobacterium tuberculosis
    Frederick A Sirgel
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Science, Stellenbosch University, Cape Town, South Africa
    Microb Drug Resist 18:193-7. 2012
    ..Agar-based CC testing was found to be unreliable for resistance recognition of CAP in particular...
  24. ncbi request reprint Allelic variation in BTNL2 and susceptibility to tuberculosis in a South African population
    Marlo Möller
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and the DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, P O Box 19063, Stellenbosch University, Tygerberg 7505, South Africa
    Microbes Infect 9:522-8. 2007
    ....
  25. pmc A functional haplotype in the 3'untranslated region of TNFRSF1B is associated with tuberculosis in two African populations
    Marlo Möller
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and DST NRF Centre of Excellence for Biomedical TB Research, Stellenbosch University, Stellenbosch, South Africa
    Am J Respir Crit Care Med 181:388-93. 2010
    ..The pleiotropic cytokine tumor necrosis factor-alpha is essential to control tuberculosis infection, and various tumor necrosis factor family members and their respective receptors may contribute to tuberculosis risk...
  26. doi request reprint Investigation of chromosome 17 candidate genes in susceptibility to TB in a South African population
    Marlo Möller
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and the DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    Tuberculosis (Edinb) 89:189-94. 2009
    ..This study presents evidence that haplotypes in the NOS2A promoter influence susceptibility to TB and confirms the importance of NO production in the disease...
  27. pmc Polymorphisms in MC3R promoter and CTSZ 3'UTR are associated with tuberculosis susceptibility
    Lindsey A Adams
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre of Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    Eur J Hum Genet 19:676-81. 2011
    ..0004 and < 0.0001, respectively), indicating that pathways involving these proteins, not previously researched in this disease, could yield novel therapies for tuberculosis...
  28. ncbi request reprint Allele frequencies for glutathione S-transferase and N-acetyltransferase 2 differ in African population groups and may be associated with oesophageal cancer or tuberculosis incidence
    Craig H Adams
    Department of Medical Biochemistry, MRC Centre for Molecular and Cellular Biology, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    Clin Chem Lab Med 41:600-5. 2003
    ..In addition, the high proportion of NAT2 "fast" alleles may partially explain the high tuberculosis prevalence in South Africans, due to reduced isoniazid efficacy in the presence of rapid acetylation...
  29. ncbi request reprint Proportion of tuberculosis transmission that takes place in households in a high-incidence area
    Suzanne Verver
    Department of Paediatrics and Child Health, Stellenbosch University, Cape Town, South Africa
    Lancet 363:212-4. 2004
    ..The proportion of transmission in the community that took place in the household was 19%, and therefore, in this high-incidence area, tuberculosis transmission occurs mainly outside the household...
  30. pmc Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis
    Madalene Richardson
    MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    J Clin Microbiol 42:1302-4. 2004
    ..9% changed). Characteristic PGRS-restriction fragment length polymorphism motifs for different strain groupings may indicate distant evolutionary events leading to the differentiation of M. tuberculosis strain lineages...
  31. pmc Programmatically selected multidrug-resistant strains drive the emergence of extensively drug-resistant tuberculosis in South Africa
    Borna Muller
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland
    PLoS ONE 8:e70919. 2013
    ..Since 2010, ethambutol has been replaced by cycloserine or terizidone. The effect of standardized treatment on the acquisition of XDR-TB is not currently known...
  32. pmc Associations between human leukocyte antigen class I variants and the Mycobacterium tuberculosis subtypes causing disease
    Muneeb Salie
    MRC Centre for Molecular and Cellular Biology and the DST NRF Centre of Excellence for Biomedical TB Research, Division of Molecular Biology and Human Genetics, Stellenbosch University, Tygerberg
    J Infect Dis 209:216-23. 2014
    ..tuberculosis strains to specific human populations. Here we investigate the role of the human leukocyte antigen (HLA) class I genes in this biological process...
  33. doi request reprint embB306 mutations as molecular indicators to predict ethambutol susceptibility in Mycobacterium tuberculosis
    Frederick A Sirgel
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Science, Stellenbosch University, Cape Town, South Africa
    Chemotherapy 58:358-63. 2012
    ..Discordant results in conventional susceptibility testing of ethambutol against Mycobacterium tuberculosis may lead to underreporting of drug resistance...
  34. doi request reprint Infection of African buffalo (Syncerus caffer) by oryx bacillus, a rare member of the antelope clade of the Mycobacterium tuberculosis complex
    Nicolaas C Gey van Pittius
    Department of Science and Technology National Research Foundation Centre of Excellence for Biomedical Tuberculosis Research Medical Research Council Centre for Molecular and Cellular Biology Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505, South Africa
    J Wildl Dis 48:849-57. 2012
    ..tuberculosis complex species M. bovis in some African buffalo populations in South Africa...
  35. pmc Comparative analysis of Mycobacterium tuberculosis pe and ppe genes reveals high sequence variation and an apparent absence of selective constraints
    Christopher R E McEvoy
    Department of Science and Technology, Medical Research Council Centre for Molecular and Cellular Biology, Stellenbosch University, Tygerberg, Cape Town, South Africa
    PLoS ONE 7:e30593. 2012
    ..Since pe/ppe proteins are highly antigenic and have been studied as potential vaccine components these results should also prove informative for aspects of M. tuberculosis vaccine design...
  36. pmc Evolution and expansion of the Mycobacterium tuberculosis PE and PPE multigene families and their association with the duplication of the ESAT-6 (esx) gene cluster regions
    Nicolaas C Gey van Pittius
    DST NRF Centre of Excellence in Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    BMC Evol Biol 6:95. 2006
    ....
  37. doi request reprint Detection of Mycobacterium kansasii infection in a rhesus macaque (Macaca mulatta) using a modified QuantiFERON-TB Gold assay
    Sven D C Parsons
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg 7505, South Africa
    Vet Immunol Immunopathol 136:330-4. 2010
    ..However, these findings highlight the limitations of the QFG-IT to specifically detect infection by the pathogens M. tuberculosis and Mycobacterium bovis...
  38. ncbi request reprint Transmission of tuberculosis in a high incidence urban community in South Africa
    Suzanne Verver
    Department of Paediatrics and Child Health, Stellenbosch University, South Africa
    Int J Epidemiol 33:351-7. 2004
    ..The objective of this study was to identify risk factors for ongoing community transmission of tuberculosis (TB) in two densely populated urban communities with a high incidence rate of TB in Cape Town, South Africa...
  39. pmc A Subgroup of Latently Mycobacterium tuberculosis Infected Individuals Is Characterized by Consistently Elevated IgA Responses to Several Mycobacterial Antigens
    Ralf Baumann
    DST NRF Centre of Excellence for Biomedical TB Research and MRC Centre for TB Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg 7505, South Africa Lionex Diagnostics and Therapeutics, Salzdahlumer Straße 196, 38126 Braunschweig, Germany
    Mediators Inflamm 2015:364758. 2015
    ..The novel antigen NarL may also be promising for the antibody-based diagnosis of active TB cases. ..
  40. pmc Population structure of mixed Mycobacterium tuberculosis infection is strain genotype and culture medium dependent
    Madeleine Hanekom
    Division of Anatomy and Histology, Faculty of Health Sciences, Tygerberg, Stellenbosch University, Stellenbosch, Western Cape, South Africa
    PLoS ONE 8:e70178. 2013
    ..Molecular genotyping methods have shown infection with more than one Mycobacterium tuberculosis strain genotype in a single sputum culture, indicating mixed infection...
  41. doi request reprint Increased frequency of myeloid-derived suppressor cells during active tuberculosis and after recent mycobacterium tuberculosis infection suppresses T-cell function
    Nelita du Plessis
    1 Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, DST NRF Centre of Excellence for Biomedical TB Research, and
    Am J Respir Crit Care Med 188:724-32. 2013
    ..Inadequacy of T-cell responses may result in the development of tuberculosis (TB). Myeloid-derived suppressor cells (MDSCs) have been described as suppressors of T-cell function in cancer biology and recently in several infectious diseases...
  42. pmc The contraceptive depot medroxyprogesterone acetate impairs mycobacterial control and inhibits cytokine secretion in mice infected with Mycobacterium tuberculosis
    Léanie Kleynhans
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa
    Infect Immun 81:1234-44. 2013
    ..This could have major implications for the contraceptive policies not only in developing countries like South Africa but also worldwide...
  43. doi request reprint Emergence and treatment of multidrug resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in South Africa
    Elizabeth M Streicher
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, South Africa
    Infect Genet Evol 12:686-94. 2012
    ..An important step towards for curbing this epidemic will be collaboration between clinicians, laboratories and researchers to establish scientific knowledge and medical expertise to more efficiently guide public health policy...
  44. doi request reprint gyrA mutations and phenotypic susceptibility levels to ofloxacin and moxifloxacin in clinical isolates of Mycobacterium tuberculosis
    Frederick A Sirgel
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Science, Stellenbosch University, Stellenbosch, South Africa
    J Antimicrob Chemother 67:1088-93. 2012
    ..To compare mutations in the quinolone resistance-determining region of the gyrA gene and flanking sequences with the MICs of ofloxacin and moxifloxacin for Mycobacterium tuberculosis...
  45. pmc Analysis of eight genes modulating interferon gamma and human genetic susceptibility to tuberculosis: a case-control association study
    Marlo Möller
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, Faculty of Health Sciences, PO Box 19063, Stellenbosch University, Tygerberg 7505, South Africa
    BMC Infect Dis 10:154. 2010
    ..tuberculosis. Based on the hypothesis that genes which regulate interferon gamma may influence tuberculosis susceptibility, we investigated polymorphisms in eight candidate genes...
  46. ncbi request reprint Pulmonary infection due to the dassie bacillus (Mycobacterium tuberculosis complex sp.) in a free-living dassie (rock hyrax-Procavia capensis) from South Africa
    Sven Parsons
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, South Africa
    Tuberculosis (Edinb) 88:80-3. 2008
    ..The introduction of South African dassies into novel environments should be considered in this light...
  47. doi request reprint Insertion element IS6110-based restriction fragment length polymorphism genotyping of Mycobacterium tuberculosis
    Robin M Warren
    Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, DST NRF Centre of Excellence in Biomedical Tuberculosis Research, Stellenbosch University, PO Box 19063, Tygerberg, South Africa
    Methods Mol Biol 465:353-70. 2009
    ..In this chapter, the method of IS6110 DNA fingerprinting is explained in such a way that it can be easily duplicated by molecular epidemiologists and will give reproducible results...
  48. pmc Potential of rapid diagnosis for controlling drug-susceptible and drug-resistant tuberculosis in communities where Mycobacterium tuberculosis infections are highly prevalent
    Pieter W Uys
    Faculty of Health Sciences, MRC Center for Molecular and Cellular Biology, DST NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Tygerberg, South Africa
    J Clin Microbiol 47:1484-90. 2009
    ..The simulations showed that the rapid diagnosis of drug resistance can be expected to reduce the incidence of drug-resistant cases provided the additional measure of screening within the community is implemented...
  49. ncbi request reprint TB epidemiology and human genetics
    Paul D van Helden
    Department of Medical Biochemistry, US MRC Centre for Molecular and Cellular Biology, Faculty of Health Sciences, University of Stellenbosch, PO Box 19063, Tygerberg 7505, South Africa
    Novartis Found Symp 279:17-31; discussion 31-41, 216-9. 2006
    ..With adaptive immunity being of little value and good TB control programmes being rare, innate resistance is still our best defence against this..
  50. pmc The rationale for using rifabutin in the treatment of MDR and XDR tuberculosis outbreaks
    Frederick A Sirgel
    DST NRF Centre of Excellence for Biomedical TB Research, Division of Molecular Biology and Human Genetics, Faculty of Health Science, Stellenbosch University, Stellenbosch, South Africa
    PLoS ONE 8:e59414. 2013
    ..The use of RFB in the ECP may improve therapeutic success and consequently minimise the risk of ongoing transmission of drug resistant M. tuberculosis strains...
  51. doi request reprint Serodiagnostic markers for the prediction of the outcome of intensive phase tuberculosis therapy
    Ralf Baumann
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg 7505, South Africa
    Tuberculosis (Edinb) 93:239-45. 2013
    ..This approach holds promise and requires further evaluation for its utility in the prediction of treatment failure and relapse, the evaluation of new TB therapeutics, as well as in the care of individual patients...
  52. doi request reprint Development of a diagnostic gene expression assay for tuberculosis and its use under field conditions in African buffaloes (Syncerus caffer)
    Sven D C Parsons
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology Division of Molecular Biology and Human Genetics, Stellenbosch University, P O Box 19063, Tygerberg, 7505, South Africa
    Vet Immunol Immunopathol 148:337-42. 2012
    ..I.: 56-94%) and a specificity of 95% (95% C.I.: 75-100%). Further optimization of this assay could provide a highly useful tool for the diagnosis of MTC infection in exotic species...
  53. pmc Xpert MTB/RIF for rapid diagnosis of tuberculous lymphadenitis from fine-needle-aspiration biopsy specimens
    Louis J Ligthelm
    Division of Anatomical Pathology, National Health Laboratory Service, Tygerberg Hospital, Tygerberg, Cape Town, South Africa
    J Clin Microbiol 49:3967-70. 2011
    ..7% (95% confidence interval [CI], 86.6 to 100%) and 88.9% (95% CI, 69.6 to 100%), respectively, and it correctly identified 6/6 (100%) of the cytology smear-negative/culture-positive cases and 1 of 2 (50%) rifampin-resistant cases...
  54. pmc Rifampicin reduces susceptibility to ofloxacin in rifampicin-resistant Mycobacterium tuberculosis through efflux
    Gail E Louw
    Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, Cape Town, South Africa
    Am J Respir Crit Care Med 184:269-76. 2011
    ..Central dogma suggests that rifampicin resistance in Mycobacterium tuberculosis develops solely through rpoB gene mutations...
  55. ncbi request reprint The role of IS6110 in the evolution of Mycobacterium tuberculosis
    Christopher R E McEvoy
    DST NRF Centre of Excellence in Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    Tuberculosis (Edinb) 87:393-404. 2007
    ..tuberculosis and conclude that the presence of a moderate IS6110 copy number within the genome may provide the pathogen with a selective advantage that has aided its virulence...
  56. doi request reprint Serologic diagnosis of tuberculosis by combining Ig classes against selected mycobacterial targets
    Ralf Baumann
    DST NRF Centre of Excellence for Biomedical TB Research and MRC Centre for TB Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg 7505, South Africa Lionex Diagnostics and Therapeutics, 38126 Braunschweig, Germany
    J Infect 69:581-9. 2014
    ..Accurate, simple and cost-effective diagnostic tests are needed for diagnosis of active tuberculosis (TB). Serodiagnosis is attractive as it can be harnessed for point-of-care tests...
  57. doi request reprint Bovine TB in livestock and wildlife: what's in the genes?
    Nikki le Roex
    Department of Science and Technology National Research Foundation Centre of Excellence for Biomedical Tuberculosis Research Medical Research Council Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Stellenbosch University, Cape Town, South Africa
    Physiol Genomics 45:631-7. 2013
    ....
  58. pmc Zoonotic Mycobacterium bovis-induced tuberculosis in humans
    Borna Muller
    Faculty of Health Sciences, Stellenbosch University, Cape Town, South Africa
    Emerg Infect Dis 19:899-908. 2013
    ..These generally low incidence rates notwithstanding, available data indicated substantial consequences of this disease for some population groups and settings...
  59. pmc Potential of host markers produced by infection phase-dependent antigen-stimulated cells for the diagnosis of tuberculosis in a highly endemic area
    Novel N Chegou
    Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    PLoS ONE 7:e38501. 2012
    ..In this study, the levels of 11 host markers other than IFN-γ, were evaluated in whole blood culture supernatants after stimulation with M.tb infection phase-dependent antigens, for the diagnosis of TB disease...
  60. doi request reprint Modification of the QuantiFERON-TB Gold (In-Tube) assay for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer)
    Sven D C Parsons
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg 7505, South Africa
    Vet Immunol Immunopathol 142:113-8. 2011
    ..bovis was cultured from 21/26 slaughtered SICTT/mQFT-positive animals. The mQFT shows promise as a practical test for M. bovis infection in buffaloes and shows a sensitivity and specificity at least similar to that of the TST...
  61. pmc Population structure of multi- and extensively drug-resistant Mycobacterium tuberculosis strains in South Africa
    Violet N Chihota
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Stellenbosch, South Africa
    J Clin Microbiol 50:995-1002. 2012
    ..This situation may illustrate how failure of efficient health care delivery in one setting can burden health clinics in other areas...
  62. pmc Glutamine synthetase sequence evolution in the mycobacteria and their use as molecular markers for Actinobacteria speciation
    Don Hayward
    DST NRF Centre for Excellence in Biomedical Tuberculosis Research, US MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences Stellenbosch University, South Africa
    BMC Evol Biol 9:48. 2009
    ..Intriguingly, previous reports have shown that only one copy (glnA1) is essential for growth in M. tuberculosis, while the other copies (glnA2, glnA3 and glnA4) are not...
  63. ncbi request reprint High levels of intracellular IL-4 are expressed in circulating apoptotic T cells in patients with tuberculosis and in community controls
    Hanne Veenstra
    Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, Faculty of Health Sciences, University of Stellenbosch, P O Box 19063, Tygerberg 7505, South Africa
    Tuberculosis (Edinb) 88:21-30. 2008
    ..Cellular IL-4 production may represent a normal cellular growth factor mechanism which is disturbed at the onset of apoptosis...
  64. ncbi request reprint Host susceptibility to tuberculosis: CARD15 polymorphisms in a South African population
    Marlo Möller
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, PO Box 19063, Stellenbosch University, Tygerberg 7505, South Africa
    Mol Cell Probes 21:148-51. 2007
    ..We determined that the CD-associated mutations occur at very low frequencies in this population. Our results indicate that CARD15 is not a major susceptibility gene for TB in the South African Coloureds...
  65. doi request reprint Gene-gene interaction between tuberculosis candidate genes in a South African population
    Erika de Wit
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and the DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa
    Mamm Genome 22:100-10. 2011
    ..Methods for studying gene-gene interactions are based on a multilocus and multigene approach, consistent with the nature of complex-trait diseases, and may provide the paradigm for future genetic studies of TB...
  66. ncbi request reprint Drug susceptibility testing using molecular techniques can enhance tuberculosis diagnosis
    Rabia Johnson
    DST NRF Centre of Excellence in Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Department of Biomedical Science, Tygerberg, Stellenbosch University, South Africa
    J Infect Dev Ctries 2:40-5. 2008
    ..The aim of this study was to evaluate the performance and time to diagnosis of a genotypic drug susceptibility testing method...
  67. doi request reprint Genome-wide analysis of the structure of the South African Coloured Population in the Western Cape
    Erika de Wit
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505, South Africa
    Hum Genet 128:145-53. 2010
    ..This is consistent with historical data. While of great historical and genealogical interest, this information is also essential for future admixture mapping of disease genes in this population...
  68. doi request reprint Association of toll-like receptors with susceptibility to tuberculosis suggests sex-specific effects of TLR8 polymorphisms
    Muneeb Salie
    MRC Centre for Tuberculosis Research and the DST NRF Centre of Excellence for Biomedical TB Research, Division of Molecular Biology and Human Genetics, Stellenbosch University, Tygerberg 7505, South Africa
    Infect Genet Evol 34:221-9. 2015
    ..Toll-like receptors (TLRs) are involved in the recognition of conserved microbial structures, leading to activation of an inflammatory response and formation of an adaptive immune response...
  69. pmc Tuberculosis reinfection rate as a proportion of total infection rate correlates with the logarithm of the incidence rate: a mathematical model
    Pieter W Uys
    DST NRF Centre of Excellence for Excellence in Epidemiological Modelling and Analysis SACEMA, Stellenbosch University, Stellenbosch, Western Cape 7602, Republic of South Africa
    J R Soc Interface 6:11-5. 2009
    ..Awareness of this may assist in attempts to control the epidemic...
  70. pmc A panel of ancestry informative markers for the complex five-way admixed South African coloured population
    Michelle Daya
    Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and the DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
    PLoS ONE 8:e82224. 2013
    ..We show that a panel of 96 AIMs can be used to assess ancestry proportions and to adjust for the confounding effect of the complex five-way admixture that occurred in the South African Coloured population...
  71. pmc Ergothioneine is a secreted antioxidant in Mycobacterium smegmatis
    Carine Sao Emani
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Stellenbosch, South Africa
    Antimicrob Agents Chemother 57:3202-7. 2013
    ..smegmatis against oxidative stress and that ERG is able to partly compensate for the loss of MSH...
  72. ncbi request reprint Polymorphisms and mutations found in the regions flanking exons 5 to 8 of the TP53 gene in a population at high risk for esophageal cancer in South Africa
    Marelette Vos
    University of Stellenbosch, MRC Center for Molecular and Cellular Biology, Department of Medical Biochemistry, P O Box 19063, 7505, Tygerberg, South Africa
    Cancer Genet Cytogenet 140:23-30. 2003
    ..The point mutations include the polymorphism in codon 72 (Arg-->Pro), which has recently been associated with an increased risk of developing human papilloma virus-associated cancers...
  73. pmc Beijing and Haarlem genotypes are overrepresented among children with drug-resistant tuberculosis in the Western Cape Province of South Africa
    Ben J Marais
    Ukwanda Centre for Rural Health, Faculty of Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg 7505, South Africa
    J Clin Microbiol 44:3539-43. 2006
    ..The overrepresentation of Beijing and Haarlem genotype families in children with drug-resistant TB demonstrates their contribution to transmitted drug resistance and their potential importance in the emergent drug-resistant TB epidemic...
  74. doi request reprint Multiple, independent, identical IS6110 insertions in Mycobacterium tuberculosis PPE genes
    Christopher R E McEvoy
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    Tuberculosis (Edinb) 89:439-42. 2009
    ..Here we provide evidence for multiple independent IS6110 insertions into identical, or near-identical, positions within the highly homologous 5' region of 3 PPE genes...
  75. doi request reprint Detection of Mycobacterium tuberculosis infection in chacma baboons (Papio ursinus) using the QuantiFERON-TB gold (in-tube) assay
    Sven D C Parsons
    DST NRF Centre of Excellence for Biomedical TB Research MRC Centre for Molecular and Cellular Biology Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, Cape Town, South Africa
    J Med Primatol 38:411-7. 2009
    ..Early diagnosis of simian tuberculosis (TB) is vital to prevent transmission of this disease. We evaluated the ability of the QuantiFERON-TB Gold (In-Tube Method) assay (QFG-IT) to detect TB in chacma baboons (Papio ursinus)...
  76. doi request reprint Mixed infections of Corynebacterium pseudotuberculosis and non-tuberculous mycobacteria in South African antelopes presenting with tuberculosis-like lesions
    Borna Muller
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Stellenbosch University, Francie van Zyl Avenue, Tygerberg 7505, Cape Town, South Africa
    Vet Microbiol 147:340-5. 2011
    ..In cases where potentially pathogenic NTMs are isolated from mycobacterial cultures of tuberculosis-like lesions, the non-use of additional non-selective culture techniques could lead to misinterpretations of the diagnostic test results...
  77. pmc Host markers in QuantiFERON supernatants differentiate active TB from latent TB infection: preliminary report
    Novel N Chegou
    Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, University of Stellenbosch, Cape Town, Western Cape Province, Tygerberg 7505, South Africa
    BMC Pulm Med 9:21. 2009
    ..These assays however, do not discriminate between latent TB infection (LTBI) and active TB disease...
  78. ncbi request reprint Reduction of the rate of false-positive cultures of Mycobacterium tuberculosis in a laboratory with a high culture positivity rate
    Nora M Carroll
    MRC Centre for Cellular and Molecular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, University of Stellenbosch, Tygerberg, South Africa
    Clin Chem Lab Med 40:888-92. 2002
    ..1%. These modifications did not increase the workload or the cost of processing samples and can thus be used successfully by any laboratory, and particularly by those in resource-poor settings...
  79. ncbi request reprint Molecular detection of early appearance of drug resistance during Mycobacterium tuberculosis infection
    Thomas C Victor
    MRC Center for Molecular and Cellular Biology, Department of Medical Biochemistry, University of Stellenbosch, Tygerberg, South Africa
    Clin Chem Lab Med 40:876-81. 2002
    ....
  80. ncbi request reprint On the nature of genetic changes required for the development of esophageal cancer
    Pieter Uys
    MRC Centre for Molecular and Cellular Biology and Department of Medical Biochemistry, University of Stellenbosch, Tygerberg, South Africa
    Mol Carcinog 36:82-9. 2003
    ..The gradual accumulation of aberrant gene function also can explain the progression of pathologic states seen in the esophagus, from early dysplasia through mild to severe dysplasia and, finally, to cancer, as illustrated in our model...
  81. pmc Medroxyprogesterone acetate alters Mycobacterium bovis BCG-induced cytokine production in peripheral blood mononuclear cells of contraceptive users
    Léanie Kleynhans
    Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, DST NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    PLoS ONE 6:e24639. 2011
    ....
  82. ncbi request reprint Association between tuberculosis and a polymorphic NFkappaB binding site in the interferon gamma gene
    Manda Rossouw
    MRC Centre for Molecular and Cellular Biology and Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, 7505, Tygerberg, South Africa
    Lancet 361:1871-2. 2003
    ..This preferential binding suggests that genetically determined variability in interferon gamma and expression might be important for the development of tuberculosis...
  83. pmc Multiple Mycobacterium tuberculosis strains in early cultures from patients in a high-incidence community setting
    Madalene Richardson
    MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa
    J Clin Microbiol 40:2750-4. 2002
    ..This study further investigated these cases with disease caused by multiple M. tuberculosis strains in the context of the molecular epidemiology of the study setting...
  84. doi request reprint Measuring minimum inhibitory concentrations in mycobacteria
    Frederick A Sirgel
    Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, Stellenbosch University, Tygerberg, South Africa
    Methods Mol Biol 465:173-86. 2009
    ..DST concepts and alternative procedures that have been adopted for DST are also briefly discussed...
  85. ncbi request reprint The spectrum of mutations in TP53 in laryngeal cancer patients from a high-incidence population shows similarities to many of the known mutational hotspots
    Desire Barnard
    Department of Medical Biochemistry, MRC Centre for Molecular and Cellular Biology, University of Stellenbosch, PO Box 19063, Tygerberg 7505, South Africa
    Cancer Genet Cytogenet 145:126-32. 2003
    ..The data confirm that the region between codons 175 and 273 of TP53 is a mutational hotspot region for cancers in general; six novel mutations were found within this same region...
  86. ncbi request reprint NAT2 slow acetylator function as a risk indicator for age-related cataract formation
    David Meyer
    Department of Ophthalmology, Faculty of Health Sciences, University of Stellenbosch, PO Box 19063, Tygerberg 7505, South Africa
    Pharmacogenetics 13:285-9. 2003
    ..To show that the slow arylamine N-acetyltransferase type 2 (NAT2) catalysed acetylator function is associated with the development of age-related cataracts...
  87. doi request reprint Detecting drug-resistant tuberculosis: the importance of rapid testing
    Kim G P Hoek
    Division of Medical Microbiology, Department of Pathology, University of Stellenbosch, Tygerberg, South Africa
    Mol Diagn Ther 15:189-94. 2011
    ....
  88. ncbi request reprint Prediction of drug resistance in M. tuberculosis: molecular mechanisms, tools, and applications
    Thomas C Victor
    MRC Centre for Molecular and Cellular Biology and Department of Medical Biochemistry, University of Stellenbosch, Tygerberg, South Africa
    IUBMB Life 53:231-7. 2002
    ..This review focuses on molecular mechanisms, methods and applications as a possible new diagnostic tool for the early molecular detection of drug resistance in M. tuberculosis...
  89. ncbi request reprint Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon
    William R Bourn
    DST NRF Centre of Excellence for Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg 7505, South Africa
    Tuberculosis (Edinb) 87:481-8. 2007
    ..We argue that the mutation exerts its effect through altered RNA folding, thereby affecting the translationally coupled RepA-RepB expression...
  90. ncbi request reprint Changes in the kinetics of intracellular IFN-gamma production in TB patients during treatment
    Hanne Veenstra
    Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology and the DST NRF Centre of Excellence for Biomedical TB Research, University of Stellenbosch, PO Box 19063, 7505 Tygerberg, South Africa
    Clin Immunol 124:336-44. 2007
    ..At diagnosis intracellular IFN-gamma or lymphocyte proliferation was not an indicator for treatment response...
  91. ncbi request reprint Total antioxidant levels are low during active TB and rise with anti-tuberculosis therapy
    I Wiid
    MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, University of Stellenbosch Faculty of Health Sciences, PO Box 19063, Tygerberg 7505, South Africa
    IUBMB Life 56:101-6. 2004
    ..We suggest that total antioxidant status of TB patients should be considered for more effective disease control and that diets low in antioxidants may render individuals susceptible to tuberculosis...
  92. ncbi request reprint Length variation of DC-SIGN and L-SIGN neck-region has no impact on tuberculosis susceptibility
    Luis B Barreiro
    CNRS FRE2849, Unit of Human Evolutionary Genetics, Institut Pasteur, Paris, France
    Hum Immunol 68:106-12. 2007
    ....
  93. ncbi request reprint Strain families of Mycobacterium tuberculosis
    Paul D van Helden
    Trends Microbiol 10:167-8; author reply 168. 2002
  94. ncbi request reprint HIV-1 and tuberculosis infection
    Robin M Warren
    Lancet 359:1618-9; author reply 1619-20. 2002
  95. doi request reprint Predicting reinfection in tuberculosis
    Paul D van Helden
    J Infect Dis 197:172-3; author reply 173-4. 2008
  96. ncbi request reprint Gene-expression patterns in whole blood identify subjects at risk for recurrent tuberculosis
    Rohit Mistry
    GlaxoSmithKline, Stevenage, UK
    J Infect Dis 195:357-65. 2007
    ..However, approximately 5% subsequently have a repeat disease episode, usually within 2 years of successful combination therapy. Presently, there is no way of predicting which patients will experience a relapse...
  97. pmc Application of sensitive and specific molecular methods to uncover global dissemination of the major RDRio Sublineage of the Latin American-Mediterranean Mycobacterium tuberculosis spoligotype family
    Andrea L Gibson
    Cornell University, Joan and Sanford I Weill Medical College, Department of Medicine, Division of International Medicine and Infectious Diseases, Rm A 421, 525 East 68th St, New York, NY 10065, USA
    J Clin Microbiol 46:1259-67. 2008
    ..Our provision of simple and robust molecular methods will assist the evaluation of the LAM family and the RD(Rio) sublineage...
  98. ncbi request reprint The "source" of drug-resistant TB outbreaks
    Paul D van Helden
    Science 314:419-20. 2006
  99. pmc Reinfection and mixed infection cause changing Mycobacterium tuberculosis drug-resistance patterns
    Annelies Van Rie
    Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Am J Respir Crit Care Med 172:636-42. 2005
    ..Multiple infections with different strains of Mycobacterium tuberculosis may occur in settings where the infection pressure is high. The relevance of mixed infections for the patient, clinician, and control program remains unclear...
  100. ncbi request reprint Melatonin prevents the free radical and MADD metabolic profiles induced by antituberculosis drugs in an animal model
    Du Toit Loots
    Department of Nutrition, School for Physiology, Nutrition and Consumer Science, North West University, Potchefstroom, South Africa
    J Pineal Res 38:100-6. 2005
    ..We also conclude that free radicals leading to clinical symptoms associated with an MADD metabolic profile induced by anti-TB treatment could be alleviated by melatonin intervention...
  101. pmc Stability of variable-number tandem repeats of mycobacterial interspersed repetitive units from 12 loci in serial isolates of Mycobacterium tuberculosis
    Evgueni Savine
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, F 59019 Lille cedex, France
    J Clin Microbiol 40:4561-6. 2002
    ..Moreover, they support MIRU VNTR genotyping as a powerful first-line method followed by subtyping by IS6110 RFLP to define ongoing transmission clusters...