Jianxing Song

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. ncbi The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain
    Jiahai Shi
    Department of Biochemistry, The Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    FEBS J 273:1035-45. 2006
  2. pmc Protein dynamics at Eph receptor-ligand interfaces as revealed by crystallography, NMR and MD simulations
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore
    BMC Biophys 5:2. 2012
  3. pmc A small molecule agonist of EphA2 receptor tyrosine kinase inhibits tumor cell migration in vitro and prostate cancer metastasis in vivo
    Aaron Petty
    Rammelkamp Center for Research and Department of Medicine, MetroHealth Campus, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA
    PLoS ONE 7:e42120. 2012
  4. doi Insight into "insoluble proteins" with pure water
    Jianxing Song
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    FEBS Lett 583:953-9. 2009
  5. ncbi Tyrosine phosphorylation of the well packed ephrinB cytoplasmic beta-hairpin for reverse signaling. Structural consequences and binding properties
    Jianxing Song
    Departments of Biochemistry and Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore
    J Biol Chem 278:24714-20. 2003
  6. pmc Mechanism for controlling the dimer-monomer switch and coupling dimerization to catalysis of the severe acute respiratory syndrome coronavirus 3C-like protease
    Jiahai Shi
    Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Republic of Singapore
    J Virol 82:4620-9. 2008
  7. pmc Structural characterization of the EphA4-Ephrin-B2 complex reveals new features enabling Eph-ephrin binding promiscuity
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore
    J Biol Chem 285:644-54. 2010
  8. pmc Resurrecting abandoned proteins with pure water: CD and NMR studies of protein fragments solubilized in salt-free water
    Minfen Li
    Department of Biological Sciences, Faculty of Science, Yong Loo Lin School of Mediciine, National Univeristy of Singapore, Singapore
    Biophys J 91:4201-9. 2006
  9. ncbi Structural insight into the binding diversity between the Tyr-phosphorylated human ephrinBs and Nck2 SH2 domain
    Xiaoyuan Ran
    Department of Biochemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    J Biol Chem 280:19205-12. 2005
  10. pmc Insights into protein aggregation by NMR characterization of insoluble SH3 mutants solubilized in salt-free water
    Jingxian Liu
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    PLoS ONE 4:e7805. 2009

Collaborators

  • Wei Wang
  • Zheng Wei
  • Yee Joo Tan
  • Yuguang Mu
  • Roberta Noberini
  • Elena Pasquale
  • Jianping Wu
  • Ed Manser
  • Alexander D MacKerell
  • Sohail Ahmed
  • Shu Wang
  • Seong Loong Lo
  • Andrew C A Wan
  • Bor Luen Tang
  • Y Adam Yuan
  • Yang Liu
  • Masayo Kotaka
  • Zhenbao Yu
  • Haina Qin
  • Liangzhong Lim
  • Jiahai Shi
  • Jingxian Liu
  • Garvita Gupta
  • Minfen Li
  • Yuanyuan Wei
  • Shixiong Lua
  • Xiaoyuan Ran
  • Shaveta Goyal
  • Xuelu Huan
  • Bhaskar Barnwal
  • Liang Zhong Lim
  • Steven Setiawan Theng
  • Aaron Petty
  • Sujit Dutta
  • Jingye Yang
  • Linlin Miao
  • Wanlong Zhu
  • Vincent T K Chow
  • Goyal Shaveta
  • J Sivaraman
  • Jing Song Fan
  • Lu Wang
  • Yimei Lu
  • Vincent Tak Kwong Chow
  • Chee Keng Mok
  • Mandakhalikar Kedar Diwakar
  • Qi Zeng
  • Xiaowen Lee
  • Cheryl Chan
  • Yun Gao
  • Way Champ Mah
  • Caroline G Lee
  • Samuel S Chong
  • Jingli Zhuo
  • Sayantan Mitra
  • Eugene Choo Shi Guang
  • Gregory P Tochtrop
  • Bingcheng Wang
  • Yook Wah Choi
  • Eugene Myshkin
  • Jackie Y Ying
  • Jer Tsong Hsieh
  • Lili Liu
  • Phillip Page
  • Jun Min Xue
  • Chayan Acharya
  • Hui Miao
  • Burtram C Fielding
  • Eckhard Ficker
  • Daniel J Lindner
  • Ghayathri Balasundaram
  • Hong Guo
  • Megha Haridas Upadya
  • Patrice Koehl
  • Lushan Wang
  • Nanyu Han
  • Justina Shihui Tong
  • Fading Chen
  • Satoru Machida
  • Helen X Pu
  • Xiangyang Liu
  • Yali Li
  • Ning Du
  • Xuanhao Liao
  • Zhi Cheng Xiao
  • Miaoqing Fang
  • June Mui Goh
  • Felicia Y H Teng

Detail Information

Publications53

  1. ncbi The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain
    Jiahai Shi
    Department of Biochemistry, The Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    FEBS J 273:1035-45. 2006
    ..We therefore speculate that this channel might play a role in relaying regulatory effects from the extra domain to the catalytic machinery...
  2. pmc Protein dynamics at Eph receptor-ligand interfaces as revealed by crystallography, NMR and MD simulations
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore
    BMC Biophys 5:2. 2012
    ..abstract:..
  3. pmc A small molecule agonist of EphA2 receptor tyrosine kinase inhibits tumor cell migration in vitro and prostate cancer metastasis in vivo
    Aaron Petty
    Rammelkamp Center for Research and Department of Medicine, MetroHealth Campus, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA
    PLoS ONE 7:e42120. 2012
    ..To our knowledge, doxazosin is the first small molecule agonist of a receptor tyrosine kinase that is capable of inhibiting malignant behaviors in vitro and in vivo...
  4. doi Insight into "insoluble proteins" with pure water
    Jianxing Song
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    FEBS Lett 583:953-9. 2009
    ..In this review, I discuss our results in a wide context and subsequently propose a model to rationalize the discovery. The potential applications are also explored in studying protein folding, design and membrane proteins...
  5. ncbi Tyrosine phosphorylation of the well packed ephrinB cytoplasmic beta-hairpin for reverse signaling. Structural consequences and binding properties
    Jianxing Song
    Departments of Biochemistry and Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore
    J Biol Chem 278:24714-20. 2003
    ....
  6. pmc Mechanism for controlling the dimer-monomer switch and coupling dimerization to catalysis of the severe acute respiratory syndrome coronavirus 3C-like protease
    Jiahai Shi
    Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Republic of Singapore
    J Virol 82:4620-9. 2008
    ..Remarkably, dimerization appears to be coupled to catalysis in 3CLpro through the use of overlapped residues for two networks, one for dimerization and another for the catalysis...
  7. pmc Structural characterization of the EphA4-Ephrin-B2 complex reveals new features enabling Eph-ephrin binding promiscuity
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore
    J Biol Chem 285:644-54. 2010
    ..Our findings shed light on the distinctive features that enable the remarkable ligand binding promiscuity of EphA4 and suggest that diverse strategies are needed to effectively disrupt different Eph-ephrin complexes...
  8. pmc Resurrecting abandoned proteins with pure water: CD and NMR studies of protein fragments solubilized in salt-free water
    Minfen Li
    Department of Biological Sciences, Faculty of Science, Yong Loo Lin School of Mediciine, National Univeristy of Singapore, Singapore
    Biophys J 91:4201-9. 2006
    ..Our discovery not only gives a novel insight into the properties of insoluble proteins, but also sheds the first light that we know of on previously unknown regimes associated with proteins...
  9. ncbi Structural insight into the binding diversity between the Tyr-phosphorylated human ephrinBs and Nck2 SH2 domain
    Xiaoyuan Ran
    Department of Biochemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    J Biol Chem 280:19205-12. 2005
    ..In contrast, binding with [Tyr(P)330]ephrinB2(324-333) might have most residues over both halves engaged but induced less profound conformational dynamics on the mus-ms time scale...
  10. pmc Insights into protein aggregation by NMR characterization of insoluble SH3 mutants solubilized in salt-free water
    Jingxian Liu
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    PLoS ONE 4:e7805. 2009
    ..Furthermore, a general mechanism is also proposed for in vivo protein aggregation triggered by the genetic mutation and posttranslational modification...
  11. doi NMR structure and dynamics of human ephrin-B2 ectodomain: the functionally critical C-D and G-H loops are highly dynamic in solution
    Xiaoyuan Ran
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Proteins 72:1019-29. 2008
    ..The present results may bear critical implications in understanding the molecular details as well as designing antagonists of therapeutic interest for Eph-ephrin interactions...
  12. doi Identification and structural mechanism for a novel interaction between a ubiquitin ligase WWP1 and Nogo-A, a key inhibitor for central nervous system regeneration
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Biochemistry 47:13647-58. 2008
    ..0. This study provides rationales as well as a template Nogo-A(650-666) for further design of molecules to intervene in the WWP1-Nogo-A interaction which may regulate the Nogo-A protein level by controlling its ubiquitination...
  13. pmc Structural, stability, dynamic and binding properties of the ALS-causing T46I mutant of the hVAPB MSP domain as revealed by NMR and MD simulations
    Shixiong Lua
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e27072. 2011
    ....
  14. ncbi Structural insight into the binding diversity between the human Nck2 SH3 domains and proline-rich proteins
    Jingxian Liu
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Biochemistry 45:7171-84. 2006
    ..Interestingly, of the three SH3-binding motifs carried by Wrch1, only the middle one was capable of binding SH3-2. Our results provide valuable clues for further functional investigations into the Nck2-mediated signaling networks...
  15. doi Rational design, solution conformation and identification of functional residues of the soluble and structured Nogo-54, which mimics Nogo-66 in inhibiting the CNS neurite outgrowth
    Minfen Li
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Biochem Biophys Res Commun 373:498-503. 2008
    ..Furthermore, our study also provides a soluble and structured mimic as well as a possible antagonist for Nogo-66 which may hold promising potential for various medical applications...
  16. pmc Crystal structure and NMR binding reveal that two small molecule antagonists target the high affinity ephrin-binding channel of the EphA4 receptor
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 11926
    J Biol Chem 283:29473-84. 2008
    ..Furthermore, the results demonstrate that the high affinity ephrin binding channel of the Eph receptors is amenable to targeting with small molecule antagonists and suggest avenues for further optimization...
  17. pmc Identification of a novel nonstructural protein, VP9, from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites
    Yang Liu
    Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543
    J Virol 80:10419-27. 2006
    ..Based on our present investigations, we hypothesize that VP9 might be involved in the transcriptional regulation of WSSV, a function similar to that of the E2 protein during papillomavirus infection of the host cells...
  18. pmc Unique structure and dynamics of the EphA5 ligand binding domain mediate its binding specificity as revealed by X-ray crystallography, NMR and MD simulations
    Xuelu Huan
    NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Republic of Singapore
    PLoS ONE 8:e74040. 2013
    ..Our findings also suggest that a promising strategy to design agonists/antagonists with high affinity and selectivity might be to target specific dynamic states of the Eph receptor LBDs. ..
  19. pmc Nogo goes in the pure water: solution structure of Nogo-60 and design of the structured and buffer-soluble Nogo-54 for enhancing CNS regeneration
    Minfen Li
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Protein Sci 15:1835-41. 2006
    ..Furthermore, our discovery may imply a general solution to solubilizing a category of buffer-insoluble proteins for urgent structural investigations...
  20. doi Elimination of the native structure and solubility of the hVAPB MSP domain by the Pro56Ser mutation that causes amyotrophic lateral sclerosis
    Jiahai Shi
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Biochemistry 49:3887-97. 2010
    ..Our study thus implies that the Pro56Ser mutation might lead to ALS by eliminating the native MSP structure, which consequently leads to aggregation and loss of functions under physiological conditions...
  21. pmc VAPC, an human endogenous inhibitor for hepatitis C virus (HCV) infection, is intrinsically unstructured but forms a "fuzzy complex" with HCV NS5B
    Shaveta Goyal
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore
    PLoS ONE 7:e40341. 2012
    ....
  22. doi NMR binding and crystal structure reveal that intrinsically-unstructured regulatory domain auto-inhibits PAK4 by a mechanism different from that of PAK1
    Wei Wang
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biochem Biophys Res Commun 438:169-74. 2013
    ..Our study reveals that PAK4 is auto-inhibited by a novel mechanism which is completely different from that for PAK1, thus bearing critical implications for design of inhibitors specific for group II PAKs...
  23. pmc NMR evidence for forming highly populated helical conformations in the partially folded hNck2 SH3 domain
    Jingxian Liu
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119260, Singapore
    Biophys J 95:4803-12. 2008
    ..0. This observation implies that the region might contribute key interactions to disrupt the helical state, and to facilitate a further transformation into the native SH3 fold in the second transition...
  24. ncbi Molecular mechanism underlying the thermal stability and pH-induced unfolding of CHABII
    Zheng Wei
    Department of Biological Sciences, National University of Singapore, 10 Kent Ridge, Crescent, Singapore 119260
    J Mol Biol 348:205-18. 2005
    ..The enhancement led to an increase of the thermal stability of [Phe21]-CHABII by approximately 17 deg. C...
  25. doi Selective and specific ion binding on proteins at physiologically-relevant concentrations
    Linlin Miao
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    FEBS Lett 585:3126-32. 2011
    ..We reveal however that their 8 anions bind to the ephrin-B2 protein with high affinity and specificity at biologically-relevant concentrations. Interestingly, the binding is found to be both salt- and residue-specific...
  26. doi Identification, recombinant production and structural characterization of four silk proteins from the Asiatic honeybee Apis cerana
    Jiahai Shi
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biomaterials 29:2820-8. 2008
    ....
  27. pmc Intrinsically unstructured domain 3 of hepatitis C Virus NS5A forms a "fuzzy complex" with VAPB-MSP domain which carries ALS-causing mutations
    Garvita Gupta
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Republic of Singapore
    PLoS ONE 7:e39261. 2012
    ....
  28. ncbi Dissection study on the severe acute respiratory syndrome 3C-like protease reveals the critical role of the extra domain in dimerization of the enzyme: defining the extra domain as a new target for design of highly specific protease inhibitors
    Jiahai Shi
    Department of Biochemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    J Biol Chem 279:24765-73. 2004
    ....
  29. pmc Dynamically-driven inactivation of the catalytic machinery of the SARS 3C-like protease by the N214A mutation on the extra domain
    Jiahai Shi
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    PLoS Comput Biol 7:e1001084. 2011
    ..Consequently, mediating the dynamics may offer a potential avenue to inhibit the SARS-CoV 3CLpro...
  30. doi ALS-causing P56S mutation and splicing variation on the hVAPB MSP domain transform its β-sandwich fold into lipid-interacting helical conformations
    Haina Qin
    Department of Biological Sciences, Faculty of Science, Yong Loo Lin School of Medicine and National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biochem Biophys Res Commun 431:398-403. 2013
    ..Our discovery provides a mechanism for ALS-causing VAPB mutants/variants to gain novel functions such as to mediate ER structure before significant accumulation of aggregates occurs...
  31. doi NMR studies reveal a novel mode for hFADD to bind with the unstructured hRTN3 which initiates the ER-stress activated apoptosis
    Jingxian Liu
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biochem Biophys Res Commun 383:433-9. 2009
    ..This study also implies a possible linkage between Alzheimer's disease and ER-stress activated apoptosis...
  32. ncbi The N- and C-termini of the human Nogo molecules are intrinsically unstructured: bioinformatics, CD, NMR characterization, and functional implications
    Minfen Li
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Proteins 68:100-8. 2007
    ..We propose that this mechanism may represent a general strategy to place the formation/maintenance of membrane-related structures under the direct regulation of the cellular signaling...
  33. ncbi Structural characterization of the human Nogo-A functional domains. Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration
    Minfen Li
    Department of Biological Sciences, National University of Singapore, Singapore
    Eur J Biochem 271:3512-22. 2004
    ..Our results provide the first structural insights into Nogo-A functional domains and may have implications in further designs of peptide mimetics that would enhance CNS neuronal regeneration...
  34. pmc Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction
    Haina Qin
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    RNA 16:474-81. 2010
    ..These data suggest a potential functional role of the Arabidopsis DUF283 domain in target selection in small RNA processing...
  35. ncbi Nogo-B receptor possesses an intrinsically unstructured ectodomain and a partially folded cytoplasmic domain
    Minfen Li
    Department of Biological Sciences, Faculty of Science, Yong Loo Lin School of Medicine and National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore
    Biochem Biophys Res Commun 360:128-34. 2007
    ....
  36. doi Inter-domain interactions of TDP-43 as decoded by NMR
    Yuanyuan Wei
    NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore
    Biochem Biophys Res Commun 473:614-9. 2016
    ..Our study thus offers a mechanism by which cellular processes might control TDP-43 physiology and proteinopathy by mediating its inter-domain interactions. ..
  37. pmc ALS-Causing Mutations Significantly Perturb the Self-Assembly and Interaction with Nucleic Acid of the Intrinsically Disordered Prion-Like Domain of TDP-43
    Liangzhong Lim
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    PLoS Biol 14:e1002338. 2016
    ....
  38. doi A monoclonal antibody binds to threonine 49 in the non-structural 1 protein of influenza A virus and interferes with its ability to modulate viral replication
    Bhaskar Barnwal
    Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System NUHS, National University of Singapore, Singapore
    Antiviral Res 116:55-61. 2015
    ..In future, 2H6 antibody in combination with other neutralizing antibodies can be used to increase the potency of viral inhibition. ..
  39. doi Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR)
    Shaveta Goyal
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260, Singapore
    Methods Mol Biol 1258:371-85. 2015
    ..In this chapter, we provide a detailed procedure to express and purify these proteins, followed by CD and NMR spectroscopy characterization of their conformation and interaction with dodecylphosphocholine (DPC). ..
  40. doi Structural characterization reveals that viperin is a radical S-adenosyl-L-methionine (SAM) enzyme
    Goyal Shaveta
    Department of Biochemistry, Yong Loo Lin School of Medicine, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biochem Biophys Res Commun 391:1390-5. 2010
    ..Our results thus imply that the radical SAM enzyme activity may play a key role in the broad antiviral actions of viperin...
  41. pmc Disruption of FAT10-MAD2 binding inhibits tumor progression
    Steven Setiawan Theng
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119077, Singapore National University of Singapore Graduate School of Integrative Sciences and Engineering, National University of Singapore, Singapore 119077, Singapore
    Proc Natl Acad Sci U S A 111:E5282-91. 2014
    ..Our results strongly suggest that the interaction of FAT10 with MAD2 is a key mechanism underlying the promalignant property of FAT10 and offer prospects for the development of anticancer strategies. ..
  42. pmc Dynamically-driven enhancement of the catalytic machinery of the SARS 3C-like protease by the S284-T285-I286/A mutations on the extra domain
    Liangzhong Lim
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Republic of Singapore
    PLoS ONE 9:e101941. 2014
    ..Our results thus imply a promising avenue to design specific inhibitors for 3CL proteases by disrupting their dynamic correlation network. ..
  43. ncbi A novel nucleolar transcriptional activator ApLLP for long-term memory formation is intrinsically unstructured but functionally active
    Jingxian Liu
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Biochem Biophys Res Commun 366:585-91. 2008
    ..Taken together, our results define ApLLP as an intrinsically unstructured protein and may bear important implications in understanding the molecular mechanism underlying ApLLP functions...
  44. pmc C-Terminal Auto-Regulatory Motif of Hepatitis C Virus NS5B Interacts with Human VAPB-MSP to Form a Dynamic Replication Complex
    Garvita Gupta
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    PLoS ONE 11:e0147278. 2016
    ....
  45. pmc NMR and MD Studies Reveal That the Isolated Dengue NS3 Protease Is an Intrinsically Disordered Chymotrypsin Fold Which Absolutely Requests NS2B for Correct Folding and Functional Dynamics
    Garvita Gupta
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore, Singapore
    PLoS ONE 10:e0134823. 2015
    ..Taken together, our study implies potential strategies to perturb the NS2B-NS3pro interface for design of inhibitors for treating dengue infection. ..
  46. doi Dynamic principle for designing antagonistic/agonistic molecules for EphA4 receptor, the only known ALS modifier
    Haina Qin
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    ACS Chem Biol 10:372-8. 2015
    ..For example, to increase the survival of ALS patients by inhibiting EphA4, the drugs must enhance, or at least not suppress, the EphA4 dynamics. ..
  47. doi Microfibers fabricated by non-covalent assembly of peptide and DNA for viral vector encapsulation and cancer therapy
    Jingye Yang
    Institute of Bioengineering and Nanotechnology, Singapore, Singapore
    Adv Mater 24:3280-4. 2012
    ..Since the fiber formation process takes place under ambient conditions and is aqueous-based without the use of denaturing organic solvents, the bioactivity of viruses is well preserved...
  48. doi The variable N-terminal region of DDX5 contains structural elements and auto-inhibits its interaction with NS5B of hepatitis C virus
    Sujit Dutta
    Institute of Molecular and Cell Biology, A STAR Agency for Science, Technology and Research, Singapore 138673
    Biochem J 446:37-46. 2012
    ..Furthermore, NMR investigations reveal that there is a direct interaction between DDX5 and NS5B in vitro...
  49. doi SALS-linked WT-SOD1 adopts a highly similar helical conformation as FALS-causing L126Z-SOD1 in a membrane environment
    Liangzhong Lim
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Republic of Singapore
    Biochim Biophys Acta 1858:2223-30. 2016
    ..Moreover, any genetic/environmental factors which can delay or impair its maturation might act to transform SOD1 into cytotoxic forms with the acquired capacity to abnormally interact with membranes. ..
  50. doi Mechanism for transforming cytosolic SOD1 into integral membrane proteins of organelles by ALS-causing mutations
    Liangzhong Lim
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Republic of Singapore
    Biochim Biophys Acta 1848:1-7. 2015
    ..That zinc-deficiency is sufficient to produce a similar transformation in wild-type SOD1 implies that the wild-type and FALS-linked SOD1 mutants may trigger ALS by a common mechanism. ..
  51. pmc TDP-43 N terminus encodes a novel ubiquitin-like fold and its unfolded form in equilibrium that can be shifted by binding to ssDNA
    Haina Qin
    Department of Biological Sciences, Faculty of Science, and
    Proc Natl Acad Sci U S A 111:18619-24. 2014
    ..As a consequence, one therapeutic strategy for TDP-43-causing diseases might be to stabilize its ubiquitin-like fold by ssDNA or designed molecules. ..
  52. ncbi Stability of protein-bound conformations of bioactive peptides: the folded conformation of an epidermal growth factor-like thrombomodulin fragment is similar to that recognized by thrombin
    Jianxing Song
    Montreal Joint Centre for Structural Biology, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada
    Biopolymers 65:373-86. 2002
    ....
  53. ncbi Solution structure and backbone dynamics of the functional cytoplasmic subdomain of human ephrin B2, a cell-surface ligand with bidirectional signaling properties
    Jianxing Song
    Biomolecular NMR Group and Mammalian Cells Genetics Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, Canada H4P 2R2
    Biochemistry 41:10942-9. 2002
    ....