M Prakash Hande

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. ncbi request reprint Telomere and telomerase modulation by the mammalian Rad9/Rad1/Hus1 DNA-damage-checkpoint complex
    Sofia Francia
    Italian Foundation for Cancer Research, Institute of Molecular Oncology Foundation, 20139 Milan, Italy
    Curr Biol 16:1551-8. 2006
  2. ncbi request reprint Targeting DNA-PKcs and telomerase in brain tumour cells
    Resham Lal Gurung
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 2 Medical Drive, Singapore 117597, Singapore
    Mol Cancer 13:232. 2014
  3. pmc Thymoquinone induces telomere shortening, DNA damage and apoptosis in human glioblastoma cells
    Resham Lal Gurung
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    PLoS ONE 5:e12124. 2010
  4. pmc Enhanced genotoxicity of silver nanoparticles in DNA repair deficient Mammalian cells
    Hui Kheng Lim
    Genome Stability Laboratory, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore Singapore
    Front Genet 3:104. 2012
  5. pmc Stable expression of promyelocytic leukaemia (PML) protein in telomerase positive MCF7 cells results in alternative lengthening of telomeres phenotype
    Jacklyn W Y Yong
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore
    Genome Integr 3:5. 2012
  6. pmc Differential regulation of intracellular factors mediating cell cycle, DNA repair and inflammation following exposure to silver nanoparticles in human cells
    Pv Asharani
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, 117543, Singapore
    Genome Integr 3:2. 2012
  7. pmc Anti-proliferative activity of silver nanoparticles
    P V AshaRani
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, 117543 Singapore
    BMC Cell Biol 10:65. 2009
  8. ncbi request reprint A role for Brca1 in chromosome end maintenance
    J Peter McPherson
    Advanced Medical Discovery Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2C1
    Hum Mol Genet 15:831-8. 2006
  9. ncbi request reprint DNA damage and p53-mediated growth arrest in human cells treated with platinum nanoparticles
    P V AshaRani
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, Singapore
    Nanomedicine (Lond) 5:51-64. 2010
  10. pmc Inhibition of poly (ADP-Ribose) polymerase-1 in telomerase deficient mouse embryonic fibroblasts increases arsenite-induced genome instability
    Resham L Gurung
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597
    Genome Integr 1:5. 2010

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Telomere and telomerase modulation by the mammalian Rad9/Rad1/Hus1 DNA-damage-checkpoint complex
    Sofia Francia
    Italian Foundation for Cancer Research, Institute of Molecular Oncology Foundation, 20139 Milan, Italy
    Curr Biol 16:1551-8. 2006
    ..These findings identify an unanticipated function for the 911 checkpoint complex at telomeres in mammals and provide a mechanistic link between the activity of DNA-damage-checkpoint proteins and the telomere-maintenance machinery...
  2. ncbi request reprint Targeting DNA-PKcs and telomerase in brain tumour cells
    Resham Lal Gurung
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 2 Medical Drive, Singapore 117597, Singapore
    Mol Cancer 13:232. 2014
    ..Identifying alternative molecular targets would enable us to develop different therapeutic strategies for better management of these tumours...
  3. pmc Thymoquinone induces telomere shortening, DNA damage and apoptosis in human glioblastoma cells
    Resham Lal Gurung
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    PLoS ONE 5:e12124. 2010
    ..Much emphasis has been placed on discovering new compounds that target tumour cells more efficiently and selectively with minimal toxic effects on normal cells...
  4. pmc Enhanced genotoxicity of silver nanoparticles in DNA repair deficient Mammalian cells
    Hui Kheng Lim
    Genome Stability Laboratory, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore Singapore
    Front Genet 3:104. 2012
    ..These findings support the fact that DNA-PKcs is involved in the repair of Ag-np induced genotoxicity and NHEJ repair pathway and DNA-PKcs particularly is activated to safeguard the genome upon Ag-np exposure...
  5. pmc Stable expression of promyelocytic leukaemia (PML) protein in telomerase positive MCF7 cells results in alternative lengthening of telomeres phenotype
    Jacklyn W Y Yong
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore
    Genome Integr 3:5. 2012
    ..abstract:..
  6. pmc Differential regulation of intracellular factors mediating cell cycle, DNA repair and inflammation following exposure to silver nanoparticles in human cells
    Pv Asharani
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, 117543, Singapore
    Genome Integr 3:2. 2012
    ..abstract:..
  7. pmc Anti-proliferative activity of silver nanoparticles
    P V AshaRani
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, 117543 Singapore
    BMC Cell Biol 10:65. 2009
    ..No safety regulations are in practice for the use of these nanomaterials. The interactions of nanomaterials with cells, uptake mechanisms, distribution, excretion, toxicological endpoints and mechanism of action remain unanswered...
  8. ncbi request reprint A role for Brca1 in chromosome end maintenance
    J Peter McPherson
    Advanced Medical Discovery Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2C1
    Hum Mol Genet 15:831-8. 2006
    ..The telomere dysfunction phenotype in Brca1-deficient cells suggests that loss of telomere integrity might contribute to chromosome end dysfunction and permit the formation of potentially oncogenic translocations...
  9. ncbi request reprint DNA damage and p53-mediated growth arrest in human cells treated with platinum nanoparticles
    P V AshaRani
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, Singapore
    Nanomedicine (Lond) 5:51-64. 2010
    ..This study investigates the uptake and bioactivity (e.g., cytotoxicity, genotoxicity and protein expression) of platinum nanoparticles (Pt-NPs, approximately 5-8 nm in size) in human cells...
  10. pmc Inhibition of poly (ADP-Ribose) polymerase-1 in telomerase deficient mouse embryonic fibroblasts increases arsenite-induced genome instability
    Resham L Gurung
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597
    Genome Integr 1:5. 2010
    ..In this study, the effects of combined inhibition of PARP-1 and telomerase in mouse embryonic fibroblasts (MEFs) following sodium arsenite exposure (a carcinogen and potent DNA damaging agent), were evaluated...
  11. ncbi request reprint DNA repair factors and telomere-chromosome integrity in mammalian cells
    M P Hande
    Department of Physiology, Faculty of Medicine, National University of Singapore, and Oncology Research Institute, Singapore, Singapore
    Cytogenet Genome Res 104:116-22. 2004
    ..Because the maintenance of telomere function is crucial for genomic stability, our results will provide new insights into the mechanisms of chromosome instability and tumour formation...
  12. pmc Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells
    Kalpana Gopalakrishnan
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore
    Genome Integr 1:16. 2010
    ..abstract:..
  13. ncbi request reprint The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo
    Y Liu
    Ontario Cancer Institute Amgen Institute, Department of Medical Biophysics, University of Toronto, 620 University Avenue, ON M5G 2C1, Toronto, Canada
    Curr Biol 10:1459-62. 2000
    ..ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT...
  14. pmc Telomerase-associated protein TEP1 is not essential for telomerase activity or telomere length maintenance in vivo
    Y Liu
    Ontario Cancer Institute Amgen Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2C1, Canada
    Mol Cell Biol 20:8178-84. 2000
    ..These results reveal that TEP1 is an RNA binding protein that is not restricted to the telomerase complex and that TEP1 plays a redundant role in the assembly or localization of the telomerase RNP in vivo...
  15. ncbi request reprint Isoform-specific activation of protein kinase c in irradiated human fibroblasts and their bystander cells
    Rajamanickam Baskar
    Center for Radiological Research, Department of Radiation Oncology, College of Physicians and Surgeons, Columbia University, 630 W 168th Street, New York, NY 10032, USA
    Int J Biochem Cell Biol 40:125-34. 2008
    ....
  16. ncbi request reprint Oxidative damage induced genotoxic effects in human fibroblasts from Xeroderma Pigmentosum group A patients
    Grace Kah Mun Low
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, MD9, 2 Medical Drive, Singapore 117597, Singapore
    Int J Biochem Cell Biol 40:2583-95. 2008
    ..Lack of functional Xeroderma Pigmentosum A seems to increase the susceptibility of oxidative stress-induced genotoxicity while retaining cell viability posing as a potential cancer risk factor of Xeroderma Pigmentosum A patients...
  17. ncbi request reprint Functional interplay of p53 and Mus81 in DNA damage responses and cancer
    Ashwin Pamidi
    The Advanced Medical Discovery Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
    Cancer Res 67:8527-35. 2007
    ..Furthermore, our data show that loss of Mus81, in addition to p53, is a key step in sarcoma development...
  18. ncbi request reprint Short dysfunctional telomeres impair the repair of arsenite-induced oxidative damage in mouse cells
    Jennifer P A Newman
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    J Cell Physiol 214:796-809. 2008
    ..Results from our studies imply that short dysfunctional telomeres impair the repair of oxidative damage caused by arsenite. The results will have implications in risk estimation as well as cancer chemotherapy...
  19. ncbi request reprint Genomic imbalances in key ion channel genes and telomere shortening in sudden cardiac death victims
    B Banerjee
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Cytogenet Genome Res 122:350-5. 2008
    ..Identification of biomarkers of SCD is of great importance and thus the present study will facilitate the identification of some of the biomarkers...
  20. ncbi request reprint Inhibition of telomerase activity and human telomerase reverse transcriptase gene expression by histone deacetylase inhibitor in human brain cancer cells
    Aik Kia Khaw
    Genome Stability Laboratory, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Republic of Singapore
    Mutat Res 625:134-44. 2007
    ..This study suggests that telomerase and hTERT might be the primary targets of TSA which may have the potential to be used as a telomerase inhibitor in cancer therapy...
  21. pmc Collaboration of Brca1 and Chk2 in tumorigenesis
    John Peter McPherson
    Advanced Medical Discovery Institute, Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada
    Genes Dev 18:1144-53. 2004
    ..Furthermore, inactivation of Chk2 and Brca1 was cooperative in breast cancer. Our findings identify a critical role for Chk2 as a component of the DNA damage-signaling pathway activated in response to Brca1 deficiency...
  22. ncbi request reprint Loss of Brca2 and p53 synergistically promotes genomic instability and deregulation of T-cell apoptosis
    Alison M Y Cheung
    Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Ontario, Canada M5G 2M9
    Cancer Res 62:6194-204. 2002
    ....
  23. ncbi request reprint Effects of an integrated yoga program in modulating psychological stress and radiation-induced genotoxic stress in breast cancer patients undergoing radiotherapy
    Birendranath Banerjee
    Genome Stability Laboratory, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Integr Cancer Ther 6:242-50. 2007
    ..An integrated approach of yoga intervention modulates the stress and DNA damage levels in breast cancer patients during radiotherapy...
  24. ncbi request reprint c-Jun N-terminal kinase mediates hydrogen peroxide-induced cell death via sustained poly(ADP-ribose) polymerase-1 activation
    S Zhang
    Department of Community, Occupational and Family Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore
    Cell Death Differ 14:1001-10. 2007
    ....
  25. ncbi request reprint Involvement of mammalian Mus81 in genome integrity and tumor suppression
    John Peter McPherson
    Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, Ontario, Canada M5G 2C1
    Science 304:1822-6. 2004
    ..These studies demonstrate a critical role for the proper biallelic expression of the mammalian Mus81 in the maintenance of genomic integrity and tumor suppression...
  26. ncbi request reprint Lack of poly(ADP-ribose) polymerase-1 gene product enhances cellular sensitivity to arsenite
    Anuradha Poonepalli
    Genome Stability Laboratory, Department of Physiology, National University of Singapore
    Cancer Res 65:10977-83. 2005
    ..Our results suggest an important role for PARP-1 gene product in the maintenance of chromosome-genome stability in response to arsenite-induced DNA damage...
  27. ncbi request reprint Annexin-1 protects MCF7 breast cancer cells against heat-induced growth arrest and DNA damage
    Sunitha Nair
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Singapore
    Cancer Lett 294:111-7. 2010
    ..This study demonstrates that ANXA1, which we recently reported as a possible tumor suppressor gene, can protect cells from heat-induced growth arrest and DNA damage...
  28. ncbi request reprint Progressive loss of epidermal growth factor receptor in a subpopulation of breast cancers: implications in target-directed therapeutics
    Lee Yee Choong
    Oncology Research Institute, National University of Singapore, Singapore
    Mol Cancer Ther 6:2828-42. 2007
    ..Although no value of EGFR expression in prognosis was found, our findings are likely to have implications in the design of clinical trials targeting the EGFR family of proteins in breast cancer...
  29. pmc Lats2/Kpm is required for embryonic development, proliferation control and genomic integrity
    John Peter McPherson
    Division of Cellular and Molecular Biology, Department of Medical Biophysics, Ontario Cancer Institute, University of Toronto, Toronto, Ontario, Canada
    EMBO J 23:3677-88. 2004
    ..These findings indicate an essential role of Lats2 in the integrity of processes that govern centrosome duplication, maintenance of mitotic fidelity and genomic stability...
  30. ncbi request reprint Telomere-mediated genomic instability and the clinico-pathological parameters in breast cancer
    Anuradha Poonepalli
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Genes Chromosomes Cancer 47:1098-109. 2008
    ..The data support the notion that telomere dysfunction might be of value as a marker of aggressiveness of the tumors in breast cancer patients...
  31. ncbi request reprint A role for Mus81 in the repair of chromium-induced DNA damage
    Laura Tamblyn
    Department of Pharmacology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Mutat Res 660:57-65. 2009
    ..Our findings support a role for Mus81 in the resolution of replication-associated DNA damage associated with this genotoxic agent, by converting Cr[VI]-DNA lesions into a form more amenable for homologous recombination...
  32. pmc Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells
    Jacinth Abraham
    Advanced Medical Discovery Institute, Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, Ontario M5G 2C1, Canada
    EMBO J 22:6137-47. 2003
    ..Most importantly, Eme1 deficiency led to spontaneous genomic instability. These results reveal that mammalian Eme1 plays a key role in DNA repair and the maintenance of genome integrity...
  33. ncbi request reprint TAp73 is a downstream target of p53 in controlling the cellular defense against stress
    Jianli Wang
    Department of Radiation Oncology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Biol Chem 282:29152-62. 2007
    ..Our data reveal a new pathway for cellular protection against oxidative damage and provide evidence that TAp73 is a stress-response gene and a downstream effector in the p53 pathway...
  34. ncbi request reprint Synergistic role of Ku80 and poly(ADP-ribose) polymerase in suppressing chromosomal aberrations and liver cancer formation
    Wei Min Tong
    International Agency for Research on Cancer IARC, 69008 Lyon, France
    Cancer Res 62:6990-6. 2002
    ....
  35. pmc Deletion of mouse rad9 causes abnormal cellular responses to DNA damage, genomic instability, and embryonic lethality
    Kevin M Hopkins
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Mol Cell Biol 24:7235-48. 2004
    ..These investigations establish Mrad9 as a key mammalian genetic element of pathways that regulate the cellular response to DNA damage, maintenance of genomic integrity, and proper embryonic development...
  36. ncbi request reprint Complex chromosome aberrations persist in individuals many years after occupational exposure to densely ionizing radiation: an mFISH study
    M Prakash Hande
    Center for Radiological Research, Columbia University Medical Center, New York, USA
    Genes Chromosomes Cancer 44:1-9. 2005
    ..The results suggest that stable complex chromosomal translocations represent a long-lived, quantitative, low-background biomarker of densely ionizing radiation for human populations exposed many years ago...
  37. ncbi request reprint Human embryonic stem cells may display higher resistance to genotoxic stress as compared to primary explanted somatic cells
    Kumar Jayaseelan Vinoth
    Stem Cell Program, Faculty of Dentistry, National University of Singapore, 119074 Singapore
    Stem Cells Dev 17:599-607. 2008
    ..These results highlight possible intrinsic differences in response to damaging agents between hES cells and normal somatic cells...
  38. pmc Past exposure to densely ionizing radiation leaves a unique permanent signature in the genome
    M Prakash Hande
    Center for Radiological Research, Columbia University, New York, NY 10032, USA
    Am J Hum Genet 72:1162-70. 2003
    ..Quantification of this large-scale chromosomal damage in human populations exposed many years earlier will lead to new insights into the mechanisms and risks of cytogenetic damage...
  39. ncbi request reprint Ectopic mTERT expression in mouse embryonic stem cells does not affect differentiation but confers resistance to differentiation- and stress-induced p53-dependent apoptosis
    Ming Kei Lee
    National Cancer Centre, 11, Hospital Drive, Singapore 169610, Republic of Singapore
    J Cell Sci 118:819-29. 2005
    ....
  40. ncbi request reprint Yeast Nhp6A/B and mammalian Hmgb1 facilitate the maintenance of genome stability
    Sabrina Giavara
    The Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Curr Biol 15:68-72. 2005
    ..Taken together, these data indicate that Nhp6A/B and Hmgb1 protect DNA from damaging agents and thus guard against the generation of genomic aberrations...
  41. ncbi request reprint The difference in LET and ion species dependence for induction of initially measured and non-rejoined chromatin breaks in normal human fibroblasts
    Chizuru Tsuruoka
    Heavy Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4 9 1 Anagawa, Chiba, Japan
    Radiat Res 170:163-71. 2008
    ..This suggests that the LET-dependent structure in the biological effects is reflected in biological consequences of repair processes...