R Manjunatha Kini

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. ncbi request reprint Hemextin AB complex, a unique anticoagulant protein complex from Hemachatus haemachatus (African Ringhals cobra) venom that inhibits clot initiation and factor VIIa activity
    Yajnavalka Banerjee
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore Singapore 117543
    J Biol Chem 280:42601-11. 2005
  2. pmc Metalloproteases Affecting Blood Coagulation, Fibrinolysis and Platelet Aggregation from Snake Venoms: Definition and Nomenclature of Interaction Sites
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, 14 Science Drive 4, National University of Singapore, Singapore 117543, Singapore
    Toxins (Basel) 8:. 2016
  3. doi request reprint Identification and characterisation of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) venom
    Wan Chen
    R Manjunatha Kini, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Block S3, 03 17, Singapore 117543, Singapore, E mail
    Thromb Haemost 112:700-15. 2014
  4. pmc Identification and structural characterization of a new three-finger toxin hemachatoxin from Hemachatus haemachatus venom
    Vallerinteavide Mavelli Girish
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    PLoS ONE 7:e48112. 2012
  5. pmc The venom gland transcriptome of the Desert Massasauga rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea)
    Susanta Pahari
    Center for Post Graduate Studies, Sri Bhagawan Mahaveer Jain College, 18 3, 9th Main, Jayanagar 3rd Block, Bangalore, India
    BMC Mol Biol 8:115. 2007
  6. ncbi request reprint Serine proteases affecting blood coagulation and fibrinolysis from snake venoms
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Pathophysiol Haemost Thromb 34:200-4. 2005
  7. ncbi request reprint Excitement ahead: structure, function and mechanism of snake venom phospholipase A2 enzymes
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    Toxicon 42:827-40. 2003
  8. ncbi request reprint Platelet aggregation and exogenous factors from animal sources
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore 117543
    Curr Drug Targets Cardiovasc Haematol Disord 4:301-25. 2004
  9. ncbi request reprint Molecular moulds with multiple missions: functional sites in three-finger toxins
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Clin Exp Pharmacol Physiol 29:815-22. 2002
  10. doi request reprint Nucleotide sequence determines the accelerated rate of point mutations
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    Toxicon 56:295-304. 2010

Collaborators

Detail Information

Publications91

  1. ncbi request reprint Hemextin AB complex, a unique anticoagulant protein complex from Hemachatus haemachatus (African Ringhals cobra) venom that inhibits clot initiation and factor VIIa activity
    Yajnavalka Banerjee
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore Singapore 117543
    J Biol Chem 280:42601-11. 2005
    ..Molecular interactions of the hemextin AB complex with FVIIa/TF.FVIIa will provide a new paradigm in the search for anticoagulants that inhibit the initiation of blood coagulation...
  2. pmc Metalloproteases Affecting Blood Coagulation, Fibrinolysis and Platelet Aggregation from Snake Venoms: Definition and Nomenclature of Interaction Sites
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, 14 Science Drive 4, National University of Singapore, Singapore 117543, Singapore
    Toxins (Basel) 8:. 2016
    ..This review provides a ready reference for metalloproteases that interfere in blood coagulation, fibrinolysis and platelet aggregation...
  3. doi request reprint Identification and characterisation of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) venom
    Wan Chen
    R Manjunatha Kini, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Block S3, 03 17, Singapore 117543, Singapore, E mail
    Thromb Haemost 112:700-15. 2014
    ..This is the first report on the anticoagulant activity ofβ-bungarotoxins and these results expand on the existing catalogue of haemostatically active snake venom proteins...
  4. pmc Identification and structural characterization of a new three-finger toxin hemachatoxin from Hemachatus haemachatus venom
    Vallerinteavide Mavelli Girish
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    PLoS ONE 7:e48112. 2012
    ..Based on the analysis, we predict hemachatoxin to be cardiotoxic/cytotoxic and our future experiments will be directed to characterize the activity of hemachatoxin...
  5. pmc The venom gland transcriptome of the Desert Massasauga rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea)
    Susanta Pahari
    Center for Post Graduate Studies, Sri Bhagawan Mahaveer Jain College, 18 3, 9th Main, Jayanagar 3rd Block, Bangalore, India
    BMC Mol Biol 8:115. 2007
    ..Global cataloguing of the venom transcriptome facilitates the identification of new families of toxins as well as helps in understanding the evolution of venom proteomes...
  6. ncbi request reprint Serine proteases affecting blood coagulation and fibrinolysis from snake venoms
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Pathophysiol Haemost Thromb 34:200-4. 2005
    ..These serine proteases serve as tools to study molecular details in the activation of specific factors involved in coagulation and fibrinolytic cascades and are useful in treating various thrombotic and hemostatic conditions...
  7. ncbi request reprint Excitement ahead: structure, function and mechanism of snake venom phospholipase A2 enzymes
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    Toxicon 42:827-40. 2003
    ..Thus research in this field will provide a lot of exciting opportunities...
  8. ncbi request reprint Platelet aggregation and exogenous factors from animal sources
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore 117543
    Curr Drug Targets Cardiovasc Haematol Disord 4:301-25. 2004
    ..Further studies in this area will help in the development of novel strategies for treating cardiovascular and hematological disorders...
  9. ncbi request reprint Molecular moulds with multiple missions: functional sites in three-finger toxins
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Clin Exp Pharmacol Physiol 29:815-22. 2002
    ..4. Thus, the structure-function relationships of this group of toxins are complicated and challenging. 5. Studies have shown that the functional sites in these "sibling" toxins are located on various segments of the molecular surface...
  10. doi request reprint Nucleotide sequence determines the accelerated rate of point mutations
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    Toxicon 56:295-304. 2010
    ..These findings show that mutation rates of proteins, and hence of the organisms, are DNA sequence-dependent and thus provide a proximate mechanism of evolution at the molecular level...
  11. ncbi request reprint The intriguing world of prothrombin activators from snake venom
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    Toxicon 45:1133-45. 2005
    ..This review provides a detailed description of the current knowledge on all prothrombin activators and highlights several intriguing questions that are yet to be answered...
  12. ncbi request reprint Structure-function relationships and mechanism of anticoagulant phospholipase A2 enzymes from snake venoms
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    Toxicon 45:1147-61. 2005
    ..Thus, these studies strongly support the target model which suggests that protein-protein interaction rather than protein-phospholipid interaction determines the pharmacological specificity of PLA(2) enzymes...
  13. ncbi request reprint Procoagulant proteins from snake venoms
    R M Kini
    Department of Biological Sciences, Faculty of Science, Block S2 Room 04 11, 14 Science Drive, National University of Singapore, Singapore 117543
    Haemostasis 31:218-24. 2001
    ..Their structural studies should provide us insight into prothrombinase complex formation. Here, we present an overview of snake venom procoagulant factors...
  14. doi request reprint Toxins in thrombosis and haemostasis: potential beyond imagination
    R M Kini
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    J Thromb Haemost 9:195-208. 2011
    ..Based on the current status, I have attempted to identify future potential and prospects in this area of research...
  15. pmc Anticoagulant proteins from snake venoms: structure, function and mechanism
    R Manjunatha Kini
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore
    Biochem J 397:377-87. 2006
    ..This review describes the details of the structure, mechanism and structure-function relationships of anticoagulant proteins from snake venoms...
  16. doi request reprint Structure, function and evolution of three-finger toxins: mini proteins with multiple targets
    R Manjunatha Kini
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    Toxicon 56:855-67. 2010
    ..In this review, we describe the structural and functional diversity, structure-function relationships and evolution of this group of snake venom toxins...
  17. doi request reprint Irditoxin, a novel covalently linked heterodimeric three-finger toxin with high taxon-specific neurotoxicity
    Joanna Pawlak
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Science Dr 4, Singapore 117543
    FASEB J 23:534-45. 2009
    ..Covalently linked heterodimeric three-finger toxins found in colubrid venoms constitute a new class of venom peptides, which may be a useful source of new neurobiology probes and therapeutic leads...
  18. pmc Structural and functional characterization of a novel homodimeric three-finger neurotoxin from the venom of Ophiophagus hannah (king cobra)
    Amrita Roy
    Department of Biological Sciences, National University of Singapore, Singapore
    J Biol Chem 285:8302-15. 2010
    ..This is the first report of a dimeric short-chain alpha-neurotoxin interacting with neuronal alpha(7)-nAChRs as well as the first homodimeric three-finger toxin to interact with muscle nAChRs...
  19. pmc Crystal structure of thrombin in complex with S-variegin: insights of a novel mechanism of inhibition and design of tunable thrombin inhibitors
    Cho Yeow Koh
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e26367. 2011
    ..In vivo antithrombotic effects of the variegin variants correlate well with their in vitro affinities for thrombin. Our results encourage that variegin and the variants show strong potential for the development of tunable anticoagulants...
  20. pmc Role of accelerated segment switch in exons to alter targeting (ASSET) in the molecular evolution of snake venom proteins
    Robin Doley
    Department of Biological Sciences, National University of Singapore, Singapore
    BMC Evol Biol 9:146. 2009
    ..In this phenomenon, short sequences in exons are radically changed to unrelated sequences and hence affect the folding and functional properties of the toxins...
  21. pmc Accelerated exchange of exon segments in Viperid three-finger toxin genes (Sistrurus catenatus edwardsii; Desert Massasauga)
    Robin Doley
    Protein Science Laboratory, Department of Biological Sciences, National University of Singapore, 117543, Singapore
    BMC Evol Biol 8:196. 2008
    ..Venom proteins are encoded by multigene families that have evolved through a process of gene duplication followed by accelerated evolution in the protein coding region...
  22. doi request reprint Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix
    Rajamani Lakshminarayanan
    Department of Chemistry, Faculty of Science, 3 Science Drive 3, National University of Singapore, Singapore 117543
    J Am Chem Soc 130:4660-8. 2008
    ..The results demonstrate the adaptability of an extracellular matrix protein to perform multiple tasks: polymorph discrimination and protection of the contents of the egg against bacterial invasion...
  23. pmc Expression pattern of three-finger toxin and phospholipase A2 genes in the venom glands of two sea snakes, Lapemis curtus and Acalyptophis peronii: comparison of evolution of these toxins in land snakes, sea kraits and sea snakes
    Susanta Pahari
    Protein Science and Conservation Ecology Laboratories, Department of Biological Sciences, National University of Singapore, 117543, Singapore
    BMC Evol Biol 7:175. 2007
    ..It is therefore interesting to examine the evolution of toxins in sea snake venoms compared to that of land snakes...
  24. pmc Unusual accelerated rate of deletions and insertions in toxin genes in the venom glands of the pygmy copperhead (Austrelaps labialis) from Kangaroo island
    Robin Doley
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    BMC Evol Biol 8:70. 2008
    ....
  25. doi request reprint Identification of novel proteins from the venom of a cryptic snake Drysdalia coronoides by a combined transcriptomics and proteomics approach
    Shifali Tiwari Chatrath
    Department of Biological Sciences, National University of Singapore, Singapore
    J Proteome Res 10:739-50. 2011
    ..We also identified three novel proteins belonging to the three-finger toxin superfamily...
  26. doi request reprint Molecular diversity of anticoagulants from haematophagous animals
    Cho Yeow Koh
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Science Drive 4, Singapore 117543, Singapore
    Thromb Haemost 102:437-53. 2009
    ..In this review, we will provide an overview on the structure, function and mechanism of the exogenous anticoagulants from haematophagous animals and rationalise their molecular diversity...
  27. ncbi request reprint Mimicking the function of eggshell matrix proteins: the role of multiplets of charged amino acid residues and self-assembly of peptides in biomineralization
    Parayil Kumaran Ajikumar
    Department of Chemistry and Singapore MIT Alliance, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
    Angew Chem Int Ed Engl 44:5476-9. 2005
  28. doi request reprint Non-enzymatic proteins from snake venoms: a gold mine of pharmacological tools and drug leads
    Ryan J R McCleary
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    Toxicon 62:56-74. 2013
    ..We expect that this review will provide impetus for even further exciting research on novel toxins yet to be discovered...
  29. pmc Antimicrobial activity of omwaprin, a new member of the waprin family of snake venom proteins
    Dileep G Nair
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    Biochem J 402:93-104. 2007
    ....
  30. ncbi request reprint Hemextin AB complex--a snake venom anticoagulant protein complex that inhibits factor VIIa activity
    Yajnavalka Banerjee
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Pathophysiol Haemost Thromb 34:184-7. 2005
    ..Molecular interactions of hemextin AB complex with FVIIa/tissue factor-FVIIa may provide a new paradigm in the search for anticoagulants inhibiting the initiation of blood coagulation...
  31. ncbi request reprint Eggs-only diet: its implications for the toxin profile changes and ecology of the marbled sea snake (Aipysurus eydouxii)
    Min Li
    Department of Biological Science, Faculty of Science, National University of Singapore, Singapore 119260
    J Mol Evol 60:81-9. 2005
    ..It is interesting to note that a potent venom was not maintained for use in defense, thus reinforcing that the primary use of snake venom is for prey capture...
  32. doi request reprint Identification of a novel family of snake venom proteins Veficolins from Cerberus rynchops using a venom gland transcriptomics and proteomics approach
    G OmPraba
    Protein Sciences Laboratory, Department of Biological Sciences, 14 Science Drive 4, National University of Singapore, Singapore
    J Proteome Res 9:1882-93. 2010
    ..Overall, our combined approach of transcriptomics and proteomics revealed that C. rynchops venom is among the least complex snake venom characterized to date despite the presence of a new family of snake venom proteins...
  33. pmc Group D prothrombin activators from snake venom are structural homologues of mammalian blood coagulation factor Xa
    Veena S Rao
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 119260
    Biochem J 369:635-42. 2003
    ..The glycosylation on the heavy chain is a large carbohydrate moiety adjacent to the active-site pocket. Overall, hopsarin D is structurally and functionally similar to mammalian coagulation FXa...
  34. ncbi request reprint Peptides derived from human decorin leucine-rich repeat 5 inhibit angiogenesis
    Konerirajapuram N Sulochana
    Department of Biological Sciences, National University of Singapore, Singapore
    J Biol Chem 280:27935-48. 2005
    ..Peptide LRR5 and its derivatives are therefore novel angiogenesis inhibitors that may serve as prototypes for further development into anti-angiogenic drugs...
  35. pmc Isthmin is a novel secreted angiogenesis inhibitor that inhibits tumour growth in mice
    Wei Xiang
    Department of Biological Sciences, National University of Singapore, Singapore
    J Cell Mol Med 15:359-74. 2011
    ..Our results demonstrate that ISM is a novel endogenous angiogenesis inhibitor with functions likely in physiological as well as pathological angiogenesis...
  36. ncbi request reprint The nonenzymatic subunit of pseutarin C, a prothrombin activator from eastern brown snake (Pseudonaja textilis) venom, shows structural similarity to mammalian coagulation factor V
    Veena S Rao
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Republic of Singapore
    Blood 102:1347-54. 2003
    ..Thus, our data demonstrate that the nonenzymatic subunit of group C prothrombin activators is structurally similar to mammalian FV...
  37. doi request reprint Application of isothermal titration calorimetry and column chromatography for identification of biomolecular targets
    Xingding Zhou
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Nat Protoc 6:158-65. 2011
    ..With this protocol, it takes approximately 3-5 d to identify the target protein from a mixture...
  38. doi request reprint The recruitment of blood coagulation factor X into snake venom gland as a toxin: the role of promoter cis-elements in its expression
    Shiyang Kwong
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    Thromb Haemost 102:469-78. 2009
    ..VERSE is primarily responsible for the increase of trocarin D expression. The insertions/deletions within trocarin D intron 1 need to be characterised for their role in tissue-specific and inducible expression of trocarin D...
  39. ncbi request reprint Variegin, a novel fast and tight binding thrombin inhibitor from the tropical bont tick
    Cho Yeow Koh
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    J Biol Chem 282:29101-13. 2007
    ..However, compared with hirulog, variegin is a more potent inhibitor, and its inhibitory activity is largely retained after cleavage by thrombin...
  40. ncbi request reprint Denmotoxin, a three-finger toxin from the colubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity
    Joanna Pawlak
    Department of Biological Sciences, Faculty of Science, National University of Singapore
    J Biol Chem 281:29030-41. 2006
    ..Denmotoxin illustrates the relationship between toxin specificity and the primary prey type that constitutes the snake's diet...
  41. ncbi request reprint Autonomic effects of some scorpion venoms and toxins
    Matthew C E Gwee
    Department of Pharmacology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Clin Exp Pharmacol Physiol 29:795-801. 2002
    ..longimanus and H. spinifer do not have such a prejunctional site of action but, instead, the venoms mediate their autonomic effects through direct agonist actions on post-junctional muscarinic M3 cholinoceptors and alpha-adrenoceptors...
  42. ncbi request reprint Ohanin, a novel protein from king cobra venom: its cDNA and genomic organization
    Yuh Fen Pung
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 117543 Singapore
    Gene 371:246-56. 2006
    ..Our results indicate that ohanin and vespryns may have evolved from the same ancestral gene as B30.2 domain...
  43. pmc Biophysical characterization of anticoagulant hemextin AB complex from the venom of snake Hemachatus haemachatus
    Yajnavalka Banerjee
    Department of Biological Sciences, Faculty of Science, and Department of Chemistry, National University of Singapore, Singapore
    Biophys J 93:3963-76. 2007
    ..The two dimers formed under each of these conditions display distinct differences in their apparent molecular diameters and anticoagulant properties. Based on these results, we have proposed a model for this unique anticoagulant complex...
  44. ncbi request reprint Effect of snake venom procoagulants on snake plasma: implications for the coagulation cascade of snakes
    Jeremiah S Joseph
    Department of Biochemistry, Faculty of Medicine, National University of, Singapore, Singapore 119260
    Toxicon 40:175-83. 2002
    ..In contrast, although Notechis and Pseudonaja plasmas were clotted by a Group C activator, they failed to clot upon the addition of factor Xa and Group D activators. The mechanism of this resistance is still elusive...
  45. ncbi request reprint In vitro study of magnesium-calcite biomineralization in the skeletal materials of the seastar Pisaster giganteus
    Subramanyam Gayathri
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
    Chemistry 13:3262-8. 2007
    ....
  46. ncbi request reprint Formation of transient amorphous calcium carbonate precursor in quail eggshell mineralization: an in vitro study
    Rajamani Lakshminarayanan
    Department of Chemistry, NUS Nanoscience and Nanotechnology Initiative, and Department of Biological Sciences, National University of Singapore, Singapore, 3 Science Drive 3, Singapore 117543
    Biomacromolecules 7:3202-9. 2006
    ..Altogether, these observations further demonstrate that this strategy may be common in both vertebrate and invertebrate mineralized structures...
  47. pmc Protein folding determinants: structural features determining alternative disulfide pairing in alpha- and chi/lambda-conotoxins
    Tse Siang Kang
    Protein Science Laboratory, Department of Biological Sciences, National University of Singapore, Singapore
    Biochemistry 46:3338-55. 2007
    ..These results strongly indicate that the first intercysteine loop proline and C-terminal amidation act as conformational switches in alpha- and chi/lambda-conotoxins...
  48. ncbi request reprint Gene structures of trocarin D and coagulation factor X, two functionally diverse prothrombin activators from Australian rough scaled snake
    M Abu Reza
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Pathophysiol Haemost Thromb 34:205-8. 2005
    ..Further studies on the characterization of these two genes particularly the promoter regions are in progress...
  49. ncbi request reprint Effect of C-terminal amidation on folding and disulfide-pairing of alpha-conotoxin ImI
    Tse Siang Kang
    Department of Biological Sciences, National University of Singapore, Singapore
    Angew Chem Int Ed Engl 44:6333-7. 2005
  50. ncbi request reprint Ohanin, a novel protein from king cobra venom, induces hypolocomotion and hyperalgesia in mice
    Yuh Fen Pung
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    J Biol Chem 280:13137-47. 2005
    ..Recombinant ohanin is structurally and functionally similar to native protein as determined by circular dichroism and hot plate assay, suggesting that it will be useful in future structure-function relationship studies...
  51. ncbi request reprint Structure-function relationship of avian eggshell matrix proteins: a comparative study of two major eggshell matrix proteins, ansocalcin and OC-17
    Rajamani Lakshminarayanan
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543
    Biomacromolecules 6:741-51. 2005
    ..To our knowledge, this is the first report on the comparison of properties of homologous eggshell proteins that belong to the same phylogeny...
  52. ncbi request reprint Solution structures of two structural isoforms of CMrVIA chi/lambda-conotoxin
    Tse Siang Kang
    Protein Science Laboratory, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Block S3 03 17 Singapore
    Biomacromolecules 7:2337-46. 2006
    ..These structural data are essential for the understanding of the structure-function activity of chi/lambda-conotoxins, as well as unraveling the folding propensities of these short peptide toxins...
  53. doi request reprint Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis
    Tse Siang Kang
    Department of Pharmacy, National University of Singapore, Singapore
    FEBS J 278:4544-76. 2011
    ..The structures of inhibitor-enzyme complexes provide ideal platforms for the design of potent inhibitors which are useful in the development of prototypes and lead compounds with potential therapeutic applications...
  54. ncbi request reprint Pseutarin C, a prothrombin activator from Pseudonaja textilis venom: its structural and functional similarity to mammalian coagulation factor Xa-Va complex
    Veena S Rao
    Department of Biological Sciences, Faculty of Science, Block S2, 14 Science Drive 4, National University of Singapore, Republic of Singapore
    Thromb Haemost 88:611-9. 2002
    ..Thus pseutarin C is the first venom procoagulant protein that is structurally and functionally similar to mammalian factor Xa-Va complex...
  55. pmc A universal method for fishing target proteins from mixtures of biomolecules using isothermal titration calorimetry
    Xingding Zhou
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    Protein Sci 17:1798-804. 2008
    ..This method has the potential to make a significant advancement in the area of identifying orphan target proteins and inhibitor screening in drug discovery and characterization...
  56. doi request reprint Unique gene organization of colubrid three-finger toxins: complete cDNA and gene sequences of denmotoxin, a bird-specific toxin from colubrid snake Boiga dendrophila (Mangrove Catsnake)
    Joanna Pawlak
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore117543, Singapore
    Biochimie 90:868-77. 2008
    ..It appears that Elapidae snakes have lost the extra second exon after the divergence of the snake families...
  57. ncbi request reprint Beta-cardiotoxin: a new three-finger toxin from Ophiophagus hannah (king cobra) venom with beta-blocker activity
    Nandhakishore Rajagopalan
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Science Dr 4, Singapore 117543
    FASEB J 21:3685-95. 2007
    ..3 and 2.3 microM toward beta1 and beta2 subtypes, respectively, to bring about its effect, and hence, it was named as beta-cardiotoxin. This is the first report of a natural exogenous beta-blocker...
  58. pmc Ringhalexin from Hemachatus haemachatus: A novel inhibitor of extrinsic tenase complex
    Bhaskar Barnwal
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 119260, Singapore
    Sci Rep 6:25935. 2016
    ..95 Å resolution. This study for the first time reports the structure of an anticoagulant three-finger toxin. Thus, ringhalexin is a potent inhibitor of the FX activation by extrinsic tenase complex and a weak, irreversible neurotoxin. ..
  59. pmc Crystallization and preliminary X-ray diffraction analysis of hemextin A: a unique anticoagulant protein from Hemachatus haemachatus venom
    Yajnavalka Banerjee
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:701-3. 2007
    ..The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 49.27, b = 49.51, c = 57.87 A and two molecules in the asymmetric unit. They diffracted to 1.5 A resolution at beamline X25 at BNL...
  60. doi request reprint Structural determinants in protein folding: a single conserved hydrophobic residue determines folding of EGF domains
    A S Angie Ng
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    ACS Chem Biol 8:161-9. 2013
    ..Taken together, our results for the first time demonstrate that a single conserved hydrophobic residue acts as the key determinant in the folding of EGF domains...
  61. doi request reprint From snake venom toxins to therapeutics--cardiovascular examples
    Cho Yeow Koh
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 117543 Singapore, Singapore
    Toxicon 59:497-506. 2012
    ..We also discuss the successes and failures as an update to the advances in the field...
  62. pmc Augmenter of liver regeneration (alr) promotes liver outgrowth during zebrafish hepatogenesis
    Yan Li
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 7:e30835. 2012
    ..The dose-dependent and partial suppression of alr expression through MO-mediated knockdown allows the identification of its late developmental role in vertebrate liver organogenesis...
  63. ncbi request reprint Isolation of a neurotoxin (alpha-colubritoxin) from a nonvenomous colubrid: evidence for early origin of venom in snakes
    Bryan G Fry
    Department of Biological Sciences, Faculty of Science, National University of Singapore, 119260 Singapore
    J Mol Evol 57:446-52. 2003
    ..These results support the role of venom as a key evolutionary innovation in the early diversification of advanced snakes and provide evidence that forces a fundamental rethink of the very concept of nonvenomous snake...
  64. pmc Interrogating the Venom of the Viperid Snake Sistrurus catenatus edwardsii by a Combined Approach of Electrospray and MALDI Mass Spectrometry
    Alex Chapeaurouge
    Laboratorio de Toxinologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, 21045 900, Brazil Department of Biological Sciences, 14 Science Drive 4, National University of Singapore, Singapore, 117543, Singapore
    PLoS ONE 10:e0092091. 2015
    ....
  65. pmc Structural characterization of myotoxic ecarpholin S from Echis carinatus venom
    Xingding Zhou
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
    Biophys J 95:3366-80. 2008
    ..This study provides significant structural and functional insights into the myotoxic activity of ecarpholin S and, in general, of non-Asp(49)-PLA(2) enzymes...
  66. ncbi request reprint Characterization of the interaction of wheat HMGa with linear and four-way junction DNAs
    Wensheng Zhang
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543
    Biochemistry 42:6596-607. 2003
    ..These differences could be due to the rigidity of the DNA and the characters of the AT hook regions in the mutants...
  67. ncbi request reprint Hypotensive agents from snake venoms
    Roy Joseph
    Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543
    Curr Drug Targets Cardiovasc Haematol Disord 4:437-59. 2004
    ..Therefore these and other related snake venom proteins hold great promise in the future understanding and treatment of cardiovascular diseases...
  68. ncbi request reprint Putting the brakes on snake venom evolution: the unique molecular evolutionary patterns of Aipysurus eydouxii (Marbled sea snake) phospholipase A2 toxins
    Min Li
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    Mol Biol Evol 22:934-41. 2005
    ..This is the first case of decelerated evolution of toxins in snake venom...
  69. doi request reprint Complement C3 isoforms in Austrelaps superbus
    Syed Rehana
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    Toxicon 51:864-81. 2008
    ..These genes offer a system to identify the tissue-specific regulatory proteins that are responsible for the constitutive expression of complement C3 in liver and inducible expression of AVF genes in the venom gland of A. superbus...
  70. ncbi request reprint Accelerated evolution and molecular surface of venom phospholipase A2 enzymes
    R M Kini
    Bioscience Centre, Faculty of Science, National University of Singapore, 10, Kent Ridge Crescent, Singapore 119260
    J Mol Evol 48:125-32. 1999
    ..Thus natural substitutions in PLA2 enzymes, in contrast to popular belief, are not random substitutions but appear to be directed toward modifying the molecular surface...
  71. pmc Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags
    Ang Swee Siang
    Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Road, Singapore 117546, Singapore
    BMC Mol Biol 11:24. 2010
    ..Although the venoms of most species of the snake genus Bungarus have been well characterized, a detailed compositional analysis of B. flaviceps is currently lacking...
  72. ncbi request reprint Candoxin, a novel toxin from Bungarus candidus, is a reversible antagonist of muscle (alphabetagammadelta ) but a poorly reversible antagonist of neuronal alpha 7 nicotinic acetylcholine receptors
    Selvanayagam Nirthanan
    Venom and Toxin Research Programme, Faculty of Medicine, National University of Singapore, Singapore 119260, Republic of Singapore
    J Biol Chem 277:17811-20. 2002
    ..Candoxin is therefore a novel toxin that shares a common scaffold with long chain alpha-neurotoxins but possibly utilizes additional functional determinants that assist in recognizing neuronal alpha7 receptors...
  73. ncbi request reprint kappa-Hefutoxin1, a novel toxin from the scorpion Heterometrus fulvipes with unique structure and function. Importance of the functional diad in potassium channel selectivity
    Kellathur N Srinivasan
    Venom and Toxin Research Programme, Faculty of Medicine, National University of Singapore, 4 Medical Dr, Singapore 117597, Singapore
    J Biol Chem 277:30040-7. 2002
    ..Alanine mutants (Y5A, K19A, and Y5A/K19A) failed to block the channels, indicating the importance of the functional diad...
  74. ncbi request reprint Purification and properties of three new phospholipase A2 isoenzymes from Micropechis ikaheka venom
    R Gao
    Venom and Toxin Research Programme, Department of Anatomy, National University of Singapore, Singapore
    Biochim Biophys Acta 1545:30-40. 2001
    ..On the other hand, no direct correlation between their insulin secretion stimulating effect and enzymatic activity was observed, suggesting that they may stimulate insulin secretion through a non-enzymatic mechanism...
  75. ncbi request reprint Snake venom prothrombin activators homologous to blood coagulation factor Xa
    J S Joseph
    Department of Biochemistry, Faculty of Medicine, National University of Singapore, 14 Science Drive, Singapore 117543
    Haemostasis 31:234-40. 2001
    ..Interestingly, the EPR-1 recognition site is distinctly different in trocarin, the functional consequences of which are being investigated...
  76. ncbi request reprint Purification, properties, and amino acid sequence of a hemoglobinuria-inducing phospholipase A(2), MiPLA-1, from Micropechis ikaheka venom
    R Gao
    Department of Anatomy, Faculty of Medicine, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260, Singapore
    Arch Biochem Biophys 369:181-92. 1999
    ..Two short segments flanked by proline brackets are found in the sequence of MiPLA-1. These segments are on the surface of the molecule and hence may be involved in protein-protein recognition...
  77. pmc Neuromuscular effects of candoxin, a novel toxin from the venom of the Malayan krait (Bungarus candidus)
    S Nirthanan
    Venom and Toxin Research Programme, Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore
    Br J Pharmacol 139:832-44. 2003
    ..In contrast, curaremimetic alpha-neurotoxins have been reported to antagonize both binding sites with equal affinity...
  78. ncbi request reprint Structure of two genes encoding parallel prothrombin activators in Tropidechis carinatus snake: gene duplication and recruitment of factor X gene to the venom gland
    M A Reza
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    J Thromb Haemost 5:117-26. 2007
    ..For example, in Tropidechis carinatus, trocarin D exists in the venom and acts as toxin, whereas coagulation factor (F) X exists in plasma and plays a role in hemostasis...
  79. doi request reprint Protein complexes in snake venom
    R Doley
    Protein Science Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Cell Mol Life Sci 66:2851-71. 2009
    ..Here, we describe the structure and function of various protein complexes of snake venoms and their role in snake venom toxicity...
  80. ncbi request reprint Snake venom prothrombin activators similar to blood coagulation factor Xa
    Jeremiah S Joseph
    The Scripps Research Institute, La Jolla, California 92037, USA
    Curr Drug Targets Cardiovasc Haematol Disord 4:397-416. 2004
    ....
  81. pmc Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix
    Rajamani Lakshminarayanan
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117 543
    Proc Natl Acad Sci U S A 99:5155-9. 2002
    ..The polycrystalline aggregates obtained at higher concentration of ansocalcin appears to be similar to the crystals observed at the mamillary layer of the eggshell...
  82. ncbi request reprint Trocarin, a blood coagulation factor Xa homologue from snake venom, causes inflammation and mitogenesis
    Jeremiah S Joseph
    Department of Biochemistry, Faculty of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597
    Toxicon 42:769-76. 2003
    ..Hence trocarin, being closely related to FXa, has similar non-hemostatic functions in mediating inflammation and mitogenesis, yet appears to act by distinctly different mechanisms...
  83. ncbi request reprint Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus) that targets small conductance Ca2+-activated K+ channels and afterhyperpolarization currents in central neurons
    Paola Pedarzani
    Department of Physiology, University College London, London WC1E 6BT, United Kingdom
    J Biol Chem 277:46101-9. 2002
    ..Tamapin will facilitate the characterization of the subunit composition of native SK channels and help determine their involvement in electrical and biochemical signaling...
  84. ncbi request reprint Isolation and pharmacological characterization of cannitoxin, a presynaptic neurotoxin from the venom of the Papuan Taipan (Oxyuranus scutellatus canni)
    Sanjaya Kuruppu
    Monash Venom Group, Department of Pharmacology, Monash University, Victoria, Australia
    J Pharmacol Exp Ther 315:1196-202. 2005
    ..Thus, cannitoxin is similar in structure and pharmacology to taipoxin, which has been isolated from the closely related Australian species O. scutellatus scutellatus (coastal taipan)...
  85. ncbi request reprint Analysis of the peanut agglutinin molten globule-like intermediate by limited proteolysis
    Nivedita Mitra
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, Karnataka, India
    Biochim Biophys Acta 1725:283-9. 2005
    ..This effect is significantly reduced by the presence of 90% saturating lactose, as deduced by a reduction in cleavage propensity. This is also supported by the gain in the tertiary structure as observed by near-UV CD...
  86. ncbi request reprint Identification of a novel family of proteins in snake venoms. Purification and structural characterization of nawaprin from Naja nigricollis snake venom
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 278:40097-104. 2003
    ..The amino acid residues in these important regions in nawaprin are different from those in elafin, suggesting that nawaprin is not an elastase-specific inhibitor and therefore has a different function in the snake venom...
  87. ncbi request reprint Pharmacological characterisation of a neurotoxin from the venom of Boiga dendrophila (mangrove catsnake)
    Natalie G Lumsden
    Monash Venom Group, Department of Pharmacology, Monash University, Clayton VIC 3800, Australia
    Toxicon 45:329-34. 2005
    ..This is the first report of such activity for a toxin isolated from snake venom and reinforces the largely untapped potential of colubrid venoms...
  88. ncbi request reprint Isolation and characterization of rufoxin, a novel protein exhibiting neurotoxicity from venom of the psammophiine, Rhamphiophis oxyrhynchus (Rufous beaked snake)
    Natalie G Lumsden
    Institute for Molecular Bioscience, University of Queensland, St Lucia, QLD 4072, Australia
    Neuropharmacology 52:1065-70. 2007
    ..Rufoxin lacks NH(2)-terminal sequence homology to previously identified snake venom toxins. This work indicates a wider distribution of neurotoxins across the advanced snake superfamily than previously described...
  89. pmc Isolation and pharmacological characterization of a phospholipase A2 myotoxin from the venom of the Irian Jayan death adder (Acanthophis rugosus)
    Janith C Wickramaratna
    Monash Venom Group, Department of Pharmacology, P O Box 13E, Monash University, Victoria 3800, Australia
    Br J Pharmacol 138:333-42. 2003
    ..1 Hz, 0.2 ms, supramaximal V) stimulated CBCNM preparation. 6. In conclusion, clinicians may need to be mindful of possible myotoxicity following death adder envenomation in Irian Jaya...
  90. ncbi request reprint Purification, characterization, and in vitro mineralization studies of a novel goose eggshell matrix protein, ansocalcin
    Rajamani Lakshminarayanan
    Department of Chemistry, National University of Singapore and the Department of Biological Sciences, National University of Singapore, Singapore 117 543
    J Biol Chem 278:2928-36. 2003
    ..Similar aggregation of calcite crystals was also observed when crystallizations were performed in the presence of whole goose eggshell extract. These results show that ansocalcin plays a significant role in goose eggshell calcification...
  91. ncbi request reprint Analysis of Colubroidea snake venoms by liquid chromatography with mass spectrometry: evolutionary and toxinological implications
    Bryan G Fry
    Department of Pharmacology, University of Melbourne, Parkville, VIC 3010, Australia
    Rapid Commun Mass Spectrom 17:2047-62. 2003
    ..The data will also be useful in biodiscovery...