Zhiyuan Gong

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. pmc Development of a convenient in vivo hepatotoxin assay using a transgenic zebrafish line with liver-specific DsRed expression
    Xiaoyan Zhang
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 9:e91874. 2014
  2. ncbi request reprint Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8
    Zhiyuan Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 223:204-15. 2002
  3. ncbi request reprint Green fluorescent protein (GFP) transgenic fish and their applications
    Z Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Genetica 111:213-25. 2001
  4. ncbi request reprint Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle
    Zhiyuan Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Biochem Biophys Res Commun 308:58-63. 2003
  5. pmc Molecular conservation of estrogen-response associated with cell cycle regulation, hormonal carcinogenesis and cancer in zebrafish and human cancer cell lines
    Siew Hong Lam
    Genome Institute of Singapore, 02 01Genome, 60 Biopolis Street, 138672 Singapore
    BMC Med Genomics 4:41. 2011
  6. pmc Morphological and molecular evidence for functional organization along the rostrocaudal axis of the adult zebrafish intestine
    Zhengyuan Wang
    Computation and Systems Biology, Singapore MIT Alliance 117543, Singapore
    BMC Genomics 11:392. 2010
  7. pmc Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis
    Leda Mirbahai
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Genomics 12:3. 2011
  8. pmc Wnt signaling is required for early development of zebrafish swimbladder
    Ao Yin
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e18431. 2011
  9. doi request reprint The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development
    Svitlana Korzh
    Department of Biological Sciences, National University of Singapore
    Dev Biol 359:262-76. 2011
  10. pmc A transgenic zebrafish liver tumor model with inducible Myc expression reveals conserved Myc signatures with mammalian liver tumors
    Zhen Li
    Department of Biological Sciences, National University of Singapore, Singapore
    Dis Model Mech 6:414-23. 2013

Collaborators

Detail Information

Publications84

  1. pmc Development of a convenient in vivo hepatotoxin assay using a transgenic zebrafish line with liver-specific DsRed expression
    Xiaoyan Zhang
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 9:e91874. 2014
    ..Thus, the LiPan transgenic fry provide a rapid and convenient in vivo hepatotoxicity assay that should be applicable to high-throughput hepatotoxicity test in drug screening as well as in biomonitoring environmental toxicants. ..
  2. ncbi request reprint Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8
    Zhiyuan Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 223:204-15. 2002
    ....
  3. ncbi request reprint Green fluorescent protein (GFP) transgenic fish and their applications
    Z Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Genetica 111:213-25. 2001
    ..Efforts are also being made in our laboratory to turn GFP transgenic fish into biomonitoring organisms for surveillance of environmental pollution...
  4. ncbi request reprint Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle
    Zhiyuan Gong
    Department of Biological Sciences, National University of Singapore, Singapore
    Biochem Biophys Res Commun 308:58-63. 2003
    ..Furthermore, compared to the wild-type fish, these fluorescent transgenic fish have no advantage in survival and reproduction...
  5. pmc Molecular conservation of estrogen-response associated with cell cycle regulation, hormonal carcinogenesis and cancer in zebrafish and human cancer cell lines
    Siew Hong Lam
    Genome Institute of Singapore, 02 01Genome, 60 Biopolis Street, 138672 Singapore
    BMC Med Genomics 4:41. 2011
    ..In order to determine the potential of zebrafish model for estrogen-related cancer research, we investigated the molecular conservation of estrogen responses operating in both zebrafish and human cancer cell lines...
  6. pmc Morphological and molecular evidence for functional organization along the rostrocaudal axis of the adult zebrafish intestine
    Zhengyuan Wang
    Computation and Systems Biology, Singapore MIT Alliance 117543, Singapore
    BMC Genomics 11:392. 2010
    ..Thus, a systematic structural and functional characterization of the zebrafish intestine is desirable for future studies of the digestive tract and the intestinal biology and development...
  7. pmc Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis
    Leda Mirbahai
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Genomics 12:3. 2011
    ..The zebrafish is increasingly employed as a human disease model; however there is a lack of information on DNA methylation in zebrafish and during fish tumorigenesis...
  8. pmc Wnt signaling is required for early development of zebrafish swimbladder
    Ao Yin
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e18431. 2011
    ..To investigate the potential conservation of signaling events in early development of the lung and swimbladder, we wish to address the question whether Wnt signaling plays a role in swimbladder development...
  9. doi request reprint The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development
    Svitlana Korzh
    Department of Biological Sciences, National University of Singapore
    Dev Biol 359:262-76. 2011
    ..These findings contribute to the understanding of epithelial-mesenchymal interactions and highlight an interaction between Hh and Fgf signaling pathways during esophagus and swimbladder development...
  10. pmc A transgenic zebrafish liver tumor model with inducible Myc expression reveals conserved Myc signatures with mammalian liver tumors
    Zhen Li
    Department of Biological Sciences, National University of Singapore, Singapore
    Dis Model Mech 6:414-23. 2013
    ..Thus, our zebrafish model demonstrated the conserved role of Myc in promoting hepatocarcinogenesis in all vertebrate species...
  11. ncbi request reprint Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio
    Hai Wang
    Department of Biological Sciences, National University of Singapore, Singapore 119260, Singapore
    Gene 356:91-100. 2005
    ..In situ hybridization experiments confirmed that the extrahepatic expression was actually in adipocytes associated with several organs such as the intestine, ovary, and E2-induced testis...
  12. ncbi request reprint Analyses of pancreas development by generation of gfp transgenic zebrafish using an exocrine pancreas-specific elastaseA gene promoter
    Haiyan Wan
    Department of Biological Sciences, National University of Singapore, Singapore
    Exp Cell Res 312:1526-39. 2006
    ..Thus, our works demonstrated the new transgenic line provided a useful experimental tool in analyzing exocrine pancreas development...
  13. ncbi request reprint Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment
    Zhiyuan Ke
    Department of Biological Sciences, National University of Singapore, Singapore
    Mol Cell Neurosci 37:388-401. 2008
    ..Gli2b acts in cell proliferation and plays an early role in the hindbrain within a regulatory cascade involving Notch and Ngn1, as well as a role as specific activator in rhombomere 4...
  14. pmc Localized rbp4 expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development
    Zhen Li
    Department of Biological Sciences, National University of Singapore, Singapore
    BMC Dev Biol 7:117. 2007
    ....
  15. ncbi request reprint Faithful expression of living color reporter genes in transgenic medaka under two tissue-specific zebrafish promoters
    Zhiqiang Zeng
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 234:387-92. 2005
    ..Therefore, the two zebrafish promoters faithfully function in a heterologous fish species, and it is likely that the mechanisms of tissue-specific expression are largely conserved among fish species...
  16. doi request reprint DTA-mediated targeted ablation revealed differential interdependence of endocrine cell lineages in early development of zebrafish pancreas
    Zhen Li
    Department of Biological Sciences, National University of Singapore, 119260 Singapore, Singapore
    Differentiation 78:241-52. 2009
    ..In contrast, the development of delta-cells was independent of beta-cells...
  17. pmc A high level of liver-specific expression of oncogenic Kras(V12) drives robust liver tumorigenesis in transgenic zebrafish
    Anh Tuan Nguyen
    Department of Biological Sciences, National University of Singapore, Singapore, 117543
    Dis Model Mech 4:801-13. 2011
    ..These observations indicate the validity of our transgenic zebrafish to model human liver cancer, and this model might act as a useful platform for drug screening and identifying new therapeutic targets...
  18. doi request reprint Generation of oocyte-specifically expressed cre transgenic zebrafish for female germline excision of loxP-flanked transgene
    Xingjun Liu
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 237:2955-62. 2008
    ..Furthermore, after crossing with another loxP transgenic line, Tg(EF:loxP-mCherry-loxP-egfp), we confirmed that our cre line was capable of activating a loxP-blocked EGFP reporter gene by both maternal and oocyte-expressed Cre...
  19. ncbi request reprint Recapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter
    Bensheng Ju
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 227:14-26. 2003
    ..The mylz2-gfp transgene was also transferred into two zebrafish mutants, spadetail and chordino, and several novel phenotypes in muscle development in these mutants were discovered...
  20. pmc The role of vasculature and blood circulation in zebrafish swimbladder development
    Cecilia Lanny Winata
    Department of Biological Sciences, National University of Singapore, Singapore
    BMC Dev Biol 10:3. 2010
    ..As the swimbladder is lined with an intricate network of blood capillaries, it is of interest to investigate the role of the vascular system during early development of swimbladder...
  21. ncbi request reprint A novel zebrafish kelchlike gene klhl and its human ortholog KLHL display conserved expression patterns in skeletal and cardiac muscles
    Yi Lian Wu
    Department of Biological Sciences, National University of Singapore, Block S2 05 17, 14 Science Drive 4, 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Gene 338:75-83. 2004
    ..The expression pattern of klhl, as well as the presence of the kelch repeats indicates a possible role for Klhl in the organization of striated muscle cytoarchitecture...
  22. doi request reprint Perturbation of zebrafish swimbladder development by enhancing Wnt signaling in Wif1 morphants
    Ao Yin
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    Biochim Biophys Acta 1823:236-44. 2012
    ..Our works established the importance of proper level of Wnt signaling for normal development of swimbladder in zebrafish...
  23. pmc RNA-sequencing analysis of TCDD-induced responses in zebrafish liver reveals high relatedness to in vivo mammalian models and conserved biological pathways
    Zhi Hua Li
    Department of Biological Sciences, National University of Singapore, Singapore Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China
    PLoS ONE 8:e77292. 2013
    ..The expression profiles associated with TCDD could be useful for monitoring environmental dioxin and dioxin-like contamination. ..
  24. doi request reprint Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish
    Muhammad Farooq
    Department of Biological Sciences, National University of Singapore, 117543, Singapore
    Dev Biol 317:336-53. 2008
    ..These results revealed a novel and specific role of hdac3 in liver development and the distinct functions between hdac1 and hdac3 in zebrafish embryonic development...
  25. doi request reprint Inducible and repressable oncogene-addicted hepatocellular carcinoma in Tet-on xmrk transgenic zebrafish
    Zhen Li
    Department of Biological Sciences, National University of Singapore, Singapore
    J Hepatol 56:419-25. 2012
    ..As a potentially high-throughput and cost-effective experimental model, the zebrafish is increasingly recognized for disease studies. Here, we aim at using the zebrafish to generate a convenient hepatocellular carcinoma model...
  26. ncbi request reprint Transcriptome kinetics of arsenic-induced adaptive response in zebrafish liver
    Siew Hong Lam
    Department of Biological Sciences, National University of Singapore
    Physiol Genomics 27:351-61. 2006
    ....
  27. pmc Toxicogenomic analysis suggests chemical-induced sexual dimorphism in the expression of metabolic genes in zebrafish liver
    Xun Zhang
    NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore
    PLoS ONE 7:e51971. 2012
    ..The ubiquity of sex-dimorphic activities at different biological hierarchies indicate the importance and the need of considering the sex factor in many areas of biological researches, especially in toxicology and pathology...
  28. pmc Toxicogenomic and phenotypic analyses of bisphenol-A early-life exposure toxicity in zebrafish
    Siew Hong Lam
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e28273. 2011
    ..The present study further established zebrafish as a model for toxicogenomic inference of early-life chemical exposure toxicity...
  29. pmc Genome-wide identification of molecular pathways and biomarkers in response to arsenic exposure in zebrafish liver
    Hongyan Xu
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 8:e68737. 2013
    ..Thus, our work represents the first comprehensive investigation of molecular mechanism of asenic toxicity and genome-wide search for potential biomarkers for arsenic exposure. ..
  30. pmc Zebrafish whole-adult-organism chemogenomics for large-scale predictive and discovery chemical biology
    Siew Hong Lam
    Department of Biological Sciences, National University of Singapore, Singapore
    PLoS Genet 4:e1000121. 2008
    ..These findings place zebrafish in a strategic position to bridge the wide gap between cell-based and rodent models in chemogenomics research and applications, especially in preclinical drug discovery and toxicology...
  31. pmc Mercury-induced hepatotoxicity in zebrafish: in vivo mechanistic insights from transcriptome analysis, phenotype anchoring and targeted gene expression validation
    Choong Yong Ung
    Department of Biological Sciences, National University of Singapore, Singapore
    BMC Genomics 11:212. 2010
    ....
  32. pmc An inducible kras(V12) transgenic zebrafish model for liver tumorigenesis and chemical drug screening
    Anh Tuan Nguyen
    Department of Biological Sciences, National University of Singapore, Singapore, 117543
    Dis Model Mech 5:63-72. 2012
    ..Collectively, this mifepristone-inducible and reversible kras(V12) transgenic system offers a novel model for understanding hepatocarcinogenesis and a high-throughput screening platform for anti-cancer drugs...
  33. ncbi request reprint Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter
    Yi Lian Wu
    Department of Biological Sciences, National University of Singapore, Singapore
    Gene 408:85-94. 2008
    ....
  34. ncbi request reprint Expression of a novel zebrafish zinc finger gene, gli2b, is affected in Hedgehog and Notch signaling related mutants during embryonic development
    Zhiyuan Ke
    Department of Biological Sciences, National University of Singapore, Singapore 117604
    Dev Dyn 232:479-86. 2005
    ..In mutants affecting the Hedgehog and Notch signaling pathways, gli2b expression was either disrupted or extended in different regions...
  35. pmc Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafish
    Svetlana Korzh
    Department of Biological Sciences, National University of Singapore, Singapore
    BMC Dev Biol 8:84. 2008
    ..In zebrafish, although it has been found that ECs are not required for liver budding, how and when the spatio-temporal pattern of liver growth is coordinated with ECs remains to be elucidated...
  36. ncbi request reprint Zebrafish atonal homologue zath3 is expressed during neurogenesis in embryonic development
    Xukun Wang
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 227:587-92. 2003
    ..In addition, these mutants also affect expression of zath3 in the neuroretina...
  37. pmc Inverted expression profiles of sex-biased genes in response to toxicant perturbations and diseases
    Choong Yong Ung
    Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore
    PLoS ONE 8:e56668. 2013
    ....
  38. doi request reprint Liver X receptor agonist T0901317 induced liver perturbation in zebrafish: histological, gene set enrichment and expression analyses
    Hendrian Sukardi
    Department of Biological Sciences, National University of Singapore, Singapore
    Biochim Biophys Acta 1820:33-43. 2012
    ..The zebrafish is a new vertebrate model for disease modeling, drug and toxicity screening and will be interesting to test for its potential for LXR-related studies...
  39. pmc Genomewide expression analysis in zebrafish mind bomb alleles with pancreas defects of different severity identifies putative Notch responsive genes
    Ashok Hegde
    Laboratory of Developmental Signalling and Patterning, Institute of Molecular and Cell Biology, A STAR Agency for Science, Technology and Research, Singapore, Singapore
    PLoS ONE 3:e1479. 2008
    ..Zebrafish mind bomb (mib) mutants carry mutations on mib gene, which encodes a RING E3 ligase required for Notch activation via Delta/Jagged ubiquitylation and internalization...
  40. doi request reprint Generation of living color transgenic zebrafish to trace somatostatin-expressing cells and endocrine pancreas organization
    Zhen Li
    Department of Biological Sciences, National University of Singapore, Singapore 119260, Singapore
    Differentiation 77:128-34. 2009
    ..In zebrafish primary islet, beta-cells constitute most of the mass (approximately 50%), followed by delta-cells and alpha-cells (20-25% each), and PP-cells (1-2%); this is also similar to the composition of mammalian islets...
  41. doi request reprint Development of zebrafish swimbladder: The requirement of Hedgehog signaling in specification and organization of the three tissue layers
    Cecilia Lanny Winata
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Biol 331:222-36. 2009
    ..Furthermore, time-specific Hh inhibition by cyclopamine revealed different requirements of Hh signaling in the formation and organization of all three tissue layers of swimbladder...
  42. ncbi request reprint Molecular cloning of zebrafish and medaka vitellogenin genes and comparison of their expression in response to 17beta-estradiol
    Yan Tong
    Department of Biological Sciences, National University of Singapore, Block S2 No 05 17, 14 Science Drive 4, 10 King Ridge Crescent, Singapore 119260, Singapore
    Gene 328:25-36. 2004
    ..The implication of these observations may be that the medaka system is better in monitoring acute treatment while the zebrafish system is better in monitoring chronic exposure...
  43. ncbi request reprint Expression analyses of zebrafish transferrin, ifabp, and elastaseB mRNAs as differentiation markers for the three major endodermal organs: liver, intestine, and exocrine pancreas
    Sudha Puttur Mudumana
    Department of Biological Sciences, National University of Singapore, Singapore
    Dev Dyn 230:165-73. 2004
    ..In contrast to the transferrin and ifabp mRNAs, elastaseB mRNAs were not expressed in the yolk sac or YSL, and these transcripts were detected exclusively in the exocrine pancreas after approximately 56 hpf...
  44. doi request reprint Expression of components of Wnt and Hedgehog pathways in different tissue layers during lung development in Xenopus laevis
    Ao Yin
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    Gene Expr Patterns 10:338-44. 2010
    ....
  45. ncbi request reprint Demonstration of site-directed recombination in transgenic zebrafish using the Cre/loxP system
    Xiufang Pan
    Department of Biological Sciences, National University of Singapore, Singapore
    Transgenic Res 14:217-23. 2005
    ..Our experiments demonstrated that the Cre/loxP system can function efficiently and accurately in the zebrafish system...
  46. doi request reprint Molecular insights into 4-nitrophenol-induced hepatotoxicity in zebrafish: transcriptomic, histological and targeted gene expression analyses
    Siew Hong Lam
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    Biochim Biophys Acta 1830:4778-89. 2013
    ..However, molecular information of 4-NP induced hepatotoxicity is still limited. This study aimed to obtain molecular insights into 4-NP-induced hepatotoxicity using zebrafish as a model...
  47. doi request reprint Delayed and restricted expression of UAS-regulated GFP gene in early transgenic zebrafish embryos by using the GAL4/UAS system
    Huiqing Zhan
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore 117543
    Mar Biotechnol (NY) 12:1-7. 2010
    ..Therefore, the delayed and restricted transgene expression should be taken into consideration when GAL4/UAS system is used to study transgene expression in early developmental stages...
  48. ncbi request reprint Ziwi, the zebrafish homologue of the Drosophila piwi: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults
    Cheong Huat Tan
    Department of Biological Sciences, National University of Singapore, Singapore
    Gene Expr Patterns 2:257-60. 2002
    ..During neurogenesis and organogenesis, its expression was detected in the CNS and fin buds. Starting from 24 hpf and later on, ziwi transcripts were found in the genital ridge...
  49. ncbi request reprint Development of estrogen-responsive transgenic medaka for environmental monitoring of endocrine disrupters
    Zhiqiang Zeng
    Department of Biological Sciences, National University of Singapore, Singapore
    Environ Sci Technol 39:9001-8. 2005
    ..Our experiments indicated the broad application of the transgenic line to monitor a wide range of estrogenic chemicals...
  50. ncbi request reprint Generation of two-color transgenic zebrafish using the green and red fluorescent protein reporter genes gfp and rfp
    Haiyan Wan
    Department of Biological Sciences, National University of Singapore, Singapore 119260
    Mar Biotechnol (NY) 4:146-54. 2002
    ..Our study demonstrates the feasibility of monitoring expression of multiple genes in different tissues in the same transgenic organism...
  51. pmc Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones
    Weiling Zheng
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 8:e53562. 2013
    ..The information obtained in this study provides comprehensive insights into the sexual dimorphism of zebrafish liver transcriptome and will facilitate further development of the zebrafish as a human liver disease model...
  52. ncbi request reprint Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression
    Siew Hong Lam
    Department of Biological Sciences, 14 Science Drive 4, National University of Singapore, Singapore 117543
    Nat Biotechnol 24:73-5. 2006
    ..This approach provides a useful strategy for identifying an expression signature that is strongly associated with a disease phenotype...
  53. pmc Transgenic expression of walleye dermal sarcoma virus rv-cyclin gene in zebrafish and its suppressive effect on liver tumor development after carcinogen treatment
    Huiqing Zhan
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore 117543
    Mar Biotechnol (NY) 12:640-9. 2010
    ..These findings indicate that transgenic fish models are powerful systems for investigating mechanisms of inhibition and regression of liver tumors...
  54. ncbi request reprint Tandem-repeated Zebrafish zp3 genes possess oocyte-specific promoters and are insensitive to estrogen induction
    Xingjun Liu
    Department of Biological Sciences, National University of Singapore, Singapore 119260
    Biol Reprod 74:1016-25. 2006
    ..Thus, the new transgenic line not only provided a convenient living marker for monitoring female gonad development, but also demonstrated that a single zp3 gene promoter is sufficient for oocyte-specific transcription...
  55. doi request reprint Zebrafish spotted-microarray for genome-wide expression profiling experiments. Part I: array printing and hybridization
    Siew Hong Lam
    Department of Biological Sciences, S3 Level 5, 14 Science Drive 4, National University of Singapore, Singapore, 117543
    Methods Mol Biol 546:175-95. 2009
    ..This chapter describes these "wet-lab" procedures. "Dry-lab" procedures are described in the next chapter...
  56. doi request reprint Comparative transcriptome analyses revealed conserved biological and transcription factor target modules between the zebrafish and human tumors
    Choong Yong Ung
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    Zebrafish 6:425-31. 2009
    ....
  57. pmc Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung
    Weiling Zheng
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e24019. 2011
    ..Our comparative analyses also provide molecular evidence of the relatedness of the fish swimbladder and mammalian lung...
  58. doi request reprint Transcriptomic analysis of a transgenic zebrafish hepatocellular carcinoma model reveals a prominent role of immune responses in tumour progression and regression
    Zhen Li
    Department of Biological Sciences, National University of Singapore, Singapore Human genetics 2, Genome Institute of Singapore, Singapore
    Int J Cancer 135:1564-73. 2014
    ....
  59. ncbi request reprint Ziwi, the zebrafish homologue of the Drosophila piwi: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults
    Cheong Huat Tan
    Department of Biological Sciences, National University of Singapore, Singapore
    Mech Dev 119:S221-4. 2002
    ..During neurogenesis and organogenesis, its expression was detected in the CNS and fin buds. Starting from 24 hpf and later on, ziwi transcripts were found in the genital ridge...
  60. doi request reprint Common deregulated gene expression profiles and morphological changes in developing zebrafish larvae exposed to environmental-relevant high to low concentrations of glucocorticoids
    Qiyu Chen
    NUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, Singapore
    Chemosphere 172:429-439. 2017
    ..The common deregulated genes with the associated biological processes and morphological changes can be used for biological inference of glucocorticoid exposure in fish for future studies...
  61. pmc Development of a conditional liver tumor model by mifepristone-inducible Cre recombination to control oncogenic kras V12 expression in transgenic zebrafish
    Anh Tuan Nguyen
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    Sci Rep 6:19559. 2016
    ....
  62. doi request reprint Generation of Tg(cyp1a:gfp) Transgenic Zebrafish for Development of a Convenient and Sensitive In Vivo Assay for Aryl Hydrocarbon Receptor Activity
    Hongyan Xu
    Department of Biological Sciences, National University of Singapore, S3 Level 6, 14 Science Drive 4, Singapore, 117543, Singapore
    Mar Biotechnol (NY) 17:831-40. 2015
    ....
  63. pmc Myc-induced liver tumors in transgenic zebrafish can regress in tp53 null mutation
    Lili Sun
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 10:e0117249. 2015
    ..Furthermore, tumor regression is independent of the function of Tp53...
  64. pmc GFP transgenic medaka (Oryzias latipes) under the inducible cyp1a promoter provide a sensitive and convenient biological indicator for the presence of TCDD and other persistent organic chemicals
    Grace Hwee Boon Ng
    Department of Biological Sciences, NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore
    PLoS ONE 8:e64334. 2013
    ..The GFP inducibility of Tg(cyp1a:gfp) medaka at both fry and adult stages may be useful for development of high-throughput assays as well as online water monitoring system to detect xenobiotic toxicity...
  65. pmc Fluorescent transgenic zebrafish Tg(nkx2.2a:mEGFP) provides a highly sensitive monitoring tool for neurotoxins
    Xiaoyan Zhang
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 8:e55474. 2013
    ..Our study may open a new avenue to use other fluorescent transgenic zebrafish embryos/fry to develop sensitive and specific toxicological tests for different categories of chemicals...
  66. ncbi request reprint Modeling liver cancer using zebrafish: a comparative oncogenomics approach
    Siew Hong Lam
    Department of Biological Sciences, National University of Singapore, Singapore
    Cell Cycle 5:573-7. 2006
    ..In addition, our comparative oncogenomic work provides a general framework for comparing and validating microarray data of zebrafish model with human cancer, thus adding confidence of using the zebrafish to model human cancers...
  67. pmc STORM: a general model to determine the number and adaptive changes of epithelial stem cells in teleost, murine and human intestinal tracts
    Zhengyuan Wang
    Computation and Systems Biology, Singapore MIT Alliance, Singapore, Singapore
    PLoS ONE 5:e14063. 2010
    ..Its general applicability to different species makes it a valuable tool for analysis of intestinal stem cells under various pathological conditions...
  68. pmc Existence of inverted profile in chemically responsive molecular pathways in the zebrafish liver
    Choong Yong Ung
    Department of Biological Sciences, National University of Singapore, Queenstown, Singapore
    PLoS ONE 6:e27819. 2011
    ....
  69. pmc Xmrk, kras and myc transgenic zebrafish liver cancer models share molecular signatures with subsets of human hepatocellular carcinoma
    Weiling Zheng
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    PLoS ONE 9:e91179. 2014
    ....
  70. ncbi request reprint The functional specificity of NeuroD is defined by a single amino acid residue (N11) in the basic domain
    Xukun Wang
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    FEBS Lett 520:139-44. 2002
    ..Our data indicate that the functional specificity of NeuroD to define sensory neurons is mainly due to a single residue (asparagine 11) in its basic domain...
  71. doi request reprint Zebrafish spotted-microarray for genome-wide expression profiling experiments: data acquisition and analysis
    Siew Hong Lam
    Department of Biological Sciences, S3 Level 5, 14 Science Drive 4, National University of Singapore, Singapore, 117543
    Methods Mol Biol 546:197-226. 2009
    ..Principal steps and advisory notes for acquiring, processing, and analyzing data that are commonly associated with various zebrafish expression profiling experiments are described in this chapter...
  72. doi request reprint One step forward: the use of transgenic zebrafish tumor model in drug screens
    Xiaoqian Huang
    Department of Biological Sciences, National University of Singapore, Singapore
    Birth Defects Res C Embryo Today 93:173-81. 2011
    ..It is hopeful that in the near future with automation and analytical tools, drug development processes will be significantly shortened for quick and effective identification of candidate drugs...
  73. doi request reprint Incorporating zebrafish omics into chemical biology and toxicology
    Hendrian Sukardi
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    Zebrafish 7:41-52. 2010
    ..This review is intended to update interested readers with the current omics approaches that have been applied in chemical studies on zebrafish and their potential in enhancing discovery in chemical biology...
  74. ncbi request reprint Proteomic analysis of protein profiles during early development of the zebrafish, Danio rerio
    Tuan Leng Tay
    Department of Biological Sciences, National University of Singapore, Singapore 119260
    Proteomics 6:3176-88. 2006
    ..Some of the proteins with interesting temporal expression profiles during development are further discussed...
  75. doi request reprint The role of autophagy in liver cancer: molecular mechanisms and potential therapeutic targets
    Jianzhou Cui
    Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    Biochim Biophys Acta 1836:15-26. 2013
    ..It is believed that understanding the molecular mechanisms underlying the autophagy modulation and liver cancer development may provoke the translational studies that ultimately lead to new therapeutic strategies for liver cancer...
  76. doi request reprint Generation of transgenic zebrafish with liver-specific expression of EGFP-Lc3: a new in vivo model for investigation of liver autophagy
    Jianzhou Cui
    Saw Swee Hock School of Public Health, National University of Singapore, Singapore
    Biochem Biophys Res Commun 422:268-73. 2012
    ..Thus, the newly established transgenic line will be a useful in vivo model to investigate liver autophagy, and, in particular, the involvement of autophagy in basic biology and diseases in the liver...
  77. doi request reprint Biochemical response of diverse organs in adult Danio rerio (zebrafish) exposed to sub-lethal concentrations of microcystin-LR and microcystin-RR: a balneation study
    Shruti Pavagadhi
    Singapore Delft Water Alliance, National University of Singapore, Singapore 117576, Singapore
    Aquat Toxicol 109:1-10. 2012
    ..This observation suggests that MCs found in trace levels are likely to have deleterious effects on aquatic organisms and can trigger a variety of biochemical responses depending on their specific toxicity...
  78. doi request reprint Zebrafish for drug toxicity screening: bridging the in vitro cell-based models and in vivo mammalian models
    Hendrian Sukardi
    National University of Singapore, Department of Biological Sciences, Singapore
    Expert Opin Drug Metab Toxicol 7:579-89. 2011
    ..A number of studies have been conducted to see if these small fish are up to the task of drug toxicity testing, an important rite of passage along the pharmaceutical pipeline...
  79. doi request reprint Liver tumor models in transgenic zebrafish: an alternative in vivo approach to study hepatocarcinogenes
    Xiaoqian Huang
    Department of Biological Sciences, National University of Singapore, 117543, Singapore
    Future Oncol 8:21-8. 2012
    ....
  80. pmc K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis
    Lihui Liu
    Department of Biological Sciences, Cell Signaling and Developmental Biology Laboratory, The National University of Singapore, Singapore, The Republic of Singapore
    PLoS ONE 3:e2850. 2008
    ....
  81. ncbi request reprint Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary
    Sheng Zeng
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    Gene 294:45-53. 2002
    ..At last, four of the clones were found, by section in situ hybridization, to be expressed specifically in the germ cells of the testis or ovary and thus they are suitable molecular markers for analyses of spermatogenesis and oogenesis...
  82. ncbi request reprint Toxicity of silver nanoparticles in zebrafish models
    P V AshaRani
    Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, 117543, Singapore
    Nanotechnology 19:255102. 2008
    ..Furthermore, the acridine orange staining showed an increased apoptosis in Ag-np treated embryos. These results suggest that silver nanoparticles induce a dose-dependent toxicity in embryos, which hinders normal development...
  83. doi request reprint Comparison of the toxicity of silver, gold and platinum nanoparticles in developing zebrafish embryos
    P V AshaRani
    Department of Chemistry, National University of Singapore, Singapore
    Nanotoxicology 5:43-54. 2011
    ..The toxic effects exhibited by the zebrafish embryos as a consequence of nanoparticle exposure, accompanied by the accumulation of metals inside the body calls for urgent further investigations in this field...
  84. doi request reprint Maize Ac/Ds transposon system leads to highly efficient germline transmission of transgenes in medaka (Oryzias latipes)
    Grace Hwee Boon Ng
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    Biochimie 93:1858-64. 2011
    ..Our data presented here demonstrated the highly efficient transgenesis with the aid of the maize Ac/Ds transposon system...