Nuno F Azevedo

Summary

Affiliation: University of Minho
Country: Portugal

Publications

  1. pmc Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, 4700 057 Braga, Portugal
    Appl Environ Microbiol 70:490-3. 2004
  2. pmc Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples
    C Almeida
    Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 76:4476-85. 2010
  3. pmc Survival of gastric and enterohepatic Helicobacter spp. in water: implications for transmission
    N F Azevedo
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 74:1805-11. 2008
  4. doi Time to "go large" on biofilm research: advantages of an omics approach
    Nuno F Azevedo
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga 4710 057, Portugal
    Biotechnol Lett 31:477-85. 2009
  5. pmc Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment
    N F Azevedo
    Centro de Engenharia Biólogica, Campus de Gualtar, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 73:3423-7. 2007
  6. ncbi A new model for the transmission of Helicobacter pylori: role of environmental reservoirs as gene pools to increase strain diversity
    N F Azevedo
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Crit Rev Microbiol 33:157-69. 2007
  7. pmc Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens
    Laura Cerqueira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    J Clin Microbiol 51:1887-93. 2013
  8. doi Computational approaches to standard-compliant biofilm data for reliable analysis and integration
    Ana Margarida Sousa
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Integr Bioinform 9:203. 2012
  9. doi Comparison between standard culture and peptide nucleic acid 16S rRNA hybridization quantification to study the influence of physico-chemical parameters on Legionella pneumophila survival in drinking water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:343-51. 2009
  10. pmc PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori
    Laura Cerqueira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    BMC Microbiol 11:101. 2011

Collaborators

Detail Information

Publications32

  1. pmc Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, 4700 057 Braga, Portugal
    Appl Environ Microbiol 70:490-3. 2004
    ..Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water...
  2. pmc Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples
    C Almeida
    Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 76:4476-85. 2010
    ....
  3. pmc Survival of gastric and enterohepatic Helicobacter spp. in water: implications for transmission
    N F Azevedo
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 74:1805-11. 2008
    ..felis, H. canadensis, H. pullorum, and H. canis, in water under several conditions and concludes that the roles of water in transmission between hosts are likely to be similar for all these species, whether enterohepatic or not...
  4. doi Time to "go large" on biofilm research: advantages of an omics approach
    Nuno F Azevedo
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga 4710 057, Portugal
    Biotechnol Lett 31:477-85. 2009
    ..Whether such device and analytical methods are already available, particularly for complex heterotrophic biofilms is, however, very debatable...
  5. pmc Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment
    N F Azevedo
    Centro de Engenharia Biólogica, Campus de Gualtar, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 73:3423-7. 2007
    ..This strongly supports the view that transformation into the coccoid form is an active, biologically led process, switched on by the bacterium as a protection mechanism...
  6. ncbi A new model for the transmission of Helicobacter pylori: role of environmental reservoirs as gene pools to increase strain diversity
    N F Azevedo
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Crit Rev Microbiol 33:157-69. 2007
    ..pylori...
  7. pmc Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens
    Laura Cerqueira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    J Clin Microbiol 51:1887-93. 2013
    ..pylori strains can subsist in very close proximity in the stomach...
  8. doi Computational approaches to standard-compliant biofilm data for reliable analysis and integration
    Ana Margarida Sousa
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Integr Bioinform 9:203. 2012
    ..org) - and a public repository on colony morphology signatures - MorphoCol (http://stardust.deb.uminho.pt/morphocol)...
  9. doi Comparison between standard culture and peptide nucleic acid 16S rRNA hybridization quantification to study the influence of physico-chemical parameters on Legionella pneumophila survival in drinking water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:343-51. 2009
    ..pneumophila. This raises public health concerns since culture methods are still considered to be the gold standard for assessing the presence of this opportunistic pathogen in water...
  10. pmc PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori
    Laura Cerqueira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    BMC Microbiol 11:101. 2011
    ..In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent in situ hybridization (FISH) using a set of peptide nucleic acid probes (PNA), is proposed...
  11. doi Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress
    M S Gião
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:335-41. 2009
    ..pneumophila from re-entering an infectious state by maintaining residual disinfectant levels through the entire DWDS network so that the resuscitation of cells via contact with amoebae is prevented...
  12. doi Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Microb Ecol 58:56-62. 2009
    ..pneumophila cells in the environment...
  13. pmc Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Appl Environ Microbiol 74:5898-904. 2008
    ....
  14. pmc Effect of chlorine on incorporation of Helicobacter pylori into drinking water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 76:1669-73. 2010
    ..Coupled with data obtained using viability stains in pure culture, this result suggests that H. pylori can survive chlorination but remain undetectable by culture methods, which can be effectively replaced by PNA hybridization...
  15. pmc Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens
    N Guimaraes
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    J Clin Microbiol 45:3089-94. 2007
    ..has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens...
  16. ncbi Use of fluorescent in situ hybridisation for the visualisation of Helicobacter pylori in real drinking water biofilms
    S M Braganra
    Centro de Engenharia Biólogica, Universidade do Minho, 4700 057 Braga, Portugal
    Water Sci Technol 55:387-93. 2007
    ..The method requires, however, highly trained personnel for an accurate detection of the pathogen and will need simplification before being routinely used in standard water analysis laboratories...
  17. ncbi Adhesion of water stressed Helicobacter pylori to abiotic surfaces
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, Braga, Portugal
    J Appl Microbiol 101:718-24. 2006
    ....
  18. pmc Shear stress, temperature, and inoculation concentration influence the adhesion of water-stressed Helicobacter pylori to stainless steel 304 and polypropylene
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 72:2936-41. 2006
    ..It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms...
  19. pmc Polymicrobial Ventilator-Associated Pneumonia: Fighting In Vitro Candida albicans-Pseudomonas aeruginosa Biofilms with Antifungal-Antibacterial Combination Therapy
    Maria E Rodrigues
    Centre of Biological Engineering, LIBRO Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, Braga, Portugal
    PLoS ONE 12:e0170433. 2017
    ....
  20. pmc DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH)
    Laura Cerqueira
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
    Int J Mol Sci 9:1944-60. 2008
    ....
  21. doi An harmonised vocabulary for communicating and interchanging biofilms experimental results
    Ana Margarida Sousa
    CEB Centre of Biological Engineering, LIBRO Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Integr Bioinform 11:249. 2014
    ..Information branches will be added, extended or refactored according to user feedback and group discussions. ..
  22. pmc Discriminating multi-species populations in biofilms with peptide nucleic acid fluorescence in situ hybridization (PNA FISH)
    Carina Almeida
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualta, Braga, Portugal
    PLoS ONE 6:e14786. 2011
    ..However, the lack of reliable techniques for the discrimination of each population has meant that studies focusing on multi-species biofilms are scarce and typically generate qualitative rather than quantitative data...
  23. doi Discriminating typical and atypical cystic fibrosis-related bacteria by multiplex PNA-FISH
    Susana P Lopes
    Centre of Biological Engineering, LIBRO Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Bioeng . 2016
    ..Biotechnol. Bioeng. 2016;9999: 1-13. © 2016 Wiley Periodicals, Inc...
  24. doi The cystic fibrosis microbiome in an ecological perspective and its impact in antibiotic therapy
    Andreia P Magalhães
    CEB Centre of Biological Engineering, LIBRO Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Appl Microbiol Biotechnol 100:1163-81. 2016
    ..Consequently, a new approach where therapy is personalized to patients by taking into account their individual CF microbiome is suggested. ..
  25. doi Microbiome in cystic fibrosis: Shaping polymicrobial interactions for advances in antibiotic therapy
    Susana P Lopes
    IBB CEB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal and
    Crit Rev Microbiol 41:353-65. 2015
    ..If this is the case, management of CF will only be successful upon suitable and exhaustive modulation of such mixed ecological processes, which will also be useful to predict the effects of new therapeutic interventions. ..
  26. pmc Emergent bacteria in cystic fibrosis: in vitro biofilm formation and resilience under variable oxygen conditions
    Susana P Lopes
    Institute for Biotechnology and Bioengineering IBB, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biomed Res Int 2014:678301. 2014
    ..pigrum on CF lung infections, demonstrating capacity to adapt to biofilm mode of life under restricted-oxygen atmospheres resembling CF airways, which may ultimately endanger the efficacy of currently used antibiotic regimens. ..
  27. pmc BiofOmics: a Web platform for the systematic and standardized collection of high-throughput biofilm data
    Anália Lourenço
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    PLoS ONE 7:e39960. 2012
    ..However, biofilm data is widespread across ad hoc, non-standardized individual files and, thus, data interchange among researchers, or any attempt of cross-laboratory experimentation or analysis, is hardly possible or even attempted...
  28. ncbi Critical review on biofilm methods
    Joana Azeredo
    a CEB Centre of Biological Engineering, LIBRO, Laboratórios de Biofilmes Rosário Oliveira, University of Minho Campus de Gualtar, Braga, Portugal
    Crit Rev Microbiol . 2016
    ..Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms...
  29. pmc Minimum information about a biofilm experiment (MIABiE): standards for reporting experiments and data on sessile microbial communities living at interfaces
    Anália Lourenço
    Departamento de Informatica, Universidade de Vigo, ESEI Escuela Superior de Ingeniería Informática, Ourense, Spain IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    Pathog Dis 70:250-6. 2014
    ....
  30. pmc Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
    C Almeida
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 75:2925-30. 2009
    ..FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants...
  31. pmc Detection of Escherichia coli in biofilms from pipe samples and coupons in drinking water distribution networks
    T Juhna
    Riga Technical University, Department of Water Engineering and Technology, 16 20 Azenes Street, Riga LV 1048, Latvia
    Appl Environ Microbiol 73:7456-64. 2007
    ....
  32. ncbi Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms
    N F Azevedo
    Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK
    Water Sci Technol 47:155-60. 2003
    ..PNA probes provided an easy and quick way of performing fluorescence in situ hybridisation assays in heterogeneous biofilms...