Krystyna Michalak

Summary

Country: Poland

Publications

  1. ncbi request reprint Lipid membrane perturbation caused by some isoflavones and phenothiazines, and the activity of these compounds as inhibitors of multidrug resistance
    Krystyna Michalak
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    Cell Mol Biol Lett 7:293. 2002
  2. ncbi request reprint Polyphenols counteract tumor cell chemoresistance conferred by multidrug resistance proteins
    Krystyna Michalak
    Wrocław Medical University, Department of Biophysics, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Anticancer Agents Med Chem 12:880-90. 2012
  3. ncbi request reprint [Role of cell membrane lipids in multidrug resistance and its modulation]
    Krystyna Michalak
    Katedra i Zakład Biofizyki, Akademia Medyczna we Wrocławiu, ul Chałubińskiego 10, 50 368 Wrocław
    Postepy Biochem 48:208-19. 2002
  4. ncbi request reprint Influence of silybin on biophysical properties of phospholipid bilayers
    Olga Wesołowska
    Department of Biophysics, Wroclaw Medical University, ul Chalubinskiego 10, 50 368 Wroclaw, Poland
    Acta Pharmacol Sin 28:296-306. 2007
  5. doi request reprint Multidrug resistance reversal and apoptosis induction in human colon cancer cells by some flavonoids present in citrus plants
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    J Nat Prod 75:1896-902. 2012
  6. doi request reprint 8-Prenylnaringenin is an inhibitor of multidrug resistance-associated transporters, P-glycoprotein and MRP1
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Eur J Pharmacol 644:32-40. 2010
  7. ncbi request reprint New phenothiazine-type multidrug resistance modifiers: anti-MDR activity versus membrane perturbing potency
    Andrzej B Hendrich
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368, Wrocław, Poland
    Biochem Biophys Res Commun 304:260-5. 2003
  8. ncbi request reprint Differential effect of phenothiazines on MRP1 and P-glycoprotein activity
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    In Vivo 23:943-7. 2009
  9. ncbi request reprint Presence of anionic phospholipids rules the membrane localization of phenothiazine type multidrug resistance modulator
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biophys Chem 109:399-412. 2004
  10. doi request reprint Perturbation of the lipid phase of a membrane is not involved in the modulation of MRP1 transport activity by flavonoids
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Cell Mol Biol Lett 14:199-221. 2009

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Lipid membrane perturbation caused by some isoflavones and phenothiazines, and the activity of these compounds as inhibitors of multidrug resistance
    Krystyna Michalak
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    Cell Mol Biol Lett 7:293. 2002
  2. ncbi request reprint Polyphenols counteract tumor cell chemoresistance conferred by multidrug resistance proteins
    Krystyna Michalak
    Wrocław Medical University, Department of Biophysics, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Anticancer Agents Med Chem 12:880-90. 2012
    ..The mechanism of flavonoids interactions with the multidrug transporters and the structure-activity relationship are also discussed...
  3. ncbi request reprint [Role of cell membrane lipids in multidrug resistance and its modulation]
    Krystyna Michalak
    Katedra i Zakład Biofizyki, Akademia Medyczna we Wrocławiu, ul Chałubińskiego 10, 50 368 Wrocław
    Postepy Biochem 48:208-19. 2002
  4. ncbi request reprint Influence of silybin on biophysical properties of phospholipid bilayers
    Olga Wesołowska
    Department of Biophysics, Wroclaw Medical University, ul Chalubinskiego 10, 50 368 Wroclaw, Poland
    Acta Pharmacol Sin 28:296-306. 2007
    ..Although membranes are postulated to be one of the cellular targets for silybin, little is known about its interaction with phospholipid bilayers...
  5. doi request reprint Multidrug resistance reversal and apoptosis induction in human colon cancer cells by some flavonoids present in citrus plants
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    J Nat Prod 75:1896-902. 2012
    ..Its apoptosis-inducing activity was also higher in LoVo/Dx than in LoVo cells. It was concluded that the activity of 3 against multidrug-resistant cancer cells may be enhanced by its apoptosis-inducing activity. ..
  6. doi request reprint 8-Prenylnaringenin is an inhibitor of multidrug resistance-associated transporters, P-glycoprotein and MRP1
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Eur J Pharmacol 644:32-40. 2010
    ..To our best knowledge, this is the first report of 8-prenylnaringenin interaction with clinically important ABC transporters...
  7. ncbi request reprint New phenothiazine-type multidrug resistance modifiers: anti-MDR activity versus membrane perturbing potency
    Andrzej B Hendrich
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368, Wrocław, Poland
    Biochem Biophys Res Commun 304:260-5. 2003
    ..It is concluded that the biological and biophysical activity of phenothiazine derivatives depends more on the type of ring substitution than on the nature of the side chain group...
  8. ncbi request reprint Differential effect of phenothiazines on MRP1 and P-glycoprotein activity
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    In Vivo 23:943-7. 2009
    ....
  9. ncbi request reprint Presence of anionic phospholipids rules the membrane localization of phenothiazine type multidrug resistance modulator
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biophys Chem 109:399-412. 2004
    ..Behavior of equimolar PC:PS mixtures is similar to pure PS bilayers, while 2:1 or 1:2 (mole:mole) PC:PS mixtures resemble pure PC ones...
  10. doi request reprint Perturbation of the lipid phase of a membrane is not involved in the modulation of MRP1 transport activity by flavonoids
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Cell Mol Biol Lett 14:199-221. 2009
    ..We concluded that mechanisms other than perturbation of the lipid phase of membranes were responsible for inhibition of MRP1 by the flavonoids...
  11. ncbi request reprint The alterations of lipid bilayer fluidity induced by newly synthesized phenothiazine derivative
    Andrzej B Hendrich
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368, Wrocław, Poland
    Biophys Chem 98:275-85. 2002
    ..This structure is less co-operative than an unperturbed bilayer, but locally the mobility of lipid molecules is decreased...
  12. ncbi request reprint A study on the perturbation of model lipid membranes by phenoxazines
    Andrzej B Hendrich
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    Bioorg Med Chem 14:5948-54. 2006
    ..These studies showed also that phenoxazine molecules are located close to the polar/apolar interface of bilayer. The results allow to conclude that phenoxazines rather weakly interact with lipid bilayers...
  13. doi request reprint Direct visualization of phase separation induced by phenothiazine-type antipsychotic drugs in model lipid membranes
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    Mol Membr Biol 28:103-14. 2011
    ..To the best of our knowledge, this is the first report demonstrating the influence of drugs on domain morphology directly visualized in giant unilamellar vesicles...
  14. ncbi request reprint Euphorbia Species-derived Diterpenes and Coumarins as Multidrug Resistance Modulators in Human Colon Carcinoma Cells
    Jerzy Wisniewski
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland Department of Biochemistry, Wrocław Medical University, Wrocław, Poland
    Anticancer Res 36:2259-64. 2016
    ....
  15. doi request reprint Substrates and modulators of the multidrug transporter Cdr1p of Candida albicans in antifungal extracts of medicinal plants
    Marcin Kolaczkowski
    Department of Biophysics, Wroclaw Medical University, Wroclaw, Poland
    Mycoses 53:305-10. 2010
    ..The presence of a non-toxic Cdr1p-mediated ketoconazole resistance modulator accompanying growth-inhibitory Cdr1p substrates in this extract was revealed by further fractionation experiments...
  16. ncbi request reprint Metabolic O-demethylation does not alter the influence of isoflavones on the biophysical properties of membranes and MRP1-like protein transport activity
    Barbara Łania-Pietrzak
    Department of Biophysics, Wrocław Medical University, Poland
    Arch Biochem Biophys 433:428-34. 2005
    ..Inhibition of MRP1-like protein transport activity by methylated and demethylated isoflavones was very similar. Their influence on lipid thermotropic properties and fluidity of lipid bilayer was not also significantly different...
  17. ncbi request reprint Modulation of MRP1 protein transport by plant, and synthetically modified flavonoids
    Barbara Łania-Pietrzak
    Department of Biophysics, Wrocław Medical University, Chałubińskiego 10, 50 368 Wrocław, Poland
    Life Sci 77:1879-91. 2005
    ..In case of naturally occurring isoflavones the replacement of hydrogen at position 4' by hydrophobic ring structure seems also to be favourable for inhibition potency...
  18. doi request reprint Genistein derivatives decrease liposome membrane integrity--calcein release and molecular modeling study
    Kamila Sroda
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biophys Chem 138:78-82. 2008
    ..This conclusion may have some implications for QSAR studies in which mostly the dipole moments of entire molecules are considered...
  19. doi request reprint Interaction of the chemopreventive agent resveratrol and its metabolite, piceatannol, with model membranes
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biochim Biophys Acta 1788:1851-60. 2009
    ....
  20. ncbi request reprint Phase separation is induced by phenothiazine derivatives in phospholipid/sphingomyelin/cholesterol mixtures containing low levels of cholesterol and sphingomyelin
    Andrzej B Hendrich
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biophys Chem 130:32-40. 2007
    ..Among three phenothiazine derivatives studied, only trifluoperazine was able to induce a permanent phase separation in phosphatidylcholine/cholesterol/sphingomyelin systems...
  21. ncbi request reprint Phenothiazine maleates stimulate MRP1 transport activity in human erythrocytes
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biochim Biophys Acta 1720:52-8. 2005
    ..We conclude that phenothiazine maleates probably exert their stimulatory effect on MRP1 by direct interaction with the protein at the site different from the substrate binding site...
  22. ncbi request reprint Benzo[a]phenoxazines: a new group of potent P-glycoprotein inhibitors
    Olga Wesolowska
    Department of Biophysics, Wroclaw Medical University, ul Chalubinskiego 10, 50 368 Wroclaw, Poland
    In Vivo 20:109-13. 2006
    ..e. to be new effective MDR modulators. The results allowed us to draw preliminary conclusions about the structural features of benzo[a]phenoxazines which are important for MDR modulation...
  23. ncbi request reprint Stilbenes as multidrug resistance modulators and apoptosis inducers in human adenocarcinoma cells
    Olga Wesolowska
    Department of Biophysics, Wrocław Medical University, 50 368 Wrocław, Poland
    Anticancer Res 30:4587-93. 2010
    ..The identification of compounds that overcome the apoptosis deficiency that frequently accompanies MDR is also of great therapeutic importance...
  24. pmc New high-throughput screening assay to reveal similarities and differences in inhibitory sensitivities of multidrug ATP-binding cassette transporters
    Marcin Kolaczkowski
    Department of Biophysics, Wroclaw Medical University, Wroclaw, Poland
    Antimicrob Agents Chemother 53:1516-27. 2009
    ..The most active derivative, M961, potentiated the activity of KTC against an azole-resistant CDR1-overexpressing C. albicans isolate...
  25. ncbi request reprint A new phenothiazine derivative alters the fluidity and thermotropic behaviour of phosphatidylcholine model membranes
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, Wrocław, 50 368 Poland
    Cell Mol Biol Lett 7:303. 2002
  26. doi request reprint Fluorescence and ESR spectroscopy studies on the interaction of isoflavone genistein with biological and model membranes
    Michał Kuzdzał
    Department of Biophysics, Wrocław Medical University, Poland
    Chem Phys Lipids 164:283-91. 2011
    ..According to our best knowledge this is the first study on modification of domain structure of membranes by genistein...
  27. ncbi request reprint Determination of lipid bilayer/water partition coefficient of new phenothiazines using the second derivative of absorption spectra method
    Andrzej Poła
    Department of Biophysics, Wrocław Medical University, ul Chalubinskiego 10, 50 368 Wroclaw, Poland
    Eur J Pharm Sci 21:421-7. 2004
    ..7-fold when length of the alkyl phenothiazine chain was enhanced by addition of the each next one (-CH(2)) group. Substitution of -H atom by -CF(3) group at position 2 of phenothiazine ring results in 3.5-fold increase in K(p) values...
  28. ncbi request reprint The influence of phenothiazine derivatives on intracellular accumulation of cationic cyanine dye DiOC6(3) in LoVo-DX cells
    Andrzej Pola
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 9, 50 368 Wrocław, Poland
    Anticancer Res 33:857-63. 2013
    ..This study aimed to evaluate the influence of phenothiazine derivatives (PDs) on the intracellular accumulation of cyanine dye DiOC6(3) in doxorubicin-resistant LoVo-DX cell line, with overexpression of P-glycoprotein...
  29. ncbi request reprint Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening
    Olga Wesolowska
    Department of Biophysics, Wroclaw Medical University, 50 368 Wroclaw, Poland
    Anticancer Res 25:383-9. 2005
    ..We conclude that the MES-SA and MES-SA/Dx5 cell line pair constitute a good model for MDR modulators study...
  30. ncbi request reprint Inhibition of P-glycoprotein transport function by N-acylphenothiazines
    Olga Wesolowska
    Department of Biophysics, Wroclaw Medical University, ul Chalubinskiego 10, 50 368 Wroclaw, Poland
    Anticancer Res 22:2863-7. 2002
    ..As a result of the present study a new group of mdr reversal agents was identified...
  31. doi request reprint A conserved interdomain communication pathway of pseudosymmetrically distributed residues affects substrate specificity of the fungal multidrug transporter Cdr1p
    Marcin Kolaczkowski
    Department of Biophysics, Wroclaw Medical University, PL 50 368 Wroclaw, Poland
    Biochim Biophys Acta 1828:479-90. 2013
    ..Our results shed new light on the communication between the pseudosymmetrically arranged domains in a fungal PDR ABC transporter and reveal its profound influence on substrate specificity...
  32. ncbi request reprint The voltage- and time-dependent blocking effect of trifluoperazine on T lymphocyte Kv1.3 channels
    Andrzej Teisseyre
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Biochem Pharmacol 65:551-61. 2003
    ..3 channels only when applied extracellularly. The inhibitory effect of all the compounds was reversible. The possible physiological significance of the current inhibition is discussed...
  33. ncbi request reprint Inhibition of MRP1 transport activity by phenolic and terpenic compounds isolated from Euphorbia species
    Olga Wesolowska
    Department of Biophysics, Wroclaw Medical University, ul Chalubinskiego 10, 50 368 Wroclaw, Poland
    Anticancer Res 27:4127-33. 2007
    ..A search for inhibitors of multidrug resistance (MDR)-associated protein 1 (MRP1) was performed among the compounds isolated from Euphorbia species, plants traditionally used in folk medicine...
  34. ncbi request reprint Differential interaction of Sophora isoflavonoids with lipid bilayers
    Andrzej Boguslaw Hendrich
    Department of Biophysics, Wroclaw Medical University, ul Chalubinskiego 10, 50 368, Wroclaw, Poland
    Eur J Pharm Sci 16:201-8. 2002
    ..Comparing our results with biological tests it seems that interactions with the hydrophobic core of membranes are responsible for the activity of the studied isoflavones...
  35. ncbi request reprint Spectrophotometric determination of the phosphatidylcholine liposomes/water partition coefficients and the protonation equilibrium constants of phenothiazine derivatives
    Andrzej Poła
    Department of Biophysics, Wrocaw Medical University, 50 368 Wrocław, Poland
    Cell Mol Biol Lett 7:295. 2002
  36. ncbi request reprint Giant unilamellar vesicles - a perfect tool to visualize phase separation and lipid rafts in model systems
    Olga Wesołowska
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    Acta Biochim Pol 56:33-9. 2009
    ..The experience of the authors in setting up the GUV-forming equipment and production of GUVs is also presented...
  37. ncbi request reprint Inhibition of the activity of human lymphocyte Kv1.3 potassium channels by resveratrol
    Andrzej Teisseyre
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368, Wrocław, Poland
    J Membr Biol 214:123-9. 2006
    ..The possible mechanism of the inhibitory effect and its significance for biological activity of resveratrol are discussed...
  38. ncbi request reprint The influence of zinc on the modulatory effect of sphingosylphosphorylcholine on Kv1.3 channels in human T lymphocytes
    Andrzej Teisseyre
    Department of Biophysics, Wrocław Medical University, ul Chałubińskiego 10, 50 368 Wrocław, Poland
    Eur Biophys J 33:543-8. 2004
    ..Possible mechanisms underlying the observed phenomena and their possible physiological significance are discussed...
  39. pmc Inhibition of Kv1.3 Channels in Human Jurkat T Cells by Xanthohumol and Isoxanthohumol
    Justyna Gąsiorowska
    Department of Biophysics, Wroclaw Medical University, ul Chałubińskiego 10, 50 368, Wrocław, Poland
    J Membr Biol 248:705-11. 2015
    ..The inhibition of Kv1.3 channels might be involved in antiproliferative and proapoptotic effects of the compounds observed in cancer cell lines expressing these channels. ..
  40. ncbi request reprint Effect of new oxicam derivatives on efflux pumps overexpressed in resistant a human colorectal adenocarcinoma cell line
    Kamila Sroda-Pomianek
    Department of Biophysics, Wroclaw Medical University, Wroclaw, Poland
    Anticancer Res 35:2835-40. 2015
    ..We studied antitumor activity of newly synthesized oxicam derivatives (PR17 and PR18) against doxorubicin-sensitive and resistant human colorectal adenocarcinoma cells (LoVo and LoVo/Dx)...
  41. ncbi request reprint Thioridazine induces erythrocyte stomatocytosis due to interactions with negatively charged lipids
    Andrzej B Hendrich
    Department of Biophysics, Wrocław Medical University, Chałubińskiego 10, 50 368 Wrocław, Poland
    Cell Mol Biol Lett 7:1081-6. 2002
    ..We found that erythrocyte stomatocytosis induced by the drug might be related to preferential interaction of thioridazine with charged lipids...
  42. ncbi request reprint Phenothiazines as potent modulators of yeast multidrug resistance
    Marcin Kolaczkowski
    Department of Biophysics, Wrocław Medical University, Chalubinskiego 10, PL 50 368 Wrocław, Poland
    Int J Antimicrob Agents 22:279-83. 2003
    ..They were synergistic with the antifungal ketoconazole at micromolar concentrations. The most active phenothiazines contained the amino group at the end of the alkylene chain substituent...
  43. doi request reprint Voltage-Gated Potassium Channels Kv1.3--Potentially New Molecular Target in Cancer Diagnostics and Therapy
    Andrzej Teisseyre
    Department of Biophysics, Wroclaw Medical University, Poland
    Adv Clin Exp Med 24:517-24. 2015
    ..A possible application of some compounds from these groups to support therapy of cancer diseases, such as breast, colon and lymph node cancer, and melanoma or chronic lymphocytic leukemia (B-CLL), is announced. ..
  44. ncbi request reprint The influence of 8-prenylnaringenin on the activity of voltage-gated Kv1.3 potassium channels in human Jurkat T cells
    Justyna Gąsiorowska
    Department of Biophysics, Wrocław Medical University, Wrocław, Poland
    Cell Mol Biol Lett 17:559-70. 2012
    ..The inhibition of the Kv1.3 channels might be involved in the antiproliferative and pro-apoptotic effects of 8-prenylnaringenin that have been observed in cancer cell lines expressing these channels...
  45. ncbi request reprint Biochanin A similarly influences the fluidity of liposomes formed from charged and zwitterionic lipids
    Andrzej B Hendrich
    Department of Biophysics, Wrocław University of Medicine, Chałubińskiego 10, 50 368 Wrocław, Poland
    Cell Mol Biol Lett 7:284. 2002
  46. ncbi request reprint The influence of membrane lipid metabolites on lymphocyte potassium channel activity
    Andrzej Teisseyre
    Department of Biophysics, Wrocław Medical University, Chałubińskiego 10, 50 368 Wrocław, Poland
    Cell Mol Biol Lett 7:1095-109. 2002
    ..It is concluded that the effect of SPC is specific and may be due to the presence of a choline residue in SPC molecules. The possible mechanism and the physiological significance of this modulatory effect on Kv1.3 channels are discussed...
  47. ncbi request reprint Flavonoids as inhibitors of MRP1-like efflux activity in human erythrocytes. A structure-activity relationship study
    Małgorzata Bobrowska-Hägerstrand
    Department of Biology, Abo Akademi University, FIN 20520 Abo Turku, Finland
    Oncol Res 13:463-9. 2003
    ..It is suggested that BCPCF efflux is mainly due to MRP1 activity. Our results indicate that flavonoid molecular structure provides a promising base for development of potent MRP1 inhibitors...
  48. ncbi request reprint Antimicrobial activity of N-acylphenothiazines and their influence on lipid model membranes and erythrocyte membranes
    Noboru Motohashi
    Meiji Pharmaceutical University, Kiyose, Tokyo, Japan
    Arzneimittelforschung 53:590-9. 2003
    ..Above results show that phenothiazine maleates were generally more effective than other phenothiazines used in this study...