William A Laing
Affiliation: The Horticulture and Food Research Institute of New Zealand
Country: New Zealand
- The missing step of the L-galactose pathway of ascorbate biosynthesis in plants, an L-galactose guanyltransferase, increases leaf ascorbate contentWilliam A Laing
Horticultural and Food Research Institute of New Zealand, PB 92160, Auckland 1142, New Zealand
Proc Natl Acad Sci U S A 104:9534-9. 2007..Transient expression of this A. chinensis gene in tobacco leaves resulted in a >3-fold increase in leaf ascorbate as well as a 50-fold increase in GDP-L-galactose-D-mannose-1-phosphate guanyltransferase activity...
- Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesisSean M Bulley
Plant and Food Research, PB 92169, Auckland, New Zealand
J Exp Bot 60:765-78. 2009..These studies show the importance of GDP-L-galactose guanyltransferase as a rate-limiting step to AsA, and demonstrate how AsA can be significantly increased in plants...
- A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesisWilliam A Laing
Gene Technologies Sector, Horticultural and Food Research Institute of New Zealand, Ltd, PB 92169, Auckland, New Zealand
Proc Natl Acad Sci U S A 101:16976-81. 2004..The data are discussed in terms of the pathway to ascorbate biosynthesis in plants...
- Enhancing ascorbate in fruits and tubers through over-expression of the L-galactose pathway gene GDP-L-galactose phosphorylaseSean Bulley
The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand
Plant Biotechnol J 10:390-7. 2012..In both strawberry and tomato, an increase in polyphenolic content was associated with increased ascorbate. These results show that GGP can be used to raise significantly ascorbate concentration in commercially significant edible crops...
- Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripeningRoss N Crowhurst
The Horticultural and Food Research Institute of New Zealand, PB 92169, Auckland, New Zealand
BMC Genomics 9:351. 2008..To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags (ESTs)...
- Dietary flavonoids from modified apple reduce inflammation markers and modulate gut microbiota in miceRichard V Espley
The New Zealand Institute for Plant and Food Research Limited PFR, Auckland, New Zealand
J Nutr 144:146-54. 2014..01). In summary, high-flavonoid apple was associated with decreases in some inflammation markers and changes in gut microbiota when fed to healthy mice...
- Unusual immuno-modulatory triterpene-caffeates in the skins of russeted varieties of apples and pearsChristelle M Andre
The New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Private Bag 92 169, Auckland 1142, New Zealand
J Agric Food Chem 61:2773-9. 2013....
- Effects of kiwifruit extracts on colonic gene and protein expression levels in IL-10 gene-deficient miceShelley J Edmunds
Food Innovation, Plant and Food Research Limited, Auckland, New Zealand
Br J Nutr 108:113-29. 2012....
- Ethylene-regulated (methylsulfanyl)alkanoate ester biosynthesis is likely to be modulated by precursor availability in Actinidia chinensis genotypesCatrin S Günther
The New Zealand Institute for Plant and Food Research Ltd, Private Bag 92169, Auckland 1142, New Zealand
J Plant Physiol 168:629-38. 2011..chinensis kiwifruit and a close connection between (methylsulfanyl)alkanoate ester formation and ethylene synthesis in plants is proposed. Finally, a possible biosynthetic pathway is presented...
- MYB transcription factors that colour our fruitAndrew C Allan
HortResearch, Mt Albert Research Centre, Private Bag 92169, Auckland, New Zealand
Trends Plant Sci 13:99-102. 2008..These findings lead the way to new approaches in the breeding and biotechnological development of fruit with new colour patterns...
- Identification and characterisation of proteinase inhibitors and their genes from seeds of apple (Malus domestica)Stuart N Ryan
Horticulture and Food Research Institute of New Zealand, Private Bag 92169, Auckland, New Zealand
J Biochem 134:31-42. 2003..The relationship of these inhibitors with those of other species is discussed...
- Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plantsRoger P Hellens
HortResearch, Mt Albert Research Centre, Private Bag 92169, Auckland, New Zealand
Plant Methods 1:13. 2005..We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformation such as transformation selection genes...
- An upstream open reading frame is essential for feedback regulation of ascorbate biosynthesis in ArabidopsisWilliam A Laing
The New Zealand Institute for Plant and Food Research, Auckland 1142, New Zealand
Plant Cell 27:772-86. 2015..This posttranslational regulation of ascorbate is likely an ancient mechanism of control as the uORF is conserved in GGP genes from mosses to angiosperms. ..
- Quantification of folate in fruits and vegetables: A fluorescence-based homogeneous assayHarry Martin
New Zealand Institute for Plant and Food Research, New Zealand
Anal Biochem 402:137-45. 2010..These data are similar to published values. However, the assay will not detect 5-formyltetrahydrofolate which is a significant constituent of the total folate in lettuce, spinach, carrot, and peppers...
- The squash aspartic proteinase inhibitor SQAPI is widely present in the cucurbitales, comprises a small multigene family, and is a member of the phytocystatin familyJohn T Christeller
Horticulture and Food Research Institute, Private Bag 11030, Palmerston North, New Zealand
J Mol Evol 63:747-57. 2006..This appears to be the first instance of a cystatin being recruited as a proteinase inhibitor of another proteinase family...
- Purification and characterization of phytocystatins from kiwifruit cortex and seedsMaysoon Rassam
Gene Technologies, The Horticultural and Food Research Institute of New Zealand, PB 92169, Auckland, New Zealand
Phytochemistry 65:19-30. 2004..This is the first report of phytocystatins from the Ericales...
- Anti-inflammatory procyanidins and triterpenes in 109 apple varietiesChristelle M Andre
New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Private Bag 92 169, Auckland 1142, New Zealand
J Agric Food Chem 60:10546-54. 2012..This study provides new insights into how apple genetic diversity could be used to alleviate inflammation...
- Kiwifruit extracts inhibit cytokine production by lipopolysaccharide-activated macrophages, and intestinal epithelial cells isolated from IL10 gene deficient miceShelley J Edmunds
Food Innovation, The New Zealand Institute for Plant and Food Research Ltd, Auckland 1142, New Zealand
Cell Immunol 270:70-9. 2011..These results suggest that kiwifruit extracts have significant anti-inflammatory activity relevant to IBD. We suggest that the Il10(-/-) mouse is a suitable model for further study of these compounds...
- Analyses of expressed sequence tags from appleRichard D Newcomb
Horticultural and Food Research Institute of New Zealand Limited, Mt Albert Research Centre, Auckland, New Zealand
Plant Physiol 141:147-66. 2006..This resource paves the way for a concerted functional genomics effort in this important temperate fruit crop...
- Analysis of the interaction between the aspartic peptidase inhibitor SQAPI and aspartic peptidases using surface plasmon resonancePeter C Farley
Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
J Mol Recognit 15:135-44. 2002..albicans rSap1 and Sap2 isoenzymes (inhibition constants 25 and 400 nM, respectively) was, in each case, characterized by a larger dissociation rate constant...
- Physiological and biochemical leaf and tree responses to crop load in appleJens N Wünsche
HortResearch, Hawke s Bay Research Centre, Private Bag 1401, Havelock North, New Zealand
Tree Physiol 25:1253-63. 2005..Increases in xanthophyll cycle carotenoids mediated non-radiative thermal energy dissipation in non-cropping trees, providing increased capacity for photoprotection but reducing photochemical efficiency...
- The expression of a mammalian proteinase inhibitor, bovine spleen trypsin inhibitor in tobacco and its effects on Helicoverpa armigera larvaeJohn T Christeller
The Horticulture and Food Research Institute of New Zealand, Palmerston North Research Centre
Transgenic Res 11:161-73. 2002..Activities of leucine aminopeptidase and elastase-like chymotrypsin remained unaltered. The use of SI as an insect resistance factor is discussed...