Javier Milara

Summary

Publications

  1. pmc Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients
    Javier Milara
    Department of Pharmacology, Faculty of Medicine, Jaume I University, Castellon, Spain
    Respir Res 17:145. 2016
  2. doi request reprint MUC4 impairs the anti-inflammatory effects of corticosteroids in patients with chronic rhinosinusitis with nasal polyps
    Javier Milara
    Department of Pharmacy, University Hospital Consortium, Valencia, Spain Department of Pharmacology, Faculty of Medicine, Jaume I University, Castellon, Spain Department of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain CIBERES, Health Institute Carlos III, Valencia, Spain Electronic address
    J Allergy Clin Immunol . 2016
  3. doi request reprint Vascular effects of sildenafil in patients with pulmonary fibrosis and pulmonary hypertension: an ex vivo/in vitro study
    Javier Milara
    Dept of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain Clinical Research Unit UIC, University General Hospital Consortium, Valencia, Spain Research Foundation of University General Hospital of Valencia, Valencia, Spain CIBERES, Health Institute Carlos III, Valencia, Spain Both authors contributed equally to this work
    Eur Respir J 47:1737-49. 2016
  4. pmc Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist
    Javier Milara
    Clinical Research Unit, University General Hospital Consortium, Valencia, Spain
    Respir Res 16:12. 2015
  5. doi request reprint PEG-Interferon-α ribavirin-induced HCV viral clearance: a pharmacogenetic multicenter Spanish study
    Javier Milara
    Servicio de Farmacia del Hospital General Universitario de Valencia
    Farm Hosp 39:29-43. 2015
  6. doi request reprint Mucin 1 downregulation associates with corticosteroid resistance in chronic rhinosinusitis with nasal polyps
    Javier Milara
    Clinical Research Unit, University General Hospital Consortium, Valencia, Spain Department of Biotechnology, Universidad Politecnica de Valencia, Valencia, Spain Research Foundation of General Hospital of Valencia, Valencia, Spain Electronic address
    J Allergy Clin Immunol 135:470-6. 2015
  7. doi request reprint Roflumilast N-oxide reverses corticosteroid resistance in neutrophils from patients with chronic obstructive pulmonary disease
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain Department of Biotechnology, Universidad Politecnica de Valencia, Valencia, Spain Research Foundation of General Hospital of Valencia, Valencia, Spain Electronic address
    J Allergy Clin Immunol 134:314-22. 2014
  8. doi request reprint Roflumilast N-oxide inhibits bronchial epithelial to mesenchymal transition induced by cigarette smoke in smokers with COPD
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain Department of Biotechnology, Universidad Politecnica de Valencia, Spain Research Foundation of General Hospital of Valencia, Spain Electronic address
    Pulm Pharmacol Ther 28:138-48. 2014
  9. doi request reprint Bafetinib inhibits functional responses of human eosinophils in vitro
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Av Tres Cruces s n, Valencia E 46014, Spain
    Eur J Pharmacol 715:172-80. 2013
  10. doi request reprint Epithelial to mesenchymal transition is increased in patients with COPD and induced by cigarette smoke
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain
    Thorax 68:410-20. 2013

Collaborators

Detail Information

Publications36

  1. pmc Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients
    Javier Milara
    Department of Pharmacology, Faculty of Medicine, Jaume I University, Castellon, Spain
    Respir Res 17:145. 2016
    ..In this study, neutrophils obtained from COPD patients were used to compare the anti-inflammatory effects of aclidinium bromide (a long-acting muscarinic antagonist) with corticosteroids and their potential additive effect...
  2. doi request reprint MUC4 impairs the anti-inflammatory effects of corticosteroids in patients with chronic rhinosinusitis with nasal polyps
    Javier Milara
    Department of Pharmacy, University Hospital Consortium, Valencia, Spain Department of Pharmacology, Faculty of Medicine, Jaume I University, Castellon, Spain Department of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain CIBERES, Health Institute Carlos III, Valencia, Spain Electronic address
    J Allergy Clin Immunol . 2016
    ..Moreover, MUC4β has the capacity to interact with other intracellular proteins. We hypothesized that MUC4 modulates corticosteroid efficacy of patients with CRSwNP...
  3. doi request reprint Vascular effects of sildenafil in patients with pulmonary fibrosis and pulmonary hypertension: an ex vivo/in vitro study
    Javier Milara
    Dept of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain Clinical Research Unit UIC, University General Hospital Consortium, Valencia, Spain Research Foundation of University General Hospital of Valencia, Valencia, Spain CIBERES, Health Institute Carlos III, Valencia, Spain Both authors contributed equally to this work
    Eur Respir J 47:1737-49. 2016
    ....
  4. pmc Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist
    Javier Milara
    Clinical Research Unit, University General Hospital Consortium, Valencia, Spain
    Respir Res 16:12. 2015
    ..We analyzed differential and combination anti-inflammatory effects of dexamethasone and roflumilast N-oxide in human bronchial epithelial cells (HBECs) stimulated with viral toll like receptor (TLR) agonists...
  5. doi request reprint PEG-Interferon-α ribavirin-induced HCV viral clearance: a pharmacogenetic multicenter Spanish study
    Javier Milara
    Servicio de Farmacia del Hospital General Universitario de Valencia
    Farm Hosp 39:29-43. 2015
    ..The current study analyzes host genetics, viral and clinical predictors of sustained viral response (SVR) to dual PEG-IFN and ribavirin therapy in a representative Spanish population...
  6. doi request reprint Mucin 1 downregulation associates with corticosteroid resistance in chronic rhinosinusitis with nasal polyps
    Javier Milara
    Clinical Research Unit, University General Hospital Consortium, Valencia, Spain Department of Biotechnology, Universidad Politecnica de Valencia, Valencia, Spain Research Foundation of General Hospital of Valencia, Valencia, Spain Electronic address
    J Allergy Clin Immunol 135:470-6. 2015
    ..Because glucocorticoid receptor (GR) nuclear translocation is key to the anti-inflammatory effect of corticosteroids, we hypothesized that MUC1 is involved in the effectiveness of corticosteroids...
  7. doi request reprint Roflumilast N-oxide reverses corticosteroid resistance in neutrophils from patients with chronic obstructive pulmonary disease
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain Department of Biotechnology, Universidad Politecnica de Valencia, Valencia, Spain Research Foundation of General Hospital of Valencia, Valencia, Spain Electronic address
    J Allergy Clin Immunol 134:314-22. 2014
    ..The phosphodiesterase 4 inhibitor roflumilast N-oxide (RNO) is the active metabolite of roflumilast approved as a treatment to reduce the risk of exacerbations in patients with severe COPD...
  8. doi request reprint Roflumilast N-oxide inhibits bronchial epithelial to mesenchymal transition induced by cigarette smoke in smokers with COPD
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain Department of Biotechnology, Universidad Politecnica de Valencia, Spain Research Foundation of General Hospital of Valencia, Spain Electronic address
    Pulm Pharmacol Ther 28:138-48. 2014
    ..In vitro, EMT may be reproduced by exposing well-differentiated human bronchial epithelial cells (WD-HBEC) to cigarette smoke extract (CSE). EMT may be mitigated by an increase in cellular cAMP...
  9. doi request reprint Bafetinib inhibits functional responses of human eosinophils in vitro
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Av Tres Cruces s n, Valencia E 46014, Spain
    Eur J Pharmacol 715:172-80. 2013
    ..Therefore, future anti-allergic therapies based on bafetinib, could help to suppress excessive inflammatory response of eosinophils at inflammatory sites...
  10. doi request reprint Epithelial to mesenchymal transition is increased in patients with COPD and induced by cigarette smoke
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain
    Thorax 68:410-20. 2013
    ..This study examines the EMT process in human bronchial epithelial cells (HBECs) from non-smokers, smokers and patients with COPD as well as the in vitro effect of cigarette smoke extract (CSE) on EMT...
  11. doi request reprint Aclidinium inhibits cigarette smoke-induced lung fibroblast-to-myofibroblast transition
    Javier Milara
    Research Unit, University General Hospital Consortium, Valencia, Spain
    Eur Respir J 41:1264-74. 2013
    ..A non-neuronal cholinergic system is implicated in cigarette smoke-induced bronchial fibroblast-to-myofibroblast transition, which is inhibited by aclidinium bromide...
  12. doi request reprint Bosentan inhibits cigarette smoke-induced endothelin receptor expression in pulmonary arteries
    J Milara
    Clinic Research Unit, University General Hospital Consortium, Valencia, Spain
    Eur Respir J 39:927-38. 2012
    ..These data may be of potential value to explain therapeutic effects of bosentan in some forms of disproportionate pulmonary hypertension in COPD patients...
  13. doi request reprint Neutrophil activation in severe, early-onset COPD patients versus healthy non-smoker subjects in vitro: effects of antioxidant therapy
    Javier Milara
    Research Unit, University General Hospital Consortium, and Department of Medicine, Faculty of Medicine, University of Valencia, Valencia, Spain
    Respiration 83:147-58. 2012
    ..Severe, early-onset COPD is characterized by a rapid decline in the lung function at an early age; however, nothing is known about neutrophil activation in COPD patients...
  14. pmc Aclidinium inhibits human lung fibroblast to myofibroblast transition
    Javier Milara
    Unidad de Investigacion, Consorcio Hospital General, Universitario, Avenida Tres Vruces s n, Valencia E 46014, Spain
    Thorax 67:229-37. 2012
    ..This study examines the role of aclidinium, a new long-acting muscarinic antagonist, on human fibroblast to myofibroblast transition...
  15. doi request reprint Oxidative stress-induced glucocorticoid resistance is prevented by dual PDE3/PDE4 inhibition in human alveolar macrophages
    J Milara
    Research Unit, University General Hospital Consortium, Valencia, Spain
    Clin Exp Allergy 41:535-46. 2011
    ..Oxidative stress is present in airway diseases such as severe asthma or Chronic Obstructive Pulmonary Disease and contributes to the low response to glucocorticoids through the down-regulation of histone deacetylase (HDAC) activity...
  16. doi request reprint Extracellular calcium-sensing receptor mediates human bronchial epithelial wound repair
    Javier Milara
    University General Hospital Consortium, Valencia, Spain
    Biochem Pharmacol 80:236-46. 2010
    ..Our data, for the first time, shows that CaSR plays an important role in airway epithelial repair, which may help to develop novel regenerative therapeutics allowing the rapid repair of lung damaged epithelium...
  17. doi request reprint Cigarette smoke-induced pulmonary endothelial dysfunction is partially suppressed by sildenafil
    Javier Milara
    Research Unit, University General Hospital Consortium, Valencia, Spain
    Eur J Pharm Sci 39:363-72. 2010
    ....
  18. pmc Cigarette smoke exposure up-regulates endothelin receptor B in human pulmonary artery endothelial cells: molecular and functional consequences
    J Milara
    Research Unit, University General Hospital Consortium, Valencia, Spain
    Br J Pharmacol 161:1599-615. 2010
    ..We studied the effect of cigarette smoke extract (CSE) on endothelin receptor B (ET(B) ) expression in human pulmonary artery endothelial cells (HPAECs) and its role in endothelial dysfunction...
  19. doi request reprint Role of adenylate kinase type 7 expression on cilia motility: possible link in primary ciliary dyskinesia
    Javier Milara
    Research Unit, University General Hospital Consortium, Avenida Tres Cruces s n, Valencia, Spain
    Am J Rhinol Allergy 24:181-5. 2010
    ....
  20. doi request reprint Sphingosine-1-phosphate increases human alveolar epithelial IL-8 secretion, proliferation and neutrophil chemotaxis
    Javier Milara
    Department of Pharmacology, Faculty of Medicine, University of Valencia, Spain
    Eur J Pharmacol 609:132-9. 2009
    ..This study demonstrates for the first time that S1P participates in the alveolar epithelial interactions in vitro...
  21. doi request reprint Sphingosine-1-phosphate is increased in patients with idiopathic pulmonary fibrosis and mediates epithelial to mesenchymal transition
    Javier Milara
    Research Foundation, University General Hospital Consortium, Valencia, Spain
    Thorax 67:147-56. 2012
    ..Sphingosine-1-phosphate (S1P) and sphingosine kinase 1 (SPHK1) have been implicated in lung myofibroblast transition, but their role in EMT and their expression in patients with IPF is unknown...
  22. doi request reprint Nickel induces intracellular calcium mobilization and pathophysiological responses in human cultured airway epithelial cells
    Julio Cortijo
    Department of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain
    Chem Biol Interact 183:25-33. 2010
    ....
  23. doi request reprint Direct effect of cigarette smoke on human pulmonary artery tension
    Jose Luis Ortiz
    Department of Pharmacology, Faculty of Medicine, University of Valencia, Spain
    Pulm Pharmacol Ther 23:222-8. 2010
    ....
  24. doi request reprint Cilia motility and structure in primary and secondary ciliary dyskinesia
    Miguel Armengot
    Rhinology Unit, General and University Hospital, Medical School, Valencia University, Valencia, Spain
    Am J Rhinol Allergy 24:175-80. 2010
    ..The aim of this work was to correlate nasal ciliary activity with clinical and structural abnormalities in PCD. A secondary aim was to evaluate the usefulness of high-resolution digital high-speed video (DHSV) in the diagnosis of PCD...
  25. doi request reprint Simvastatin Increases the Ability of Roflumilast N-oxide to Inhibit Cigarette Smoke-Induced Epithelial to Mesenchymal Transition in Well-differentiated Human Bronchial Epithelial Cells in vitro
    Javier Milara
    1Clinical Research Unit UIC, University General Hospital Consortium, Valencia, Spain
    COPD 12:320-31. 2015
    ..Further, statins were shown to protect renal and alveolar epithelial cells from EMT...
  26. pmc Nasal ciliary beat frequency and beat pattern in retinal ciliopathies
    Miguel Armengot
    Rhinology Unit, General and University Hospital, Medical School, Valencia University, Valencia, Spain
    Invest Ophthalmol Vis Sci 53:2076-9. 2012
    ..The purpose of this study was to evaluate the nasal ciliary beat frequency and beat pattern in patients with retinitis pigmentosa (RP) and Usher syndrome type II and compare it with that of healthy control subjects...
  27. pmc Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECT in Guinea Pig: Role of Bitter Taste Agonists
    Jose Luis Ortiz
    Department of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain
    PLoS ONE 11:e0164399. 2016
    ..T2R agonists increased CBF in human nasal ciliated cells in vitro and induced bronchodilation in human bronchi ex vivo. In summary, T2R agonists increase MCC in vivo as assessed by 3D micro-CT-SPECT analysis...
  28. ncbi request reprint Tobacco, inflammation, and respiratory tract cancer
    Javier Milara
    Clinical research unit UIC, University General Hospital Consortium, Valencia, Spain
    Curr Pharm Des 18:3901-38. 2012
    ..In this review we focused in current knowledge on the mechanisms implicated in cigarette smoke-induced lung chronic inflammatory processes leading to lung carcinogenesis, as well as in current therapies based on novel molecular advances...
  29. pmc Study of USH1 splicing variants through minigenes and transcript analysis from nasal epithelial cells
    María José Aparisi
    Research Group on Sensorineural Diseases, Instituto de Investigación Sanitaria La Fe, Valencia, Spain
    PLoS ONE 8:e57506. 2013
    ..In addition, we could observe that the nasal ciliated epithelium of USH1 patients shows a lower ciliary beat frequency than control subjects...
  30. doi request reprint Hawthorn extract inhibits human isolated neutrophil functions
    Ernesto Dalli
    Service of Cardiology, Associated University Hospital Arnau de Vilanova, Valencia, Spain
    Pharmacol Res 57:445-50. 2008
    ..In conclusion, C. laevigata extract inhibited various functional outputs of activated human neutrophils which may be relevant to the pathophysiology of cardiac failure...
  31. doi request reprint Evaluation of the Ocular Tolerance of Three Tacrolimus Topical Pharmaceutical Preparations by Bovine Corneal Opacity and Permeability Test
    Alfonso Pastor-Clerigues
    a Research Foundation of General Hospital of Valencia, Valencia, Spain
    Curr Eye Res 41:890-6. 2016
    ..In this study, the local corneal tolerance and safety profile of three common tacrolimus 0.03% pharmaceutical preparations were evaluated...
  32. doi request reprint Role of tetrahydrobiopterin in pulmonary vascular remodelling associated with pulmonary fibrosis
    Patricia Almudever
    Department of Pharmacology, Faculty of Medicine, University of Valencia, Valencia, Spain
    Thorax 68:938-48. 2013
    ....
  33. pmc Roflumilast Prevents the Metabolic Effects of Bleomycin-Induced Fibrosis in a Murine Model
    Javier Milara
    Clinical Research Unit, University General Hospital Consortium, Valencia, Spain CIBERES, Health Institute Carlos III, Valencia, Spain Research Foundation of General Hospital of Valencia, Av Tres Cruces s n, E 46014, Valencia, Spain
    PLoS ONE 10:e0133453. 2015
    ..Roflumilast prevented most of these metabolic effects associated to pulmonary fibrosis suggesting a favorable anti-fibrotic profile. ..
  34. doi request reprint Relaxation by beta 3-adrenoceptor agonists of the isolated human internal anal sphincter
    Cristina Ballester
    Department of Surgery, University of Valencia, Valencia, Spain
    Life Sci 86:358-64. 2010
    ..In this study, responses of beta(3)-adrenoceptor agonists were examined on human isolated internal anal sphincter (IAS) in order to explore their relaxant effects on hypertonicity of IAS...
  35. pmc Anti-inflammatory and anti-fibrotic profile of fish oil emulsions used in parenteral nutrition-associated liver disease
    Alfonso Pastor-Clerigues
    Hospital Pharmacy, Clinical Nutrition Unit, University General Hospital Consortium, Valencia, Spain
    PLoS ONE 9:e115404. 2014
    ..In other experiments, TGFβ1 induced EMT that was suppressed by Omegaven® and enhanced by soybean oil lipid emulsions. Omegaven® improves clinical, anti-inflammatory and anti-fibrotic parameters in adults with long-term home PNALD. ..
  36. doi request reprint Effects of long-term azithromycin therapy on airway oxidative stress markers in non-cystic fibrosis bronchiectasis
    Afredo De Diego
    Department of Pneumology, University Hospital La Fe, Valencia, Spain
    Respirology 18:1056-62. 2013
    ..To explore the effect of long-term therapy with azithromycin in regards to airway oxidative stress markers in exhaled breath condensate (EBC) of adult patients with stable non-cystic fibrosis (CF) bronchiectasis...