- Exploiting molecular virulence determinants in Burkholderia to develop vaccine antigens
William Thomas Casey
Centre of Microbial Host Interactions, Institute of Technology Tallaght, Old Blessington Road, Dublin 24, Ireland
Curr Med Chem 22:1719-33. 2015
..Better integration of the knowledge on the pathogenesis and molecular determinants of virulence for these Burkholderia spp may allow the development of more efficacious vaccines. ..
- Eight stranded β -barrel and related outer membrane proteins: role in bacterial pathogenesis
Centre of Microbial Host Interactions and Centre of Applied Science for Health, Institute of Technology, Tallaght, Dublin 24, Ireland
Protein Pept Lett 19:1013-25. 2012
..The role of these proteins in pathogenesis is discussed here, together with the potential for these proteins to be used as immunoprophylactic agents to protect against infection...
- Burkholderia cepacia complex: epithelial cell-pathogen confrontations and potential for therapeutic intervention
Centre of Microbial Host Interactions, Institute of Technology Tallaght Dublin, Dublin 24, Ireland
J Med Microbiol 58:1-12. 2009
..This review outlines these interactions and examines the therapeutic implications of understanding the mechanisms of pathogenesis of this difficult, antibiotic-resistant, opportunistic pathogen...
- Melittin as a permeability enhancer II: in vitro investigations in human mucus secreting intestinal monolayers and rat colonic mucosae
ITT Dublin, Belgard Road, Tallaght, Dublin, Ireland
Pharm Res 24:1346-56. 2007
..Our objectives were to assess in vitro efficacy and cytotoxicity of melittin in two intestinal permeability models and investigate the potential mechanism by which melittin might enhance gastrointestinal absorption...
- Differences in invasion and translocation of Burkholderia cepacia complex species in polarised lung epithelial cells in vitro
Department of Applied Science, Institute of Technology, Tallaght, Ireland
Microb Pathog 41:183-92. 2006
- Inhibition of Burkholderia multivorans adhesion to lung epithelial cells by bivalent lactosides
Centre of Microbial Host Interactions, Institute of Technology Tallaght Dublin, Belgard Road, Tallaght, Dublin 24, Ireland
Molecules 17:10065-71. 2012
..Development of glycoconjugate therapies such as these, which inhibit attachment to lung epithelial cells, represent an alternative means of preventing infection with inherently antimicrobially resistant pathogens such as B. multivorans...
- Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells
Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland
J Med Microbiol 60:289-99. 2011
..In conclusion, whilst multiple mechanisms of pathogenicity may exist across the genus Pandoraea, it appears that lung cell invasion and translocation contribute to the virulence of P. pulmonicola strains...
- Hypoxia modulates infection of epithelial cells by Pseudomonas aeruginosa
UCD School of Medicine and Medical Science and The Conway Institute, University College Dublin, Dublin, Ireland
PLoS ONE 8:e56491. 2013
..Thus, hypoxia reduces P. aeruginosa internalization into epithelial cells and pharmacologic manipulation of the host pathways involved may represent new therapeutic targets in the treatment of P. aeruginosa infection...
- Activation of MMP-9 by human lung epithelial cells in response to the cystic fibrosis-associated pathogen Burkholderia cenocepacia reduced wound healing in vitro
Centre of Microbial Host Interactions, Institute of Technology Tallaght, Dublin, Ireland
Am J Physiol Lung Cell Mol Physiol 301:L575-86. 2011
..cenocepacia infection and that its activation following B. cenocepacia infection contributes to the pathogenesis of this virulent pathogen...
- IL-8 released from human lung epithelial cells induced by cystic fibrosis pathogens Burkholderia cepacia complex affects the growth and intracellular survival of bacteria
Seshu K Kaza
Centre for Microbial Host Interactions, Department of Science, ITT Dublin, Dublin 24, Ireland
Int J Med Microbiol 301:26-33. 2011
- Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells
Department of Applied Science and National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland
J Med Microbiol 57:15-20. 2008
..This property is likely to be a major factor in the pathogenesis of the genus...
- Melittin exhibits necrotic cytotoxicity in gastrointestinal cells which is attenuated by cholesterol
Institute of Technology Tallaght Dublin and National Institute of Cellular Biotechnology, Old Blessington Road, Tallaght, Dublin 24, Ireland
Biochem Pharmacol 75:1104-14. 2008
..Overall, although melittin appears to stimulate necrosis, with careful dosage selection the peptide could be considered for the oral delivery of poorly bioavailable drugs...
- The effect of recombinant human lactoferrin on growth and the antibiotic susceptibility of the cystic fibrosis pathogen Burkholderia cepacia complex when cultured planktonically or as biofilms
Emma M Caraher
Centre of Microbial Host Interaction, Department of Science, Institute of Technology Tallaght ITT Dublin, Tallaght, Dublin 24, Ireland
J Antimicrob Chemother 60:546-54. 2007
- Bis-phenanthroline copper(II) phthalate complexes are potent in vitro antitumour agents with 'self-activating' metallo-nuclease and DNA binding properties
The inorganic pharmaceutical and biomimetic research centre, Focas Research Institute, Dublin Institute of Technology, Camden Row, Dublin, 8, Ireland
Dalton Trans 40:1024-7. 2011
..The complexes induce relaxation of supercoiled plasmid DNA in the absence of external reducing agents and display efficient CT-DNA, Poly[d(A-T)](2) and Poly[d(G-C)](2) binding...
- Invasion of Burkholderia cepacia complex isolates into lung epithelial cells involves glycolipid receptors
Centre of Microbial Host Interactions, Institute of Technology, Tallaght, Dublin 24, Ireland
Microb Pathog 49:381-7. 2010
..Although the specific glycolipid involved in each case has not been elucidated, it is apparent that invasion of lung epithelial cells is mediated via binding to glycosphingolipid receptors...
- Melittin as an epithelial permeability enhancer I: investigation of its mechanism of action in Caco-2 monolayers
Institute of Technology Tallaght Dublin, Belgard Road, Tallaght, Dublin, Ireland
Pharm Res 24:1336-45. 2007
..The aim of this work was to further examine the potential of melittin as a paracellular permeability enhancer and to investigate the mechanism of interaction with tight junction proteins in Caco-2...
- Immunoproteomic analysis of proteins expressed by two related pathogens, Burkholderia multivorans and Burkholderia cenocepacia, during human infection
Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin, Ireland
PLoS ONE 8:e80796. 2013
..In addition, identification of some of these could also allow the development of protective vaccines which may prevent colonisation. ..
- Mast cell tryptase reduces junctional adhesion molecule-A (JAM-A) expression in intestinal epithelial cells: implications for the mechanisms of barrier dysfunction in irritable bowel syndrome
Ewa M Wilcz-Villega
Department of Clinical Medicine, Adelaide and Meath Hospital, Dublin, Ireland
Am J Gastroenterol 108:1140-51. 2013
..The objective of this study was to investigate how mast cell tryptase may influence intestinal permeability and tight junction (TJ) proteins in vitro and explore translation to irritable bowel syndrome (IBS)...
- Bacterial host interactions in cystic fibrosis
Centre of Microbial Host Interactions and Centre of Applied Science for Health, Institute of Technology Tallaght, Tallaght, Dublin 24, Ireland
Curr Opin Microbiol 15:71-7. 2012
..Hypermutability is common among CF pathogens facilitating adaptation and as the host response persists, progressive destruction of the normal architecture of lung tissue ensues with catastrophic consequences for the host...
- Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans
Department of Applied Science, National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland
J Cyst Fibros 6:49-56. 2007
..The two most common species among the CF community are Burkholderia cenocepacia and Burkholderia multivorans. However, Burkholderia dolosa has recently been causing concern due to its transmissibility and virulence in CF patients...
- Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans
Centre of Microbial Host Interactions, Centre of Applied Science for Health, Institute of Technology Tallaght, Old Blessington Road, Tallaght, Dublin 24, Ireland
Microbiology 160:1474-87. 2014
..aeruginosa growth. B. multivorans also inhibited B. cenocepacia and Pa. apista. In conclusion, both Ps. aeruginosa and B. multivorans are capable of suppressing growth and virulence of co-colonizing CF pathogens. ..
- Macrophage responses to CF pathogens: JNK MAP kinase signaling by Burkholderia cepacia complex lipopolysaccharide
Centre of Microbial Host Interactions, ITT Dublin, Dublin, Ireland
FEMS Immunol Med Microbiol 60:36-43. 2010
..These studies further characterize the host response to Bcc and in particular B. multivorans, now the predominant Bcc species in many CF populations...
- Spectroscopic studies, DFT calculations, and cytotoxic activity of novel silver(I) complexes of hydroxy ortho-substituted-nitro-2H-chromen-2-one ligands and a phenanthroline adduct
Centre of Applied Science for Health, Institute of Technology Tallaght, Dublin 24, Ireland
J Inorg Biochem 153:103-13. 2015
- Immunoproteomics: the key to discovery of new vaccine antigens against bacterial respiratory infections
Centre of Microbial Host Interactions, Centre of Applied Science for Health, Institute of Technology Tallaght, Old Blessington Road, Dublin 24, Ireland
Curr Protein Pept Sci 13:807-15. 2012
..Although only one step in the pathway towards an efficacious approved vaccine, immunoproteomics is an important technology in the identification of novel vaccine antigens...
- Biological activity and coordination modes of copper(II) complexes of Schiff base-derived coumarin ligands
Bernadette Sarah Creaven
Department of Science, Institute of Technology Tallaght, Dublin 24, Ireland
Dalton Trans 39:10854-65. 2010
- Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro
Institute of Technology Tallaght Dublin, Old Blessington Road, Tallaght, Dublin 24, Republic of Ireland
Biochem Pharmacol 71:1289-98. 2006
..Melittin, while showing little potential as an antimicrobial agent, may have potential in delivery of poorly bioavailable drugs...