Hyungdon Yun

Summary

Affiliation: Yeungnam University
Country: Korea

Publications

  1. pmc Engineering of daidzein 3'-hydroxylase P450 enzyme into catalytically self-sufficient cytochrome P450
    Kwon Young Choi
    School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea
    Microb Cell Fact 11:81. 2012
  2. doi request reprint One-pot one-step deracemization of amines using ω-transaminases
    Giyoung Shin
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Chem Commun (Camb) 49:8629-31. 2013
  3. doi request reprint Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase
    Young Man Seo
    School of Biotechnology, Yeungnam University, Gyeongbuk, 712 749, South Korea
    Org Biomol Chem 10:2482-5. 2012
  4. doi request reprint Biosynthetic substitution of tyrosine in green fluorescent protein with its surrogate fluorotyrosine in Escherichia coli
    Niraikulam Ayyadurai
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    Biotechnol Lett 33:2201-7. 2011
  5. pmc Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae
    Pradeepraj Durairaj
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    Microb Cell Fact 14:45. 2015
  6. doi request reprint Enzymatic synthesis of chiral γ-amino acids using ω-transaminase
    Minsu Shon
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Chem Commun (Camb) 50:12680-3. 2014
  7. pmc Crystallization and preliminary X-ray crystallographic studies of β-transaminase from Mesorhizobium sp. strain LUK
    Bokyung Kim
    School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:231-3. 2011
  8. doi request reprint Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications
    Yuvaraj Ravikumar
    School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan, Gyeongbuk, Korea
    Biotechnol J 10:1862-76. 2015
  9. doi request reprint Asymmetric synthesis of aromatic β-amino acids using ω-transaminase: Optimizing the lipase concentration to obtain thermodynamically unstable β-keto acids
    Sam Mathew
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Biotechnol J 11:185-90. 2016
  10. doi request reprint FMN-Based Fluorescent Proteins as Heavy Metal Sensors Against Mercury Ions
    Yuvaraj Ravikumar
    School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea
    J Microbiol Biotechnol 26:530-9. 2016

Collaborators

  • Byung Gee Kim
  • Sam Mathew
  • Yong Ho Khang
  • Hyung Joon Cha
  • Sun Gu Lee
  • Saravanan Prabhu Nadarajan
  • Yuvaraj Ravikumar
  • Niraikulam Ayyadurai
  • Kanagavel Deepankumar
  • Young Man Seo
  • Pradeepraj Durairaj
  • Chong Soon Lee
  • Eunok Jung
  • Minsu Shon
  • Giyoung Shin
  • Han Seop Bea
  • Nadarajan Saravanan Prabhu
  • Tae Hyeon Yoo
  • Hyun Ho Park
  • Kwon Young Choi
  • Dong Ho Bae
  • Seunho Jung
  • Sailesh Malla
  • Pyung Gang Lee
  • Ramachandran Shanmugavel
  • Byeo Ri An
  • Hyung Yeon Park
  • Changmin Sung
  • Da Hye Jung
  • Bishnu Prasad Pandey
  • Nataraj Chitrapriya
  • Yoon Jung Jang
  • Aran Kim
  • Nagasundarapandian Soundrarajan
  • Seog K Kim
  • Eunjung Song
  • Nahum Lee
  • Nediljko Budisa
  • Hye Jeong Park

Detail Information

Publications21

  1. pmc Engineering of daidzein 3'-hydroxylase P450 enzyme into catalytically self-sufficient cytochrome P450
    Kwon Young Choi
    School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea
    Microb Cell Fact 11:81. 2012
    ..Finally, a recombinant Streptomyces avermitilis host for the expression of 3'-ASDH and production of the hydroxylated product was developed. The conversion that was attained (34.6%) was 5.2-fold higher than that of the wild-type...
  2. doi request reprint One-pot one-step deracemization of amines using ω-transaminases
    Giyoung Shin
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Chem Commun (Camb) 49:8629-31. 2013
    ..Using this method, various aromatic amines were successfully converted to their (R)-forms (>99%) with good conversion. ..
  3. doi request reprint Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase
    Young Man Seo
    School of Biotechnology, Yeungnam University, Gyeongbuk, 712 749, South Korea
    Org Biomol Chem 10:2482-5. 2012
    ..A whole cell reaction using a biphasic system converted 500 mM racemic homoalanine to 485 mM L-homoalanine (>99% ee)...
  4. doi request reprint Biosynthetic substitution of tyrosine in green fluorescent protein with its surrogate fluorotyrosine in Escherichia coli
    Niraikulam Ayyadurai
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    Biotechnol Lett 33:2201-7. 2011
    ....
  5. pmc Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae
    Pradeepraj Durairaj
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    Microb Cell Fact 14:45. 2015
    ..Cytochrome P450, the ubiquitous enzyme is capable of catalyzing the selective terminal omega hydroxylation naturally in biological kingdom...
  6. doi request reprint Enzymatic synthesis of chiral γ-amino acids using ω-transaminase
    Minsu Shon
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Chem Commun (Camb) 50:12680-3. 2014
    ..The present study demonstrates the high potentiality of ω-TA reaction for the production of chiral γ-amino acids. ..
  7. pmc Crystallization and preliminary X-ray crystallographic studies of β-transaminase from Mesorhizobium sp. strain LUK
    Bokyung Kim
    School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:231-3. 2011
    ..X-ray diffraction data were collected to a resolution of 2.5 Å from a crystal that belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 90.91, b = 192.17, c = 52.75 Å...
  8. doi request reprint Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications
    Yuvaraj Ravikumar
    School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan, Gyeongbuk, Korea
    Biotechnol J 10:1862-76. 2015
    ..Considering the potential of this tool and by exploiting these engineered enzymes, we expect the field of bioprocess engineering to open up new opportunities for biocatalysis in the near future. ..
  9. doi request reprint Asymmetric synthesis of aromatic β-amino acids using ω-transaminase: Optimizing the lipase concentration to obtain thermodynamically unstable β-keto acids
    Sam Mathew
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Biotechnol J 11:185-90. 2016
    ..This strategy can be used as a general approach to efficiently generate β-amino acids from β-ketoesters. ..
  10. doi request reprint FMN-Based Fluorescent Proteins as Heavy Metal Sensors Against Mercury Ions
    Yuvaraj Ravikumar
    School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea
    J Microbiol Biotechnol 26:530-9. 2016
    ..1 to 3 µM of Hg(2+). Thus, further engineering with a rational approach would enable the FbFP to be developed as a novel and highly selective and sensitive biosensor for other toxic heavy metal ions as well. ..
  11. doi request reprint An in silico approach to evaluate the polyspecificity of methionyl-tRNA synthetases
    Saravanan Prabhu Nadarajan
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    J Mol Graph Model 39:79-86. 2013
    ..Given the close similarity in the active site residues of these systems, we evaluated the polyspecificity of MetRSs...
  12. doi request reprint Kinetic resolution of aromatic β-amino acids by ω-transaminase
    Han Seop Bea
    School of Biotechnology, Yeungnam University, Gyeongbuk, 712 749, Korea
    Chem Commun (Camb) 47:5894-6. 2011
    ..Racemic aromatic β-amino acids have been kinetically resolved into (R)-β-amino acids with high enantiomeric excess (>99%) by a novel ω-TA with ca. 50% conversion...
  13. ncbi request reprint Kinetic resolution of 3-fluoroalanine using a fusion protein of D-amino acid oxidase with Vitroscilla hemoglobin
    Young Man Seo
    School of Biotechnology, Yeungnam University, Japan
    Biosci Biotechnol Biochem 75:820-2. 2011
    ..9 s⁻¹). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion...
  14. doi request reprint Enhancing the biophysical properties of mRFP1 through incorporation of fluoroproline
    Kanagavel Deepankumar
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    Biochem Biophys Res Commun 440:509-14. 2013
    ..Our study showed that a successful enhancement in the biophysical property of mRFP1-P63A[(4R)-FP] using non-canonical amino acid mutagenesis after mutating non-permissive site Pro63 into Ala. ..
  15. doi request reprint A facile and efficient method for the incorporation of multiple unnatural amino acids into a single protein
    Niraikulam Ayyadurai
    School of Biotechnology, Yeungnam University, South Korea
    Chem Commun (Camb) 47:3430-2. 2011
    ..This single protein exhibited two different novel functionalities acquired from the genetically incorporated unnatural amino acids, which is an interesting and not an inherent property of the protein...
  16. doi request reprint Bioconjugation of L-3,4-dihydroxyphenylalanine containing protein with a polysaccharide
    Niraikulam Ayyadurai
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    Bioconjug Chem 22:551-5. 2011
    ..This novel approach holds great promise for widespread use to prepare protein conjugates and synthetic biology applications...
  17. ncbi request reprint Enzymatic synthesis of L-tert-leucine with branched chain aminotransferase
    Young Man Seo
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712 749, Korea
    J Microbiol Biotechnol 21:1049-52. 2011
    ..In the BCAT/ AspAT/PDC coupling reaction, 89.2 mM L-tert-leucine (ee >99%) was asymmetrically synthesized from 100 mM trimethylpyruvate...
  18. ncbi request reprint Asymmetric synthesis of (S)-alpha-methylbenzylamine by recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase
    Hyungdon Yun
    School of Biotechnology, Yeungnam University, Gyeongbuk, Korea
    Biosci Biotechnol Biochem 72:3030-3. 2008
    ..5-fold, and the inhibitory (S)-alpha-MBA produced in the omega-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent...
  19. doi request reprint Comparative functional characterization of a novel benzoate hydroxylase cytochrome P450 of Fusarium oxysporum
    Pradeepraj Durairaj
    School of Biotechnology, Yeungnam University, Gyeongsan, South Korea
    Enzyme Microb Technol 70:58-65. 2015
    ..The electron transfer compatibility and the coupling efficiency between the homologous FoCYP-FoCPR system are significant and it favored enhanced monooxygenase activity with broader substrate specificity. ..
  20. doi request reprint Production of chiral β-amino acids using ω-transaminase from Burkholderia graminis
    Sam Mathew
    School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, South Korea
    J Biotechnol 196:1-8. 2015
    ..Additionally, kinetic resolution of aromatic β-amino acids was performed using benzaldehyde as a cheap amino acceptor...
  21. doi request reprint Unnatural amino acid mutagenesis-based enzyme engineering
    Yuvaraj Ravikumar
    School of Biotechnology, Department of Biochemistry, Yeungnam University, Gyeongsan, Gyeongbuk 712 749, Korea
    Trends Biotechnol 33:462-70. 2015
    ..We describe the contribution of in vivo UAA incorporation methodologies to enzyme engineering as well as the future prospects for the field, including the integration of UAAs with other new advances in enzyme engineering. ..