Chihiro Sasakawa

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. pmc Interaction of CagA with Crk plays an important role in Helicobacter pylori-induced loss of gastric epithelial cell adhesion
    Masato Suzuki
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    J Exp Med 202:1235-47. 2005
  2. pmc NF-kappaB activation by Helicobacter pylori requires Akt-mediated phosphorylation of p65
    Eriko Takeshima
    Division of Molecular Virology and Oncology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan
    BMC Microbiol 9:36. 2009
  3. pmc A new paradigm of bacteria-gut interplay brought through the study of Shigella
    Chihiro Sasakawa
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:229-43. 2010
  4. doi request reprint The Shigella OspC3 effector inhibits caspase-4, antagonizes inflammatory cell death, and promotes epithelial infection
    Taira Kobayashi
    Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Cell Host Microbe 13:570-83. 2013
  5. doi request reprint Helicobacter pylori CagA phosphorylation-independent function in epithelial proliferation and inflammation
    Masato Suzuki
    Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell Host Microbe 5:23-34. 2009
  6. doi request reprint The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response
    Takahito Sanada
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Nature 483:623-6. 2012
  7. pmc Reinforcement of epithelial cell adhesion to basement membrane by a bacterial pathogen as a new infectious stratagem
    Minsoo Kim
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, Tokyo, Japan
    Virulence 1:52-5. 2010
  8. pmc A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response
    Hiroshi Ashida
    Department of Microbiology and Immunology, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Cell Biol 12:66-73; sup pp 1-9. 2010
  9. ncbi request reprint Helicobacter pylori dampens gut epithelial self-renewal by inhibiting apoptosis, a bacterial strategy to enhance colonization of the stomach
    Hitomi Mimuro
    Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
    Cell Host Microbe 2:250-63. 2007
  10. doi request reprint Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment
    Minsoo Kim
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nature 459:578-82. 2009

Detail Information

Publications72

  1. pmc Interaction of CagA with Crk plays an important role in Helicobacter pylori-induced loss of gastric epithelial cell adhesion
    Masato Suzuki
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    J Exp Med 202:1235-47. 2005
    ..Thus, CagA targeting of Crk plays a central role in inducing the pleiotropic cell responses to H. pylori infection that cause several gastric diseases, including gastric cancer...
  2. pmc NF-kappaB activation by Helicobacter pylori requires Akt-mediated phosphorylation of p65
    Eriko Takeshima
    Division of Molecular Virology and Oncology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan
    BMC Microbiol 9:36. 2009
    ..We examined whether H. pylori activates Akt in gastric epithelial cells, the role of cag PAI in this process and the role of Akt in regulating H. pylori-induced NF-kappaB activation...
  3. pmc A new paradigm of bacteria-gut interplay brought through the study of Shigella
    Chihiro Sasakawa
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:229-43. 2010
    ..Though bacterial stratagems and the molecular mechanisms of infection vary greatly among pathogens, the current studies of Shigella provide a paradigm shift in bacterial pathogenesis...
  4. doi request reprint The Shigella OspC3 effector inhibits caspase-4, antagonizes inflammatory cell death, and promotes epithelial infection
    Taira Kobayashi
    Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Cell Host Microbe 13:570-83. 2013
    ..These findings highlight the importance of caspase-4-dependent innate immune responses and demonstrate that Shigella delivers a caspase-4-specific inhibitor to delay epithelial cell death and promote infection...
  5. doi request reprint Helicobacter pylori CagA phosphorylation-independent function in epithelial proliferation and inflammation
    Masato Suzuki
    Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell Host Microbe 5:23-34. 2009
    ..Thus, nonphosphorylated CagA activity contributes to the epithelial proliferative and proinflammatory responses associated with development of chronic gastritis and gastric cancer...
  6. doi request reprint The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response
    Takahito Sanada
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Nature 483:623-6. 2012
    ..Our results suggest that S. flexneri inhibits acute inflammatory responses in the initial stage of infection by targeting the UBC13-TRAF6 complex...
  7. pmc Reinforcement of epithelial cell adhesion to basement membrane by a bacterial pathogen as a new infectious stratagem
    Minsoo Kim
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, Tokyo, Japan
    Virulence 1:52-5. 2010
    ..The findings thus establish a new class of virulence-associated factors, and provide new insight into the functioning of the intestinal barrier and bacterial strategies for circumventing it...
  8. pmc A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response
    Hiroshi Ashida
    Department of Microbiology and Immunology, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Cell Biol 12:66-73; sup pp 1-9. 2010
    ..As NEMO is essential for NF-kappaB activation, we propose that the polyubiquitylation and degradation of NEMO during Shigella infection is a new bacterial strategy to modulate host inflammatory responses...
  9. ncbi request reprint Helicobacter pylori dampens gut epithelial self-renewal by inhibiting apoptosis, a bacterial strategy to enhance colonization of the stomach
    Hitomi Mimuro
    Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
    Cell Host Microbe 2:250-63. 2007
    ..Thus, CagA activates host cell survival and antiapoptotic pathways to overcome self-renewal of the gastric epithelium and help sustain Hp infection...
  10. doi request reprint Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment
    Minsoo Kim
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nature 459:578-82. 2009
    ..These results indicate that Shigella sustain their infectious foothold by using special tactics to prevent detachment of infected cells...
  11. doi request reprint Shigella are versatile mucosal pathogens that circumvent the host innate immune system
    Hiroshi Ashida
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Curr Opin Immunol 23:448-55. 2011
    ..Here we review current topics on host defense mechanisms against bacterial infection and the countermeasures that Shigella use to evade the innate immune system...
  12. pmc Attenuated CagA oncoprotein in Helicobacter pylori from Amerindians in Peruvian Amazon
    Masato Suzuki
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Biol Chem 286:29964-72. 2011
    ....
  13. doi request reprint A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens
    Michinaga Ogawa
    Division of Bacterial Infection, The University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell Host Microbe 9:376-89. 2011
    ..Further, depolarized mitochondria and misfolded protein aggregates accumulated in the Tecpr1-knockout MEFs. Thus, we identify a Tecpr1-dependent pathway as important in targeting bacterial pathogens for selective autophagy...
  14. pmc BabA-mediated adherence is a potentiator of the Helicobacter pylori type IV secretion system activity
    Nozomi Ishijima
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 286:25256-64. 2011
    ..pylori can exploit BabA-Le(b) binding to trigger TFSS-dependent host cell signaling to induce the transcription of genes that enhance inflammation, development of intestinal metaplasia, and associated precancerous transformations...
  15. doi request reprint Listeria monocytogenes ActA-mediated escape from autophagic recognition
    Yuko Yoshikawa
    Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Cell Biol 11:1233-40. 2009
    ..Our findings indicate that ActA-mediated host protein recruitment is a unique bacterial disguise tactic to escape from autophagy...
  16. ncbi request reprint Targeting of enteropathogenic Escherichia coli EspF to host mitochondria is essential for bacterial pathogenesis: critical role of the 16th leucine residue in EspF
    Takeshi Nagai
    Department of Microbiology and Immunity, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 280:2998-3011. 2005
    ..These results indicate that EspF activity contributes to bacterial pathogenesis, as judged by murine lethality and intestinal histopathology, and promotion of bacterial colonization of the intestinal mucosa...
  17. pmc Cell death and infection: a double-edged sword for host and pathogen survival
    Hiroshi Ashida
    Department of Microbiology and Immunology, International Research Center for Infectious Disease, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Cell Biol 195:931-42. 2011
    ..Understanding the molecular details by which bacterial pathogens manipulate cell death pathways will provide insight into new therapeutic approaches to control infection...
  18. doi request reprint Control of epithelial cell structure and developmental fate: lessons from Helicobacter pylori
    Hitomi Mimuro
    Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Bioessays 30:515-20. 2008
    ..This research contributes to understanding infectious disease and the control of cell fates...
  19. ncbi request reprint IcsB, secreted via the type III secretion system, is chaperoned by IpgA and required at the post-invasion stage of Shigella pathogenicity
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Mol Microbiol 48:913-31. 2003
    ..These results suggest that IcsB is secreted via the TTSS, chaperoned by IpgA, and required at the post-invasion stage of Shigella pathogenicity..
  20. pmc Increased adherence to Caco-2 cells caused by disruption of the yhiE and yhiF genes in enterohemorrhagic Escherichia coli O157:H7
    Ichiro Tatsuno
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Infect Immun 71:2598-606. 2003
    ..These results suggest that yhiE and yhiF are involved in the adherence of O157Sakai to epithelial cells as negative regulators for the expression of the genes required for the type III secretion system...
  21. ncbi request reprint Structural definition on the surface of Helicobacter pylori type IV secretion apparatus
    Jiro Tanaka
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Cell Microbiol 5:395-404. 2003
    ..These results strongly suggest that the H. pylori TFSS apparatus is a filamentous macromolecular structure protruding from the bacterial envelope...
  22. pmc Shigella deliver an effector protein to trigger host microtubule destabilization, which promotes Rac1 activity and efficient bacterial internalization
    Sei Yoshida
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    EMBO J 21:2923-35. 2002
    ..These results indicate that VirA is a novel type of bacterial effector capable of inducing membrane ruffling through the stimulation of MT destabilization...
  23. ncbi request reprint A bacterial effector targets Mad2L2, an APC inhibitor, to modulate host cell cycling
    Hiroki Iwai
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell 130:611-23. 2007
    ..These results strongly indicate that Shigella employ special tactics to influence epithelial renewal in order to promote bacterial colonization of intestinal epithelium...
  24. ncbi request reprint Shigella chromosomal IpaH proteins are secreted via the type III secretion system and act as effectors
    Hiroshi Ashida
    Department of Microbiology and Immunology, International Research Center for Infectious Disease, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Tokyo 108 8639, Japan
    Mol Microbiol 63:680-93. 2007
    ....
  25. doi request reprint Shigella deploy multiple countermeasures against host innate immune responses
    Hiroshi Ashida
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Curr Opin Microbiol 14:16-23. 2011
    ..In this review we highlight current topics concerning Shigella's tactics that interfere with the innate immune systems...
  26. ncbi request reprint Grb2 is a key mediator of helicobacter pylori CagA protein activities
    Hitomi Mimuro
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Mol Cell 10:745-55. 2002
    ..pylori-induced gastric hyperplasia...
  27. ncbi request reprint Escape of intracellular Shigella from autophagy
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Science 307:727-31. 2005
    ..Rather, Shigella VirG, a protein required for intracellular actin-based motility, induced autophagy by binding to the autophagy protein, Atg5. In nonmutant Shigella, this binding is competitively inhibited by IcsB binding to VirG...
  28. ncbi request reprint Intracellular survival of Shigella
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell Microbiol 8:177-84. 2006
    ..In this article, we review recent advances in understanding of Shigella strategies to infect epithelial cells, and then focus on recent studies of an intriguing bacterial survival strategy against autophagic degradation...
  29. doi request reprint Shigella targets epithelial tricellular junctions and uses a noncanonical clathrin-dependent endocytic pathway to spread between cells
    Makoto Fukumatsu
    Department of Infectious Disease Control, International Research Center for Infectious Disease, University of Tokyo, Shirokanedai, Minato ku, Tokyo, Japan
    Cell Host Microbe 11:325-36. 2012
    ..Our findings highlight a mechanism for Shigella dissemination into neighboring cells via targeting of tricellular junctions and a noncanonical clathrin-dependent endocytic pathway...
  30. ncbi request reprint The versatility of Shigella effectors
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Rev Microbiol 6:11-6. 2008
    ..Although bacterial strategies and mechanisms of infection vary greatly, recent studies of Shigella effectors have revealed that Shigella possess a highly evolved strategy for infection...
  31. doi request reprint Bacterial interactions with the host epithelium
    Minsoo Kim
    Department of Infectious Disease Control, International Research Center for Infectious Diseases, University of Tokyo 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell Host Microbe 8:20-35. 2010
    ..These complex strategies represent a new paradigm of bacterial pathogenesis...
  32. ncbi request reprint Autophagy targeting of Listeria monocytogenes and the bacterial countermeasure
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Autophagy 7:310-4. 2011
    ..Here, we briefly review current knowledge of host recognition of L. monocytogenes by the innate immune system, and highlight how autophagic recognition by the host is overcome by bacterial countermeasures...
  33. pmc Shigella IpaH0722 E3 ubiquitin ligase effector targets TRAF2 to inhibit PKC-NF-κB activity in invaded epithelial cells
    Hiroshi Ashida
    Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato ku, Tokyo, Japan
    PLoS Pathog 9:e1003409. 2013
    ..IpaH0722 dampens the acute inflammatory response by preferentially inhibiting the PKC-mediated activation of NF-κB by ubiquitinating TRAF2, a molecule downstream of PKC, and by promoting its proteasome-dependent degradation...
  34. doi request reprint The bacterial effector Cif interferes with SCF ubiquitin ligase function by inhibiting deneddylation of Cullin1
    Hanako Morikawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 401:268-74. 2010
    ..We thus conclude that the bacterial effector Cif interferes with neddylation-mediated cell-cycle control...
  35. ncbi request reprint Listeria monocytogenes ActA is a key player in evading autophagic recognition
    Yuko Yoshikawa
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Autophagy 5:1220-1. 2009
    ..We show that L. monocytogenes exploits the biomimetic property of ActA to camouflage itself with host proteins comprised of Ena/VASP and the Arp2/3 complex, thereby escaping recognition by autophagy (Fig. 1)...
  36. ncbi request reprint Shigella effector IpaH9.8 binds to a splicing factor U2AF(35) to modulate host immune responses
    Jun Okuda
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8039, Japan
    Biochem Biophys Res Commun 333:531-9. 2005
    ..The results indicate that IpaH9.8 plays a role in Shigella infection to optimize the host inflammatory responses, thus facilitating bacterial colonization within the host epithelial cells...
  37. ncbi request reprint A novel in vitro infection model of Helicobacter pylori using mucin-producing murine gastric surface mucous cells
    Tetsufumi Takahashi
    Graduate School of Pharmaceutical Sciences, Kitasato University, Minato ku, Tokyo, Japan
    Helicobacter 9:302-12. 2004
    ..pylori infection in vitro, although cancer cells have some abnormalities in cellular properties. The aim of this study was to develop an in vitro H. pylori infection model using normal gastric surface cells that produce gastric mucin...
  38. pmc Enteropathogenic Escherichia coli activates the RhoA signaling pathway via the stimulation of GEF-H1
    Takeshi Matsuzawa
    Department of Infection Control and Immunology, Laboratory of Bacterial Infection, Kitasato Institute for Life Sciences, Kitasato University, Shirokane, Minato ku, Tokyo, Japan
    EMBO J 23:3570-82. 2004
    ..This report reveals for the first time that a pathogen can exploit the host factor GEF-H1...
  39. ncbi request reprint Exploiting host microtubule dynamics: a new aspect of bacterial invasion
    Sei Yoshida
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Trends Microbiol 11:139-43. 2003
    ....
  40. pmc Bicarbonate ion stimulates the expression of locus of enterocyte effacement-encoded genes in enterohemorrhagic Escherichia coli O157:H7
    Hiroyuki Abe
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Infect Immun 70:3500-9. 2002
    ....
  41. ncbi request reprint IpgB1 is a novel Shigella effector protein involved in bacterial invasion of host cells. Its activity to promote membrane ruffling via Rac1 and Cdc42 activation
    Kenji Ohya
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 280:24022-34. 2005
    ..The results thus indicate that IpgB1 is a novel Shigella effector involved in bacterial invasion of epithelial cells via the activation of Rho GTPases...
  42. doi request reprint Bacteria and host interactions in the gut epithelial barrier
    Hiroshi Ashida
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Nat Chem Biol 8:36-45. 2012
    ..We provide an overview of the components of the mucosal barrier and discuss the bacterial stratagems that circumvent these barriers with particular emphasis on the roles of bacterial effector proteins...
  43. pmc Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis
    Takashi Obata
    Division of Mucosal Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 107:7419-24. 2010
    ..Thus, indigenous opportunistic bacteria uniquely inhabit PPs, leading to PP-DCs-initiated, local antigen-specific Ab production; this may involve the creation of an optimal symbiotic environment on the interior of the PPs...
  44. ncbi request reprint Shigella Spa33 is an essential C-ring component of type III secretion machinery
    Tomoko Morita-Ishihara
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, International Research Center for Infectious Diseases, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 281:599-607. 2006
    ..Thus, Spa33 plays a central role as the C-ring component in recruiting/exporting TTSM-associated proteins...
  45. doi request reprint Shigella infection of intestinal epithelium and circumvention of the host innate defense system
    Hiroshi Ashida
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, Japan
    Curr Top Microbiol Immunol 337:231-55. 2009
    ..These examples suggest that Shigella have evolved highly sophisticated infectious and intracellular strategies to establish replicative niches in the intestinal epithelium...
  46. doi request reprint Manipulation of autophagy by bacteria for their own benefit
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Microbiol Immunol 55:459-71. 2011
    ..Also highlighted are some selective examples of bacterial activities that manipulate the autophagic pathways for their own benefit...
  47. ncbi request reprint Shigella IpgB1 promotes bacterial entry through the ELMO-Dock180 machinery
    Yutaka Handa
    Department of Microbiology and Immunology, Institute of Medical Science and University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Cell Biol 9:121-8. 2007
    ..Taken together, our findings show that IpgB1 mimicry is the key to invasion by Shigella...
  48. pmc Innate lymphoid cells regulate intestinal epithelial cell glycosylation
    Yoshiyuki Goto
    Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332 0012, Japan Microbe Division Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba 305 0074, Japan
    Science 345:1254009. 2014
    ..Disruption of intestinal fucosylation led to increased susceptibility to infection by Salmonella typhimurium. Our data reveal a role for ILC3 in shaping the gut microenvironment through the regulation of epithelial glycosylation. ..
  49. doi request reprint Streptococcus-, Shigella-, and Listeria-induced autophagy
    Michinaga Ogawa
    Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Methods Enzymol 452:363-81. 2009
    ....
  50. ncbi request reprint Purification and detection of Shigella type III secretion needle complex
    Koichi Tamano
    Department of Microbiology and Immunology, Division of Bacterial Infection, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Methods Enzymol 358:385-92. 2002
  51. pmc Role of Peyer's patches in the induction of Helicobacter pylori-induced gastritis
    Shigenori Nagai
    Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Proc Natl Acad Sci U S A 104:8971-6. 2007
    ..We also observed that the coccoid form of H. pylori is phagocytosed by dendritic cells in PPs. We propose that H. pylori converts to the coccoid form in the anaerobic small intestine and stimulates the host immune system through PPs...
  52. doi request reprint Exploitation of the host ubiquitin system by human bacterial pathogens
    Hiroshi Ashida
    Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Rev Microbiol 12:399-413. 2014
    ..These strategies enable bacterial pathogens to maximize effector function and obtain nutrients, thereby promoting bacterial proliferation. ..
  53. ncbi request reprint Bacterial evasion of the autophagic defense system
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Curr Opin Microbiol 9:62-8. 2006
    ..Indeed, recent reports describe intracellular pathogens as being capable of subverting or modifying autophagy activation and persisting within autophagosomes...
  54. pmc Bacterial effectors and their functions in the ubiquitin-proteasome system: insight from the modes of substrate recognition
    Minsoo Kim
    Division of Bacterial Infection Biology, Institute of Medical Science, The University of Tokyo, Shirokanedai 4 6 1, Minato ku 4 6 1, Tokyo 108 8639, Japan
    Cells 3:848-64. 2014
    ..This review will describe recent findings, based on structural analyses, regarding how pathogens use post-translational modifications of proteins to establish an infection. ..
  55. pmc Shigella type III secretion protein MxiI is recognized by Naip2 to induce Nlrc4 inflammasome activation independently of Pkcδ
    Shiho Suzuki
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan, United States of America Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    PLoS Pathog 10:e1003926. 2014
    ..These results indicate that activation of caspase-1 by Shigella is triggered by the rod protein MxiI that interacts with Naip2 to induce activation of the Nlrc4 inflammasome independently of the Pkcδ kinase. ..
  56. doi request reprint The role of Tecpr1 in selective autophagy as a cargo receptor
    Michinaga Ogawa
    Division of Bacterial Infection, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Autophagy 7:1389-91. 2011
    ..Tecpr1 plays an important role in promoting selective autophagy via the WIPI-2-Tecpr1-Atg5 pathway in targeting bacteria, protein aggregates and damaged mitochondria...
  57. ncbi request reprint Microtubule-severing activity of Shigella is pivotal for intercellular spreading
    Sei Yoshida
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Science 314:985-9. 2006
    ..Degradation of microtubules by VirA was dependent on its alpha-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells...
  58. doi request reprint A rice-based oral cholera vaccine induces macaque-specific systemic neutralizing antibodies but does not influence pre-existing intestinal immunity
    Tomonori Nochi
    Division of Mucosal Immunology, Department of Microbiology and Immunology, University of Tokyo, Japan
    J Immunol 183:6538-44. 2009
    ..These results show that oral MucoRice-CT-B can effectively induce CT-specific, neutralizing, serum IgG Ab responses even in the presence of pre-existing CT- and heat-labile enterotoxin-reactive intestinal SIgA Abs in nonhuman primates...
  59. doi request reprint Manipulation of the host cell death pathway by Shigella
    Hiroshi Ashida
    Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo, Tokyo, 108 8639, Japan
    Cell Microbiol 16:1757-66. 2014
    ..However, many bacterial pathogens, including Shigella, have evolved mechanisms that manipulate host cell death for their own benefit...
  60. ncbi request reprint High vaccine efficacy against shigellosis of recombinant noninvasive Shigella mutant that expresses Yersinia invasin
    Toshihiko Suzuki
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Immunol 177:4709-17. 2006
    ....
  61. ncbi request reprint A novel caspase-1/toll-like receptor 4-independent pathway of cell death induced by cytosolic Shigella in infected macrophages
    Toshihiko Suzuki
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 280:14042-50. 2005
    ..These results identify a novel cell death pathway induced by intracellular Gram-negative bacteria that may play a role in microbial-host interactions and inflammatory responses...
  62. pmc Shigella Spa32 is an essential secretory protein for functional type III secretion machinery and uniformity of its needle length
    Koichi Tamano
    Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639
    J Bacteriol 184:1244-52. 2002
    ..and is involved in the control of needle length. Furthermore, its function is interchangeable with that of Salmonella InvJ...
  63. doi request reprint Midori-ishi Cyan/monomeric Kusabira-Orange-based fluorescence resonance energy transfer assay for characterization of various E3 ligases
    Ryota Otsubo
    Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo, Japan
    Genes Cells 21:608-23. 2016
    ..Based on these results, we concluded that the MiCy/mKO-based FRET assay is useful for characterizing E3 enzyme activity, as well as for high-throughput E3 inhibitor screening. ..
  64. pmc Shigella IpaH Family Effectors as a Versatile Model for Studying Pathogenic Bacteria
    Hiroshi Ashida
    Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo Tokyo, Japan
    Front Cell Infect Microbiol 5:100. 2015
    ....
  65. pmc Shigella hacks host immune responses by reprogramming the host epigenome
    Hiroshi Ashida
    Division of Bacterial Infection Biology, Institute of Medical Science, University of Tokyo, Minato ku Tokyo, Japan
    EMBO J 33:2598-600. 2014
    ..These results illustrate how bacterial pathogens exploit epigenetic modifications to counteract host immune responses...
  66. doi request reprint Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses
    Harumichi Ishigame
    Center for Experimental Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Immunity 30:108-19. 2009
    ..These observations indicate that IL-17A and IL-17F have overlapping yet distinct roles in host immune and defense mechanisms...
  67. ncbi request reprint Shigella and autophagy
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Sceince, University of Tokyo, Tokyo, Japan
    Autophagy 2:171-4. 2006
    ..In this review we discuss recent discoveries regarding Shigella strategies for infecting mammalian cells, and then focus on recent studies of an elegant bacterial survival strategy against autophagic degradation...
  68. pmc Id2-, RORgammat-, and LTbetaR-independent initiation of lymphoid organogenesis in ocular immunity
    Takahiro Nagatake
    Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    J Exp Med 206:2351-64. 2009
    ..Thus, TALT shares immunological features with MALT but has a distinct tissue genesis mechanism and plays a key role in ocular immunity...
  69. ncbi request reprint Species-specific cell adhesion of enteropathogenic Escherichia coli is mediated by type IV bundle-forming pili
    Toru Tobe
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minatoku, Tokyo 108 8639, Japan
    Cell Microbiol 4:29-42. 2002
    ..These results indicated that BFP plays an important role in the cell-type-dependent adherence of EPEC and in the progression to the later steps in EPEC adherence...