Kenji Sugase

Summary

Affiliation: Suntory Institute for Bioorganic Research
Country: Japan

Publications

  1. ncbi Designing analogues of mini atrial natriuretic peptide based on structural analysis by NMR and restrained molecular dynamics
    Kenji Sugase
    Suntory Institute for Bioorganic Research and Suntory Institute for Biomedical Research, Shimamoto, Mishima 618 8503, Japan
    J Med Chem 45:881-7. 2002
  2. ncbi Mechanism of coupled folding and binding of an intrinsically disordered protein
    Kenji Sugase
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 447:1021-5. 2007
  3. pmc Overexpression of post-translationally modified peptides in Escherichia coli by co-expression with modifying enzymes
    Kenji Sugase
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Protein Expr Purif 57:108-15. 2008
  4. ncbi [Intrinsically disordered protein and encounter complex]
    Kenji Sugase
    Tanpakushitsu Kakusan Koso 52:945-51. 2007
  5. doi Elucidating slow binding kinetics of a protein without observable bound resonances by longitudinal relaxation NMR spectroscopy
    Kenji Sugase
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, Wakayamadai, Shimamoto cho, Mishima gun, Osaka, Japan
    J Biomol NMR 50:219-27. 2011
  6. doi Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1
    Erisa Harada
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka, 618 8503, Japan
    Biomol NMR Assign 9:197-200. 2015
  7. ncbi Solution structure of IsTX. A male scorpion toxin from Opisthacanthus madagascariensis (Ischnuridae)
    Nahoko Yamaji
    Suntory Institute for Bioorganic Research, Mishima gun, Osaka 618 8503, Japan
    Eur J Biochem 271:3855-64. 2004
  8. pmc The helical propensity of the extracellular loop is responsible for the substrate specificity of Fe(III)-phytosiderophore transporters
    Erisa Harada
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, Japan
    FEBS Lett . 2016
  9. doi Use of glass capillaries to suppress thermal convection in NMR tubes in diffusion measurements
    Takashi Iwashita
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8 1 1 Seikadai, Seika cho, Soraku gun, Kyoto, 619 0284, Japan
    Magn Reson Chem . 2016
  10. ncbi [Applications of relaxation dispersion spectroscopy]
    Kenji Sugase
    Division of Spectroscopic and Structural Research, Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto cho, Mishima gun, Osaka 618 8503, Japan
    Seikagaku 80:754-8. 2008

Collaborators

Detail Information

Publications21

  1. ncbi Designing analogues of mini atrial natriuretic peptide based on structural analysis by NMR and restrained molecular dynamics
    Kenji Sugase
    Suntory Institute for Bioorganic Research and Suntory Institute for Biomedical Research, Shimamoto, Mishima 618 8503, Japan
    J Med Chem 45:881-7. 2002
    ..Conformational analysis of these analogue peptides by NMR suggested that a turnlike conformation at residues 6-9 and a proximate pair formed by side chains of Phe(4) and Ile(11) are important for the biological activity...
  2. ncbi Mechanism of coupled folding and binding of an intrinsically disordered protein
    Kenji Sugase
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 447:1021-5. 2007
    ..Future applications of our method will provide new understanding of the molecular mechanisms by which intrinsically disordered proteins perform their diverse biological functions...
  3. pmc Overexpression of post-translationally modified peptides in Escherichia coli by co-expression with modifying enzymes
    Kenji Sugase
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Protein Expr Purif 57:108-15. 2008
    ..Our GB1-fusion co-expression system can be used in the same way as conventional protein expression in E. coli, making it a flexible and economical method to produce a large amount of a post-translationally modified protein...
  4. ncbi [Intrinsically disordered protein and encounter complex]
    Kenji Sugase
    Tanpakushitsu Kakusan Koso 52:945-51. 2007
  5. doi Elucidating slow binding kinetics of a protein without observable bound resonances by longitudinal relaxation NMR spectroscopy
    Kenji Sugase
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, Wakayamadai, Shimamoto cho, Mishima gun, Osaka, Japan
    J Biomol NMR 50:219-27. 2011
    ..We applied the method to binding of the B1 domain of protein G (GB1) to immunoglobulin G, and derived the binding kinetics despite the absence of observable bound GB1 resonances...
  6. doi Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1
    Erisa Harada
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka, 618 8503, Japan
    Biomol NMR Assign 9:197-200. 2015
    ..ZnPP is a potent enzyme inhibitor, and the ZnPP-bound structure of HO-1 mimics the heme-bound structure. These assignments provide the structural basis for a detailed investigation of the HO-1 function. ..
  7. ncbi Solution structure of IsTX. A male scorpion toxin from Opisthacanthus madagascariensis (Ischnuridae)
    Nahoko Yamaji
    Suntory Institute for Bioorganic Research, Mishima gun, Osaka 618 8503, Japan
    Eur J Biochem 271:3855-64. 2004
    ..MALDI-TOF MS analysis clearly indicated that IsTX was found in male scorpion venom, but not in female. This is the first report that scorpion venom contains sex-specific compounds...
  8. pmc The helical propensity of the extracellular loop is responsible for the substrate specificity of Fe(III)-phytosiderophore transporters
    Erisa Harada
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, Japan
    FEBS Lett . 2016
    ..These structural differences are potentially responsible for the substrate specificities of the transporters...
  9. doi Use of glass capillaries to suppress thermal convection in NMR tubes in diffusion measurements
    Takashi Iwashita
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8 1 1 Seikadai, Seika cho, Soraku gun, Kyoto, 619 0284, Japan
    Magn Reson Chem . 2016
    ..Capillaries are a simple and inexpensive means of suppressing thermal convection and thus can be used in a wide variety of DOSY experiments. Copyright © 2016 John Wiley & Sons, Ltd...
  10. ncbi [Applications of relaxation dispersion spectroscopy]
    Kenji Sugase
    Division of Spectroscopic and Structural Research, Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto cho, Mishima gun, Osaka 618 8503, Japan
    Seikagaku 80:754-8. 2008
  11. doi Extracting protein dynamics information from overlapped NMR signals using relaxation dispersion difference NMR spectroscopy
    Tsuyoshi Konuma
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka, 618 8503, Japan
    J Biomol NMR 63:367-73. 2015
    ..Relaxation dispersion difference requires neither selectively labeled samples nor modification of pulse programs; thus it will have wide applications in protein dynamics analysis. ..
  12. doi Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system
    Kaoru Nomura
    Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto cho, Mishima gun, Osaka, Japan
    Innate Immun 17:256-68. 2011
    ..Thus, this study shows the possibility that LPS induces the expansion of raft region and the rapid motion of the raft-forming membranes to favor molecular interactions in the animal cell membrane during innate immune recognition...
  13. ncbi Calcitonin in a protochordate, Ciona intestinalis--the prototype of the vertebrate calcitonin/calcitonin gene-related peptide superfamily
    Toshio Sekiguchi
    Suntory Institute for Bioorganic Research, Osaka, Japan
    FEBS J 276:4437-47. 2009
    ..This is the first report on a CT/CGRP superfamily member in invertebrates...
  14. ncbi Solution structure of agelenin, an insecticidal peptide isolated from the spider Agelena opulenta, and its structural similarities to insect-specific calcium channel inhibitors
    Nahoko Yamaji
    Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto cho, Mishima gun, Osaka 618 8503, Japan
    FEBS Lett 581:3789-94. 2007
    ..We also suggest that another mode of action may operate in the channel inhibition by omega-agatoxin-IVA and omega-atracotoxin-Hv2a...
  15. ncbi Structural element responsible for the Fe(III)-phytosiderophore specific transport by HvYS1 transporter in barley
    Emiko Harada
    Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto cho, Mishima gun, Osaka 618 8503, Japan
    FEBS Lett 581:4298-302. 2007
    ..We propose that the structural difference at this particular loop determines the substrate specificity of the HvYS1 transporter...
  16. doi Quantitative analysis of protein-ligand interactions by NMR
    Ayako Furukawa
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka 618 8503, Japan Graduate School of Medical Life Science, Yokohama City University, 1 7 29 Suehiro cho, Tsurumi ku, Yokohama 230 0045, Japan
    Prog Nucl Magn Reson Spectrosc 96:47-57. 2016
    ..In this review, we first explain the exchange regimes and kinetic models of protein-ligand interactions, and then describe the NMR methods that quantitatively analyze these specific interactions. ..
  17. doi Distal regulation of heme binding of heme oxygenase-1 mediated by conformational fluctuations
    Erisa Harada
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka 618 8503, Japan
    Biochemistry 54:340-8. 2015
    ..Similar to other enzymes, HO-1 effectively promotes its function using the identified distal sites, which might be potential targets for protein engineering. ..
  18. doi Solid-state NMR spectra of lipid-anchored proteins under magic angle spinning
    Kaoru Nomura
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai, Shimamoto cho, Mishima gun, Osaka 618 8503, Japan
    J Phys Chem B 118:2405-13. 2014
    ..These results demonstrated the applicability of two types of sample system to elucidate the roles of lipid-anchors in regulating diverse biological phenomena. ..
  19. pmc Fast and accurate fitting of relaxation dispersion data using the flexible software package GLOVE
    Kenji Sugase
    Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1 1 1 Wakayamadai Shimamoto, Mishima, Osaka, 618 8503, Japan
    J Biomol NMR 56:275-83. 2013
    ..We demonstrate here that the combined use of the three methods can find the global minimum more rapidly and more accurately than grid search alone. ..
  20. ncbi Restriction of a peptide turn conformation and conformational analysis of guanidino group using arginine-proline fused amino acids: application to mini atrial natriuretic peptide on binding to the receptor
    Kenji Sugase
    Suntory Institute for Bioorganic Research and Daiichi Suntory Biomedical Research Co, Ltd, Shimamoto, Mishima 618 8503, Japan
    J Med Chem 47:489-92. 2004
    ..Structural analysis revealed that the size of the conformational space of Arg(7) on binding to the receptor was approximately one-third of the entire conformational space...
  21. pmc Tailoring relaxation dispersion experiments for fast-associating protein complexes
    Kenji Sugase
    Department of Molecular Biology and Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Am Chem Soc 129:13406-7. 2007