Yasuko Nakano

Summary

Affiliation: Showa University
Country: Japan

Publications

  1. ncbi request reprint A novel enzyme-linked immunosorbent assay specific for high-molecular-weight adiponectin
    Yasuko Nakano
    Department of Medicinal Information, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Tokyo 142 8555, Japan
    J Lipid Res 47:1572-82. 2006
  2. pmc Adiponectin does not bind to gelatin: a new and easy way to purify high-molecular-weight adiponectin from human plasma
    Yasuko Nakano
    Department of Pharmacogenomics School of Pharmacy, Showa University, 1 5 8, Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Lipid Res 51:210-5. 2010
  3. ncbi request reprint Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma
    Y Nakano
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    J Biochem 120:803-12. 1996
  4. doi request reprint Potentiated macrophage activation by acid sensing under low adiponectin levels
    Takaharu Negoro
    Department of Pharmacogenomics, Showa University School of Pharmacy, Shinagawa ku, Tokyo 142 8555, Japan Electronic address
    Mol Immunol 57:141-50. 2014
  5. doi request reprint A simple smart amplification assay for the rapid detection of human cytomegalovirus in the urine of neonates
    Chikara Kohda
    Department of Microbiology and Immunology, Showa University, School of Medicine, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan Electronic address
    J Virol Methods 208:160-5. 2014
  6. doi request reprint Adiponectin enhances calcium dependency of mouse bladder contraction mediated by protein kinase Cα expression
    Koji Nobe
    Department of Pharmacology, School of Pharmacy, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Pharmacol Exp Ther 345:62-8. 2013
  7. ncbi request reprint Characterization of mouse GBP28 and its induction by exposure to cold
    M Yoda
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Int J Obes Relat Metab Disord 25:75-83. 2001
  8. ncbi request reprint Organization of the gene for gelatin-binding protein (GBP28)
    K Saito
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Gene 229:67-73. 1999
  9. ncbi request reprint Adiponectin plays an important role in efficient energy usage under energy shortage
    Kiyomi Saito
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Shinagawa ku, Tokyo 142 8555, Japan
    Biochim Biophys Acta 1761:709-16. 2006
  10. doi request reprint Neutrophil TRPM2 channels are implicated in the exacerbation of myocardial ischaemia/reperfusion injury
    Toshihito Hiroi
    Department of Pathophysiology, Showa University School of Pharmacy, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    Cardiovasc Res 97:271-81. 2013

Collaborators

Detail Information

Publications50

  1. ncbi request reprint A novel enzyme-linked immunosorbent assay specific for high-molecular-weight adiponectin
    Yasuko Nakano
    Department of Medicinal Information, School of Pharmaceutical Sciences, Showa University, 1 5 8 Hatanodai, Tokyo 142 8555, Japan
    J Lipid Res 47:1572-82. 2006
    ..4 +/- 5.5 microg/ml (mean +/- SD) in healthy women and 6.2 +/- 3.6 microg/ml in healthy men. Also, serum with a lower HMW adiponectin concentration was shown to have a lower HMW ratio (i.e., HMW adiponectin/total adiponectin)...
  2. pmc Adiponectin does not bind to gelatin: a new and easy way to purify high-molecular-weight adiponectin from human plasma
    Yasuko Nakano
    Department of Pharmacogenomics School of Pharmacy, Showa University, 1 5 8, Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Lipid Res 51:210-5. 2010
    ..Through only two steps using reduced formyl-Cellulofine and DEAE-Sepharose, HMW adiponectin can be effectively purified from human plasma...
  3. ncbi request reprint Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma
    Y Nakano
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    J Biochem 120:803-12. 1996
    ..Although its biological function is presently unclear, its adipocyte-specific expression suggests that GBP28 may function as an endogenous factor involved in lipid catabolism and storage or whole body metabolism...
  4. doi request reprint Potentiated macrophage activation by acid sensing under low adiponectin levels
    Takaharu Negoro
    Department of Pharmacogenomics, Showa University School of Pharmacy, Shinagawa ku, Tokyo 142 8555, Japan Electronic address
    Mol Immunol 57:141-50. 2014
    ..Our results indicated that in AsTg mice, the Ca(2)(+) response to the acid was facilitated in MΦ by a low threshold of ASIC1 and NCX1 molecules and the activity of these channel was possibly regulated by adiponectin...
  5. doi request reprint A simple smart amplification assay for the rapid detection of human cytomegalovirus in the urine of neonates
    Chikara Kohda
    Department of Microbiology and Immunology, Showa University, School of Medicine, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan Electronic address
    J Virol Methods 208:160-5. 2014
    ..In brief, this SMAP-based assay provides a simple, rapid and efficient method for detecting human CMV at the bedside...
  6. doi request reprint Adiponectin enhances calcium dependency of mouse bladder contraction mediated by protein kinase Cα expression
    Koji Nobe
    Department of Pharmacology, School of Pharmacy, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Pharmacol Exp Ther 345:62-8. 2013
    ..In conclusion, adiponectin is associated with bladder smooth muscle contraction, which involves an increase in Ca(2+) dependency of contraction mediated by PKCα expression...
  7. ncbi request reprint Characterization of mouse GBP28 and its induction by exposure to cold
    M Yoda
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Int J Obes Relat Metab Disord 25:75-83. 2001
    ..CONCLUSION: GBP28 may play a role in homeostasis, regulating body temperature and basal metabolic rate in response to changing environmental conditions. International Journal of Obesity (2001) 25, 75-83..
  8. ncbi request reprint Organization of the gene for gelatin-binding protein (GBP28)
    K Saito
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Gene 229:67-73. 1999
    ..The GBP28 gene was located on human chromosome 3q27. The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the accession numbers ABO12163, ABO12164 or ABO12165...
  9. ncbi request reprint Adiponectin plays an important role in efficient energy usage under energy shortage
    Kiyomi Saito
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Shinagawa ku, Tokyo 142 8555, Japan
    Biochim Biophys Acta 1761:709-16. 2006
    ..These findings suggest that the serum adiponectin is important to maintaining energy homeostasis under energy shortage conditions, such as over female pubertal development...
  10. doi request reprint Neutrophil TRPM2 channels are implicated in the exacerbation of myocardial ischaemia/reperfusion injury
    Toshihito Hiroi
    Department of Pathophysiology, Showa University School of Pharmacy, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    Cardiovasc Res 97:271-81. 2013
    ..Myocardial ischaemia/reperfusion (I/R) injury is characterized by acute inflammation associated with the augmentation of oxidative stress. We hypothesized that TRPM2 is implicated in the exacerbation of myocardial I/R injury...
  11. doi request reprint Low HCMV DNA copies can establish infection and result in significant symptoms in extremely preterm infants: a prospective study
    Hitomi Wakabayashi
    Department of Pharmacogenomics, Showa University School of Medicine, Shinagawa ku, Tokyo, Japan
    Am J Perinatol 29:377-82. 2012
    ..Symptomatic infants had longer stays and slower weight gain. The HCMV infection rate is high in very preterm infants. A new strategy to prevent HCMV infection other than freezing should therefore be established...
  12. doi request reprint Analysis of epitope regions for autoantibodies in catalase
    Hiromi Miura
    Department of Pharmacogenomics, School of Pharmaceutical Science, Showa University, Tokyo, Japan
    Immunol Invest 39:796-806. 2010
    ..In conclusion, although AE-1 and -2 of catalase have been reported to contribute to autoimmune responses in animals infected with catalase-producing pathogens, AE-3 and -4 appear to have a different mechanism for autoantibody production...
  13. ncbi request reprint Isolation of two forms of decay-accelerating factor (DAF) from human urine
    Y Nakano
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Biochim Biophys Acta 1074:326-30. 1991
    ..However, both urine DAFs revealed a similar activity to each other and slightly higher activity than that of membrane form DAF in decay-accelerating fluid-phase C3 convertase of the alternative pathway...
  14. ncbi request reprint Purification and characterization of a novel glycoprotein which has significant homology to heavy chains of inter-alpha-trypsin inhibitor family from human plasma
    N H Choi-Miura
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo
    J Biochem 117:400-7. 1995
    ..We designated GP120 as IHRP (ITI heavy chain-related protein)...
  15. ncbi request reprint Purification and characterization of a novel hyaluronan-binding protein (PHBP) from human plasma: it has three EGF, a kringle and a serine protease domain, similar to hepatocyte growth factor activator
    N H Choi-Miura
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo
    J Biochem 119:1157-65. 1996
    ....
  16. ncbi request reprint Isolation and characterization of the human inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) gene (ITIHL1)
    K Saguchi
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo
    J Biochem 119:898-905. 1996
    ..This gene was found to be localized very close to another unknown gene related to EST (GenBank accession #: R54643, R50663, R50563, H27139, and R54913)...
  17. ncbi request reprint Determination of carboxyl-terminal residue and disulfide bonds of MACIF (CD59), a glycosyl-phosphatidylinositol-anchored membrane protein
    Y Sugita
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo
    J Biochem 114:473-7. 1993
    ..The results indicated that disulfide bonds were formed at Cys3-Cys26, Cys6-Cys13, Cys19-Cys39, Cys45-Cys63 (or 64), and Cys63 (or 64)-Cys69...
  18. ncbi request reprint Cloning and characterization of cDNA for inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP), a novel human plasma glycoprotein
    K Saguchi
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo
    J Biochem 117:14-8. 1995
    ..The cleaved site, Arg-Arg-Leu, was within a proline-rich region. Northern blot analysis of poly(A) RNAs from various human tissues only showed hybridization to liver RNA...
  19. ncbi request reprint Complete determination of disulfide bonds localized within the short consensus repeat units of decay accelerating factor (CD55 antigen)
    Y Nakano
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Biochim Biophys Acta 1116:235-40. 1992
    ..From sequence analyses of these peptides, we could identify all disulfide bonds in the 4 SCR units of DAF as being between the first and the third and between the second and the fourth half-cystines within each SCR unit...
  20. ncbi request reprint NADPH-dependent glutathione peroxidase-like proteins (Gpx-1, Gpx-2) reduce unsaturated fatty acid hydroperoxides in Synechocystis PCC 6803
    A Gaber
    Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Japan
    FEBS Lett 499:32-6. 2001
    ..It seems accurate to refer to Gpx-1 and Gpx-2 as NADPH-dependent GPX-like proteins that serve as a new defense system for the reduction of unsaturated fatty acid hydroperoxides...
  21. ncbi request reprint Characterization of monoclonal antibodies against ascorbate peroxidase isoenzymes: purification and epitope-mapping using immunoaffinity column chromatography
    K Yoshimura
    Department of Food and Nutrition, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan
    Biochim Biophys Acta 1526:168-74. 2001
    ..We concluded that the peptides identified as epitopes are characteristic evidence of monoclonal antibodies...
  22. ncbi request reprint The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity
    T Yamauchi
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Nat Med 7:941-6. 2001
    ..These data also indicate that the replenishment of adiponectin might provide a novel treatment modality for insulin resistance and type 2 diabetes...
  23. ncbi request reprint Isolation and characterization of the rml gene homologs from Porphyromonas gingivalis
    Y Shibata
    Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan
    Oral Microbiol Immunol 14:339-47. 1999
    ..These results suggest that the rml gene homologs might be essential for the viability of P. gingivalis under the culture conditions used in this study...
  24. ncbi request reprint Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig
    K Karasawa
    Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa
    J Biochem 120:838-44. 1996
    ....
  25. ncbi request reprint Functional domains and sub-cellular distribution of the Hedgehog transducing protein Smoothened in Drosophila
    Y Nakano
    MRC Intercellular Signalling Group, Centre for Developmental Genetics, University School of Medicine and Biomedical Science, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Mech Dev 121:507-18. 2004
    ..We further demonstrate that Smo accumulation is likely to be a cause, rather than a consequence, of Hh signal transduction...
  26. ncbi request reprint A searchable database for proteomes of oral microorganisms
    Y Nakano
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka, Japan
    Oral Microbiol Immunol 20:344-8. 2005
    ..mutans, demonstrating the usefulness of the system. This database is available at http://www.myamagu.dent.kyushu-u.ac.jp/~bioinformatics/index.html or http://www.bipos.mascat.nihon-u.ac.jp/index.html...
  27. ncbi request reprint Molecular cloning and expression of human liver biliverdin-IX beta reductase
    A Komuro
    Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    Biol Pharm Bull 19:796-804. 1996
    ..The recombinant and native biliverdin-IX beta reductases were indistinguishable as far as their mobility on SDS-PAGE gel, immunoreactivity and specific activity were concerned...
  28. ncbi request reprint Pantothenate synthetase from Escherichia coli [D-pantoate: beta-alanine ligase (AMP-forming), EC 6.3.2.1]
    K Miyatake
    Methods Enzymol 62:215-9. 1979
  29. ncbi request reprint Improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition
    T Takeshita
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Higashi ku, Fukuoka, Japan
    Oral Microbiol Immunol 22:419-28. 2007
    ..To minimize sizing errors, we proposed optimizing the size definition of internal standards...
  30. ncbi request reprint A protein with several possible membrane-spanning domains encoded by the Drosophila segment polarity gene patched
    Y Nakano
    ICRF Developmental Biology Unit, Department of Zoology, Oxford, UK
    Nature 341:508-13. 1989
    ..Here we report the molecular characterization of one such gene, patched. We find that patched encodes a large protein with several possible membrane-spanning domains and is expressed in a complex pattern during embryogenesis...
  31. ncbi request reprint Contrasting distributions of patched and hedgehog proteins in the Drosophila embryo
    A M Taylor
    ICRF Developmental Biology Unit, Department of Zoology, Oxford, UK
    Mech Dev 42:89-96. 1993
    ..The results are consistent with the hh protein being secreted by cells in which it is expressed and support a role for ptc in the reception of the putative hh encoded signal...
  32. ncbi request reprint Solution structure of a human cystatin A variant, cystatin A2-98 M65L, by NMR spectroscopy. A possible role of the interactions between the N- and C-termini to maintain the inhibitory active form of cystatin A
    S Tate
    Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan
    Biochemistry 34:14637-48. 1995
    ..abstract truncated at 400 words)..
  33. ncbi request reprint Purification and characterization of a repressor for the Bacillus cereus glnRA operon
    Y Nakano
    Laboratory of Biochemistry, College of Science, Rikkyo St Paul s University, Tokyo
    J Biochem 109:223-8. 1991
    ..These results indicate the existence of an interaction between GlnR and glutamine synthetase, and support the hypothesis that the regulation of glnA expression requires both GlnR protein and glutamine synthetase in Bacillus...
  34. ncbi request reprint Role of the Drosophila patched gene in positional signalling
    P W Ingham
    ICRF Developmental Biology Unit, Department of Zoology, Oxford, UK
    Nature 353:184-7. 1991
    ..We suggest that the patched protein may itself be the receptor for this signal, implying that this is an unusual mechanism of ligand-dependent receptor inactivation...
  35. ncbi request reprint Pantothenate synthetase of Escherichia coli B. I. Physicochemical properties
    K Miyatake
    J Biochem 79:673-8. 1976
    ..71 cm3/g. The enzyme had an amino-terminal glycyl residue and a Leu-Ala-Ser-OH sequence at the carboxyl end. Electrophoresis of the enzyme with carrier ampholine gave an isoelectric point of pH 4.6...
  36. ncbi request reprint HSpin1, a transmembrane protein interacting with Bcl-2/Bcl-xL, induces a caspase-independent autophagic cell death
    H Yanagisawa
    School of Human Sciences and Advanced Research Institute for Science and Engineering, Waseda University, 2 579 15 Mikajima, Tokorozawa, Saitama 359 1192, Japan
    Cell Death Differ 10:798-807. 2003
    ..HSpin1-induced cell death increases autophagic vacuole and mature form of cathepsin D, suggesting a novel caspase-independent cell death, which is link to autophagy...
  37. ncbi request reprint Large-scale production and properties of human plasma-derived activated Factor VII concentrate
    K Tomokiyo
    Blood Products Research Department, The Chemo Sero Therapeutic Research Institute, Kaketsuken, Kumamoto, Japan
    Vox Sang 84:54-64. 2003
    ..In the present study, we attempted to establish a large-scale manufacturing process to obtain plasma-derived FVIIa concentrate with high recovery and safety, and to characterize its biochemical and biological properties...
  38. ncbi request reprint CD4(+) v(alpha)14 NKT cells play a crucial role in an early stage of protective immunity against infection with Leishmania major
    H Ishikawa
    Department of Parasitology and Immunology, University of Tokushima School of Medicine, 3 18 Kuramoto, Tokusima 770 8503, Japan
    Int Immunol 12:1267-74. 2000
    ..The NKT cell subset bore CD3, CD4, TCR alpha beta, IL-2R beta and NK1.1 but scarcely asialo-GM(1). Moreover, this effector subset was confirmed to be V(alpha)14 NKT cells by using J(alpha)281(-/-) mice...
  39. ncbi request reprint Patched represses the Hedgehog signalling pathway by promoting modification of the Smoothened protein
    P W Ingham
    MRC Intercellular Signalling Group, Centre for Developmental Genetics, University of Sheffield, UK
    Curr Biol 10:1315-8. 2000
    ..Our findings suggest that Smo is modified to yield a non-functional form and this modification is promoted by Ptc in a non-stoichiometric manner...
  40. ncbi request reprint Presenilin-1 mutations downregulate the signalling pathway of the unfolded-protein response
    T Katayama
    Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nat Cell Biol 1:479-85. 1999
    ..These results show that mutations in PS1 may increase vulnerability to ER stress by altering the UPR signalling pathway...
  41. ncbi request reprint Accumulation of murine amyloidbeta42 in a gene-dosage-dependent manner in PS1 'knock-in' mice
    Y Nakano
    Department of Environmental Medicine, Osaka University Medical School, Japan
    Eur J Neurosci 11:2577-81. 1999
    ....
  42. ncbi request reprint Activating mutation of D835 within the activation loop of FLT3 in human hematologic malignancies
    Y Yamamoto
    Department of Infectious Diseases and the First Department of Internal Medicine, Nagoya University School of Medicine, Japan
    Blood 97:2434-9. 2001
    ..These results demonstrate that the FLT3 gene is the target most frequently mutated to become constitutively active in AML...
  43. ncbi request reprint The intron 5-inserted form of rat erythropoietin receptor is expressed as a membrane-bound form
    R Yamaji
    Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka 599 8531, Japan
    Biochim Biophys Acta 1403:169-78. 1998
    ....
  44. ncbi request reprint Biological functions of UDP-glucose synthesis in Streptococcus mutans
    Y Yamashita
    Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan
    Microbiology 144:1235-45. 1998
    ..mutans (as a glucose donor for side-chain formation), but is also important for the organism's viability in environmental conditions of low pH...
  45. ncbi request reprint CR1 density polymorphism on erythrocytes of falciparum malaria patients in Thailand
    E Nagayasu
    The Institute of Science and Technology, Tokai University, Isehara, Japan
    Am J Trop Med Hyg 64:1-5. 2001
    ..0001) and heterozygotes (HL) (P = 0.013). The results suggest that a genetically-determined low CR1 density on erythrocytes may be a risk factor for developing a more severe form of malaria in Thai subjects...
  46. ncbi request reprint Effects of a novel hepatoprotective drug, ZNC-2381, on fas-induced hepatocellular caspase-3 activity and apoptosis in mice
    Y Segawa
    Central Research Laboratories, Zeria Pharmaceutical Co, Kohnan-machi, Ohsato-gun, Saitama, Japan
    Pharmacology 62:80-6. 2001
    ..We propose that the mechanism of action of ZNC-2381 may involve blockade of the signal transduction pathway (caspase-3) of apoptosis mediated by anti-Fas ab...
  47. ncbi request reprint CD4+ T cells are required for HSP65 expression in host macrophages and for protection of mice infected with Plasmodium yoelii
    M Zhang
    Department of Parasitology and Immunology, The University of Tokushima, School of Medicine, Tokushima, Japan
    Parasitol Int 50:201-9. 2001
    ..yoelii. In conclusion, HSP65 may play a role in preventing apoptosis of macrophages in mice infected with P. yoelii. CD4+ T cells are required for HSP65 expression and for protective immunity against P. yoelii infection...
  48. pmc Passive immunization with milk produced from an immunized cow prevents oral recolonization by Streptococcus mutans
    Y Shimazaki
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan
    Clin Diagn Lab Immunol 8:1136-9. 2001
    ..These results suggest that milk produced from immunized cow may be useful for controlling S. mutans in the human oral cavity...
  49. ncbi request reprint Phosphatase activities of rat intestinal enterocytes and their relation to diverse luminal pH, with special references to the possible localization of phytase along the brush border membrane
    Y Nakano
    Graduate School of Tokyo Medical and Dental University, Japan
    Arch Histol Cytol 64:483-92. 2001
    ....
  50. ncbi request reprint Alkaline and acid phosphatases in bone cells serve as phosphohydrolases at physiological pH in vivo: a histochemical implication
    Y Nakano
    Biostructural Science, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan
    Connect Tissue Res 44:219-22. 2003
    ..Combined specificity tests suggested that TNAP and ACP in bone cells can serve as phosphohydrolases at pH 7.3 and that reactions along the osteoidal surface of osteoblasts differ from that of TNAP and represent novel enzyme...