Hiroshi Watarai

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. pmc PDC-TREM, a plasmacytoid dendritic cell-specific receptor, is responsible for augmented production of type I interferon
    Hiroshi Watarai
    Laboratories for Immune Regulation and Host Defense, RIKEN Research Center for Allergy and Immunology, 1 7 22, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Proc Natl Acad Sci U S A 105:2993-8. 2008
  2. pmc Development and function of invariant natural killer T cells producing T(h)2- and T(h)17-cytokines
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Kanagawa, Japan
    PLoS Biol 10:e1001255. 2012
  3. doi request reprint Induced pluripotency as a potential path towards iNKT cell-mediated cancer immunotherapy
    Hiroshi Watarai
    RIKEN Center for Allergy and Immunology, 1 7 22 Suehiro cho, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
    Int J Hematol 95:624-31. 2012
  4. ncbi request reprint Plasma membrane-focused proteomics: dramatic changes in surface expression during the maturation of human dendritic cells
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan
    Proteomics 5:4001-11. 2005
  5. pmc A novel subset of mouse NKT cells bearing the IL-17 receptor B responds to IL-25 and contributes to airway hyperreactivity
    Asuka Terashima
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa, Japan
    J Exp Med 205:2727-33. 2008
  6. pmc IL-21-induced Bepsilon cell apoptosis mediated by natural killer T cells suppresses IgE responses
    Michishige Harada
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230 0045, Japan
    J Exp Med 203:2929-37. 2006
  7. doi request reprint Induction of Th1-biased cytokine production by alpha-carba-GalCer, a neoglycolipid ligand for NKT cells
    Takuya Tashiro
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Tsurumi, Yokohama, Japan
    Int Immunol 22:319-28. 2010
  8. pmc Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Hiroshi Watarai
    RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230 0045, Japan
    J Clin Invest 120:2610-8. 2010
  9. doi request reprint RCAI-17, 22, 24-26, 29, 31, 34-36, 38-40, and 88, the analogs of KRN7000 with a sulfonamide linkage: their synthesis and bioactivity for mouse natural killer T cells to produce Th2-biased cytokines
    Takuya Tashiro
    Glycosphingolipid Synthesis Group, Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Hirosawa 2 1, Wako Shi, Saitama 351 0198, Japan
    Bioorg Med Chem 16:8896-906. 2008
  10. doi request reprint Generation of functional NKT cells in vitro from embryonic stem cells bearing rearranged invariant Valpha14-Jalpha18 TCRalpha gene
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Kanagawa, Japan
    Blood 115:230-7. 2010

Collaborators

Detail Information

Publications29

  1. pmc PDC-TREM, a plasmacytoid dendritic cell-specific receptor, is responsible for augmented production of type I interferon
    Hiroshi Watarai
    Laboratories for Immune Regulation and Host Defense, RIKEN Research Center for Allergy and Immunology, 1 7 22, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Proc Natl Acad Sci U S A 105:2993-8. 2008
    ..Thus, PDC-TREM is responsible for IFN-alpha production, whereas TLR signals are essential for PDC-TREM expression...
  2. pmc Development and function of invariant natural killer T cells producing T(h)2- and T(h)17-cytokines
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Kanagawa, Japan
    PLoS Biol 10:e1001255. 2012
    ..In this study we demonstrated that the IL-17RB⁺iNKT cell subsets develop distinct from classical iNKT cell developmental stages in the thymus and play important roles in the pathogenesis of airway diseases...
  3. doi request reprint Induced pluripotency as a potential path towards iNKT cell-mediated cancer immunotherapy
    Hiroshi Watarai
    RIKEN Center for Allergy and Immunology, 1 7 22 Suehiro cho, Tsurumi, Yokohama, Kanagawa 230 0045, Japan
    Int J Hematol 95:624-31. 2012
    ....
  4. ncbi request reprint Plasma membrane-focused proteomics: dramatic changes in surface expression during the maturation of human dendritic cells
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan
    Proteomics 5:4001-11. 2005
    ..Whereas 106 proteins were selectively expressed in immature cells or down-regulated after maturation, 191 proteins were selectively expressed in mature cells or up-regulated after maturation...
  5. pmc A novel subset of mouse NKT cells bearing the IL-17 receptor B responds to IL-25 and contributes to airway hyperreactivity
    Asuka Terashima
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa, Japan
    J Exp Med 205:2727-33. 2008
    ..These results strongly suggest that IL-17RB(+) CD4(+) NKT cells play a crucial role in the pathogenesis of asthma...
  6. pmc IL-21-induced Bepsilon cell apoptosis mediated by natural killer T cells suppresses IgE responses
    Michishige Harada
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230 0045, Japan
    J Exp Med 203:2929-37. 2006
    ..BCG-activated PBMCs significantly reduce IgE production by human B cells. These findings provide new insight into the therapeutic effect of BCG in allergic diseases...
  7. doi request reprint Induction of Th1-biased cytokine production by alpha-carba-GalCer, a neoglycolipid ligand for NKT cells
    Takuya Tashiro
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Tsurumi, Yokohama, Japan
    Int Immunol 22:319-28. 2010
    ..Modulation of the sugar moiety in glycolipids might therefore provide optimal therapeutic reagents for protective immune responses against tumor or pathogens...
  8. pmc Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Hiroshi Watarai
    RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230 0045, Japan
    J Clin Invest 120:2610-8. 2010
    ..These studies demonstrate the feasibility of expanding functionally competent NKT cells via an iPSC phase, an approach that may be adapted for NKT cell-targeted therapy in humans...
  9. doi request reprint RCAI-17, 22, 24-26, 29, 31, 34-36, 38-40, and 88, the analogs of KRN7000 with a sulfonamide linkage: their synthesis and bioactivity for mouse natural killer T cells to produce Th2-biased cytokines
    Takuya Tashiro
    Glycosphingolipid Synthesis Group, Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Hirosawa 2 1, Wako Shi, Saitama 351 0198, Japan
    Bioorg Med Chem 16:8896-906. 2008
    ..According to their bioassay, these sulfonamide analogs were shown to be the stimulants of mouse NKT cells to induce the production of Th2-biased cytokines in vitro, while RCAI-38 did not induce any cytokine production...
  10. doi request reprint Generation of functional NKT cells in vitro from embryonic stem cells bearing rearranged invariant Valpha14-Jalpha18 TCRalpha gene
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Kanagawa, Japan
    Blood 115:230-7. 2010
    ..The cloned ES culture system offers a new opportunity for the elucidation of the molecular events on NKT-cell development and for the establishment of NKT-cell therapy...
  11. pmc The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells
    Yasutaka Motomura
    Laboratory for Signal Network, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan
    Nat Immunol 12:450-9. 2011
    ..Our results indicate that E4BP4 has multiple functions in controlling the plasticity of IL-13 in T(H)1 cells and IL-10 in T(H)1 cells, T(H)2 cells, T(reg) cells and NKT cells...
  12. ncbi request reprint Induction of regulatory properties in dendritic cells by Valpha14 NKT cells
    Satoshi Kojo
    Laboratory for Immune Regulation, Research Center for Allergy and Immunology RCAI, Institute of Physical and Chemical Research RIKEN, Kanagawa, Japan
    J Immunol 175:3648-55. 2005
    ..These findings contribute to explaining how Valpha14 NKT cells regulate the immune responses in vivo...
  13. doi request reprint Synthesis and biological activity of hydroxylated analogues of KRN7000 (α-galactosylceramide)
    Masao Shiozaki
    Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Wako Shi, Saitama, Japan
    Carbohydr Res 370:46-66. 2013
    ..It was recognized that two compounds (†)RCAI-147/-160 showed good suppression of EAE symptoms...
  14. pmc Identification of CD4(-)CD8(-) double-negative natural killer T cell precursors in the thymus
    Nyambayar Dashtsoodol
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa, Japan
    PLoS ONE 3:e3688. 2008
    ..However, the developmental progression of NKT cells in the earlier stages remains unclear, and the possible existence of NKT cell presursors in the earlier stages than DP stage remains to be tested...
  15. doi request reprint RCAI-61 and related 6'-modified analogs of KRN7000: their synthesis and bioactivity for mouse lymphocytes to produce interferon-γ in vivo
    Takuya Tashiro
    Glycosphingolipid Synthesis Group, Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Hirosawa 2 1, Wako, Saitama 351 0198, Japan
    Bioorg Med Chem 21:3066-79. 2013
    ....
  16. doi request reprint RCAI-84, 91, and 105-108, ureido and thioureido analogs of KRN7000: their synthesis and bioactivity for mouse lymphocytes to produce Th1-biased cytokines
    Takuya Tashiro
    Glycosphingolipid Synthesis Group, Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Hirosawa 2 1, Wako Shi, Saitama 351 0198, Japan
    Bioorg Med Chem 20:4540-8. 2012
    ..The hexadecyl ureido analog [RCAI-84 (2)] was comparable to KRN7000 in its bioactivity. The octylureido [RCAI-107 (3)], 5-phenylpentylureido [RCAI-108 (5)], and thioureido [RCAI-91 (6)] analogs were almost inactive...
  17. ncbi request reprint The importance of CD25+ CD4+ regulatory T cells in mouse hepatic allograft tolerance
    Xiaofeng Jiang
    RIKEN Research Center for Allergy and Immunology, Yokohama City, Kanagawa, Japan
    Liver Transpl 12:1112-8. 2006
    ..In conclusion, these results indicate that CD25+ CD4+ regulatory T cells in the recipient but not in the donor of liver transplantation are important for the tolerance induction...
  18. doi request reprint Synthesis and biological activity of ester and ether analogues of alpha-galactosylceramide (KRN7000)
    Masao Shiozaki
    Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Hirosawa 2 1, Wako Shi, Saitama 351 0198, Japan
    Carbohydr Res 345:1663-84. 2010
    ..Ester analogues 30a, 31a, and 61 showed activity for IFNgamma and IL-4 production of iNKT cells, while ether (31b) and 4-methoxy ester (76) analogues of alpha-galactosylceramide were not active for iNKT cells...
  19. doi request reprint RCAI-37, 56, 59, 60, 92, 101, and 102, cyclitol and carbasugar analogs of KRN7000: their synthesis and bioactivity for mouse lymphocytes to produce Th1-biased cytokines
    Takuya Tashiro
    Glycosphingolipid Synthesis Group, Laboratory for Immune Regulation, Research Center for Allergy and Immunology, RIKEN, Hirosawa 2 1, Wako Shi, Saitama 351 0198, Japan
    Bioorg Med Chem 17:6360-73. 2009
    ..RCAI-60 (4, carbafucose analog) and RCAI-101 (6, 6-O-methylated 3) showed strong bioactivity, on the other hands, RCAI-37 (1, myo-inositol analog) and 102 (2, neo-inositol analog) induced little cytokine production...
  20. pmc IkappaB kinase beta-induced phosphorylation of CARMA1 contributes to CARMA1 Bcl10 MALT1 complex formation in B cells
    Hisaaki Shinohara
    Laboratory for Lymphocyte Differentiation, RIKEN Research Center for Allergy and Immunology, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    J Exp Med 204:3285-93. 2007
    ..The activated IKKbeta does not merely function as an effector enzyme but also modifies the upstream signaling complex through a feedback mechanism, thereby optimizing the strength and duration of the nuclear factor kappaB signal...
  21. doi request reprint The specialized iNKT cell system recognizes glycolipid antigens and bridges the innate and acquired immune systems with potential applications for cancer therapy
    Masaru Taniguchi
    Laboratory of Immune Regulation, RIKEN Research Center for Allergy and Immunology, 1 7 22, Suehiro cho, Tsurumi ku, Yokohama, Japan
    Int Immunol 22:1-6. 2010
    ..On the basis of these mechanisms, iNKT cell-targeted adjuvant cell therapies have been developed and have shown great promise in initial clinical trials on cancer patients...
  22. doi request reprint Methods for detection, isolation and culture of mouse and human invariant NKT cells
    Hiroshi Watarai
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, 1 7 22, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa, 230 0045, Japan
    Nat Protoc 3:70-8. 2008
    ..In the case of isolation of human peripheral blood mononuclear cells (PBMCs) from whole blood, it takes 2 h and requires 5 ml of blood to obtain 5 x 10(6) PBMCs, which contain 500-25,000 iNKT cells...
  23. doi request reprint M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex
    Koji Hase
    Laboratory for Epithelial Immunobiology, Kanagawa 230 0045, Japan
    Nat Cell Biol 11:1427-32. 2009
    ..Our results reveal that M-Sec functions as a key regulator of membrane nanotube formation through interaction with the Ral-exocyst pathway...
  24. pmc Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress
    Wakana Ohashi
    RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, Japan
    PLoS Genet 12:e1006349. 2016
    ..Therapeutic targeting of ZIP7 could lead to effective treatment of gastrointestinal disorders...
  25. ncbi request reprint Construction of an open-access database that integrates cross-reference information from the transcriptome and proteome of immune cells
    Atsushi Hijikata
    RIKEN Research Center for Allergy and Immunology, 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, Kanagawa, Japan
    Bioinformatics 23:2934-41. 2007
    ..To address this, we constructed an open-access database that enabled us to cross-reference transcriptomic and proteomic data obtained from immune cells...
  26. ncbi request reprint Cutting edge: critical role of CXCL16/CXCR6 in NKT cell trafficking in allograft tolerance
    Xiaofeng Jiang
    Laboratory for Immune Regulation, RIKEN Research Center for Allergy and Immunology, Kanagawa, Japan
    J Immunol 175:2051-5. 2005
    ..These results demonstrate the unique role of CXCL16 and CXCR6 molecules in the maintenance of cardiac allograft tolerance mediated by NKT cells...
  27. pmc PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1
    Hisaaki Shinohara
    Laboratory for Lymphocyte Differentiation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230 0045, Japan
    J Exp Med 202:1423-31. 2005
    ..Hence, our data suggest that phosphorylation of CARMA1, mediated by PKCbeta, brings two key protein kinases, TAK1 and IKK, into close proximity, thereby allowing TAK1 to phosphorylate IKK...
  28. pmc Regulation of T helper type 2 cell differentiation by murine Schnurri-2
    Motoko Y Kimura
    Department of Immunology, Graduate School of Medicine, Chiba University, Chiba 260 8670, Japan
    J Exp Med 201:397-408. 2005
    ..Thus, Shn-2 plays a crucial role in the control of Th2 cell differentiation by regulating NF-kappaB function...
  29. ncbi request reprint Crystallization of the functional domain of human thrombopoietin using an antigen-binding fragment derived from neutralizing monoclonal antibody
    Ryota Kuroki
    Central Laboratories for Key Technology, Kirin Brewery Co Ltd, 1 13 5 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    Acta Crystallogr D Biol Crystallogr 58:856-8. 2002
    ..71, b = 46.48, c = 184.63 A, beta = 90.42 degrees. The results of a molecular-replacement analysis indicate that the Fab molecules interact with each other and provide a suitable interface for crystallization...