Yoshiki Sasai

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. pmc Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity
    Hiroshi Kawasaki
    Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 99:1580-5. 2002
  2. ncbi request reprint An essential role of Xenopus Foxi1a for ventral specification of the cephalic ectoderm during gastrulation
    Mami Matsuo-Takasaki
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Development 132:3885-94. 2005
  3. ncbi request reprint Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of coculture and neurosphere methods
    Asuka Morizane
    Department of Neurosurgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
    J Neurosci Res 83:1015-27. 2006
  4. ncbi request reprint A matter of some importins: nuclear transport factors in ES cell maintenance and differentiation
    Yoshiki Sasai
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Dev Cell 12:172-4. 2007
  5. doi request reprint Next-generation regenerative medicine: organogenesis from stem cells in 3D culture
    Yoshiki Sasai
    Neurogenesis and Organogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell Stem Cell 12:520-30. 2013
  6. doi request reprint Cytosystems dynamics in self-organization of tissue architecture
    Yoshiki Sasai
    Neurogenesis and Organogenesis Group, RIKEN Center for Development Biology, Kobe 650 0047, Japan
    Nature 493:318-26. 2013
  7. doi request reprint In vitro organogenesis in three dimensions: self-organising stem cells
    Yoshiki Sasai
    Neurogenesis and Organogenesis Group, RIKEN Center for Developmental Biology, Kobe, Japan
    Development 139:4111-21. 2012
  8. doi request reprint Bridging the gap from frog research to human therapy: a tale of neural differentiation in Xenopus animal caps and human pluripotent cells
    Yoshiki Sasai
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Dev Growth Differ 50:S47-55. 2008
  9. pmc Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation
    Takafumi Wataya
    Organogenesis and Neurogenesis Group and Division of Human Stem Cell Technology, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Proc Natl Acad Sci U S A 105:11796-801. 2008
  10. doi request reprint Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals
    Teruko Danjo
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    J Neurosci 31:1919-33. 2011

Detail Information

Publications84

  1. pmc Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity
    Hiroshi Kawasaki
    Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 99:1580-5. 2002
    ..The SDIA method provides an unlimited source of primate cells for the study of pathogenesis, drug development, and transplantation in degenerative diseases such as Parkinson's disease and retinitis pigmentosa...
  2. ncbi request reprint An essential role of Xenopus Foxi1a for ventral specification of the cephalic ectoderm during gastrulation
    Mami Matsuo-Takasaki
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Development 132:3885-94. 2005
    ..Thus, Xfoxi1a is an essential regulator of ventral specification of the early head ectoderm during gastrulation...
  3. ncbi request reprint Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of coculture and neurosphere methods
    Asuka Morizane
    Department of Neurosurgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
    J Neurosci Res 83:1015-27. 2006
    ..These findings suggest that progenitors of DA neurons are generated and can proliferate in ES cell-derived neurospheres induced by serial SDIA and PA6 conditioned medium treatment...
  4. ncbi request reprint A matter of some importins: nuclear transport factors in ES cell maintenance and differentiation
    Yoshiki Sasai
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Dev Cell 12:172-4. 2007
    ..The authors show how switching of importin-alpha subtypes exerts a selective gate-keeping function in the nuclear import of key transcription factors that regulate stem cell maintenance and differentiation...
  5. doi request reprint Next-generation regenerative medicine: organogenesis from stem cells in 3D culture
    Yoshiki Sasai
    Neurogenesis and Organogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell Stem Cell 12:520-30. 2013
    ..This new area of stem cell research-the spatiotemporal control of dynamic cellular interactions-will open a new avenue for next-generation regenerative medicine...
  6. doi request reprint Cytosystems dynamics in self-organization of tissue architecture
    Yoshiki Sasai
    Neurogenesis and Organogenesis Group, RIKEN Center for Development Biology, Kobe 650 0047, Japan
    Nature 493:318-26. 2013
    ....
  7. doi request reprint In vitro organogenesis in three dimensions: self-organising stem cells
    Yoshiki Sasai
    Neurogenesis and Organogenesis Group, RIKEN Center for Developmental Biology, Kobe, Japan
    Development 139:4111-21. 2012
    ..We also attempt to interpret the results of previous in vivo studies of retinal development in light of the self-organising nature of the retina...
  8. doi request reprint Bridging the gap from frog research to human therapy: a tale of neural differentiation in Xenopus animal caps and human pluripotent cells
    Yoshiki Sasai
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Dev Growth Differ 50:S47-55. 2008
    ..In this commentary, I describe these issues with a particular emphasis on the contributions made by Japanese scientists...
  9. pmc Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation
    Takafumi Wataya
    Organogenesis and Neurogenesis Group and Division of Human Stem Cell Technology, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Proc Natl Acad Sci U S A 105:11796-801. 2008
    ..These findings indicate that, instead of the addition of inductive signals, minimization of exogenous patterning signaling plays a key role in rostral hypothalamic specification of neural progenitors derived from pluripotent cells...
  10. doi request reprint Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals
    Teruko Danjo
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    J Neurosci 31:1919-33. 2011
    ..We discuss a model of the antagonistic Fgf8 and Fgf15/19 signaling in rostral-caudal subpallial patterning and compare it with the roles of these molecules in cortical patterning...
  11. pmc Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells
    Hanako Ikeda
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Proc Natl Acad Sci U S A 102:11331-6. 2005
    ..Taken together, ES cells treated with SFEB/Dkk1/LeftyA/serum/activin generate neural retinal precursors, which have the competence of photoreceptor differentiation...
  12. doi request reprint Intrinsic transition of embryonic stem-cell differentiation into neural progenitors
    Daisuke Kamiya
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Nature 470:503-9. 2011
    ..Thus, the transition of ES cell differentiation from the epiblast state into neuroectodermal progenitors specifically depends on the cell-intrinsic expression and activator function of Zfp521...
  13. doi request reprint Cv2, functioning as a pro-BMP factor via twisted gastrulation, is required for early development of nephron precursors
    Makoto Ikeya
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Dev Biol 337:405-14. 2010
    ..These findings revealed the molecular hierarchy between extracellular modifiers that orchestrate local BMP signal peaks in the organogenetic microenvironment...
  14. ncbi request reprint Generation of cerebellar neuron precursors from embryonic stem cells
    Hong Lin Su
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Dev Biol 290:287-96. 2006
    ..This is the first report of in vitro recapitulation of early differentiation of cerebellar neurons by using the ES cell system...
  15. doi request reprint Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals
    Mototsugu Eiraku
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell Stem Cell 3:519-32. 2008
    ..Thus, spatial and temporal aspects of early corticogenesis are recapitulated and can be manipulated in this ESC culture...
  16. pmc Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex
    Taisuke Kadoshima
    Laboratory of Organogenesis and Neurogenesis and Four Dimensional Tissue Analysis Unit, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Proc Natl Acad Sci U S A 110:20284-9. 2013
    ..Thus, human neocorticogenesis involves intrinsic programs that enable the emergence of complex neocortical features. ..
  17. doi request reprint Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells
    Keiko Muguruma
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe, Japan
    Nat Neurosci 13:1171-80. 2010
    ..This selective preparation of bona fide Purkinje cells should aid future investigation of this important neuron...
  18. doi request reprint Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin
    Eri Mizuhara
    Group for Neuronal Differentiation, KAN Research Institute Inc, Kobe, Hyogo 650 0047, Japan
    Dev Biol 338:202-14. 2010
    ..Taken together, our results identify two spatially demarcated subregions that generate distinct cerebellar GABAergic subtypes and reveal the origin of PCs in the ventricular zone of the cerebellar primordium...
  19. doi request reprint Self-formation of optic cups and storable stratified neural retina from human ESCs
    Tokushige Nakano
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell Stem Cell 10:771-85. 2012
    ..This storage method at an intermediate step during the time-consuming differentiation process provides a versatile solution for quality control in large-scale preparation of clinical-grade retinal tissues...
  20. doi request reprint Jiraiya attenuates BMP signaling by interfering with type II BMP receptors in neuroectodermal patterning
    Toshihiro Aramaki
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Dev Cell 19:547-61. 2010
    ..Thus, Jiraiya represents a cell-intrinsic cutoff mechanism for dynamic responsiveness to BMP signals via subtype-selective receptor control...
  21. ncbi request reprint Directed differentiation of telencephalic precursors from embryonic stem cells
    Kiichi Watanabe
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Nat Neurosci 8:288-96. 2005
    ..Thus, in the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals...
  22. ncbi request reprint Functional analysis of chick ONT1 reveals distinguishable activities among olfactomedin-related signaling factors
    Makoto Sakuragi
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Mech Dev 123:114-23. 2006
    ..Thus, distinct Olfactomedin-related factors evoke qualitatively different phenotypes even in the same experimental systems, suggesting that Olfactomedin family uses multiple response systems to mediate its signals in embryogenesis...
  23. doi request reprint Ectodermal factor restricts mesoderm differentiation by inhibiting p53
    Noriaki Sasai
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell 133:878-90. 2008
    ..Thus, XFDL156 actively restricts mesodermal differentiation in the presumptive ectoderm by controlling the spatiotemporal responsiveness to p53...
  24. pmc Activation of Wnt/ß-catenin signaling in ESC promotes rostral forebrain differentiation in vitro
    Nozomu Takata
    Laboratory for in vitro Histogenesis, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuou ku, Kobe, Hyogo, 650 0047, Japan
    In Vitro Cell Dev Biol Anim 52:374-82. 2016
    ..Wnt-priming culture method may provide a useful tool for applications in the areas of basic science and molecular therapeutics for regenerative medicine. ..
  25. pmc Neural conversion of ES cells by an inductive activity on human amniotic membrane matrix
    Morio Ueno
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Proc Natl Acad Sci U S A 103:9554-9. 2006
    ....
  26. doi request reprint Robust stability of the embryonic axial pattern requires a secreted scaffold for chordin degradation
    Hidehiko Inomata
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Cell 134:854-65. 2008
    ..These results indicate that stable axial formation depends on two compensatory regulatory pathways involving ONT1/B1TP and dorsally expressed BMPs...
  27. ncbi request reprint The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo
    Akiko Arakawa
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Dev Biol 306:160-9. 2007
    ..Thus, xDel1 functions as a unique extracellular regulatory factor of DV patterning in early vertebrate embryogenesis...
  28. doi request reprint Self-formation of functional adenohypophysis in three-dimensional culture
    Hidetaka Suga
    Neurogenesis and Organogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Nature 480:57-62. 2011
    ..Thus, functional anterior pituitary tissue self-forms in ES cell culture, recapitulating local tissue interactions...
  29. ncbi request reprint Neural crest determination by co-activation of Pax3 and Zic1 genes in Xenopus ectoderm
    Takahiko Sato
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Development 132:2355-63. 2005
    ..Thus, co-activation of Pax3 and Zic1, in concert with Wnt, plays a decisive role for early neural crest determination in the correct place of the Xenopus ectoderm...
  30. ncbi request reprint [Human pluripotent stem cell and neural differentiation]
    Takafumi Wataya
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Brain Nerve 60:1165-72. 2008
    ..It is important that researchers in the basic sciences and clinical medicine should discuss these problems together to overcome both scientific and ethical issues related to stem cells...
  31. ncbi request reprint Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis
    Makoto Ikeya
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Development 133:4463-73. 2006
    ..This study demonstrates essential pro-Bmp functions of Cv2 for locally restricted signal enhancement in multiple aspects of mammalian organogenesis...
  32. doi request reprint Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells
    Masatoshi Ohgushi
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell Stem Cell 7:225-39. 2010
    ..Thus, the Abr-dependent "Rho-high/Rac-low" state plays a decisive role in initiating the dissociation-induced actomyosin hyperactivation and apoptosis in hESCs...
  33. pmc XTsh3 is an essential enhancing factor of canonical Wnt signaling in Xenopus axial determination
    Takayuki Onai
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Chuo, Kobe, Japan
    EMBO J 26:2350-60. 2007
    ..We discuss the role of XTsh3 as an essential amplifying factor of canonical Wnt signaling in embryonic dorsal determination...
  34. doi request reprint Anterior neural development requires Del1, a matrix-associated protein that attenuates canonical Wnt signaling via the Ror2 pathway
    Akira Takai
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan
    Development 137:3293-302. 2010
    ..These findings indicate that the ECM protein Del1 promotes forebrain development by creating a local environment that attenuates the cellular response to posteriorizing Wnt signals via a unique pathway...
  35. doi request reprint In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction
    Fumitaka Osakada
    Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Chuo Ku, Kobe, Japan
    J Cell Sci 122:3169-79. 2009
    ..This method provides a solution to the problem of cross-species antigenic contamination in cell-replacement therapy, and is also useful for in vitro modeling of development, disease and drug screening...
  36. pmc Relaxation-expansion model for self-driven retinal morphogenesis: a hypothesis from the perspective of biosystems dynamics at the multi-cellular level
    Mototsugu Eiraku
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe, Japan
    Bioessays 34:17-25. 2012
    ..This model involves three consecutive local rules (relaxation, apical constriction, and expansion), and its computer simulation recapitulates the optic-cup morphogenesis in silico...
  37. doi request reprint Pericellular matrix of decidua-derived mesenchymal cells: a potent human-derived substrate for the maintenance culture of human ES cells
    Tomoko Nagase
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan
    Dev Dyn 238:1118-30. 2009
    ..Since decidual mesenchymal cells can be prepared and expanded in a large quantity, PCM-DM is a practical human-derived substitute for the animal-derived substrates for use in clinical-grade culture of hES cells...
  38. doi request reprint Twisted gastrulation mutation suppresses skeletal defect phenotypes in Crossveinless 2 mutant mice
    Makoto Ikeya
    Organogenesis and Neurogenesis Group, RIKEN, Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Mech Dev 125:832-42. 2008
    ..We also present genetic evidence for the context-dependent functional relationship between Tsg and Cv2 during mouse development...
  39. ncbi request reprint Emergence of dorsal-ventral polarity in ESC-derived retinal tissue
    Yuiko Hasegawa
    Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, 2 2 3, Minatojima Minamimachi, Chuo, Kobe 650 0047, Japan
    Development 143:3895-3906. 2016
    ..These observations suggest that D-V polarity could be established within the self-formed retinal neuroepithelium by intrinsic mechanisms involving the spatiotemporal regulation of canonical Wnt and BMP signals...
  40. pmc FGFR1-Frs2/3 signalling maintains sensory progenitors during inner ear hair cell formation
    Kazuya Ono
    Sensory Development, RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan Neurogenesis and Organogenesis, RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan
    PLoS Genet 10:e1004118. 2014
    ..We suggest that FGF signaling, through the activation of MAP kinase, is necessary for the maintenance of sensory progenitors and commits precursors to sensory cell differentiation in the mammalian cochlea. ..
  41. pmc Robust formation and maintenance of continuous stratified cortical neuroepithelium by laminin-containing matrix in mouse ES cell culture
    Makoto Nasu
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan
    PLoS ONE 7:e53024. 2012
    ..Thus, our results indicate that laminin-entactin ECM promotes the formation of structurally stable telencephalic tissues in 3D ESC culture, and supports the morphogenetic recapitulation of cortical development...
  42. ncbi request reprint Distinct effects of caudalizing factors on regional specification of embryonic stem cell-derived neural precursors
    Takashi Irioka
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Brain Res Dev Brain Res 154:63-70. 2005
    ..These findings provide useful information on the proper selection of combination of signaling molecules, doses and timing for steering ES cell differentiation by caudalizing factors into caudal neural fates...
  43. doi request reprint Scaling of dorsal-ventral patterning by embryo size-dependent degradation of Spemann's organizer signals
    Hidehiko Inomata
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Cell 153:1296-311. 2013
    ..We propose a scaling model that enables the DV pattern to adjust proportionally to embryonic axis size...
  44. doi request reprint Stepwise differentiation of pluripotent stem cells into retinal cells
    Fumitaka Osakada
    Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe, Japan
    Nat Protoc 4:811-24. 2009
    ..It will also improve our understanding of the development of the central nervous system, especially the eye...
  45. doi request reprint Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells
    Fumitaka Osakada
    Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Nat Biotechnol 26:215-24. 2008
    ..Subsequent treatment with retinoic acid and taurine induced photoreceptor differentiation. These findings may facilitate the development of human ES cell-based transplantation therapies for retinal diseases...
  46. ncbi request reprint Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations
    Makoto Ikeya
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan
    Int J Dev Biol 49:807-23. 2005
    ..In addition, this method is applicable to rapid generation of transgenic vectors, demonstrating its versatility in reverse genetic studies...
  47. doi request reprint Bidirectional developmental potential in reprogrammed cells with acquired pluripotency
    Haruko Obokata
    1 Laboratory for Cellular Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan 2 Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan 3 Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 505:676-80. 2014
    ..Taken together, the developmental potential of STAP cells, shown by chimaera formation and in vitro cell conversion, indicates that they represent a unique state of pluripotency. ..
  48. ncbi request reprint Rapid accumulation of nucleostemin in nucleolus during newt regeneration
    Nobuyasu Maki
    Center for Developmental Biology, RIKEN Kobe, Chuo Ku, Kobe, Japan
    Dev Dyn 236:941-50. 2007
    ..These findings suggest that nucleostemin plays a role in the dedifferentiation of newt cells and can provide crucial clues for addressing the molecular events at early steps of cellular dedifferentiation in newts...
  49. pmc Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue
    Hideya Sakaguchi
    Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Nat Commun 6:8896. 2015
    ..Thus, we have developed an in vitro model that recapitulates human hippocampus development, allowing the generation of functional hippocampal granule- and pyramidal-like neurons. ..
  50. pmc Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells
    Chikafumi Ozone
    Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Nat Commun 7:10351. 2016
    ..Thus, we report a useful methodology for the production of regulator-responsive human pituitary tissue that may benefit future studies in regenerative medicine. ..
  51. doi request reprint Self-organizing optic-cup morphogenesis in three-dimensional culture
    Mototsugu Eiraku
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Nature 472:51-6. 2011
    ..We demonstrate that optic-cup morphogenesis in this simple cell culture depends on an intrinsic self-organizing program involving stepwise and domain-specific regulation of local epithelial properties...
  52. ncbi request reprint A ROCK inhibitor permits survival of dissociated human embryonic stem cells
    Kiichi Watanabe
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Nat Biotechnol 25:681-6. 2007
    ..We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells...
  53. doi request reprint In vitro recapitulation of neural development using embryonic stem cells: from neurogenesis to histogenesis
    Keiko Muguruma
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan
    Dev Growth Differ 54:349-57. 2012
    ..We also discuss how in vitro model systems using ES cells can shed new light on the mechanistic understanding of organogenesis, taking an example of recent progress in self-organizing histogenesis...
  54. doi request reprint Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues
    Mototsugu Eiraku
    Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe, Japan
    Nat Protoc 7:69-79. 2011
    ..In contrast, the cortical differentiation culture can be started without exogenous extracellular matrix proteins, and it generates stratified cortical epithelia consisting of four distinct layers in 13 d...
  55. pmc Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration
    Hiroshi Shirai
    Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Chuo, Kobe 650 0047, Japan Department of Ophthalmology, Nagoya University Graduate School of Medicine, Showa, Nagoya 466 8550, Aichi, Japan
    Proc Natl Acad Sci U S A 113:E81-90. 2016
    ..The findings of this study demonstrate the clinical feasibility of hESC-retina transplantation and provide the practical tools for the optimization of transplantation strategies for future clinical applications. ..
  56. doi request reprint Lonely death dance of human pluripotent stem cells: ROCKing between metastable cell states
    Masatoshi Ohgushi
    Unit for Human Stem Cell Technology, RIKEN Center for Developmental Biology, Kobe, 650 0047, Japan
    Trends Cell Biol 21:274-82. 2011
    ..We discuss possible links of the metastable pluripotent state to ROCK-dependent human embryonic stem cell apoptosis and summarize recent progress in molecular understandings of this phenomenon...
  57. doi request reprint Modeling cell apoptosis for simulating three-dimensional multicellular morphogenesis based on a reversible network reconnection framework
    Satoru Okuda
    Laboratory for in vitro Histogenesis, Center for Developmental Biology CDB, RIKEN, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo, 650 0047, Japan
    Biomech Model Mechanobiol 15:805-16. 2016
    ..Thus, the proposed model successfully provided a basis for expressing apoptotic cell behaviors during 3D multicellular morphogenesis based on an RNR model framework. ..
  58. doi request reprint IGF-2/IGF-1R signaling has distinct effects on Sox1, Irx3, and Six3 expressions during ES cell derived-neuroectoderm development in vitro
    Nozomu Takata
    Laboratory for in vitro Histogenesis, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuou ku, Kobe, Hyogo, 650 0047, Japan
    In Vitro Cell Dev Biol Anim 52:607-15. 2016
    ..Together, our results demonstrate that IGF-2/IGF-1R signaling has different effects on neural marker expression, which may influence the early regional identity of ES cell-derived neural tissues. ..
  59. doi request reprint Vulnerability of Purkinje Cells Generated from Spinocerebellar Ataxia Type 6 Patient-Derived iPSCs
    Yoshihito Ishida
    Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Laboratory for Cell Asymmetry, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Drug Discovery and Disease Research Laboratory, Shionogi and Co, Ltd, Osaka 561 0825, Japan
    Cell Rep 17:1482-1490. 2016
    ..Thus, we have constructed an in vitro disease model recapitulating both ontogenesis and pathogenesis. This model may be useful for pathogenic investigation and drug screening...
  60. pmc Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice
    Juthaporn Assawachananont
    Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Stem Cell Reports 2:662-74. 2014
    ..We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept" for retinal sheet transplantation therapy for advanced retinal degenerative diseases. ..
  61. ncbi request reprint The neurotrophin-receptor-related protein NRH1 is essential for convergent extension movements
    Noriaki Sasai
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN 2 2 3 Minatojima minamimachi, Chuo Kobe 650 0047, Japan
    Nat Cell Biol 6:741-8. 2004
    ..This demonstrates an essential role for p75(NTR)-related signalling in early embryonic morphogenesis...
  62. doi request reprint Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue
    Atsushi Kuwahara
    1 Neurogenesis and Organogenesis Group, RIKEN Center for Developmental Biology, 2 2 3 Manatojima Minamimachi, Chuo, Kobe 650 0047, Japan 2 Human Stem Cell Technology Unit, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo, Kobe 650 0047, Japan 3 Environmental Health Science Laboratory, Sumitomo Chemical Co, Ltd, 3 1 98 Kasugade naka, Konohana, Osaka 554 8558, Japan
    Nat Commun 6:6286. 2015
    ..We demonstrate that the NR-RPE boundary tissue further self-organizes a niche for CM stem cells that functions to expand the NR peripherally by de novo progenitor generation. ..
  63. doi request reprint Vertex dynamics simulations of viscosity-dependent deformation during tissue morphogenesis
    Satoru Okuda
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo, 650 0047, Japan
    Biomech Model Mechanobiol 14:413-25. 2015
    ..The proposed model will be useful for simulating dynamic deformation processes of tissue morphogenesis depending on viscous properties of various tissue components. ..
  64. doi request reprint Stimulus-triggered fate conversion of somatic cells into pluripotency
    Haruko Obokata
    1 Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA 2 Laboratory for Cellular Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan 3 Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Nature 505:641-7. 2014
    ..Thus, our findings indicate that epigenetic fate determination of mammalian cells can be markedly converted in a context-dependent manner by strong environmental cues. ..
  65. doi request reprint Self-formation of layered neural structures in three-dimensional culture of ES cells
    Mototsugu Eiraku
    Unit for Four Dimensional Tissue Analysis, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Curr Opin Neurobiol 22:768-77. 2012
    ..A simple in vitro system using ESCs is useful for elucidating mechanistic dynamics in the complex orchestration of neural development...
  66. doi request reprint Rho-Signaling-Directed YAP/TAZ Activity Underlies the Long-Term Survival and Expansion of Human Embryonic Stem Cells
    Masatoshi Ohgushi
    Unit for Human Stem Cell Technology, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Electronic address
    Cell Stem Cell 17:448-61. 2015
    ..Thus, our findings show that the capacity of hESCs to undergo long-term expansion in vitro is intrinsically coupled to their cellular identity through interconnected molecular circuits that link cell survival to pluripotency...
  67. pmc Comparative, transcriptome analysis of self-organizing optic tissues
    Munazah Andrabi
    Phyloinformatics Unit, RIKEN Center for Life Science Technologies, Kobe 650 0047, Japan
    Sci Data 2:150030. 2015
    ..This comparative dataset will help elucidate how Fgf and Wnt/β-catenin signaling affect gene expression during optic tissue differentiation and will help inform future efforts to optimize in vitro optic tissue culture technology. ..
  68. doi request reprint Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells
    Keiko Muguruma
    Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan Electronic address
    Cell Rep 10:537-50. 2015
    ..Thus, human-ESC-derived cerebellar progenitors exhibit substantial self-organizing potential for generating a polarized structure reminiscent of the early human cerebellum at the first trimester. ..
  69. pmc Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells
    Kenji Mizuseki
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047 Japan
    Proc Natl Acad Sci U S A 100:5828-33. 2003
    ..Thus, SDIA-treated ES cells generate naive precursors that have the competence of differentiating into the "full" dorsal-ventral range of neuroectodermal derivatives in response to patterning signals...
  70. ncbi request reprint Generation of dopaminergic neurons from embryonic stem cells
    Yoshiki Sasai
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 00047, Japan
    J Neurol 249:II41-4. 2002
    ..Induction of neurons with stromal cell-derived inducing activity may be a useful new method for basic neuroscience research and therapeutic applications, including cell transplantation therapy for Parkinson's disease...
  71. ncbi request reprint Xenopus XsalF: anterior neuroectodermal specification by attenuating cellular responsiveness to Wnt signaling
    Takayuki Onai
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Dev Cell 7:95-106. 2004
    ..These findings suggest that the forebrain/midbrain-specific gene XsalF negatively controls cellular responsiveness to posteriorizing Wnt signals by regulating region-specific GSK3beta and Tcf3 expression...
  72. ncbi request reprint Dorsalization of the neural tube by Xenopus tiarin, a novel patterning factor secreted by the flanking nonneural head ectoderm
    Hiroshi Tsuda
    Department of Medical Embryology and Neurobiology, Kyoto University, Sakyo, Kyoto 606 8507, Japan
    Neuron 33:515-28. 2002
    ..These findings show that Tiarin is a novel patterning signal candidate acting in the specification of the dorsal neural tube...
  73. ncbi request reprint [Regulatory mechanisms of early neural development: lessons from Xenopus, mouse and primate studies]
    Yoshiki Sasai
    Department of Medical Embryology and Neurobiology, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507
    Seikagaku 74:297-303. 2002
  74. ncbi request reprint In vitro and in vivo characterization of pigment epithelial cells differentiated from primate embryonic stem cells
    Masatoshi Haruta
    Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, Kyoto, Japan
    Invest Ophthalmol Vis Sci 45:1020-5. 2004
    ..To determine whether primate embryonic stem (ES) cell-derived pigment epithelial cells (ESPEs) have the properties and functions of retinal pigment epithelial (RPE) cells in vitro and in vivo...
  75. pmc Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model
    Yasushi Takagi
    Department of Neurosurgery, Kyoto University Graduate School of Medicine, Kyoto, Japan
    J Clin Invest 115:102-9. 2005
    ..Behavioral studies and functional imaging revealed that the transplanted cells functioned as DA neurons and attenuated MPTP-induced neurological symptoms...
  76. ncbi request reprint Developmental biology: a blank canvas no more
    Yoshiki Sasai
    Nature 435:433-4. 2005
  77. ncbi request reprint Fluorescence-activated cell sorting-based purification of embryonic stem cell-derived neural precursors averts tumor formation after transplantation
    Hitoshi Fukuda
    Department of Neurosurgery, Kyoto University Graduate School of Medicine, 54, Shogoinkawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Stem Cells 24:763-71. 2006
    ..These results indicate that Sox1-based cell sorting of neural precursors prevents graft-derived tumor formation after transplantation, providing a promising strategy for cell transplantation therapy of neurodegenerative disorders...
  78. ncbi request reprint One-step induction of neurons from mouse embryonic stem cells in serum-free media containing vitamin B12 and heparin
    Hironori Yamazoe
    Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo, Kyoto 606 8507, Japan
    Cell Transplant 15:135-45. 2006
    ..In addition, this method can facilitate the discovery of soluble factors and genes that can aid in the induction of the ES cell to its neural fate...
  79. ncbi request reprint Collection of neural inducing factors from PA6 cells using heparin solution and their immobilization on plastic culture dishes for the induction of neurons from embryonic stem cells
    Hironori Yamazoe
    Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Kyoto 606 8507, Japan
    Biomaterials 26:5746-54. 2005
    ..The former factors were effectively immobilized on the culture dish, but those that induce differentiation may not be. Further optimization is required...
  80. ncbi request reprint Pluripotency of reprogrammed somatic genomes in embryonic stem hybrid cells
    Masako Tada
    Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo ku, Kyoto, Japan
    Dev Dyn 227:504-10. 2003
    ..When transplanted into mouse brains, the dopaminergic neurons were successfully integrated and expressed tyrosine hydroxylase. Thus, it should be possible to produce personalized ES-like cells with the reprogrammed somatic genomes...
  81. ncbi request reprint Induction of the differentiation of lentoids from primate embryonic stem cells
    Sotaro Ooto
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Invest Ophthalmol Vis Sci 44:2689-93. 2003
    ..To produce lens cells from primate embryonic stem (ES) cells in a reproducible, controlled manner...
  82. ncbi request reprint Optimal conditions for in vivo induction of dopaminergic neurons from embryonic stem cells through stromal cell-derived inducing activity
    Asuka Morizane
    Department of Neurosurgery, Clinical Neuroscience, Kyoto University Graduate School of Medicine, Kyoto, Japan
    J Neurosci Res 69:934-9. 2002
    ..In conclusion, inducing DA neurons from mouse ES cells by SDIA for 12 days and grafting cell colonies into mouse striatum was the most effective method for the survival of TH-positive neurons in vivo...
  83. ncbi request reprint Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson's disease: effect of in vitro differentiation on graft survival and teratoma formation
    Anke Brederlau
    Institute of Anatomy and Cell Biology, Goteborg University, Gothenburg, Sweden
    Stem Cells 24:1433-40. 2006
    ..This indicates that prolonged in vitro differentiation of hESCs is essential for preventing formation of teratomas...
  84. doi request reprint Meningeal cells induce dopaminergic neurons from embryonic stem cells
    Hideki Hayashi
    Department of Biological Repair, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Eur J Neurosci 27:261-8. 2008
    ..These results indicate that meningeal cells can be used as feeders to induce DA neurons from ES cells, and that Wnt-5a plays an important role in DA neuron induction by SDIA...