Mikio Bakke

Summary

Affiliation: Research and Development Division
Country: Japan

Publications

  1. doi request reprint Identification, characterization, and molecular cloning of a novel hyaluronidase, a member of glycosyl hydrolase family 16, from Penicillium spp
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, Noda, Chiba, Japan
    FEBS Lett 585:115-20. 2011
  2. ncbi request reprint Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Appl Biochem Biotechnol 112:123-31. 2004
  3. ncbi request reprint Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    J Biotechnol 119:260-71. 2005
  4. ncbi request reprint Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biotechnol Bioeng 93:1023-7. 2006
  5. ncbi request reprint N-ethylmaleimide-resistant acyl-coenzyme A oxidase from Arthrobacter ureafaciens NBRC 12140: molecular cloning, gene expression and characterization of the recombinant enzyme
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biochim Biophys Acta 1774:65-71. 2007
  6. ncbi request reprint N1,N12-diacetylspermine oxidase from Debaryomyces hansenii T-42: purification, characterization, molecular cloning and gene expression
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biochim Biophys Acta 1774:1395-401. 2007
  7. ncbi request reprint Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Pref 278 0037, Noda City, Japan
    Arch Microbiol 180:227-31. 2003

Collaborators

  • Kozo Hirokawa
  • Keiko Gomi
  • Naoki Kajiyama

Detail Information

Publications7

  1. doi request reprint Identification, characterization, and molecular cloning of a novel hyaluronidase, a member of glycosyl hydrolase family 16, from Penicillium spp
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, Noda, Chiba, Japan
    FEBS Lett 585:115-20. 2011
    ..Regarding the substrate specificities, no chondroitinase and glucanase activities were detected. Judging from homology analyses and enzymatic properties, HAase-P seems to be a new type of HAase...
  2. ncbi request reprint Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Appl Biochem Biotechnol 112:123-31. 2004
    ..In addition, the conjugated enzyme showed decreased Km values for D-amino acids. Because of these outstanding characteristics, this new material is expected to be available for use as a liquid assay reagent...
  3. ncbi request reprint Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    J Biotechnol 119:260-71. 2005
    ..The recombinant enzyme was able to be used for the colorimetric determination of histamine, which gave a linear calibration curve and identical data with conventional methods...
  4. ncbi request reprint Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biotechnol Bioeng 93:1023-7. 2006
    ..The Km values of F42C for D-amino acids was about half of those of the wild-type enzyme. This mutant DAO with improved stability and affinity for its substrates is advantageous for the determination of D-amino acids...
  5. ncbi request reprint N-ethylmaleimide-resistant acyl-coenzyme A oxidase from Arthrobacter ureafaciens NBRC 12140: molecular cloning, gene expression and characterization of the recombinant enzyme
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biochim Biophys Acta 1774:65-71. 2007
    ..The recombinant enzyme was used for the colorimetric determination of free fatty acid, which gave a linear calibration...
  6. ncbi request reprint N1,N12-diacetylspermine oxidase from Debaryomyces hansenii T-42: purification, characterization, molecular cloning and gene expression
    Mikio Bakke
    Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278 0037, Japan
    Biochim Biophys Acta 1774:1395-401. 2007
    ..The gene encoding DASpmOX was cloned and expressed in Escherichia coli. The apparent k(cat) and K(m) values of recombinant enzyme for DASpm were found to be 158 s(-1) and 3.1 x 10(-4) M under the conditions described above, respectively...
  7. ncbi request reprint Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi
    Kozo Hirokawa
    Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Pref 278 0037, Noda City, Japan
    Arch Microbiol 180:227-31. 2003
    ..This enzyme could be useful for the measurement of hemoglobin A(1C), the N-terminal valine residue of the beta-subunit of which is glycated...