Tsutomu Shimada

Summary

Affiliation: Osaka Medical Center for Cancer and Cardiovascular Diseases
Country: Japan

Publications

  1. ncbi request reprint Selectivity of polycyclic inhibitors for human cytochrome P450s 1A1, 1A2, and 1B1
    T Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Chem Res Toxicol 11:1048-56. 1998
  2. ncbi request reprint Metabolism of benzo[a]pyrene to trans-7,8-dihydroxy-7, 8-dihydrobenzo[a]pyrene by recombinant human cytochrome P450 1B1 and purified liver epoxide hydrolase
    T Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537, Japan
    Chem Res Toxicol 12:623-9. 1999
  3. ncbi request reprint Prediction of human liver microsomal oxidations of 7-ethoxycoumarin and chlorzoxazone with kinetic parameters of recombinant cytochrome P-450 enzymes
    T Shimada
    Osaka Prefectural Institute of Public Health, Osaka, Japan
    Drug Metab Dispos 27:1274-80. 1999
  4. ncbi request reprint Roles of NADPH-P450 reductase in the O-deethylation of 7-ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli
    T Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka, 537 0025, Japan
    Protein Expr Purif 20:73-80. 2000
  5. ncbi request reprint Characterization of (+/-)-bufuralol hydroxylation activities in liver microsomes of Japanese and Caucasian subjects genotyped for CYP2D6
    T Shimada
    Osaka Prefectural Institute of Public Health, Japan
    Pharmacogenetics 11:143-56. 2001
  6. ncbi request reprint Specificity of 17beta-oestradiol and benzo[a]pyrene oxidation by polymorphic human cytochrome P4501B1 variants substituted at residues 48, 119 and 432
    T Shimada
    Osaka Prefectural Institute of Public Health, Japan
    Xenobiotica 31:163-76. 2001
  7. ncbi request reprint Metabolic activation of polycyclic aromatic hydrocarbons and other procarcinogens by cytochromes P450 1A1 and P450 1B1 allelic variants and other human cytochromes P450 in Salmonella typhimurium NM2009
    T Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Drug Metab Dispos 29:1176-82. 2001
  8. ncbi request reprint Inhibition of human cytochrome P450-catalyzed oxidations of xenobiotics and procarcinogens by synthetic organoselenium compounds
    T Shimada
    Osaka Prefectural Institute of Public Health, Japan
    Cancer Res 57:4757-64. 1997
  9. ncbi request reprint Tissue-specific induction of cytochromes P450 1A1 and 1B1 by polycyclic aromatic hydrocarbons and polychlorinated biphenyls in engineered C57BL/6J mice of arylhydrocarbon receptor gene
    Tsutomu Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Toxicol Appl Pharmacol 187:1-10. 2003
  10. ncbi request reprint Arylhydrocarbon receptor-dependent induction of liver and lung cytochromes P450 1A1, 1A2, and 1B1 by polycyclic aromatic hydrocarbons and polychlorinated biphenyls in genetically engineered C57BL/6J mice
    Tsutomu Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Carcinogenesis 23:1199-207. 2002

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Selectivity of polycyclic inhibitors for human cytochrome P450s 1A1, 1A2, and 1B1
    T Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Chem Res Toxicol 11:1048-56. 1998
    ....
  2. ncbi request reprint Metabolism of benzo[a]pyrene to trans-7,8-dihydroxy-7, 8-dihydrobenzo[a]pyrene by recombinant human cytochrome P450 1B1 and purified liver epoxide hydrolase
    T Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537, Japan
    Chem Res Toxicol 12:623-9. 1999
    ....
  3. ncbi request reprint Prediction of human liver microsomal oxidations of 7-ethoxycoumarin and chlorzoxazone with kinetic parameters of recombinant cytochrome P-450 enzymes
    T Shimada
    Osaka Prefectural Institute of Public Health, Osaka, Japan
    Drug Metab Dispos 27:1274-80. 1999
    ....
  4. ncbi request reprint Roles of NADPH-P450 reductase in the O-deethylation of 7-ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli
    T Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka, 537 0025, Japan
    Protein Expr Purif 20:73-80. 2000
    ....
  5. ncbi request reprint Characterization of (+/-)-bufuralol hydroxylation activities in liver microsomes of Japanese and Caucasian subjects genotyped for CYP2D6
    T Shimada
    Osaka Prefectural Institute of Public Health, Japan
    Pharmacogenetics 11:143-56. 2001
    ....
  6. ncbi request reprint Specificity of 17beta-oestradiol and benzo[a]pyrene oxidation by polymorphic human cytochrome P4501B1 variants substituted at residues 48, 119 and 432
    T Shimada
    Osaka Prefectural Institute of Public Health, Japan
    Xenobiotica 31:163-76. 2001
    ....
  7. ncbi request reprint Metabolic activation of polycyclic aromatic hydrocarbons and other procarcinogens by cytochromes P450 1A1 and P450 1B1 allelic variants and other human cytochromes P450 in Salmonella typhimurium NM2009
    T Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Drug Metab Dispos 29:1176-82. 2001
    ....
  8. ncbi request reprint Inhibition of human cytochrome P450-catalyzed oxidations of xenobiotics and procarcinogens by synthetic organoselenium compounds
    T Shimada
    Osaka Prefectural Institute of Public Health, Japan
    Cancer Res 57:4757-64. 1997
    ..These inhibitory actions may, in part, account for the mechanisms responsible for cancer prevention by organoselenium compounds in laboratory animals...
  9. ncbi request reprint Tissue-specific induction of cytochromes P450 1A1 and 1B1 by polycyclic aromatic hydrocarbons and polychlorinated biphenyls in engineered C57BL/6J mice of arylhydrocarbon receptor gene
    Tsutomu Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Toxicol Appl Pharmacol 187:1-10. 2003
    ....
  10. ncbi request reprint Arylhydrocarbon receptor-dependent induction of liver and lung cytochromes P450 1A1, 1A2, and 1B1 by polycyclic aromatic hydrocarbons and polychlorinated biphenyls in genetically engineered C57BL/6J mice
    Tsutomu Shimada
    Osaka Prefectural Institute of Public Health, 3 69 Nakamichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Carcinogenesis 23:1199-207. 2002
    ....
  11. ncbi request reprint Electron transport pathway for a Streptomyces cytochrome P450: cytochrome P450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2)
    Young Jin Chun
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 282:17486-500. 2007
    ..Thus, an electron transfer pathway to a functional Streptomyces P450 has been established...
  12. ncbi request reprint Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b(5) enzymes
    Tsutomu Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 638 Robinson Research Building, 23rd and Pierce Avenues, Nashville, TN 37232 0146, USA
    Arch Biochem Biophys 435:207-16. 2005
    ....
  13. ncbi request reprint Roles of human CYP2A6 and 2B6 and rat CYP2C11 and 2B1 in the 10-hydroxylation of (-)-verbenone by liver microsomes
    Mitsuo Miyazawa
    Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Kowakae, Higashiosaka, Osaka, Japan
    Drug Metab Dispos 31:1049-53. 2003
    ....
  14. ncbi request reprint Inhibition of human cytochrome P450 1A1-, 1A2-, and 1B1-mediated activation of procarcinogens to genotoxic metabolites by polycyclic aromatic hydrocarbons
    Tsutomu Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 606 Light Hall, 2215 Garland Avenue, Nashville, Tennessee 37232 0146, USA
    Chem Res Toxicol 19:288-94. 2006
    ....
  15. pmc Oxidation of pyrene, 1-hydroxypyrene, 1-nitropyrene and 1-acetylpyrene by human cytochrome P450 2A13
    Tsutomu Shimada
    a Laboratory of Cellular and Molecular Biology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan
    Xenobiotica 46:211-24. 2016
    ..5. These results suggest that P450 2A13 is one of the important enzymes that oxidizes these PAH compounds and may determine how these chemicals are detoxicated and bioactivated in humans...
  16. ncbi request reprint Cytochrome P450 1B1: a target for inhibition in anticarcinogenesis strategies
    F Peter Guengerich
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 638 Robinson Research Building, 23rd Pierce Avenue, Nashville, TN 37232 0146, USA
    Mutat Res 523:173-82. 2003
    ..Substituted stilbenes may be useful in preventing cancer caused by estrogens and xenobiotics...
  17. ncbi request reprint Activation of aminophenylnorharman, aminomethylphenylnorharman and aminophenylharman to genotoxic metabolites by human N-acetyltransferases and cytochrome P450 enzymes expressed in Salmonella typhimurium umu tester strains
    Yoshimitsu Oda
    Osaka Prefectural Institute of Public Health, 3 69 Nanakichi 1 chome, Higashinari ku, Osaka 537 0025, Japan
    Mutagenesis 21:411-6. 2006
    ..From these results, it is concluded that APNH, AMPNH and APH are mainly bioactivated by P450 1A2 and NAT2, followed by NAT1 enzymes. P450 1A1 was also found to activate AMPNH at relatively slower rates...
  18. pmc Metabolic activation of polycyclic aromatic hydrocarbons and aryl and heterocyclic amines by human cytochromes P450 2A13 and 2A6
    Tsutomu Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, United States
    Chem Res Toxicol 26:529-37. 2013
    ..These results suggest that P450 2A enzymes, as well as P450 Family 1 enzymes including P450 1B1, are major enzymes involved in activating PAHs and aryl- and heterocyclic amines, as well as tobacco-related nitrosamines. ..
  19. ncbi request reprint Roles of Human CYP2A6 and Monkey CYP2A24 and 2A26 Cytochrome P450 Enzymes in the Oxidation of 2,5,2',5'-Tetrachlorobiphenyl
    Tsutomu Shimada
    Laboratory of Cellular and Molecular Biology, Osaka Prefecture University, Izumisano, Osaka, Japan T S, S T, M K Osaka Prefectural Institute of Public Health, Higashinari ku, Osaka, Japan K K Faculty of Nutritional Sciences, Nakamura Gakuen University, Johnan ku, Fukuoka, Japan N K Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo, Japan S U, N M, H Y Department of Biological Sciences, Konkuk University, Seoul, South Korea D K and Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee F P G
    Drug Metab Dispos 44:1899-1909. 2016
    ..e., V110L, I209S, I300F, V365M, S369G, and R372H, based on the comparison of primary sequences...
  20. ncbi request reprint Recombinant enzymes overexpressed in bacteria show broad catalytic specificity of human cytochrome P450 2W1 and limited activity of human cytochrome P450 2S1
    Zhong Liu Wu
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 638 Robinson Research Building, 23rd and Pierce Avenues, Nashville, TN 37232 0146, USA
    Mol Pharmacol 69:2007-14. 2006
    ....
  21. ncbi request reprint Metabolism of (+)- and (-)-limonenes to respective carveols and perillyl alcohols by CYP2C9 and CYP2C19 in human liver microsomes
    Mitsuo Miyazawa
    Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Kowakae, Higashiosaka, Osaka, Japan
    Drug Metab Dispos 30:602-7. 2002
    ..Species-related differences exist in the oxidations of limonenes in CYP2B subfamily in rats and humans...
  22. ncbi request reprint Xenobiotic-metabolizing enzymes involved in activation and detoxification of carcinogenic polycyclic aromatic hydrocarbons
    Tsutomu Shimada
    Department of Chemical Biology, Osaka City University Medical School, Osaka, Japan
    Drug Metab Pharmacokinet 21:257-76. 2006
    ..Factors affecting such variations include induction and inhibition of enzymes by diverse chemicals and, more importantly, genetic polymorphisms of enzymes in humans...
  23. ncbi request reprint Use of heterologously-expressed cytochrome P450 and glutathione transferase enzymes in toxicity assays
    F Peter Guengerich
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 638 Robinson Research Building, Medical Research Building I, Nashville, TN 37232 0146, USA
    Toxicology 181:261-4. 2002
    ..These systems have been used to compare these GSTs with regard to activation of dihaloalkanes and potential toxicity...
  24. ncbi request reprint Metabolic activation of polycyclic aromatic hydrocarbons to carcinogens by cytochromes P450 1A1 and 1B1
    Tsutomu Shimada
    Osaka Prefectural Institute of Public Health, Higashinari ku, Osaka 537 0025, Japan
    Cancer Sci 95:1-6. 2004
    ..Differences in the susceptibility of individuals to the adverse action of PAHs may, in part, be due to differences in the levels of expression of CYP1A1 and 1B1 and to genetic variations in the CYP1A1 and 1B1 genes...
  25. ncbi request reprint Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2
    Bin Zhao
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 280:11599-607. 2005
    ..The flaviolin molecules form a quasi-planar three-molecule stack including the heme of CYP158A2, suggesting that oxidative C-C coupling of these phenolic molecules leads to the production of flaviolin dimers...
  26. pmc Binding of diverse environmental chemicals with human cytochromes P450 2A13, 2A6, and 1B1 and enzyme inhibition
    Tsutomu Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, United States
    Chem Res Toxicol 26:517-28. 2013
    ..These results indicate that P450 2A13, as well as Family 1 P450 enzymes, is able to catalyze many detoxication and activation reactions with chemicals of environmental interest...
  27. ncbi request reprint Cytochrome P450 reconstitution systems
    Hiroshi Yamazaki
    Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo, Japan
    Methods Mol Biol 320:61-71. 2006
    ..In this chapter, we describe optimal conditions that have been determined in our laboratories for the reconstitution of drug oxidation activities catalyzed by purified human CYP1A2, 2C9, 2E1, and 3A4...
  28. ncbi request reprint Sex differences in the metabolism of (+)- and (-)-limonene enantiomers to carveol and perillyl alcohol derivatives by cytochrome p450 enzymes in rat liver microsomes
    Mitsuo Miyazawa
    Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Kowakae, Higashiosaka, Osaka 577 8502, Japan
    Chem Res Toxicol 15:15-20. 2002
    ....
  29. ncbi request reprint Roles of NADPH-P450 reductase and apo- and holo-cytochrome b5 on xenobiotic oxidations catalyzed by 12 recombinant human cytochrome P450s expressed in membranes of Escherichia coli
    Hiroshi Yamazaki
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    Protein Expr Purif 24:329-37. 2002
    ..P450/NPR membrane systems containing b5 are useful models for prediction of the rates for liver microsomal P450-dependent drug oxidations...
  30. doi request reprint Mechanism of decrease of oral bioavailability of cyclosporin A during immunotherapy upon coadministration of amphotericin B
    Junko Ishizaki
    Department of Clinical Pharmaceutics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Japan
    Biopharm Drug Dispos 29:195-203. 2008
    ..It is suggested that careful monitoring of CyA levels is necessary in the event of AMB administration to patients receiving immunotherapy with CyA...
  31. ncbi request reprint Modulation of mdr1a and CYP3A gene expression in the intestine and liver as possible cause of changes in the cyclosporin A disposition kinetics by dexamethasone
    Koichi Yokogawa
    Department of Hospital Pharmacy, School of Medicine, Kanazawa University, Kanazawa, Japan
    Biochem Pharmacol 63:777-83. 2002
    ..Our results indicate that the drug interaction between CyA and DEX is a consequence of modulation of P-gp and CYP3A2 gene expression by DEX, with differential dose-dependence...
  32. ncbi request reprint Different mechanisms for inhibition of human cytochromes P450 1A1, 1A2, and 1B1 by polycyclic aromatic inhibitors
    Tsutomu Shimada
    Department of Chemical Biology, Osaka City University Medical School, Abeno Ku, Osaka 545 8585, Japan
    Chem Res Toxicol 20:489-96. 2007
    ..These results suggest different mechanisms of inhibition of P450 1A1, 1A2, and 1B1 by PAHs and related chemicals and that interactions between P450 enzymes and PAH inhibitors are involved in differences in inhibition of the enzymes...
  33. ncbi request reprint Site-dependent contributions of P-glycoprotein and CYP3A to cyclosporin A absorption, and effect of dexamethasone in small intestine of mice
    Mingji Jin
    Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920 1192, Japan
    Biochem Pharmacol 72:1042-50. 2006
    ....
  34. ncbi request reprint Influence of cytarabine and cyclophosphamide on the disposition kinetics of cyclosporin A after bone marrow transplantation
    Tsutomu Shimada
    Hospital Pharmacy, School of Medicine, Kanazawa University, Takara machi 13 1, 920 8641, Kanazawa, Japan
    Transpl Int 16:788-93. 2003
    ....
  35. ncbi request reprint Contributions of intestinal P-glycoprotein and CYP3A to oral bioavailability of cyclosporin A in mice treated with or without dexamethasone
    Mingji Jin
    Department of Clinical Pharmacy, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920 1192, Japan
    Int J Pharm 309:81-6. 2006
    ....
  36. ncbi request reprint Long-term levothyroxine treatment decreases the oral bioavailability of cyclosporin A by inducing P-glycoprotein in small intestine
    Mingji Jin
    Department of Clinical Pharmacy, Graduate School of Natural Science and Technology, Kanazawa University, Takara machi, Japan
    Drug Metab Pharmacokinet 20:324-30. 2005
    ..In conclusion, careful monitoring of CyA levels is required in the event of LTX administration to patients receiving immunotherapy with CyA...
  37. ncbi request reprint Decreased coumarin 7-hydroxylase activities and CYP2A6 expression levels in humans caused by genetic polymorphism in CYP2A6 promoter region (CYP2A6*9)
    Kazuma Kiyotani
    Laboratory of Drug Metabolism, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Pharmacogenetics 13:689-95. 2003
    ..Individuals judged as CYP2A6*4/*9 were expected to be poor metabolizers, having extremely low activity of CYP2A6...
  38. ncbi request reprint Effects of 1-benzylxanthines on cyclic AMP phosphodiesterase 4 isoenzyme
    Hirokazu Suzuki
    Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan
    Biol Pharm Bull 29:131-4. 2006
    ..3,7-Dihydro-7-acetonyl-1-(2,4-dichlorobenzyl)-3-propyl-1H-purine-2,4-dione (2h) exhibited both more selective and more potent PDE4 inhibitory activity than rolipram and XT-611...
  39. ncbi request reprint Obesity-induced increase of CYP2E1 activity and its effect on disposition kinetics of chlorzoxazone in Zucker rats
    Phisit Khemawoot
    Department of Medicinal Informatics, Division of Cardiovascular Medicine, Graduate School of Medical Science, Kanazawa University, Japan
    Biochem Pharmacol 73:155-62. 2007
    ..These results suggest that CYP2E1 may be induced in liver and fat of obese patients, thereby potentially altering the disposition kinetics of not only CZX, but also other lipophilic drugs metabolized by CYP2E1...
  40. ncbi request reprint Lowered blood concentration of tacrolimus and its recovery with changes in expression of CYP3A and P-glycoprotein after high-dose steroid therapy
    Tsutomu Shimada
    Department of Hospital Pharmacy, School of Medicine, Kanazawa University, Kanazawa, Japan
    Transplantation 74:1419-24. 2002
    ..We conducted a study in rats to clarify the mechanism of the changes in the blood concentration of FK506 during and after steroid therapy...
  41. ncbi request reprint [Effect of intraperitoneal chemotherapy on experimental peritoneal dissemination of gastric cancer]
    Yutaka Yonemura
    Peritoneal Dissemination Program, Shizuoka Cancer Center, Kanazawa University Hospital
    Gan To Kagaku Ryoho 32:1635-9. 2005
    ..These results suggest the potential of po TS-1 + ip CDDP, ip 5-FU, ip docetaxel and ip CBDCA administration for the treatment of peritoneal dissemination of gastric cancer...
  42. doi request reprint Monosodium glutamate (MSG): a villain and promoter of liver inflammation and dysplasia
    Yuko Nakanishi
    Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
    J Autoimmun 30:42-50. 2008
    ..These results take on considerable significance in light of the widespread usage of dietary MSG and we suggest that MSG should have its safety profile re-examined and be potentially withdrawn from the food chain...
  43. ncbi request reprint Pharmacokinetic advantage of intraperitoneal injection of docetaxel in the treatment for peritoneal dissemination of cancer in mice
    Tsutomu Shimada
    Department of Hospital Pharmacy, Kanazawa University School of Medicine, 13 1 Takara machi, Kanazawa 920 8641, Japan
    J Pharm Pharmacol 57:177-81. 2005
    ..These results indicated that the intraperitoneal administration of docetaxel for peritoneal dissemination was likely to be an effective treatment method, without causing any increase in systemic toxicity...
  44. ncbi request reprint Molecular modelling of human CYP1B1 substrate interactions and investigation of allelic variant effects on metabolism
    David F V Lewis
    School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Chem Biol Interact 145:281-95. 2003
    ....
  45. ncbi request reprint High-performance liquid chromatographic method for the determination of (-)-verbenone 10-hydroxylation catalyzed by rat liver microsomes
    Mitsuo Miyazawa
    Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Kowakae, Higashiosaka, Osaka 577 8502, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 793:291-6. 2003
    ..8 and 44 nmol/min/mg protein, respectively. Thus the present results provided a sensitive and useful method for the determination of verbenone 10-hydroxylation catalyzed by rat liver microsomes...