R Masui

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. pmc The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8
    Hiromasa Ohyama
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    BMC Genomics 15:386. 2014
  2. doi request reprint Structural insights of post-translational modification sites in the proteome of Thermus thermophilus
    Ryoji Masui
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka, 560 0043, Japan
    J Struct Funct Genomics 15:137-51. 2014
  3. pmc Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity
    Shuhei Nakane
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 37:2037-52. 2009
  4. pmc Nuclease activity of the MutS homologue MutS2 from Thermus thermophilus is confined to the Smr domain
    Kenji Fukui
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 35:850-60. 2007
  5. pmc Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry
    Kenji Fukui
    RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo cho, Sayo gun, Hyogo 679 5148, Japan
    Nucleic Acids Res 35:e100. 2007
  6. pmc A commensal symbiotic interrelationship for the growth of Symbiobacterium toebii with its partner bacterium, Geobacillus toebii
    Kwang Kim
    Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka 560 0043, Japan
    BMC Res Notes 4:437. 2011
  7. ncbi request reprint Biochemical characterization of TT1383 from Thermus thermophilus identifies a novel dNTP triphosphohydrolase activity stimulated by dATP and dTTP
    Naoyuki Kondo
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka
    J Biochem 136:221-31. 2004
  8. pmc Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain
    A Yamagata
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 29:4617-24. 2001
  9. ncbi request reprint Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair
    N Nakagawa
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560 0043, Japan
    J Biochem 126:986-90. 1999
  10. ncbi request reprint The RadA protein from a hyperthermophilic archaeon Pyrobaculum islandicum is a DNA-dependent ATPase that exhibits two disparate catalytic modes, with a transition temperature at 75 degrees C
    M Spies
    Department of Biology, Graduate School of Science, Osaka University, Japan
    Eur J Biochem 267:1125-37. 2000

Collaborators

Detail Information

Publications50

  1. pmc The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8
    Hiromasa Ohyama
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    BMC Genomics 15:386. 2014
    ....
  2. doi request reprint Structural insights of post-translational modification sites in the proteome of Thermus thermophilus
    Ryoji Masui
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka, 560 0043, Japan
    J Struct Funct Genomics 15:137-51. 2014
    ..Our findings also indicate that phosphorylation of a residue occurs more frequently at a flexible region of the protein, whereas lysine acetylation occurs more frequently in an ordered structure...
  3. pmc Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity
    Shuhei Nakane
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 37:2037-52. 2009
    ..Our findings provide a molecular insight into the functional domain organization of bacterial PolXs, especially the requirement of the PHP domain for 3'-5' exonuclease activity...
  4. pmc Nuclease activity of the MutS homologue MutS2 from Thermus thermophilus is confined to the Smr domain
    Kenji Fukui
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 35:850-60. 2007
    ..Furthermore, the Smr domain alone was stable and functional in binding and incising DNA. It is noteworthy that an endonuclease activity is associated with a MutS homologue, which is generally thought to recognize specific DNA structures...
  5. pmc Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry
    Kenji Fukui
    RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo cho, Sayo gun, Hyogo 679 5148, Japan
    Nucleic Acids Res 35:e100. 2007
    ..The simultaneous identification of the innumerable fragments was achieved by the extremely high-accuracy of ESI-FT ICR MS...
  6. pmc A commensal symbiotic interrelationship for the growth of Symbiobacterium toebii with its partner bacterium, Geobacillus toebii
    Kwang Kim
    Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka 560 0043, Japan
    BMC Res Notes 4:437. 2011
    ..abstract:..
  7. ncbi request reprint Biochemical characterization of TT1383 from Thermus thermophilus identifies a novel dNTP triphosphohydrolase activity stimulated by dATP and dTTP
    Naoyuki Kondo
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka
    J Biochem 136:221-31. 2004
    ..This is, to our knowledge, the first report of a non-specific dNTPase that is activated by dNTP itself...
  8. pmc Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain
    A Yamagata
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 29:4617-24. 2001
    ..Both proteins have 5'-->3' exonuclease activity, which was enhanced by increasing the temperature to 50 degrees C. Mg(2+), Mn(2+) or Co(2+) ions were required to activate both proteins, whereas Ca(2+) and Zn(2+) had no effects...
  9. ncbi request reprint Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair
    N Nakagawa
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560 0043, Japan
    J Biochem 126:986-90. 1999
    ..The sequence similarity suggests that the beta-domain interacts with UvrA. Based on the distribution of the conserved regions and the structure of the PcrA-DNA complex, a model for the UvrB-DNA complex is proposed...
  10. ncbi request reprint The RadA protein from a hyperthermophilic archaeon Pyrobaculum islandicum is a DNA-dependent ATPase that exhibits two disparate catalytic modes, with a transition temperature at 75 degrees C
    M Spies
    Department of Biology, Graduate School of Science, Osaka University, Japan
    Eur J Biochem 267:1125-37. 2000
    ..These results suggest that the ssDNA-dependent ATPase activity of P. islandicum RadA displays a temperature-dependent capacity to exist in two different catalytic modes, with 75 degrees C being the critical threshold temperature...
  11. ncbi request reprint The Nudix hydrolase Ndx1 from Thermus thermophilus HB8 is a diadenosine hexaphosphate hydrolase with a novel activity
    Takayoshi Iwai
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043
    J Biol Chem 279:21732-9. 2004
    ..Trp-26 was likely involved in enzyme-substrate interactions based on fluorescence measurements. Based on these results, the mechanism of substrate recognition and catalysis are discussed...
  12. ncbi request reprint Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8
    A Shibata
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Acta Crystallogr D Biol Crystallogr 55:704-5. 1999
    ..8 A3 Da-1. The crystals diffracted X-rays beyond 2.9 A resolution. Although the crystals were sensitive to X-ray irradiation at room temperature, the frozen crystals at 100 K showed no apparent decay during the intensity measurement...
  13. pmc Domain organization and functional analysis of Thermus thermophilus MutS protein
    H Tachiki
    Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    Nucleic Acids Res 26:4153-9. 1998
    ..Among the proteolytic fragments, the B domain bound to dsDNA. On the basis of these results we have proposed the domain organization of T. thermophilus MutS and putative roles of these domains...
  14. pmc Crystal structure of thermostable DNA photolyase: pyrimidine-dimer recognition mechanism
    H Komori
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 98:13560-5. 2001
    ..This structural data for the photolyase-thymine complex allow us to propose a detailed model for the pyrimidine-dimer recognition mechanism...
  15. ncbi request reprint Structural insights into the Thermus thermophilus ADP-ribose pyrophosphatase mechanism via crystal structures with the bound substrate and metal
    Sachiko Yoshiba
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biol Chem 279:37163-74. 2004
    ..This water molecule is situated at an ideal position to carry out nucleophilic attack on the adenosyl phosphate, as it is 3.6 A away from the target phosphorus and almost in line with the scissile bond...
  16. ncbi request reprint [Structural proteomics of metabolism-related proteins]
    Seiki Kuramitsu
    Tanpakushitsu Kakusan Koso 53:604-7. 2008
  17. ncbi request reprint Molecular mechanism of the Thermus thermophilus ADP-ribose pyrophosphatase from mutational and kinetic studies
    Takushi Ooga
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Biochemistry 44:9320-9. 2005
    ..Our results highlight the diversity within the nudix protein family in terms of substrate recognition and catalysis...
  18. ncbi request reprint An aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8
    A Okamoto
    Department of Biology, Faculty of Science, Osaka University, Toyonaka
    J Biochem 119:135-44. 1996
    ..Despite the high degree of conservation of active-site residues, Arg292 in E. coli AspAT, which interacts with the distal carboxylate of the substrate, was not found in T. thermophilus AspAT. Arg89 may complement the function of Arg292...
  19. ncbi request reprint Structure of dNTP-inducible dNTP triphosphohydrolase: insight into broad specificity for dNTPs and triphosphohydrolase-type hydrolysis
    Naoyuki Kondo
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    Acta Crystallogr D Biol Crystallogr 63:230-9. 2007
    ..Recognition of the triphosphate moiety crucial for the activity might be attained by highly conserved positively charged residues. The possible mode of dNTP binding is discussed in light of the structure...
  20. ncbi request reprint Crystal structure of family 5 uracil-DNA glycosylase bound to DNA
    Hiromichi Kosaka
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    J Mol Biol 373:839-50. 2007
    ..Based on no significant open-closed conformational change upon binding to DNA, we propose a "slide-in" mechanism for initial damage recognition...
  21. ncbi request reprint Rapid and sensitive amino-acid sequencing of cloning Thermus thermophilus HB8 ferredoxin by proteomics
    Maki Kaneko
    Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043 Japan
    J Proteome Res 3:983-7. 2004
    ..The catalytic electron-transfer reactivity of the [7Fe-8S] ferredoxin between ferredoxin-NADP+ reductase and cytochrome c was recognized...
  22. pmc The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity
    Atsushi Yamagata
    Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka 560 0043, Japan
    Proc Natl Acad Sci U S A 99:5908-12. 2002
    ..The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA...
  23. ncbi request reprint Thermus thermophilus MutS2, a MutS paralogue, possesses an endonuclease activity promoted by MutL
    Kenji Fukui
    Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyamacho, Toyonaka, Osaka 560 0043
    J Biochem 135:375-84. 2004
    ..ttMutS2 incises not only oligo dsDNA but also plasmid DNA, suggesting that ttMutS2 possesses an endonuclease activity. At low concentrations, the incision activity was not retained, but was promoted by T. thermophilus MutL...
  24. ncbi request reprint Crystallization and preliminary X-ray diffraction studies of nucleoside diphosphate kinase from Thermus thermophilus HB8
    Shunsaku Takeishi
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Acta Crystallogr D Biol Crystallogr 59:1843-5. 2003
    ..7 A(3) Da(-1). The crystals diffracted X-rays generated by the synchrotron at SPring-8 to at least 1.9 A resolution and were suitable for high-resolution X-ray crystal structure determination...
  25. ncbi request reprint Crystal structure of TTHA0252 from Thermus thermophilus HB8, a RNA degradation protein of the metallo-beta-lactamase superfamily
    Hirohito Ishikawa
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biochem 140:535-42. 2006
    ..We also detected nuclease activity of TTHA0252 against rRNAs of T. thermophilus. Our results reveal structural and functional aspects of novel RNase E-like enzymes with a beta-CASP fold...
  26. ncbi request reprint Overproduction, crystallization and preliminary diffraction data of ADP-ribose pyrophosphatase from Thermus thermophilus HB8
    Sachiko Yoshiba
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Acta Crystallogr D Biol Crystallogr 59:1840-2. 2003
    ..50 A resolution from the apo form using synchrotron radiation and to 2.0 A resolution from the complexed form. Both crystals belong to space group P3(1)21 or P3(2)21 and contain one molecule in the asymmetric unit...
  27. pmc A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways
    Atsuhiro Shimada
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043 and RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo cho, Sayo gun, Hyogo 679 5148, Japan
    Nucleic Acids Res 38:5692-705. 2010
    ..Consequently, we concluded that TTHB178 is a novel 3'-5' ssExo that functions in various DNA repair systems in cooperation with or independently of RecJ. We named TTHB178 as T. thermophilus exonuclease I...
  28. pmc Degradation of ppGpp by nudix pyrophosphatase modulates the transition of growth phase in the bacterium Thermus thermophilus
    Takushi Ooga
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biol Chem 284:15549-56. 2009
    ..These results suggest a novel mechanism of ppGpp-mediated growth control by the functional relay between Ndx8 and SpoT activity as ppGpp scavengers...
  29. doi request reprint An O6-methylguanine-DNA methyltransferase-like protein from Thermus thermophilus interacts with a nucleotide excision repair protein
    Rihito Morita
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    J Biochem 144:267-77. 2008
    ..Interaction of TTHA1564 with UvrA was confirmed using a surface plasmon resonance assay. These results suggest the possible involvement of TTHA1564 in DNA repair pathways...
  30. ncbi request reprint Novel reaction mechanism of GTP cyclohydrolase I. High-resolution X-ray crystallography of Thermus thermophilus HB8 enzyme complexed with a transition state analogue, the 8-oxoguanine derivative
    Yoko Tanaka
    Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    J Biochem 138:263-75. 2005
    ..Superimpositioning of the structures indicates the imidazole ring of His110 is rotated, implying concomitant proton transfer to the ribose ring O4'. Based on these structural data we propose a novel reaction mechanism for GTPCH1...
  31. ncbi request reprint Improved heterologous gene expression in Escherichia coli by optimization of the AT-content of codons immediately downstream of the initiation codon
    Takashi Nishikubo
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biotechnol 120:341-6. 2005
    ..This result suggests that incorporation of AT-rich codons can be utilized as a general strategy for improving the expression efficiency of a recombinant protein...
  32. ncbi request reprint Overproduction, crystallization and preliminary X-ray diffraction analysis of a quinone oxidoreductase from Thermus thermophilus HB8
    Yoshimitsu Shimomura
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Acta Crystallogr D Biol Crystallogr 58:1365-7. 2002
    ..The asymmetric unit has one protein subunit (V(M) = 3.2 A(3) Da(-1) and V(sol) = 0.62 for the apo form), indicating that the twofold axis of the dimeric protein and the crystallographic twofold axis coincide...
  33. ncbi request reprint Simultaneous detection of N-terminal fragment ions in a protein mixture using a ruthenium(II) complex
    Akihiro Ito
    Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Rapid Commun Mass Spectrom 21:2647-53. 2007
    ..Application to unknown proteins from Thermus thermophilus HB8 with two-dimensional electrophoretic separation resulted in the successful determination of the N-terminal sequence and easy identification of the target protein...
  34. ncbi request reprint Insights into different dependence of dNTP triphosphohydrolase on metal ion species from intracellular ion concentrations in Thermus thermophilus
    Naoyuki Kondo
    Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama cho, Toyonaka, Osaka 560 0043, Japan
    Extremophiles 12:217-23. 2008
    ..In addition, the proposed model of dNTPase activity induced by magnesium and multiple dNTPs was discussed based on the results obtained in this study...
  35. ncbi request reprint Crystal structure of the native chaperonin complex from Thermus thermophilus revealed unexpected asymmetry at the cis-cavity
    Tatsuro Shimamura
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    Structure 12:1471-80. 2004
    ..As a result, the GroEL-GroES interface differs considerably from the previously reported E. coli GroEL-GroES complex, including a previously unknown contact between GroEL and GroES...
  36. ncbi request reprint Crystal structure of a family 4 uracil-DNA glycosylase from Thermus thermophilus HB8
    Jun Hoseki
    RIKEN Harima Institute at SPring 8, 1 1 1 Kouto, Mikazuki, Sayo gun, Hyogo 679 5148, Japan
    J Mol Biol 333:515-26. 2003
    ..coli MUG (family 2). These results suggest that the mechanism by which family 4 UDGs remove uracils from DNA is similar to that of family 1 enzymes...
  37. pmc Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain
    Akio Ebihara
    RIKEN SPring 8 Center, Harima Institute, Hyogo 679 5148, Japan
    Protein Sci 15:1494-9. 2006
    ..This report shows that TTHB192 and its sequence homologs adopt an RNA recognition motif-like domain and provides the first testable functional hypothesis for this protein family...
  38. doi request reprint Bound nucleotide controls the endonuclease activity of mismatch repair enzyme MutL
    Kenji Fukui
    RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo cho, Sayo gun, Hyogo 679 5148, Japan
    J Biol Chem 283:12136-45. 2008
    ..Complementation experiments revealed that the endonuclease activity of ttMutL and its regulation by ATP binding are necessary for DNA repair in vivo...
  39. ncbi request reprint Ligand-induced conformational changes and a reaction intermediate in branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8, as revealed by X-ray crystallography
    Tadashi Nakai
    RIKEN Harima Institute SPring 8, 1 1 1 Kouto, Mikazuki, Sayo gun, Hyogo 679 5148, Japan
    J Mol Biol 337:1011-33. 2004
    ..MOPA and of E1(ceim).lipoamide built from the above structures suggest that His273alpha and His129beta' are potential proton donors to the carbonyl group of a BCOA and to the proximal sulfur atom on the lipoamide, respectively...
  40. ncbi request reprint A novel metal-activated L-serine O-acetyltransferase from Thermus thermophilus HB8
    Shin ichiro Kobayashi
    RIKEN Harima Institute SPring 8, Hyogo 679 5148, Japan
    J Biochem 136:629-34. 2004
    ..These results indicate that the T. thermophilus SAT is a novel type of enzyme different from other members of this protein family...
  41. ncbi request reprint Structure of Thermus thermophilus HB8 H-protein of the glycine-cleavage system, resolved by a six-dimensional molecular-replacement method
    Tadashi Nakai
    RIKEN Harima Institute SPring 8, 1 1 1 Kouto, Mikazuki, Sayo gun, Hyogo 679 5148, Japan
    Acta Crystallogr D Biol Crystallogr 59:1610-8. 2003
    ..Meanwhile, the lipoyl-lysine arm of the T. thermophilus H-protein was found to be more flexible than that of the pea H-protein, supporting the hypothesis that H-protein does not form a stable complex with L-protein during the reaction...
  42. pmc Structure of P-protein of the glycine cleavage system: implications for nonketotic hyperglycinemia
    Tadashi Nakai
    RIKEN Harima Institute at SPring 8, Mikazuki, Sayo, Hyogo, Japan
    EMBO J 24:1523-36. 2005
    ..These results provide insights into the molecular basis of nonketotic hyperglycinemia...
  43. ncbi request reprint Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate
    Seiko Yoshikawa
    Protein Research Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama 230 0045, Japan
    Acta Crystallogr D Biol Crystallogr 63:357-65. 2007
    ..horikoshii glyoxylate reductase contains aromatic clusters and has a larger number of ion pairs and a lower percentage of hydrophobic accessible surface area than its mesophilic homologues, suggesting its thermostability mechanism...
  44. ncbi request reprint Fluorescence resonance energy transfer analysis of protein translocase. SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes
    Hiroyuki Mori
    Institute for Virus Research, Kyoto University, Japan
    J Biol Chem 278:14257-64. 2003
    ....
  45. ncbi request reprint Coexpression, purification, crystallization and preliminary X-ray characterization of glycine decarboxylase (P-protein) of the glycine-cleavage system from Thermus thermophilus HB8
    Tadashi Nakai
    RIKEN Harima Institute SPring 8, 1 1 1 Kouto, Mikazuki, Sayo gun, Hyogo 679 5148, Japan
    Acta Crystallogr D Biol Crystallogr 59:554-7. 2003
    ..15 A(3) Da(-1)) and self-rotation function analysis suggest the presence of one alpha(2)beta(2) tetramer per asymmetric unit, with the molecules related by non-crystallographic twofold symmetry...
  46. ncbi request reprint Structure-based functional identification of a novel heme-binding protein from Thermus thermophilus HB8
    Akio Ebihara
    RIKEN Harima Institute at SPring 8, Kouto, Mikazuki cho, Sayo gun, Hyogo, 679 5148, Japan
    J Struct Funct Genomics 6:21-32. 2005
    ..The presence of a possible Fe-His-Asp triad in the heme proximal site suggests that TT1485 functions as a novel heme peroxidase to detoxify hydrogen peroxide within the cell...
  47. ncbi request reprint Multidrug transporter MexB of Pseudomonas aeruginosa: overexpression, purification, and initial structural characterization
    Vladislav Mokhonov
    Department of Molecular Life Science, Tokai University, School of Medicine, 143 Shimokasuya, Isehara 259 1193, Japan
    Protein Expr Purif 40:91-100. 2005
    ..Circular dichroism analysis of MexB showed dominance of the alpha-helix structures. High yield, homogeneity, and stability of MexB position it as a good candidate for structural and functional characterization...
  48. ncbi request reprint [An exhaustive overproduction of bacterial proteins]
    Naoto Ohtani
    Tanpakushitsu Kakusan Koso 47:1009-13. 2002
  49. ncbi request reprint Trigger factor from Thermus thermophilus is a Zn2+-dependent chaperone
    Ryoji Suno
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226 8503, Japan
    J Biol Chem 279:6380-4. 2004
    ..5 mol Zn2+/mol TF. The folding-arrest activity of TF that was saturated with Zn2+ (approximately 1 mol/mol TF) was twice as efficient as that of untreated TF. Thus, chaperone activity of thermophilic TF is Zn2+-dependent...
  50. pmc Structural basis for different substrate specificities of two ADP-ribose pyrophosphatases from Thermus thermophilus HB8
    Taisuke Wakamatsu
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    J Bacteriol 190:1108-17. 2008
    ..These results suggest that ADPRase-I and ADPRase-II enzymes have nearly identical catalytic mechanisms but different mechanisms of substrate recognition...