Tetsuya Iida

Summary

Affiliation: Osaka University
Country: Japan

Publications

  1. pmc Metagenomic profile of gut microbiota in children during cholera and recovery
    Shirajum Monira
    International Center for Diarrhoeal Disease Research, Bangladesh
    Gut Pathog 5:1. 2013
  2. pmc Genomic taxonomy of Vibrios
    Cristiane C Thompson
    Laboratory of Molecular Genetics of Microrganims, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
    BMC Evol Biol 9:258. 2009
  3. ncbi request reprint Close proximity of the tdh, trh and ure genes on the chromosome of Vibrio parahaemolyticus
    T Iida
    Department of Bacterial Infections, Osaka University, Japan
    Microbiology 144:2517-23. 1998
  4. pmc Filamentous phage associated with recent pandemic strains of Vibrio parahaemolyticus
    T Iida
    Osaka University, Suita, Osaka, Japan
    Emerg Infect Dis 7:477-8. 2001
  5. ncbi request reprint [Type III secretion systems and bacterial pathogenicity]
    Tetsuya Iida
    Tanpakushitsu Kakusan Koso 50:29-35. 2005
  6. pmc Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome
    Tetsuya Iida
    Department of Bacterial Infections Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Bacteriol 184:4933-5. 2002
  7. pmc Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2
    Toshio Kodama
    Department of Bacterial Infections, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Infect Immun 76:4282-9. 2008
  8. pmc Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region
    Toshio Kodama
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    PLoS ONE 5:e8678. 2010
  9. doi request reprint VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity
    Hirotaka Hiyoshi
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Cell Host Microbe 10:401-9. 2011
  10. ncbi request reprint Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors: Tir and EspFu/TccP
    Vlademir V Cantarelli
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Microbiol 9:1782-95. 2007

Detail Information

Publications81

  1. pmc Metagenomic profile of gut microbiota in children during cholera and recovery
    Shirajum Monira
    International Center for Diarrhoeal Disease Research, Bangladesh
    Gut Pathog 5:1. 2013
    ..The diverse bacterial communities colonizing the gut (gastrointestinal tract) of infants as commensal flora, which play an important role in nutrient absorption and determining the state of health, are known to alter due to diarrhea...
  2. pmc Genomic taxonomy of Vibrios
    Cristiane C Thompson
    Laboratory of Molecular Genetics of Microrganims, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
    BMC Evol Biol 9:258. 2009
    ..Our aim is to analyse the usefulness of these tools for species identification in vibrios...
  3. ncbi request reprint Close proximity of the tdh, trh and ure genes on the chromosome of Vibrio parahaemolyticus
    T Iida
    Department of Bacterial Infections, Osaka University, Japan
    Microbiology 144:2517-23. 1998
    ..5 kb. These results reveal a close proximity of the tdh, trh and ure genes on the chromosome of pathogenic V. parahaemolyticus strains...
  4. pmc Filamentous phage associated with recent pandemic strains of Vibrio parahaemolyticus
    T Iida
    Osaka University, Suita, Osaka, Japan
    Emerg Infect Dis 7:477-8. 2001
    ..We demonstrate that a filamentous phage is specifically associated with the pandemic V. parahaemolyticus strains. An open reading frame unique to the phage is a useful genetic marker to identify these strains...
  5. ncbi request reprint [Type III secretion systems and bacterial pathogenicity]
    Tetsuya Iida
    Tanpakushitsu Kakusan Koso 50:29-35. 2005
  6. pmc Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome
    Tetsuya Iida
    Department of Bacterial Infections Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Bacteriol 184:4933-5. 2002
    ..We demonstrate that filamentous bacteriophages of vibrios, such as f237 (Vibrio parahaemolyticus) and CTXphi (V. cholerae), are integrated into the dif-like site of host chromosome...
  7. pmc Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2
    Toshio Kodama
    Department of Bacterial Infections, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Infect Immun 76:4282-9. 2008
    ..These results indicate that VopB2 and VopD2 act as translocon proteins of V. parahaemolyticus T3SS2 and hence have a critical role in the T3SS2-dependent enterotoxicity of this bacterium...
  8. pmc Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region
    Toshio Kodama
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    PLoS ONE 5:e8678. 2010
    ..These results strongly suggest that VtrA and VtrB are master regulators for virulence gene expression in the Vp-PAI and play critical roles in the pathogenicity of this bacterium...
  9. doi request reprint VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity
    Hirotaka Hiyoshi
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Cell Host Microbe 10:401-9. 2011
    ..cholerae strain. These results indicate that the F-actin-targeting effector VopV is involved in enterotoxic activity of T3SS2-possessing bacterial pathogens...
  10. ncbi request reprint Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors: Tir and EspFu/TccP
    Vlademir V Cantarelli
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Microbiol 9:1782-95. 2007
    ..Binding of cortactin to these effectors is dependent upon tyrosine phosphorylation and a balance between tyrosine phosphorylation and dephosphorylation of cortactin is required to regulate pedestal formation by EHEC...
  11. pmc Functional characterization of two type III secretion systems of Vibrio parahaemolyticus
    Kwon Sam Park
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, 565 0871, Japan
    Infect Immun 72:6659-65. 2004
    ....
  12. pmc Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level
    Natsumi Okada
    Department of Bacterial Infections, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Infect Immun 77:904-13. 2009
    ..Our findings demonstrate that these two distinct types are distributed not only within a species but also beyond the species level and provide a new insight into the pathogenicity and evolution of Vibrio species...
  13. ncbi request reprint Translocation of VP1686 upregulates RhoB and accelerates phagocytic activity of macrophage through actin remodeling
    Rabindra N Bhattacharjee
    Akira Innate Immunity Project, Exploratory Research for Advance Technology, Japan Science and Technology Agency, Osaka University, Osaka 565 0871, Japan
    J Microbiol Biotechnol 18:171-5. 2008
    ..This provides evidence for functional association of the VP1686 in triggering an eat-me-and-die signal to the host...
  14. doi request reprint Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in Escherichia coli K-12 strain as a molecular syringe
    Yukihiro Akeda
    Laboratory of Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Biochem Biophys Res Commun 427:242-7. 2012
    ..coli K-12 strain to inject foreign protein, as well as the V. parahaemolyticus T3SS effector, into cultured cells. These results demonstrate a prospective useful tool with experimental and therapeutic applications...
  15. doi request reprint Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to β4-integrin expressing cell lines
    Said Kamal Abolghait
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, 565 0871 Osaka, Japan
    Microb Pathog 51:454-65. 2011
    ..As far as we know, this is a novel TTSS effector which specifically inactivates Rac1 to disrupt actin cytoskeleton and has an alternative cytotoxic pathway through β4-integrin mediation...
  16. pmc Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity, enterotoxicity, and lethality in mice
    Hirotaka Hiyoshi
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Infect Immun 78:1772-80. 2010
    ..Our findings provide new indications that these virulence factors contribute to and orchestrate each distinct aspect of the pathogenicity of V. parahaemolyticus...
  17. pmc Comparative genomic analysis using microarray demonstrates a strong correlation between the presence of the 80-kilobase pathogenicity island and pathogenicity in Kanagawa phenomenon-positive Vibrio parahaemolyticus strains
    Kaori Izutsu
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Infect Immun 76:1016-23. 2008
    ..These results strongly suggest that acquisition of this Vp-PAI was crucial for the emergence of V. parahaemolyticus strains that are pathogenic for humans...
  18. ncbi request reprint VP1686, a Vibrio type III secretion protein, induces toll-like receptor-independent apoptosis in macrophage through NF-kappaB inhibition
    Rabindra N Bhattacharjee
    Akira Innate Immunity Project, Exploratory Research for Advance Technology, Japan Science and Technology Agency, Osaka 565 0871, Japan
    J Biol Chem 281:36897-904. 2006
    ..Our results suggest an important role for Vibrio effector protein VP1686 that activate a conserved apoptotic pathway in macrophages through suppression of NF-kappaB activation independent of Toll-like receptor signaling...
  19. ncbi request reprint Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2
    Toshio Kodama
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Cell Microbiol 9:2598-609. 2007
    ..We also demonstrate that VopT ADP-ribosylates Ras, a member of the low-molecular-weight G (LMWG) proteins both in vivo and in vitro. These results indicate that VopT is a novel ADPRT effector secreted via V. parahaemolyticus T3SS...
  20. doi request reprint Identification of the Vibrio parahaemolyticus type III secretion system 2-associated chaperone VocC for the T3SS2-specific effector VopC
    Yukihiro Akeda
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    FEMS Microbiol Lett 324:156-64. 2011
    ..These findings, in addition to the previously identified T3SS1-specific chaperone, VecA, provide a strategy to clarify the specificity of effector secretion through T3SSs of V. parahaemolyticus...
  21. pmc Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants
    Kazuyoshi Gotoh
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    PLoS ONE 5:e13365. 2010
    ..The results also reveal a new therapeutic potential for widely used bile acid sequestrants in enteric bacterial infections...
  22. ncbi request reprint [Pathogenicity and genome of Vibrio parahaemolyticus]
    Tetsuya Iida
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita
    Nihon Saikingaku Zasshi 59:457-64. 2004
  23. pmc A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes
    Shigeaki Matsuda
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    PLoS Pathog 8:e1002803. 2012
    ..Therefore, our data suggest that the bacterial effector VepA targets subunit c of V-ATPase and induces the rupture of host cell lysosomes and subsequent cell death...
  24. pmc BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks
    Shinya Yonogi
    Division of Bacteriology, Department of Infectious Disease, Osaka Prefectural Institute of Public Health, Osaka, Osaka, Japan
    Infect Immun 82:2390-9. 2014
    ..Additionally, the presence of becAB on nearly identical plasmids in distinct lineages of C. perfringens isolates suggests the involvement of horizontal gene transfer in the acquisition of the toxin genes. ..
  25. ncbi request reprint The EspB protein of enterohaemorrhagic Escherichia coli interacts directly with alpha-catenin
    Toshio Kodama
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Cell Microbiol 4:213-22. 2002
    ..We conclude that EspB recruits alpha-catenin at the EHEC adherence site by direct interaction, and that the recruitment of alpha-catenin is essential for EHEC-induced A/E lesion formation...
  26. pmc Association of Vibrio parahaemolyticus thermostable direct hemolysin with lipid rafts is essential for cytotoxicity but not hemolytic activity
    Shigeaki Matsuda
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Infect Immun 78:603-10. 2010
    ..We hypothesize that TDH may target a certain group of rafts that are sensitive to SMase at a certain concentration, which does not affect other types of rafts...
  27. ncbi request reprint Escherichia coli verotoxin 1 mediates apoptosis in human HCT116 colon cancer cells by inducing overexpression of the GADD family of genes and S phase arrest
    Rabindra N Bhattacharjee
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    FEBS Lett 579:6604-10. 2005
    ..We therefore suggest that GADD proteins might play an important role in VT1 induced S phase arrest and programmed cell death in HCT116 cells...
  28. doi request reprint Homo-trimeric Structure of the Type IVb Minor Pilin CofB Suggests Mechanism of CFA/III Pilus Assembly in Human Enterotoxigenic Escherichia coli
    Kazuki Kawahara
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 428:1209-26. 2016
    ....
  29. ncbi request reprint [Vibrios (Vibrio cholerae, V. parahaemolyticus, V. vulnificus)]
    Tetsuya Iida
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University
    Nihon Rinsho 61:722-6. 2003
  30. ncbi request reprint Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae
    Kozo Makino
    Department of Molecular Microbiology, Osaka University, Osaka, Japan
    Lancet 361:743-9. 2003
    ..The organism is phylogenetically close to V cholerae, the causative agent of cholera...
  31. pmc Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010
    Mayo Yasugi
    International Research Center for Infectious Diseases, Research Institute for Microbial Diseases RIMD, Osaka University, Suita, Osaka, Japan
    PLoS ONE 7:e30946. 2012
    ..It has been suggested that the virus can bind to both 2,3- and 2,6-linked sialic acid receptors in infected mammals, in contrast to contemporary seasonal H1N1 viruses, which have a predilection for 2,6-linked sialic acid...
  32. pmc Presence of genes for type III secretion system 2 in Vibrio mimicus strains
    Natsumi Okada
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    BMC Microbiol 10:302. 2010
    ..cholerae non-O1/non-O139 serogroup strains. However, whether T3SS2-related genes are present in other Vibrio species remains unclear...
  33. pmc Identification of proteins secreted via Vibrio parahaemolyticus type III secretion system 1
    Takahiro Ono
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Infect Immun 74:1032-42. 2006
    ..Adenylate cyclase fusion protein studies proved that the newly identified secreted proteins were translocated into HeLa cells. Thus, these appear to be the TTSS effector proteins in V. parahaemolyticus...
  34. ncbi request reprint Two type IV pili of Vibrio parahaemolyticus play different roles in biofilm formation
    Akiko Shime-Hattori
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    FEMS Microbiol Lett 264:89-97. 2006
    ..Collectively, these data suggest that the use of two type IV pili is a refined strategy of V. parahaemolyticus for survival in natural environments...
  35. doi request reprint Transcription of Vibrio parahaemolyticus T3SS1 genes is regulated by a dual regulation system consisting of the ExsACDE regulatory cascade and H-NS
    Toshio Kodama
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    FEMS Microbiol Lett 311:10-7. 2010
    ..These findings indicate that the transcription of V. parahaemolyticus T3SS1 genes is regulated by a dual regulatory system consisting of the ExsACDE regulatory cascade and H-NS...
  36. doi request reprint Identification and characterization of a type III secretion-associated chaperone in the type III secretion system 1 of Vibrio parahaemolyticus
    Yukihiro Akeda
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    FEMS Microbiol Lett 296:18-25. 2009
    ..These findings will provide a strategy to clarify how the T3SS1 of V. parahaemolyticus secretes its specific effectors...
  37. doi request reprint Crystal structure of the ADP-ribosylating component of BEC, the binary enterotoxin of Clostridium perfringens
    Kazuki Kawahara
    Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Biochem Biophys Res Commun 480:261-267. 2016
    ..Our results for BECa provide insight into the common catalytic mechanism of the family of binary actin ADPRTs...
  38. pmc Cloning, expression, purification, crystallization and X-ray crystallographic analysis of CofB, the minor pilin subunit of CFA/III from human enterotoxigenic Escherichia coli
    Kazuki Kawahara
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr F Struct Biol Commun 71:663-7. 2015
    ..0 to ∼ 2.0 Å. The initial phase was solved by the single-wavelength anomalous dispersion (SAD) method using a dehydrated SeMet CofB crystal, which resulted in an interpretable electron-density map...
  39. pmc Metagenomic diagnosis of bacterial infections
    Shota Nakamura
    International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Emerg Infect Dis 14:1784-6. 2008
    ..Sequences showing best matches for Campylobacter jejuni were detected only in the illness sample. Various bacteria may be detectable with this metagenomic approach...
  40. doi request reprint Fatal sepsis caused by an unusual Klebsiella species that was misidentified by an automated identification system
    Masafumi Seki
    Division of Infection Control and Prevention, Osaka University, Suita City, Osaka, Japan
    J Med Microbiol 62:801-3. 2013
    ..pneumoniae, which was identified using an automated identification system, but next-generation sequencing and the non-fermentation of adonitol finally identified the cause of sepsis as K. variicola...
  41. pmc Differential replication dynamics for large and small Vibrio chromosomes affect gene dosage, expression and location
    Rikard Dryselius
    Laboratory of Genomic Research on Pathogenic Bacteria, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    BMC Genomics 9:559. 2008
    ..For vibrios, a diverse family of fast growing gammaproteobacteria, gene dosage may be particularly important as they harbor two chromosomes of different size...
  42. ncbi request reprint Demonstration and characterization of manganese superoxide dismutase of Providencia alcalifaciens
    Xiuhao Chen
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Microbiol Immunol 51:951-61. 2007
    ..alcalifaciens. These results suggested that manganese superoxide dismutase plays an important role in intracellular survival of P. alcalifaciens...
  43. ncbi request reprint Cortactin is essential for F-actin assembly in enteropathogenic Escherichia coli (EPEC)- and enterohaemorrhagic E. coli (EHEC)-induced pedestals and the alpha-helical region is involved in the localization of cortactin to bacterial attachment sites
    Vlademir V Cantarelli
    Research Institute for Microbial Diseases, Department of Infectious Diseases, Osaka University, Osaka, Japan
    Cell Microbiol 8:769-80. 2006
    ..These results demonstrate that cortactin is a requirement for pedestal formation and suggest a novel function for the predicted alpha-helical region of cortactin in actin assembly induced by EPEC and EHEC...
  44. pmc Interaction between the type III effector VopO and GEF-H1 activates the RhoA-ROCK pathway
    Hirotaka Hiyoshi
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    PLoS Pathog 11:e1004694. 2015
    ..Thus, VopO hijacks the RhoA-ROCK pathway in a different manner compared with previously reported bacterial toxins and effectors that modulate the Rho GTPase signaling pathway. ..
  45. pmc Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach
    Shota Nakamura
    Department of Genome Informatics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    PLoS ONE 4:e4219. 2009
    ....
  46. pmc The Vibrio parahaemolyticus effector VopC mediates Cdc42-dependent invasion of cultured cells but is not required for pathogenicity in an animal model of infection
    Ryu Okada
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Cell Microbiol 16:938-47. 2014
    ..Thus, although VopC can promote host cell invasion, such internalization is not a critical step of the disease process, consistent with the traditional view of V. parahaemolyticus as an extracellular pathogen. ..
  47. pmc Comparison of genome structures of vibrios, bacteria possessing two chromosomes
    Kenichi Tagomori
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Bacteriol 184:4351-8. 2002
    ..The difference in the orders of the ORFs on the chromosomes of N16961 and KX-V237 implies that numerous and frequent genetic exchanges have occurred intrachromosomally rather than interchromosomally...
  48. pmc vpaH, a gene encoding a novel histone-like nucleoid structure-like protein that was possibly horizontally acquired, regulates the biogenesis of lateral flagella in trh-positive Vibrio parahaemolyticus TH3996
    Kwon Sam Park
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
    Infect Immun 73:5754-61. 2005
    ..In conclusion, our findings suggest that VpaH affects lateral flagellum biogenesis in trh-positive V. parahaemolyticus strain TH3996...
  49. ncbi request reprint Microarray analysis identifies apoptosis regulatory gene expression in HCT116 cells infected with thermostable direct hemolysin-deletion mutant of Vibrio parahaemolyticus
    Rabindra N Bhattacharjee
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biochem Biophys Res Commun 335:328-34. 2005
    ..Our results strongly suggest that apoptosis requires functional TTSS1 and TTSS1-dependent translocation factor(s) to be associated with the host cell death, and thus pathogenesis of V. parahaemolyticus...
  50. pmc A repeat unit of Vibrio diarrheal T3S effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments
    Mitsuhiro Nishimura
    1 Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan 2 Osaka University of Pharmaceutical Sciences, 4 20 1 Nasahara, Takatsuki, Osaka, 569 1094, Japan
    Sci Rep 5:10870. 2015
    ..The phalloidin-like behavior, targeting the interstrand region of actin filaments to stabilize the filament structure, likely contributes to the pathogenicity of V. parahaemolyticus...
  51. pmc Performance comparison of second- and third-generation sequencers using a bacterial genome with two chromosomes
    Mari Miyamoto
    Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    BMC Genomics 15:699. 2014
    ....
  52. doi request reprint Regulation of Vibrio parahaemolyticus T3SS2 gene expression and function of T3SS2 effectors that modulate actin cytoskeleton
    Toshio Kodama
    Pathogenic Microbes Repository Unit, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Cell Microbiol 17:183-90. 2015
    ..This review focuses on the regulation of T3SS2 gene expression and T3SS2 effectors that specifically target the actin cytoskeleton. ..
  53. pmc Precise region and the character of the pathogenicity island in clinical Vibrio parahaemolyticus strains
    Tomohiko Sugiyama
    Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Bacteriol 190:1835-7. 2008
    ..Vp-PAI has features in common with Tn7 and other related elements at both terminal ends. Our findings indicate that the mobile element with a transposase which contains the DDE motif may have been involved in Vp-PAI formation...
  54. pmc Vibrios commonly possess two chromosomes
    Kazuhisa Okada
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Bacteriol 187:752-7. 2005
    ..8 to 2.4 Mb). This fact suggests that the structure of the small chromosome is more flexible than that of the large chromosome during the evolution of vibrios...
  55. ncbi request reprint Urease activity of enterohaemorrhagic Escherichia coli depends on a specific one-base substitution in ureD
    Masayuki Nakano
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita, Osaka, 565 0871, Japan
    Microbiology 150:3483-9. 2004
    ..These results suggest that the lack of urease activity in most EHEC strains is due to a premature stop codon in ureD...
  56. ncbi request reprint Cytotoxicity and enterotoxicity of the thermostable direct hemolysin-deletion mutants of Vibrio parahaemolyticus
    Kwon Sam Park
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Japan
    Microbiol Immunol 48:313-8. 2004
    ..These results indicate that the cytotoxicity and enterotoxicity of TDH-producing V. parahaemolyticus are not explained by TDH alone, and suggest that an unknown virulence factor(s) could be involved in these pathogenic activities...
  57. pmc Genetic characterization of DNA region containing the trh and ure genes of Vibrio parahaemolyticus
    K S Park
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Infect Immun 68:5742-8. 2000
    ....
  58. pmc Cortactin is necessary for F-actin accumulation in pedestal structures induced by enteropathogenic Escherichia coli infection
    Vlademir V Cantarelli
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Infect Immun 70:2206-9. 2002
    ..Cortactin is shown to be necessary for organizing actin pedestals in response to EPEC, based on the expression of green fluorescent protein-fused cortactin derivatives in HeLa cells...
  59. ncbi request reprint Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 Å resolution by sulfur-SAD phasing
    Shunsuke Fukakusa
    Department of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr D Biol Crystallogr 68:1418-29. 2012
    ..These characteristics could provide a unique binding epitope for the CFA/III pili of ETEC compared with other type IVb pili...
  60. doi request reprint Metagenomic analysis of bacterial infections by means of high-throughput DNA sequencing
    Shota Nakamura
    Department of Infection Metagenomics, Genome Information Research Center, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Exp Biol Med (Maywood) 236:968-71. 2011
    ..Also, the approach enables us to conduct an unbiased analysis of dynamics of the pathogen and associated microbiota in human specimens during the course of infectious diseases...
  61. ncbi request reprint Vibrionaceae, a versatile bacterial family with evolutionarily conserved variability
    Rikard Dryselius
    Laboratory of Genomic Research on Pathogenic Bacteria, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Res Microbiol 158:479-86. 2007
    ..Based on complete genome sequences and the findings generated therefrom, we discuss the origin, evolution and stability of this unusual chromosomal arrangement as well as its possible benefits...
  62. ncbi request reprint Quantitative detection of periodontal pathogens using real-time polymerase chain reaction with TaqMan probes
    M Kuboniwa
    Department of Preventive Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan
    Oral Microbiol Immunol 19:168-76. 2004
    ..These results suggest that the present real-time PCR method with the designed probe/primer sets enabled sensitive detection of the six periodontal bacteria, and may also assist future microbial studies of periodontal diseases...
  63. doi request reprint Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia
    Ryu Okumura
    Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 532:117-21. 2016
    ..Lypd8 bound to flagella and suppressed motility of flagellated bacteria. Thus, Lypd8 mediates segregation of intestinal bacteria and epithelial cells in the colon to preserve intestinal homeostasis. ..
  64. pmc Metagenomic Analysis Reveals Dynamic Changes of Whole Gut Microbiota in the Acute Phase of Intensive Care Unit Patients
    Masahiro Ojima
    Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, 565 0871, Japan
    Dig Dis Sci 61:1628-34. 2016
    ..Metagenomic analysis targeting the 16S rRNA gene has made it possible to characterize the vast array of microorganisms contained in the gut...
  65. doi request reprint Dysbiosis Contributes to Arthritis Development via Activation of Autoreactive T Cells in the Intestine
    Yuichi Maeda
    Osaka University, Osaka, Japan, and Japan Agency for Medical Research and Development, Tokyo, Japan
    Arthritis Rheumatol 68:2646-2661. 2016
    ..The aim of this study was to investigate whether altered composition of human intestinal microbiota in RA patients contributes to the development of arthritis...
  66. doi request reprint Complete nucleotide sequence of a plasmid containing the botulinum neurotoxin gene in Clostridium botulinum type B strain 111 isolated from an infant patient in Japan
    Koji Hosomi
    Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1 58 Rinku Orai Kita, Izumisano, Osaka, 598 8531, Japan
    Mol Genet Genomics 289:1267-74. 2014
    ..botulinum strains. It was suggested that these plasmids had been derived from a common ancestor and had played important roles for the bont gene transfer between C. botulinum...
  67. ncbi request reprint Chloride secretion induced by thermostable direct haemolysin of Vibrio parahaemolyticus depends on colonic cell maturation
    A Takahashi
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    J Med Microbiol 50:870-8. 2001
    ..These results suggest that sensitivity to TDH is affected by the stage of cellular differentiation of cultured intestinal epithelial cells...
  68. ncbi request reprint A large outbreak of foodborne infection attributed to Providencia alcalifaciens
    T Murata
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    J Infect Dis 184:1050-5. 2001
    ..alcalifaciens. This is the first report of a large outbreak of foodborne infection attributed to the organism and provides definitive evidence that P. alcalifaciens is a causative agent of gastroenteritis...
  69. pmc A filamentous phage associated with recent pandemic Vibrio parahaemolyticus O3:K6 strains
    H Nasu
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Clin Microbiol 38:2156-61. 2000
    ..parahaemolyticus O3:K6 strains. The ORF8 gene can be a useful genetic marker for the identification of the recently widespread O3:K6 strains of V. parahaemolyticus...
  70. pmc Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by a clinically isolated Vibrio parahaemolyticus strain, TH3766
    M Xu
    Research Institute for Microbial Diseases, Osaka University, Japan
    Infect Immun 62:166-71. 1994
    ..Thus, these findings suggest that the TH3766 strain produces two types of hemolysins simultaneously. This is the first evidence that a strain of V. parahaemolyticus produces two types of toxins of the TDH-TRH family at the same time...
  71. pmc Dominant-negative Rho, Rac, and Cdc42 facilitate the invasion process of Vibrio parahaemolyticus into Caco-2 cells
    Yukihiro Akeda
    Department of Bacterial Infections Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Infect Immun 70:970-3. 2002
    ..This assay showed that the dominant negative host phenotype facilitates bacterial invasion, suggesting that the mechanism of V. parahaemolyticus invasion differs from that reported for other invasive bacteria...
  72. doi request reprint Generation of colonic IgA-secreting cells in the caecal patch
    Kazunori Masahata
    1 Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565 0871, Japan 2 Department of Pediatric Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Nat Commun 5:3704. 2014
    ..Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine. ..
  73. ncbi request reprint Monodansylcadaverine inhibits cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin on cultured rat embryonic fibroblast cells
    R Naim
    Department of Bacterial Infection, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEMS Microbiol Lett 196:99-105. 2001
    ..On the hemolytic activity of TDH, monodansylcadaverine and EGTA had no effect. These results suggest that the mechanism of cytotoxicity of TDH on Rat-1 cells was different from that of hemolytic activity of TDH on red blood cells...
  74. pmc Association of the urease gene with enterohemorrhagic Escherichia coli strains irrespective of their serogroups
    M Nakano
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    J Clin Microbiol 39:4541-3. 2001
    ..The results suggest that the urease gene can be a useful genetic marker for the detection of EHEC strains and for the diagnosis of infections caused by EHEC strains in the clinical situation...
  75. ncbi request reprint Mechanisms of chloride secretion induced by thermostable direct haemolysin of Vibrio parahaemolyticus in human colonic tissue and a human intestinal epithelial cell line
    A Takahashi
    Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Japan
    J Med Microbiol 49:801-10. 2000
    ..Thus, TDH increases Cl- secretion in human colonic epithelial cells, apparently through mechanisms involving cell binding and Ca2+ influx, followed by elevation of [Ca2+]in associated with PKC phosphorylation...
  76. ncbi request reprint Cloning and expression of two genes encoding highly homologous hemolysins from a Kanagawa phenomenon-positive Vibrio parahaemolyticus T4750 strain
    T Iida
    Department of Bacteriology and Serology, Osaka University, Japan
    Gene 93:9-15. 1990
    ..These data suggest that tdhA is the structural gene for TDH found in the culture supernatant of V. parahaemolyticus T4750, and that there was only partial, if any, tdhS expression in the strain T4750 under the test conditions employed...
  77. ncbi request reprint Thermostable direct hemolysin of Vibrio parahaemolyticus is a bacterial reversible amyloid toxin
    Takashi Fukui
    Department of Developmental Infectious Diseases, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo cho, Izumi, Osaka 594 1101, Japan
    Biochemistry 44:9825-32. 2005
    ..Congo red was found to inhibit the hemolytic activity of TDH in a dose-dependent manner. These data reveal that the mechanism of the Arrhenius effect which is tightly related to the fibrillogenicity of TDH...
  78. ncbi request reprint The urease gene cluster of Vibrio parahaemolyticus does not influence the expression of the thermostable direct hemolysin (TDH) gene or the TDH-related hemolysin gene
    Yoshitsugu Nakaguchi
    Graduate School of Medicine, Kyoto University, Kyoto, Kyoto 606 8501, Japan
    Microbiol Immunol 47:233-9. 2003
    ..parahaemolyticus did not change by introduction of the urease gene cluster either. It was concluded therefore that the urease gene cluster is not involved in the regulation of tdh and trh expression...
  79. pmc Biodiversity of vibrios
    Fabiano L Thompson
    Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, Ghent 9000, Belgium
    Microbiol Mol Biol Rev 68:403-31, table of contents. 2004
    ..g., microarrays will facilitate the investigation of the gene repertoire at the species level. Based on such new genomic information, the taxonomy and the species concept for vibrios will be reviewed in the next years...
  80. ncbi request reprint Single channel evidence for innate pore-formation by Vibrio parahaemolyticus thermostable direct haemolysin (TDH) in phospholipid bilayers
    Simon P Hardy
    School of Pharmacy and Biomolecular Sciences, University of Brighton, Lewes Road, Brighton, Sussex BN2 4GJ, UK
    FEMS Microbiol Lett 240:81-5. 2004
    ..Whilst various techniques have previously suggested that TDH is a pore-forming toxin, the data in this study provide direct single channel evidence and indicate several features of pore formation in synthetic phospholipid membranes...
  81. ncbi request reprint Lateral transfer of the lux gene cluster
    Sabu Kasai
    Department of Applied Chemistry and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3 3 138, Sumiyoshi ku, Osaka 558 8585, Japan
    J Biochem 141:231-7. 2007
    ..The undigested genomic DNAs of five luminous species were analysed by pulsed-field gel electrophoresis and Southern hybridization. In all the species except V. cholerae, the operons are located on chromosome 2...