Hiroshi Suzuki

Summary

Affiliation: Obihiro University of Agriculture and Veterinary Medicine
Country: Japan

Publications

  1. doi request reprint Cryopreservation of canine embryos and resulting pregnancies
    H Suzuki
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
    Reprod Domest Anim 47:141-3. 2012
  2. pmc Effect of 50 Hz electric field in diacylglycerol acyltransferase mRNA expression level and plasma concentration of triacylglycerol, free fatty acid, phospholipid and total cholesterol
    Takuya Hori
    Hakuju Institute for Health Science, Tokyo 151 0063, Japan
    Lipids Health Dis 11:68. 2012
  3. pmc Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion
    Kenta Watanabe
    Department of Veterinary Public Health, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan
    BMC Microbiol 8:212. 2008
  4. pmc Loss of Parp-1 affects gene expression profile in a genome-wide manner in ES cells and liver cells
    Hideki Ogino
    ADP ribosylation in Oncology Project, National Cancer Center Research Institute, 1 1, Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    BMC Genomics 8:41. 2007
  5. pmc Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation
    Hiroshi Suzuki
    National Research Center of Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 080 8555, Japan
    J Assist Reprod Genet 25:571-5. 2008
  6. ncbi request reprint Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli
    Irungu Gaturaga
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 91:191-2. 2005
  7. ncbi request reprint Serodiagnosis of canine Babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant P50 expressed in Escherichia coli
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 90:387-91. 2004
  8. doi request reprint Cloning, characterization and validation of inosine 5'-monophosphate dehydrogenase of Babesia gibsoni as molecular drug target
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Parasitol Int 62:87-94. 2013
  9. pmc Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Infect Immun 72:1795-8. 2004
  10. doi request reprint Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Acta Parasitol 58:619-23. 2013

Collaborators

Detail Information

Publications85

  1. doi request reprint Cryopreservation of canine embryos and resulting pregnancies
    H Suzuki
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
    Reprod Domest Anim 47:141-3. 2012
    ..Such technology would also facilitate the transportation and storage of genetic materials and aid in the elimination of vertically transmitting diseases in the dog...
  2. pmc Effect of 50 Hz electric field in diacylglycerol acyltransferase mRNA expression level and plasma concentration of triacylglycerol, free fatty acid, phospholipid and total cholesterol
    Takuya Hori
    Hakuju Institute for Health Science, Tokyo 151 0063, Japan
    Lipids Health Dis 11:68. 2012
    ....
  3. pmc Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion
    Kenta Watanabe
    Department of Veterinary Public Health, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan
    BMC Microbiol 8:212. 2008
    ..In the present study, we investigated mechanism of B. abortus invasion into TG cells...
  4. pmc Loss of Parp-1 affects gene expression profile in a genome-wide manner in ES cells and liver cells
    Hideki Ogino
    ADP ribosylation in Oncology Project, National Cancer Center Research Institute, 1 1, Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
    BMC Genomics 8:41. 2007
    ..However, the impact of Parp-1-deficiency on the regulation of genome-wide gene expression has not been fully studied yet...
  5. pmc Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation
    Hiroshi Suzuki
    National Research Center of Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 080 8555, Japan
    J Assist Reprod Genet 25:571-5. 2008
    ..Thus, effects of erythropoietin (EPO) and desialylated EPO administration on the frozen-thawed canine ovarian xenotransplantation were examined...
  6. ncbi request reprint Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli
    Irungu Gaturaga
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 91:191-2. 2005
    ..6%) were confirmed as positive by Western blot analysis with whole-parasite antigens. These results suggest that ELISA with recombinant NcSRS2t is an effective method for diagnosis of N. caninum infection in cattle...
  7. ncbi request reprint Serodiagnosis of canine Babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant P50 expressed in Escherichia coli
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 90:387-91. 2004
    ..These results indicate that the recombinant P50 expressed in E. coil is a useful diagnostic antigen for practical use in the diagnosis of B. gibsoni infection in dogs...
  8. doi request reprint Cloning, characterization and validation of inosine 5'-monophosphate dehydrogenase of Babesia gibsoni as molecular drug target
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Parasitol Int 62:87-94. 2013
    ..95±0.21 and 2.88±0.49μM for B. gibsoni and B. bovis, respectively. Therefore, our results suggest that MPA may inhibit the replication of Babesia parasites by targeting IMPDH enzyme of the purine pathway...
  9. pmc Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Infect Immun 72:1795-8. 2004
    ..The antiserum to the recombinant P50 protein significantly inhibited the parasite growth, indicating that P50 might be a useful vaccine candidate...
  10. doi request reprint Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Acta Parasitol 58:619-23. 2013
    ..These results indicated that this type of DNA/rvv prime-boost immunization approach may have use against B. gibsoni infection in dogs. ..
  11. pmc Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555 Japan
    Malar J 9:101. 2010
    ..However, mechanisms of this Plasmodium inhibition, in addition to means by which to exploit this finding as a therapeutic strategy, remain unclear...
  12. doi request reprint Immunogenicity and growth inhibitory efficacy of the prime-boost immunization regime with DNA followed by recombinant vaccinia virus carrying the P29 gene of Babesia gibsoni in dogs
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 123:296-301. 2009
    ..Moreover, the P29 immunized group showed a significantly low level of parasitemia. In conclusion, this study supports the efficacy of a prime-boost strategy for dogs against canine B. gibsoni infection...
  13. pmc High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 10:596-601. 2003
    ..canis-infected dog serum or noninfected dog serum. Our results indicated that the rP50t may provide a useful potential immunogenic reagent for use in diagnosis and as a subunit vaccine to control B. gibsoni infection in dogs...
  14. ncbi request reprint Isolation and identification of an actin gene from Babesia gibsoni
    Jinlin Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 92:208-10. 2006
    ..Immunofluorescence and confocal microscopic observation revealed that the protein is diffusely distributed throughout the B. gibsoni parasites...
  15. ncbi request reprint Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects
    Hiroaki Shirafuji
    National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokaido, Japan
    J Parasitol 91:476-9. 2005
    ..parvum. These findings indicate that the T. gondii vector may provide a new tool for the production of a recombinant vaccine against cryptosporidiosis in animals...
  16. doi request reprint Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni
    Youn Kyoung Goo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 190:595-8. 2012
    ..The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection...
  17. doi request reprint Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Parasitol Res 111:1259-66. 2012
    ..Overall, we have demonstrated a high prevalence and polymorphism of Babesia parasites in northern Thailand calling for the need to design effective control programs for bovine babesiosis...
  18. doi request reprint The effect of alpha-tocopherol transfer protein gene disruption on Trypanosoma congolense infection in mice
    Maria Shirley Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro 080 8555, Japan
    Free Radic Biol Med 47:1408-13. 2009
    ..These results suggest that transient inhibition of alpha-tocopherol transfer gene activity could possibly be exploited as a strategy for both the prevention and the treatment of trypanosomiasis...
  19. ncbi request reprint Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense
    Tomoko Suzuki
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 74:1020-5. 2006
    ..These results, taken together, indicate that erythropoietin administration is effective for the treatment of T. congolense infection...
  20. doi request reprint Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA)
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 133:346-52. 2013
    ..microti challenge, but no significant protection was observed. Our data suggest that the immunodominant antigen BmIRA could be a useful serodiagnostic antigen for screening of B. microti infection...
  21. pmc Immunization with recombinant surface antigen P50 of Babesia gibsoni expressed in insect cells induced parasite growth inhibition in dogs
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Clin Diagn Lab Immunol 12:557-9. 2005
    ..Dogs immunized with P50 showed partial protection manifested as a significantly low level of parasitemia. The results indicated that P50 is a primary vaccine candidate molecule against canine B. gibsoni infection...
  22. pmc High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis
    Xiaohong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Clin Microbiol 41:1147-51. 2003
    ..The results indicate that G-rEMA-2t and rEMA-2t proteins should be suitable antigens for the development of an effective immunodiagnostic assay due to their high sensitivity, specificity, and great yield...
  23. pmc Cloning, expression, and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: inhibitory effect of antifolates on its catalytic activity and parasite proliferation
    Gabriel O Aboge
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Antimicrob Agents Chemother 52:4072-80. 2008
    ..gibsoni DHFR could be a model for studying antifolate compounds as potential drug candidates. Therefore, the B. gibsoni DHFR-TS is a molecular antifolate drug target...
  24. ncbi request reprint A novel 57-kDa merozoite protein of Babesia gibsoni is a prospective antigen for diagnosis and serosurvey of canine babesiosis by enzyme-linked immunosorbent assay
    Gabriel Oluga Aboge
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 149:85-94. 2007
    ..gibsoni antibodies. Overall, the rBgP57-ELISA and seminested PCR when used in combination, could improve epidemiological surveys and clinical diagnosis of B. gibsoni infection...
  25. ncbi request reprint Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase
    Ganbat Tsogtbaatar
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 70:1387-9. 2008
    ..The recombinant SOD showed a specific reaction with serum infected with B. canis in Western blotting and ELISA. These results suggest that ELISA using recombinant SOD is useful in screening for canine brucellosis...
  26. pmc Pronuclear formation of freeze-dried canine spermatozoa microinjected into mouse oocytes
    Hiroyuki Watanabe
    Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 8555, Japan
    J Assist Reprod Genet 26:531-6. 2009
    ..The aim of the present study was to investigate the fertilizing capacity of fresh, frozen-thawed and freeze-dried canine spermatozoa...
  27. ncbi request reprint Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis
    Honglin Jia
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 75:843-50. 2006
    ..gibsoni. These results indicated that BgSA1 could be a useful target for the development of a diagnostic test for the detection of specific antibodies and circulating antigens...
  28. pmc Roles of the Maltese cross form in the development of parasitemia and protection against Babesia microti infection in mice
    Naoaki Yokoyama
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Infect Immun 71:411-7. 2003
    ..microti infection. These findings indicate that the Maltese cross form plays important roles in both the development of parasitemia and the protective response against the infection...
  29. doi request reprint Primary Babesia rodhaini infection followed by recovery confers protective immunity against B. rodhaini reinfection and Babesia microti challenge infection in mice
    Guanbo Wang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Exp Parasitol 169:6-12. 2016
    ..The above results proved that mice immunized with alive B. rodhaini could acquire protective immunity against B. rodhaini and B. microti infections. ..
  30. pmc Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey
    Mo Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
    J Vet Med Sci . 2016
    ..8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T. gondii and N. caninum infections are prevalent in Turkish domestic animals...
  31. pmc Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
    Mo Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080 8555, Japan
    Parasit Vectors 9:257. 2016
    ..canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection...
  32. doi request reprint Alpha-tocopherol transfer protein gene inhibition enhances the acquired immune response during malaria infection in mice
    Maria Shirley Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555, Japan
    Parasitol Res 113:1019-27. 2014
    ..The results suggest that inhibition of α-tocopherol transfer protein activity is effective for the enhancement of acquired immunity in murine malaria infection...
  33. doi request reprint Mycophenolic acid, mycophenolate mofetil, mizoribine, ribavirin, and 7-nitroindole inhibit propagation of Babesia parasites by targeting inosine 5'-monophosphate dehydrogenase
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 100:522-6. 2014
    ..microti . Altogether, we provide a basis for development of antibabesia prodrugs by targeting IMPDH of the parasites in the treatment of babesiosis...
  34. ncbi request reprint Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1
    Bayin Chahan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 118:177-85. 2003
    ..These results suggest that the recombinant NcSAG1t could be a reliable reagent for use as an antigen in ELISA for the serodiagnosis of N. caninum infection in cattle...
  35. ncbi request reprint Short report: role of type I/II scavenger receptors in malarial infection in C57BL/6J mice
    Mai Inoue
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 75:178-81. 2006
    ....
  36. doi request reprint Expression of erythropoietic cytokines in α-tocopherol transfer protein knockout mice with murine malaria infection
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555, Japan
    Parasitol Res 109:1243-50. 2011
    ....
  37. ncbi request reprint Regulated upon activation normal T-cell expressed and secreted (RANTES) contributes to abortion caused by Brucella abortus infection in pregnant mice
    Kenta Watanabe
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    J Vet Med Sci 70:681-6. 2008
    ..Neutralization of RANTES, induced by B. abortus infection, served to prevent abortion. These results indicate that the production and function of RANTES are correlated with IFN-gamma in pregnant mice infected with B. abortus...
  38. ncbi request reprint Development of TaqMan-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina
    Chulmin Kim
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 77:837-41. 2007
    ..Further studies will be necessary to investigate the clinical value of this technique, especially for the quantitative detections of the parasites...
  39. ncbi request reprint Expression of amphiregulin during the pre- and post-implantation period in the mouse reproductive tract
    Dong Soo Lee
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Reprod Dev 52:781-7. 2006
    ..However, our present results indicate that amphiregulin mRNA is upregulated by the presence of fertilized eggs in the lumen of the oviduct on 0.5 dpc...
  40. doi request reprint Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand
    Charoonluk Jirapattharasate
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080 8555, Japan
    Parasitol Res . 2016
    ..This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand...
  41. doi request reprint Molecular detection and genetic characterization of Babesia, Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
    Mo Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan Harbin Veterinary Research Institute, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Haping Street 678, Xiangfang District, Harbin 150069, PR China
    Ticks Tick Borne Dis . 2016
    ..To our knowledge, this is the first study on the co-infection of Babesia, Theileria and Anaplasma in sheep and goats in Turkey...
  42. doi request reprint Molecular detection of spotted fever group rickettsiae in Amblyomma variegatum ticks from Benin
    Paul Franck Adjou Moumouni
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501 1193, Japan
    Ticks Tick Borne Dis 7:828-33. 2016
    ..africae and spotted fever rickettsioses in Africa. Clinicians in Benin and those treating travellers should be aware of the possibility of SFG rickettsiae infection when they are treating patients with febrile illness. ..
  43. doi request reprint Genetic variations of four immunodominant antigens of Babesia gibsoni isolated from dogs in southwest Japan
    Mingming Liu
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Ticks Tick Borne Dis 7:298-305. 2016
    ..Our findings provide the genetic basis for designing and testing the efficacy of diagnostic antigens as well as vaccine candidates against B. gibsoni...
  44. doi request reprint Molecular Characterization of Babesia bovis M17 Leucine Aminopeptidase and Inhibition of Babesia Growth by Bestatin
    Gabriel Oluga Aboge
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 101:536-41. 2015
    ..Therefore, BbM17LAP may be a target of bestatin, though more studies with other aminopeptidase inhibitors are required to confirm this...
  45. ncbi request reprint Artificial insemination with canine spermatozoa frozen in a skim milk/glucose-based extender
    Yasuyuki Abe
    Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Reprod Dev 54:290-4. 2008
    ....
  46. ncbi request reprint Effects of exposure to a 50 Hz electric field on plasma levels of lactate, glucose, free Fatty acids, triglycerides and creatine phosphokinase activity in hind-limb ischemic rats
    Shinji Harakawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 67:969-74. 2005
    ..However, further studies are needed to elucidate the association of ELF-EF with the lipid metabolism system...
  47. ncbi request reprint Expression patterns of the implantation-associated genes in the uterus during the estrous cycle in mice
    Dong Soo Lee
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Reprod Dev 51:787-98. 2005
    ..These results indicate that rat USAG-1 mouse homologue expression at implantation might be specifically regulated by embryonic factors rather than the maternal environment...
  48. ncbi request reprint Brucella melitensis infection associated with Guillain-Barré syndrome through molecular mimicry of host structures
    Kenta Watanabe
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    FEMS Immunol Med Microbiol 45:121-7. 2005
    ..melitensis immunized mice. These results suggest that B. melitensis is a new etiological agent for GBS and that immunological responses between it and GBS-associated C. jejuni in the mouse model may be different...
  49. ncbi request reprint Vitamin C deficiency fails to protect mice from malaria
    Maria Shirley Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
    Exp Anim 59:239-43. 2010
    ..The results indicate that deficiency of vitamin C might not affect the development of the malaria parasite in mice...
  50. ncbi request reprint Roles of Brucella abortus SpoT in morphological differentiation and intramacrophagic replication
    Suk Kim
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Microbiology 151:1607-17. 2005
    ..The data suggest that SpoT does not contribute to intracellular trafficking of B. abortus, but contributes to the maintenance of bacterial morphology and the physiological adaptation required for intracellular replication...
  51. ncbi request reprint Cryopreservation of canine ovaries by vitrification
    Takako Ishijima
    Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Reprod Dev 52:293-9. 2006
    ..These results indicate that cryopreservation of the canine ovary by vitrification appears to have the potential to restore endocrine function and ovulation potential...
  52. pmc A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes
    Hiroyuki Watanabe
    Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Japan
    J Assist Reprod Genet 27:581-8. 2010
    ..The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa...
  53. doi request reprint Cryopreservation of canine embryos
    Yasuyuki Abe
    Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
    Biol Reprod 84:363-8. 2011
    ..In addition, this technique will contribute to the improved breeding of companion and working dogs such as guide dogs, drug-detecting dogs, and quarantine dogs...
  54. ncbi request reprint Glycophorin A-knockout mice, which lost sialoglycoproteins from the red blood cell membrane, are resistant to lethal infection of Babesia rodhaini
    Noriyuki Takabatake
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 148:93-101. 2007
    ..These results indicate that RBC sialoglycoproteins lost in GPA(-/-) mice are involved in the in vivo growth of B. rodhaini, probably functioning as essential molecule(s) for the parasite invasion of host RBCs in the blood circulation...
  55. doi request reprint In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers
    Mohammed Ali Abdel-Ghani
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
    Theriogenology 77:347-55. 2012
    ..05), and least oocyte degeneration (P < 0.05) among experimental groups. In conclusion, BGML had a positive impact on the in vitro maturation system, as well as meiotic resumption of canine oocytes...
  56. ncbi request reprint A rapid agglutination assay for canine brucellosis using antigen coated beads
    Masahisa Watarai
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    J Vet Med Sci 69:477-80. 2007
    ....
  57. ncbi request reprint Preimplantation development of embryos in labrador retrievers
    Yasuyuki Abe
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
    J Reprod Dev 54:135-7. 2008
    ....
  58. doi request reprint Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy
    Guanbo Wang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 172:5-11. 2016
    ..These results suggest that combined immunization with rBmAMA1 and rBmRON2 is a promising strategy against B. microti...
  59. doi request reprint Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey
    Mo Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Ticks Tick Borne Dis 7:126-34. 2016
    ..This study provides important data for understanding the epidemiology of tick-borne diseases and it is expected to improve approach for diagnosis and control of tick-borne diseases in Turkey. ..
  60. ncbi request reprint Molecular and seroepidemiological survey of Babesia bovis and Babesia bigemina infections in cattle and water buffaloes in the central region of Vietnam
    Yan Li
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Trop Biomed 31:406-13. 2014
    ..bovis and B. bigemina infections in the central region of Vietnam, which is needed to develop comprehensive approach to the modern surveillance, diagnosis and control of bovine babesiosis. ..
  61. ncbi request reprint Expression of uterine sensitization-associated gene-1 (USAG-1) in the mouse uterus during the peri-implantation period
    Kanami Maeda
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    J Reprod Dev 53:931-6. 2007
    ..Expression of USAG-1 mRNA appears to be associated with blastocyst implantation to the luminal epithelium, suggesting that physiological or biochemical contact of the blastocyst to the uterus is required for USAG-1 expression...
  62. doi request reprint Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice
    Guohong Zhang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vaccine 28:7243-7. 2010
    ..caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine...
  63. doi request reprint Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice
    Masato Tachibana
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro shi, Hokkaido 080 8555, Japan
    Microb Pathog 45:105-9. 2008
    ..abortus infection. These results suggest that down-regulation of HO-1 in TG cells due to B. abortus infection is an important event in infectious abortion...
  64. doi request reprint Molecular survey of canine vector-borne diseases in stray dogs in Thailand
    Mingming Liu
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Int 65:357-61. 2016
    ..The current findings are important for future surveillance of CVBDs and designing appropriate approaches for diagnosis and control for the diseases in Thailand. ..
  65. pmc Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya
    Paul Franck Adjou Moumouni
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080 8555, Japan
    Parasit Vectors 8:496. 2015
    ..This study established the genetic diversities of the above tick-borne hemoparasites infecting cattle in Kenya...
  66. doi request reprint Molecular epidemiology of bovine Babesia spp. and Theileria orientalis parasites in beef cattle from northern and northeastern Thailand
    Charoonluk Jirapattharasate
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Int 65:62-9. 2016
    ..orientalis and elucidate the effect of co-infection with Babesia spp. on the pathogenicity of T. orientalis infection on beef in northern and northeastern Thailand. ..
  67. pmc Macrophages are the determinant of resistance to and outcome of nonlethal Babesia microti infection in mice
    Mohamad Alaa Terkawi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Infect Immun 83:8-16. 2015
    ..microti and imply that the severity of the infection in immunocompromised patients might be due to impairment of macrophage function. ..
  68. doi request reprint alpha-Tocopherol transfer protein inhibition is effective in the prevention of cerebral malaria in mice
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture, Tokyo, Japan
    Am J Clin Nutr 91:200-7. 2010
    ..Despite the evidence of Plasmodium sensitivity to oxidative stress, the potential role of vitamin E, a free radical scavenger, on the outcome of cerebral malaria (CM) has yet to be elucidated...
  69. ncbi request reprint Effects of a 50 Hz electric field on plasma lipid peroxide level and antioxidant activity in rats
    Shinji Harakawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
    Bioelectromagnetics 26:589-94. 2005
    ..Although the administration of AAPH decreased AOA, this decrease did not change when EF was added. These data indicate that the ELF EF used in this study influenced the lipid peroxide level in an oxidatively stressed rat...
  70. pmc Interferon-gamma promotes abortion due to Brucella infection in pregnant mice
    Suk Kim
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    BMC Microbiol 5:22. 2005
    ..In the present study, we investigated abortion induced by Brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, in a mouse model...
  71. pmc Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay
    Xiaohong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 9:1343-7. 2002
    ..The results suggest that S-rSAG2 would be a useful reagent for the detection of T. gondii infection in cats...
  72. ncbi request reprint Detection of Brucella canis and Leptospira interrogans in canine semen by multiplex nested PCR
    Suk Kim
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 68:615-8. 2006
    ..Since they have been found to stay alive in canine semen and CPA up to 48 hr, canine semen for breeding purposes should be checked for contamination by the PCR assay...
  73. doi request reprint Effect of anti-hyperlipidemia drugs on the alpha-tocopherol concentration and their potential for murine malaria infection
    Aiko Kume
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Parasitol Res 115:69-75. 2016
    ..Our finding indicates that plasma alpha-tocopherol reducing drugs like probucol might be a candidate for beneficial prevention strategy for travelers from malaria-free area...
  74. pmc Sexing of dog sperm by fluorescence in situ hybridization
    Maya Oi
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080 8555, Japan
    J Reprod Dev 59:92-6. 2013
    ..6% for the Y chromosome fraction. This sorting was evaluated by using the dual color FISH protocol. Therefore, our results demonstrated that the FISH protocol worked reliably for both unsorted and sexed sperm samples...
  75. doi request reprint Expression analysis of autophagy-related genes in the hard tick Haemaphysalis longicornis
    Rika Umemiya-Shirafuji
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro 080 8555, Hokkaido, Japan Laboratory of Emerging Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto 1 21 24, Kagoshima 890 0065, Japan Electronic address
    Vet Parasitol 201:169-75. 2014
    ..Taken together, our findings raise the new possibility that HlATG genes play distinct biological roles in eggs, unfed ticks, engorged ticks (metamorphosis), and in each organ. ..
  76. ncbi request reprint Beneficial effect of hyperbaric oxygen therapy on the follicular survival in the mouse ovary after transplantation
    Satomi Hayashi
    Research Unit for Functional Genomics, National Research Center of Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 8555, Japan
    J Reprod Dev 58:260-3. 2012
    ..These results indicate HBO can be effectively used for the enhancement of survival of transplanted ovarian tissues...
  77. doi request reprint Exposure to 50 Hz electric fields reduces stress-induced glucocorticoid levels in BALB/c mice in a kV/m- and duration-dependent manner
    Takuya Hori
    Bio Self Regulating Science Laboratory, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan Hakuju Institute for Health Science, Tokyo, Japan
    Bioelectromagnetics 36:302-8. 2015
    ..Therefore, our results demonstrate that extremely low-frequency EFs alter stress response of mice in a kV/m- and duration-dependent manner...
  78. doi request reprint Fertilizability, developmental competence, and chromosomal integrity of oocytes microinjected with pre-treated spermatozoa in mice
    Hiroyuki Watanabe
    Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Reproduction 139:513-21. 2010
    ..These results indicate that excessive sperm pre-treatment such as DTT for 60 min generates severe chromosome damage in injected oocytes, and that the damage decreases developmental competence to live fetuses but not to blastocysts...
  79. pmc Loop-mediated isothermal amplification for detection of African trypanosomes
    Noritaka Kuboki
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Clin Microbiol 41:5517-24. 2003
    ..In vivo studies in mice infected with human-infective T. brucei gambiense further highlight the potential clinical importance of LAMP as a diagnostic tool for the identification of African trypanosomiasis...
  80. ncbi request reprint Preparation of quinoline hexose analogs as novel chloroquine-resistant malaria treatments (1). Synthesis of 4-hydroxyquinoline-beta-glucosides
    Hiroshi Suzuki
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 0555, Japan
    Chem Pharm Bull (Tokyo) 55:821-4. 2007
    ..Quinoline hexose analogs are expected to be useful as novel agents for treatment of chloroquine-resistant malaria. Here, we report preparation of 4-hydroxy quinoline-beta-glucosides from anilines in 4 steps...
  81. doi request reprint Detection of sex chromosome aneuploidy in dog spermatozoa by triple color fluorescence in situ hybridization
    Haruna Komaki
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
    Theriogenology 82:652-6. 2014
    ..127% (ranged between 0% and 0.316%). This percentage of canine sex chromosome aneuploidy was lower than the one reported in cattle, horses, river buffalo, and goats sperm, but higher than that observed in mice and sheep. ..
  82. ncbi request reprint Exposure of C57BL/6J male mice to an electric field improves copulation rates with superovulated females
    Takuya Hori
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Japan
    J Reprod Dev 51:393-7. 2005
    ..05). These results suggest that the exposure of C57BL/6J male mice to an electric field improves their sub-fertility activity in mating with superovulated females...
  83. ncbi request reprint Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 131:129-36. 2003
    ..gibsoni in dogs...
  84. ncbi request reprint Phenotype and function of murine peritoneal cavity macrophage derived-dendritic cells
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Hokkaido, Japan
    J Vet Med Sci 64:813-20. 2002
    ..The generated DC cells efficiently presented soluble protein antigen to CD3(+) spleen T cells...
  85. ncbi request reprint Lipid raft microdomains mediate class A scavenger receptor-dependent infection of Brucella abortus
    Suk Kim
    Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro shi, Hokkaido 080 8555, Japan
    Microb Pathog 37:11-9. 2004
    ..abortus regulates signal transduction dependent on SR-A to form replicative phagosomes, and which is mediated by lipid rafts...