T Mizutani

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. pmc Novel betaherpesvirus in bats
    Shumpei Watanabe
    The University of Tokyo, Tokyo, Japan
    Emerg Infect Dis 16:986-8. 2010
  2. pmc A seroepidemiologic study of Reston ebolavirus in swine in the Philippines
    Yusuke Sayama
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, 208 0011, Japan
    BMC Vet Res 8:82. 2012
  3. doi request reprint Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica
    Tetsuya Mizutani
    Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo, Japan
    Virology 412:179-87. 2011
  4. ncbi request reprint Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Virology 327:169-74. 2004
  5. ncbi request reprint [Current topics on SARS coronavirus]
    Tetsuya Mizutani
    Virology 1, Gakuen 4 7 1, Musashimurayama City, Tokyo 208 0011, Japan
    Uirusu 54:97-105. 2004
  6. ncbi request reprint Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    FEBS Lett 577:187-92. 2004
  7. ncbi request reprint JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Biochim Biophys Acta 1741:4-10. 2005
  8. ncbi request reprint Mechanisms of establishment of persistent SARS-CoV-infected cells
    Tetsuya Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Biochem Biophys Res Commun 347:261-5. 2006
  9. ncbi request reprint Regulation of p90RSK phosphorylation by SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    FEBS Lett 580:1417-24. 2006
  10. ncbi request reprint Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    FEMS Immunol Med Microbiol 46:236-43. 2006

Detail Information

Publications75

  1. pmc Novel betaherpesvirus in bats
    Shumpei Watanabe
    The University of Tokyo, Tokyo, Japan
    Emerg Infect Dis 16:986-8. 2010
    ..Using a modified rapid determination system for viral RNA/DNA sequences, we identified a novel bat betaherpesvirus 2 not detected by herpesvirus consensus PCR. This modified system is useful for detecting unknown viruses...
  2. pmc A seroepidemiologic study of Reston ebolavirus in swine in the Philippines
    Yusuke Sayama
    Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo, 208 0011, Japan
    BMC Vet Res 8:82. 2012
    ..In late 2008, REBOV infections were identified in swine for the first time in the Philippines...
  3. doi request reprint Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica
    Tetsuya Mizutani
    Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo, Japan
    Virology 412:179-87. 2011
    ..JEECV was detected in both naturally and experimentally infected eels, suggesting that JEECV potentially causes VECNE...
  4. ncbi request reprint Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Virology 327:169-74. 2004
    ..PKCzeta, which is downstream of the PI3K pathway, was also phosphorylated in virus-infected cells. These results suggested that weak activation of Akt cannot prevent apoptosis induced by SARS-CoV infection in Vero E6 cells...
  5. ncbi request reprint [Current topics on SARS coronavirus]
    Tetsuya Mizutani
    Virology 1, Gakuen 4 7 1, Musashimurayama City, Tokyo 208 0011, Japan
    Uirusu 54:97-105. 2004
    ..Topics of the research on the present SARS-research field are development of vaccine, inspecting of medicine and establishment of diagnostic method. Here, the newest information is offered about these researches...
  6. ncbi request reprint Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    FEBS Lett 577:187-92. 2004
    ..As STAT3 acts as an activator of transcription in the nucleus, these results suggest that STAT3 lacks its activity on transcription in SARS-CoV-infected Vero E6 cells...
  7. ncbi request reprint JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208 0011, Japan
    Biochim Biophys Acta 1741:4-10. 2005
    ..These results indicated that two signaling pathways of JNK and PI3K/Akt were important for the establishment of persistence in Vero E6 cells...
  8. ncbi request reprint Mechanisms of establishment of persistent SARS-CoV-infected cells
    Tetsuya Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Biochem Biophys Res Commun 347:261-5. 2006
    ..These results indicated that at least four proteins, Akt, JNK, Bcl-2 and Bcl-xL, are necessary for survival of persistently SARS-CoV-infected cells...
  9. ncbi request reprint Regulation of p90RSK phosphorylation by SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    FEBS Lett 580:1417-24. 2006
    ..These results indicated that phosphorylation status of p90RSK by SARS-CoV infection is different from that by stimulation of EGF. This is the first detailed report regarding regulation of p90RSK phosphorylation by virus infection...
  10. ncbi request reprint Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    FEMS Immunol Med Microbiol 46:236-43. 2006
    ..This is the first study to analyze SARS-CoV-induced cell growth inhibition...
  11. ncbi request reprint Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans
    T Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Arch Virol 152:1019-25. 2007
    ..fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis...
  12. pmc Rapid genome sequencing of RNA viruses
    Tetsuya Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama City, Tokyo, Japan
    Emerg Infect Dis 13:322-4. 2007
    ..This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses...
  13. ncbi request reprint [Establishment of a system for determination of emerging infectious diseases (RDV method), and its application]
    Tetsuya Mizutani
    Virology 1, National Institute of Infectious Diseases, Gakuen4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    Uirusu 57:217-25. 2007
    ..We demonstrated the clinical application of this system in human specimens and its diagnostic usage in mosquitoes. Furthermore, new adenovirus and herpesvirus were found in bats using the RDV method...
  14. ncbi request reprint Phosphorylation of p38 MAPK and its downstream targets in SARS coronavirus-infected cells
    Tetsuya Mizutani
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    Biochem Biophys Res Commun 319:1228-34. 2004
    ..The p38 MAPK inhibitor, SB203580, inhibited effectively phosphorylation of HSP-27, CREB, and eIF4E in SARS-CoV-infected cells. However, viral protein synthesis was not affected by treatment of SB203580...
  15. ncbi request reprint Signal transduction in SARS-CoV-infected cells
    T Mizutani
    Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo, Japan
    Ann N Y Acad Sci 1102:86-95. 2007
    ..This review highlights recent progress regarding SARS-CoV biology, especially signal transduction in SARS-CoV-infected cells...
  16. ncbi request reprint An improved procedure for rapid determination of viral RNA sequences of avian RNA viruses
    K Sakai
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Arch Virol 152:1763-5. 2007
    ..A system for rapid determination of viral RNA sequences, RDV, was improved for detection of avian RNA virus in allantoic fluids. We detected avian paramyxovirus nucleotide sequences using RDV method ver 2.0...
  17. ncbi request reprint Highly attenuated vaccinia virus DIs as a potential SARS vaccine
    Koji Ishii
    National Institute of Infectious Diseases, Tokyo, Japan
    Adv Exp Med Biol 581:593-6. 2006
  18. ncbi request reprint Induction of protective immunity against severe acute respiratory syndrome coronavirus (SARS-CoV) infection using highly attenuated recombinant vaccinia virus DIs
    Koji Ishii
    Department of Virology II, National Institute of Infectious Diseases, Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Virology 351:368-80. 2006
    ..Thus, replication-deficient DIs constructs hold promise for the development of a safe and potent SARS vaccine...
  19. ncbi request reprint Characterization of persistent SARS-CoV infection in Vero E6 cells
    Tetsuya Mizutani
    National Institute of Infectious Diseases, Tokyo, Japan
    Adv Exp Med Biol 581:323-6. 2006
  20. pmc Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
    Mina Nakauchi
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan
    Clin Vaccine Immunol 16:1132-8. 2009
    ..The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus...
  21. ncbi request reprint Inhibitory effect of mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Antiviral Res 66:159-63. 2005
    ..Mizoribine inhibited replication of SARS-CoV more strongly than ribavirin. However, neither drug could completely inhibit replication of SARS-CoV even at concentrations up to 100 microg/ml...
  22. ncbi request reprint Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein
    Shuetsu Fukushi
    Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Gen Virol 86:2269-74. 2005
    ..VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection...
  23. ncbi request reprint Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama 208 0011, Tokyo, Japan
    J Virol Methods 125:181-6. 2005
    ..The SARS-CoV rNP-based IgG ELISA has an advantage over the conventional IgG ELISA in that the antigen can be prepared by laboratory workers without the risk of infection...
  24. ncbi request reprint Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 59:323-5. 2006
    ..These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF...
  25. ncbi request reprint Evaluation of a novel vesicular stomatitis virus pseudotype-based assay for detection of neutralizing antibody responses to SARS-CoV
    Shuetsu Fukushi
    Department of Virology I, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 78:1509-12. 2006
    ..The newly developed neutralization assay was more sensitive to low antibody titers in serum samples. Thus, the VSV-SARS-St19 is a useful tool for detecting Nabs against SARS-CoV...
  26. doi request reprint Virulence and pathophysiology of the Congo Basin and West African strains of monkeypox virus in non-human primates
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases NIID, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Gen Virol 90:2266-71. 2009
    ..Zr-599 affected respiratory, genito-urinary and gastrointestinal tract organs more severely than Liberia. Zr-599 was more virulent than Liberia and one of the factors might be the difference in organ tropism...
  27. ncbi request reprint Diagnosis and assessment of monkeypox virus (MPXV) infection by quantitative PCR assay: differentiation of Congo Basin and West African MPXV strains
    Masayuki Saijo
    Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208 0011, Japan
    Jpn J Infect Dis 61:140-2. 2008
    ..It was possible to differentiate between these two groups with the LC-qPCR. Thus, the newly developed LC-qPCR is a useful and reliable diagnostic tool for MPXV infection...
  28. pmc Amino acid substitutions in the s2 region enhance severe acute respiratory syndrome coronavirus infectivity in rat angiotensin-converting enzyme 2-expressing cells
    Shuetsu Fukushi
    Special Pathogens Laboratory, Department of Virology I, Center for Pathogens Genomics, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 81:10831-4. 2007
    ..Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region...
  29. pmc Development of recombinant nucleoprotein-based diagnostic systems for Lassa fever
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Clin Vaccine Immunol 14:1182-9. 2007
    ..These LASV rNP-based diagnostics were confirmed to be useful in the diagnosis of LF even in institutes without a high containment laboratory, since the antigens can be prepared without manipulation of the infectious viruses...
  30. pmc LC16m8, a highly attenuated vaccinia virus vaccine lacking expression of the membrane protein B5R, protects monkeys from monkeypox
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 80:5179-88. 2006
    ..These results indicate that LC16m8 prevents lethal monkeypox in monkeys, and they suggest that LC16m8 may induce protective immunity against smallpox...
  31. doi request reprint Identification and molecular characterization of a new nonsegmented double-stranded RNA virus isolated from Culex mosquitoes in Japan
    Haruhiko Isawa
    Department of Medical Entomology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Virus Res 155:147-55. 2011
    ..These results indicated that OMRV is a new member of the family of nonsegmented dsRNA viruses infecting arthropod hosts, but not fungal or protozoan hosts...
  32. ncbi request reprint Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro
    N Kato
    Virology Division, National Cancer Center Research Institute, Tokyo, Japan
    J Gen Virol 79:1859-69. 1998
    ..Following a comparison of the sequences, 11 amino acids were identified as candidates for determinants of the cell tropism of HCV...
  33. doi request reprint Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections
    Itoe Iizuka
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    J Med Virol 81:1102-8. 2009
    ..The LAMP assays made it possible to discriminate between Congo Basin and West African MPXV. The LAMP developed in this study is useful not only for diagnosis of but also for the assessment of MPXV infections...
  34. ncbi request reprint Pseudotyped vesicular stomatitis virus for functional analysis of SARS coronavirus spike protein
    Shuetsu Fukushi
    National Institute of Infectious Diseases, Tokyo, Japan
    Adv Exp Med Biol 581:293-6. 2006
  35. ncbi request reprint SWI/SNF complex is essential for NRSF-mediated suppression of neuronal genes in human nonsmall cell lung carcinoma cell lines
    H Watanabe
    1Division of Host Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Nagasaki, Tokyo, Japan
    Oncogene 25:470-9. 2006
    ....
  36. pmc Bat coronaviruses and experimental infection of bats, the Philippines
    Shumpei Watanabe
    University of Tokyo, Tokyo, Japan
    Emerg Infect Dis 16:1217-23. 2010
    ..After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection...
  37. ncbi request reprint The effect of a glucocorticoid on olfactory response of isolated mouse olfactory cells
    Kiyoaki Kamakazu
    Department of Otorhinolaryngology, Showa University School of Medicine, 1 5 8 Hatanodai, Shinagawaku, Tokyo, 142 8666, Japan
    In Vivo 17:343-7. 2003
    ..The inhibitory effect of betamethasone on Ca2+ influx into olfactory cells was attenuated by pre-treatment olfactory cells with RU486, a glucocorticoid receptor antagonist...
  38. doi request reprint Novel reovirus isolation from an Ostrich (Struthio camelus) in Japan
    Kouji Sakai
    Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    Vet Microbiol 134:227-32. 2009
    ..To our knowledge, this is the first genetic report of an orthoreovirus isolated from an ostrich...
  39. ncbi request reprint The rat enhancer of split- and hairy-related protein-2 gene: hepatic expression, genomic structure, and promoter analysis
    Satoko Hirano
    Department of Pediatrics, Fukui Medical University, 910 1193 Fukui, Japan
    Arch Biochem Biophys 422:81-90. 2004
    ..Therefore, other E box-binding transcription factors differing from USF proteins or USF-associated proteins are necessary for transcriptional stimulation of the rat SHARP-2 gene...
  40. ncbi request reprint Cloning and characterization of granulosa cell high-mobility group (HMG)-box protein-1, a novel HMG-box transcriptional regulator strongly expressed in rat ovarian granulosa cells
    Takashi Kajitani
    Department of Biochemistry, Fukui Medical University, Matsuoka, Fukui, 910 1193, Japan
    Endocrinology 145:2307-18. 2004
    ....
  41. ncbi request reprint Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome
    Kumari Lokugamage
    Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060 0818, Japan
    Virus Res 101:127-34. 2004
    ..Therefore, we suggest that AMR virus may constitute a distinct serotype within the genus Hantavirus...
  42. pmc Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells
    Sangeeta Banerjee
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1019, USA
    J Virol 76:5937-48. 2002
    ..Enhancement of virus-specific protein synthesis through virus-induced eIF4E activation has not been reported in any other viruses...
  43. ncbi request reprint Rat zinc-fingers and homeoboxes 1 (ZHX1), a nuclear factor-YA-interacting nuclear protein, forms a homodimer
    Satoko Hirano
    Department of Pediatrics, Fukui Medical University, Fukui 910 1193, Japan
    Gene 290:107-14. 2002
    ..Thus, we report on the molecular cloning, expression and characterization of full-length rat ZHX1 cDNA...
  44. ncbi request reprint Synthesis and biological evaluation of nucleoside analogues having 6-chloropurine as anti-SARS-CoV agents
    Masahiro Ikejiri
    Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314 1 Shido, Sanuki, Kagawa 769 2193, Japan
    Bioorg Med Chem Lett 17:2470-3. 2007
    ..Moreover, we observed several SAR trends such as the antiviral effects of the 6-chloropurine moiety, unprotected 5'-hydroxyl group and benzoylated 5'-hydroxyl group...
  45. ncbi request reprint Early growth response gene-1 regulates the expression of the rat luteinizing hormone receptor gene
    Miki Yoshino
    Department of Biochemistry, Fukui Medical University, Shimoaizuki, Matsuoka, Fukui 910, Japan
    Biol Reprod 66:1813-9. 2002
    ..The present study provides further support to the hypothesis that Egr-1 plays important roles in the pituitary-gonadal axis...
  46. doi request reprint Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)
    Shumpei Watanabe
    Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Clin Virol 43:56-9. 2008
    ..However, some species of viruses, including the Yokose virus (YOKV), a flavivirus, could not be detected using the conventional procedures...
  47. ncbi request reprint Enzyme-linked immunosorbent assay using recombinant antigens expressed in mammalian cells for serodiagnosis of tick-borne encephalitis
    Kentarou Yoshii
    Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Virol Methods 108:171-9. 2003
    ..These observations indicated that this newly developed ELISA system could distinguish tick-borne encephalitis from Japanese encephalitis infection, and that it constitutes a useful and safe alternative to conventional ELISA systems...
  48. ncbi request reprint Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of tick-borne encephalitis virus
    Daisuke Hayasaka
    Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    J Gen Virol 85:1007-18. 2004
    ..Comparative neurovirulence tests revealed how the accumulation of individual mutations (E-S(40)-->P, NS5-V(378)-->A and NS5-R(674)-->K) can result in the attenuation of a virus...
  49. ncbi request reprint The mouse zinc-fingers and homeoboxes (ZHX) family; ZHX2 forms a heterodimer with ZHX3
    Hiroko Kawata
    CREST, JST Japan Science and Technology, 910 1193, Fukui, Japan
    Gene 323:133-40. 2003
    ..5-, 6.5- and 4.4-kb, are ubiquitously expressed, although their levels vary. Lastly, in vitro and in vivo protein-protein interaction assays revealed that ZHX2 is able to form a heterodimer with ZHX3 via a region containing each HD1...
  50. ncbi request reprint Development of an efficient method for recovery of Puumala and Puumala-related viruses by inoculation of Mongolian gerbils
    Nandadeva Lokugamage
    Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    J Vet Med Sci 65:1189-94. 2003
    ..These findings indicate that newborn MG inoculation is an efficient method to recover PUU and PUU-related viruses...
  51. ncbi request reprint Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and far-eastern Russia
    Akiko Goto
    Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060 0818, Japan
    Jpn J Vet Res 49:297-307. 2002
    ..Genetic analysis revealed 1.4% of amino acids to differ with Sofjin-HO. No difference between the two strains was detected in the motif sequence of the viral enzyme, cleavage sites of viral protein or glycosylation sites of NS1...
  52. ncbi request reprint A BHK-21 cell culture-adapted tick-borne encephalitis virus mutant is attenuated for neuroinvasiveness
    Akiko Goto
    Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita ku, Sapporo 060 0818, Japan
    Vaccine 21:4043-51. 2003
    ..The attenuation process involved a single amino acid change in the E protein, which may have resulted in the rapid clearance of the virus due to its high affinity for negatively charged molecules in vivo...
  53. ncbi request reprint Discrimination of West Nile virus and Japanese encephalitis virus strains using RT-PCR RFLP analysis
    Kazuya Shirato
    Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060 0818, Japan
    Microbiol Immunol 47:439-45. 2003
    ..Our method is useful in distinguishing WN viruses from the endemic background of JE viruses, and in discriminating the highly virulent WN strain, which was isolated in New York in 1999, from other WN virus strains...
  54. pmc Genetic characterization of hantaviruses transmitted by the Korean field mouse (Apodemus peninsulae), Far East Russia
    Kumari Lokugamage
    Hokkaido University, Sapporo, Japan
    Emerg Infect Dis 8:768-76. 2002
    ..Our findings suggest that the Korean field mouse (A. peninsulae) is the reservoir for a hantavirus that causes HFRS over a vast area of east Asia, including Far East Russia...
  55. ncbi request reprint Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model
    Kumari Lokugamage
    Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Jpn J Vet Res 51:143-9. 2004
    ..These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice...
  56. ncbi request reprint Identification of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible and -suppressive genes in the rat placenta: induction of interferon-regulated genes with possible inhibitory roles for angiogenesis in the placenta
    Tetsuya Mizutani
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Matsuoka, Fukui 910 1193, Japan
    Endocr J 51:569-77. 2004
    ....
  57. pmc Activation of the lutropin/choriogonadotropin receptor in MA-10 cells leads to the tyrosine phosphorylation of the focal adhesion kinase by a pathway that involves Src family kinases
    Tetsuya Mizutani
    Department of Pharmacology, 2 319B BSB, 51 Newton Road, The University of Iowa, Iowa City, Iowa 52242 1109, USA
    Mol Endocrinol 20:619-30. 2006
    ....
  58. ncbi request reprint Transcriptional regulation of the epiregulin gene in the rat ovary
    Toshio Sekiguchi
    Department of Biochemistry, Fukui Medical University, Shimoaizuki, Matsuoka, Fukui 910 1193, Japan
    Endocrinology 143:4718-29. 2002
    ..The restricted and hormonally regulated expression of epiregulin in the rat ovarian granulosa cells may correspond to the physiological relevance of this peptide growth factor to the FSH-induced ovarian follicular growth and maturation...
  59. ncbi request reprint Development of an enzyme-linked immunosorbent assay for serological diagnosis of tick-borne encephalitis using subviral particles
    Mayumi Obara
    Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Virol Methods 134:55-60. 2006
    ..The SP-IgG and SP-IgM ELISAs showed no cross-reactivity with antibodies to the JE virus. The results indicate that these ELISAs will be useful for the detection of TBE-specific antibodies...
  60. ncbi request reprint Involvement of cyclic adenosine 5'-monophosphate response element-binding protein, steroidogenic factor 1, and Dax-1 in the regulation of gonadotropin-inducible ovarian transcription factor 1 gene expression by follicle-stimulating hormone in ovarian gran
    Takashi Yazawa
    Department of Biochemistry, Fukui Medical University, Fukui 910 1193, Japan
    Endocrinology 144:1920-30. 2003
    ..These results suggest that the synergistic action of CRE binding protein and SF-1 and the rapid down-regulation of Dax-1 are responsible for the acute induction of GIOT1 gene by gonadotropin...
  61. ncbi request reprint Differentiation of adult stem cells derived from bone marrow stroma into Leydig or adrenocortical cells
    Takashi Yazawa
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Matsuoka cho, Fukui 910 1193, Japan
    Endocrinology 147:4104-11. 2006
    ..These results indicate that MSCs represent a useful source of stem cells for producing steroidogenic cells that may provide basis for their use in cell and gene therapy...
  62. pmc Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription
    Daiji Endoh
    Laboratory of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University Ebetsu 069 8501, Japan
    Nucleic Acids Res 33:e65. 2005
    ..The results suggest that cDNA RDA with non-ribosomal primers can be used for species-independent detection of viruses, including new viruses...
  63. ncbi request reprint Insulin induces the expression of the SHARP-2/Stra13/DEC1 gene via a phosphoinositide 3-kinase pathway
    Kazuya Yamada
    Department of Biochemistry, Fukui Medical University, Japan
    J Biol Chem 278:30719-24. 2003
    ..In addition, nuclear run-on assay revealed that transcription of the rat SHARP-2 gene was induced by insulin. Thus, we conclude that insulin induces the transcription of the rat SHARP-2 gene via a phosphoinositide 3-kinase pathway...
  64. ncbi request reprint Genomic structure and analysis of transcriptional regulation of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene
    Zhangfei Shou
    Department of Biochemistry, Fukui Medical University, 910 1193, Fukui, Japan
    Gene 302:83-94. 2003
    ..Thus, we conclude that PEA3 and YY1 synergistically stimulate the transcription of the ZHX1 gene...
  65. ncbi request reprint Different chemokine expression in lethal and non-lethal murine West Nile virus infection
    Kazuya Shirato
    Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan
    J Med Virol 74:507-13. 2004
    ..By contrast, BLC mRNA was not detected in either group of mice, and BMAC mRNA was highly up-regulated in late stage of infection with the non-lethal Eg101 strain relative to levels in NY strain-infected mice...
  66. ncbi request reprint Role of the N-linked glycans of the prM and E envelope proteins in tick-borne encephalitis virus particle secretion
    Akiko Goto
    Laboratory of Public Health, Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita ku, Sapporo 060 0818, Japan
    Vaccine 23:3043-52. 2005
    ..These data suggest that the glycan associated with the N-linked glycosylation site at position 154 in protein E plays an important role in VLP secretion...
  67. ncbi request reprint Epizootiological and epidemiological study of hantavirus infection in Japan
    Nandadeva Lokugamage
    Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Japan
    Microbiol Immunol 48:843-51. 2004
    ..rattus, and C. rufocanus carry hantaviruses as the reservoir animals in Japan. Infected R. rattus and R. norvegicus in port areas could be the sources of human SEOV infection and a threat to travelers and individuals working in seaports...
  68. pmc Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor
    Hiroko Kawata
    Core Research for Evolutional Science and Technology CREST, Japan Science and Technology JST, Fukui 910 1193, Japan
    Biochem J 373:747-57. 2003
    ..Thus the ZHX family of proteins may participate in the expression of a number of NF-Y-regulated genes via a more organized transcription network...
  69. ncbi request reprint Functional analysis and the molecular dissection of zinc-fingers and homeoboxes 1 (ZHX1)
    Kazuya Yamada
    Department of Biochemistry, Fukui Medical University, Japan
    Biochem Biophys Res Commun 297:368-74. 2002
    ..The acidic region corresponding to the amino acid sequence between 831 and 873 was a repressor domain and dimerization was prerequisited for full repressor activity...
  70. ncbi request reprint Effects of ovarian theca cells on granulosa cell differentiation during gonadotropin-independent follicular growth in cattle
    Makoto Orisaka
    Department of Obstetrics and Gynecology, University of Fukui, Fukui, Japan
    Mol Reprod Dev 73:737-44. 2006
    ..e., androgen substrate production and aromatase regulation, and that theca cell-derived factors regulate estradiol and progesterone production in a way that reflects steroidogenesis during the follicular phase of the estrous cycle...
  71. pmc Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3
    Kazuya Yamada
    Department of Biochemistry, Fukui Medical University, and Japan and CREST, Japan Science and Technology, Fukui 910 1193, Japan
    Biochem J 373:167-78. 2003
    ....
  72. pmc Co-activator p120 is increased by gonadotropins in the rat ovary and enhances progesterone receptor activity
    Miki Yoshino
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Shimoaizuki, Matsuoka, Fukui 910 1193, Japan
    Reprod Biol Endocrinol 4:50. 2006
    ..In this study we identify rat homologue of p120, a nuclear transcription co-activator, as one of the FSH inducible genes in the rat granulosa cells...
  73. ncbi request reprint Granulosa cells promote differentiation of cortical stromal cells into theca cells in the bovine ovary
    Makoto Orisaka
    Department of Obstetrics and Gynecology, University of Fukui, Fukui 910 1193, Japan
    Biol Reprod 75:734-40. 2006
    ..We suggest that GC-S(C) interaction is important in the formation of the TC layer during early follicular development, although the nature of this interaction remains to be determined...
  74. doi request reprint Regulation of NGFI-B/Nur77 gene expression in the rat ovary and in leydig tumor cells MA-10
    Yoshihiko Inaoka
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui, Japan
    Mol Reprod Dev 75:931-9. 2008
    ..The rapid induction of NGFI-B/Nur77 gene expression by LH/hCG in MA-10 cells appears to be mediated by both CREB and Jun family proteins through the cAMP-protein kinase A (PKA) pathway...
  75. ncbi request reprint Rapid determination of viral RNA sequences in mosquitoes collected in the field
    Yuki Kihara
    Faculty of Pharmaceutical Sciences, Fukuoka University, 8 19 1 Nanakuma, Jonan ku, Fukuoka 814 0180, Japan
    J Virol Methods 146:372-4. 2007
    ..Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses...