Nobuo Koizumi

Summary

Affiliation: National Institute of Infectious Diseases
Country: Japan

Publications

  1. doi request reprint Molecular and serological investigation of Leptospira and leptospirosis in dogs in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 62:630-6. 2013
  2. pmc A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan
    J Clin Microbiol 50:2072-4. 2012
  3. ncbi request reprint Investigation of reservoir animals of Leptospira in the northern part of Miyazaki Prefecture
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
    Jpn J Infect Dis 61:465-8. 2008
  4. doi request reprint Human leptospirosis cases and the prevalence of rats harbouring Leptospira interrogans in urban areas of Tokyo, Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 58:1227-30. 2009
  5. ncbi request reprint Prevalence of Leptospira spp. in the kidneys of wild boars and deer in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    J Vet Med Sci 71:797-9. 2009
  6. ncbi request reprint Leptospiral immunoglobulin-like proteins elicit protective immunity
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Vaccine 22:1545-52. 2004
  7. ncbi request reprint Serological and genetic analysis of leptospirosis in patients with acute febrile illness in kandy, sri lanka
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Jpn J Infect Dis 62:474-5. 2009
  8. ncbi request reprint Isolation and characterization of Leptospira spp. from raccoons in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases
    J Vet Med Sci 71:425-9. 2009
  9. pmc The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlA
    Ken Shimuta
    Department of Bacteriology I, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    BMC Microbiol 9:261. 2009
  10. pmc Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines
    Sharon Y A M Villanueva
    Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Am J Trop Med Hyg 82:889-98. 2010

Collaborators

Detail Information

Publications30

  1. doi request reprint Molecular and serological investigation of Leptospira and leptospirosis in dogs in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 62:630-6. 2013
    ..This study suggests that the virulence of members of serogroup Hebdomadis in dogs may be associated with the genotypes in this serogroup...
  2. pmc A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan
    J Clin Microbiol 50:2072-4. 2012
    ..in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR...
  3. ncbi request reprint Investigation of reservoir animals of Leptospira in the northern part of Miyazaki Prefecture
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan
    Jpn J Infect Dis 61:465-8. 2008
    ..Anti-Leptospira antibodies were also detected among symptomatic hound dogs. These results suggest that these feral animals may cause leptospirosis and pose a potential risk to hunters and workers in the meat processing industry...
  4. doi request reprint Human leptospirosis cases and the prevalence of rats harbouring Leptospira interrogans in urban areas of Tokyo, Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 58:1227-30. 2009
    ..Antibodies against these serovars and their DNA were detected in the patients. This suggests that rats are important reservoirs of leptospirosis, and that rat-borne leptospires occur in urban areas of Tokyo...
  5. ncbi request reprint Prevalence of Leptospira spp. in the kidneys of wild boars and deer in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    J Vet Med Sci 71:797-9. 2009
    ..45). The Leptospira species harbored by these animals were deduced to be L. interrogans (from 22 animals) and L. borgpetersenii (from 1 animal)...
  6. ncbi request reprint Leptospiral immunoglobulin-like proteins elicit protective immunity
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Vaccine 22:1545-52. 2004
    ..Furthermore, patients' sera infected with heterologous serovars' strains contained antibodies against LigA-m and LigB-m. Our results suggest that LigA-m and LigB-m proteins are new vaccine candidates for the prevention of leptospirosis...
  7. ncbi request reprint Serological and genetic analysis of leptospirosis in patients with acute febrile illness in kandy, sri lanka
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162 8640, Japan
    Jpn J Infect Dis 62:474-5. 2009
    ..kirschneri (2 and 1 samples, respectively). These results confirm the existence of a wide array of leptospiral species and serogroups in Sri Lanka and would help to thoroughly elucidate the epidemiology of leptospirosis in this country...
  8. ncbi request reprint Isolation and characterization of Leptospira spp. from raccoons in Japan
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases
    J Vet Med Sci 71:425-9. 2009
    ..Although it is unclear if the raccoons carried leptospires in their kidneys at the time imported, there is no doubt that imported animals are a new reservoir animal of leptospires in Japan...
  9. pmc The hemolytic and cytolytic activities of Serratia marcescens phospholipase A (PhlA) depend on lysophospholipid production by PhlA
    Ken Shimuta
    Department of Bacteriology I, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    BMC Microbiol 9:261. 2009
    ..There have been few studies of the virulence factors of this bacterium. The only S. marcescens hemolytic and cytotoxic factor reported, thus far, is the hemolysin ShlA...
  10. pmc Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines
    Sharon Y A M Villanueva
    Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Am J Trop Med Hyg 82:889-98. 2010
    ..Results showed that these four Leptospira serovars and serogroups are circulating among rats, and that these animals may be one of the possible transmission sources of leptospirosis in the Philippines...
  11. doi request reprint Detection of Streptobacillus spp. in feral rats by specific polymerase chain reaction
    Masanobu Kimura
    Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan
    Microbiol Immunol 52:9-15. 2008
    ..rattus (30/52). The nucleotide sequence analysis of the 16S rRNA gene of Streptobacillus spp. isolated from oral swabs of feral rats showed at least two different types of bacteria among isolates from R. norvegicus and R. rattus...
  12. ncbi request reprint Identification of a novel antigen of pathogenic Leptospira spp. that reacted with convalescent mice sera
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Med Microbiol 52:585-9. 2003
    ..The gene for LAg42 is conserved among pathogenic leptospires but not among non-pathogenic leptospires, which suggests its involvement in virulence...
  13. doi request reprint Diagnosis of acute leptospirosis
    Takao Toyokawa
    Field Epidemiology Training Program Japan, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
    Expert Rev Anti Infect Ther 9:111-21. 2011
    ..will accelerate our understanding of Leptospira pathogenesis and provide insights into the development of more efficient and accurate diagnostic tests for acute-phase leptospirosis...
  14. pmc Development of immunochromatography-based methods for detection of leptospiral lipopolysaccharide antigen in urine
    Dian Widiyanti
    Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Clin Vaccine Immunol 20:683-90. 2013
    ..In summary, this ICG-based LFA can be used as an alternative diagnostic assay for leptospirosis. Further development is still necessary to improve the assay...
  15. ncbi request reprint [Diagnostic tests: Leptospira]
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases
    Nihon Rinsho 63:214-6. 2005
  16. pmc The GrlR-GrlA regulatory system coordinately controls the expression of flagellar and LEE-encoded type III protein secretion systems in enterohemorrhagic Escherichia coli
    Sunao Iyoda
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    J Bacteriol 188:5682-92. 2006
    ..The fact that an EHEC strain constitutively expressing FlhD/FlhC cannot adhere to HeLa cells leads us to hypothesize that GrlA-dependent repression of the flagellar regulon is important for efficient cell adhesion of EHEC to host cells...
  17. ncbi request reprint Molecular cloning and characterization of a novel leptospiral lipoprotein with OmpA domain
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    FEMS Microbiol Lett 226:215-9. 2003
    ..Thus, Loa22 may be a candidate for a novel vaccine against infection with pathogenic leptospires...
  18. doi request reprint Multiple-locus variable-number tandem repeat analysis of Leptospira interrogans and Leptospira borgpetersenii isolated from small feral and wild mammals in East Asia
    Nobuo Koizumi
    Department of Bacteriology I, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan Electronic address
    Infect Genet Evol 36:434-40. 2015
    ..This study demonstrates that there may be variability in the range of genetic diversity among different Leptospira serogroups, which may be attributed to maintenance host animals and environmental factors. ..
  19. ncbi request reprint Probable laboratory contamination of clinical specimens with Leptospira meyeri
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    Microbiol Immunol 47:305-6. 2003
    ..However, intraperitoneal inoculation of the isolate (NIID1) failed to kill hamsters and none of the patients' sera reacted with L. meyeri strains isolated, indicating leptospiral contamination occurred during laboratory investigation...
  20. ncbi request reprint [A case of leptospirosis diagnosed early by flaB-PCR]
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases, Tokai University School of Medicine
    Kansenshogaku Zasshi 77:627-30. 2003
    ..Seroconversion occurred at hospital day 6 and Leptospira was isolated from the patient's blood at hospital day 16, demonstrating that flaB-PCR method was very useful in early diagnosis of leptospirosis...
  21. doi request reprint Multiple-locus variable-number tandem repeat analysis and clinical characterization of Leptospira interrogans canine isolates
    Nobuo Koizumi
    Department of Bacteriology I, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    J Med Microbiol 64:288-94. 2015
    ..Our results also suggest that the virulence mechanisms and target organs in dogs may differ by Leptospira genotype. ..
  22. pmc Identification of a novel adhesion molecule involved in the virulence of Legionella pneumophila
    Bin Chang
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Infect Immun 73:4272-80. 2005
    ..Thus, we conclude that the laiA gene encodes a virulence factor that is involved in transmission of L. pneumophila 80-045 and may play a role in Legionnaires' disease in humans...
  23. ncbi request reprint [Leptospirosis]
    Nobuo Koizumi
    Department of Bacteriology, National Institute of Infectious Diseases
    Nihon Rinsho 65:200-3. 2007
  24. pmc Comparison of Bacterial Burden and Cytokine Gene Expression in Golden Hamsters in Early Phase of Infection with Two Different Strains of Leptospira interrogans
    Rie Fujita
    Graduate School of Bio Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan
    PLoS ONE 10:e0132694. 2015
    ..In addition, our results suggest a serovar Manilae-specific mechanism responsible for inducing severe damage in kidneys and hemorrhage in lung. ..
  25. ncbi request reprint Sporadic cases and an outbreak of leptospirosis probably associated with recreational activities in rivers in the northern part of Okinawa Main Island
    Masaji Nakamura
    Okinawa Prefectural Institute of Health and Environment, 2085 Ozato, Okinawa 901 1202, Japan
    J Vet Med Sci 68:83-5. 2006
    ..The findings indicate that recreation in rivers in this area is a significant risk factor for infection with leptospires...
  26. ncbi request reprint [A case of leptospirosis caused by Leptospira borgpetersenii serovar sejroe infected in Bali Island, Indonesia]
    Mitsuo Sakamoto
    Division of Infection Control, The Jikei University School of Medicine
    Kansenshogaku Zasshi 79:294-8. 2005
    ..In addition serovars of "imported" cases representing infection in pandemic areas differ widely from those in domestic cases. Adequate laboratory support therefore is crucial for accurate diagnosis of leptospirosis...
  27. pmc Leptospirosis in squirrels imported from United States to Japan
    Toshiyuki Masuzawa
    Laboratory of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Choshi, Japan
    Emerg Infect Dis 12:1153-5. 2006
    ..Patients worked with exotic animals in their company. Leptospira isolates from 1 patient and 5 of 10 squirrels at the company were genetically and serologically identical and were identified as Leptospira kirschneri...
  28. ncbi request reprint New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan
    Toshiyuki Masuzawa
    Department of Microbiology, and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka 422 8526, Japan
    J Med Microbiol 53:421-6. 2004
    ..valaisiana and strain Am501. These findings suggest that these isolates found in East Asia should be classified as a new genomospecies of Borrelia burgdorferi sensu lato...
  29. ncbi request reprint Case report: Borrelia valaisiana infection in a Japanese man associated with traveling to foreign countries
    Kan Saito
    Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Hongo 7 3 1, Bunkyo ku, Tokyo 113 8655, Japan
    Am J Trop Med Hyg 77:1124-7. 2007
    ..Serologic examination detected antibodies to Lyme disease Borrelia. An flaB polymerase chain reaction with the patient's plasma amplified a DNA fragment similar to that of B. valaisiana...
  30. ncbi request reprint Seroprevalence of leptospirosis and risk factor analysis in flood-prone rural areas in Lao PDR
    Leo Kawaguchi
    Department of International Health, Nagoya University School of Medicine, Nagoya, Japan
    Am J Trop Med Hyg 78:957-61. 2008
    ..This study showed that leptospirosis is endemic in Khammouane Province and that local flooding plays an important role in the transmission of the disease...