Yasushi Hiraoka

Summary

Affiliation: National Institute of Information and Communications Technology
Country: Japan

Publications

  1. pmc Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast
    Da Qiao Ding
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    J Cell Biol 174:499-508. 2006
  2. ncbi request reprint [Telomere bouquet of meiotic chromosomes]
    Yasushi Hiraoka
    Tanpakushitsu Kakusan Koso 52:145-50. 2007
  3. doi request reprint Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly
    Tokuko Haraguchi
    CREST Research Project, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, Japan
    J Cell Sci 121:2540-54. 2008
  4. pmc Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe
    Aki Hayashi
    Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Kobe 651 2492, Japan
    Mol Biol Cell 17:5173-84. 2006
  5. ncbi request reprint The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:337-52. 2006
  6. ncbi request reprint Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells
    Takeshi Shimi
    CREST of JST, Kansai Advanced Research Center, CRL, Kobe 651 2492, Japan
    J Struct Biol 147:31-41. 2004
  7. pmc Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation
    Yuji Chikashige
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Nishi ku, Kobe 651 2492, Japan
    J Cell Biol 187:413-27. 2009
  8. ncbi request reprint Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
  9. ncbi request reprint Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:319-36. 2006
  10. ncbi request reprint Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast
    Da Qiao Ding
    CREST Research Project, Kansai Advanced Research Center, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Dev Cell 6:329-41. 2004

Collaborators

Detail Information

Publications59

  1. pmc Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast
    Da Qiao Ding
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    J Cell Biol 174:499-508. 2006
    ....
  2. ncbi request reprint [Telomere bouquet of meiotic chromosomes]
    Yasushi Hiraoka
    Tanpakushitsu Kakusan Koso 52:145-50. 2007
  3. doi request reprint Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly
    Tokuko Haraguchi
    CREST Research Project, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, Japan
    J Cell Sci 121:2540-54. 2008
    ..These results suggest that the MT-mediated BAF accumulation at the core facilitates NE assembly at the end of mitosis...
  4. pmc Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe
    Aki Hayashi
    Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Kobe 651 2492, Japan
    Mol Biol Cell 17:5173-84. 2006
    ....
  5. ncbi request reprint The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:337-52. 2006
    ....
  6. ncbi request reprint Dynamic interaction between BAF and emerin revealed by FRAP, FLIP, and FRET analyses in living HeLa cells
    Takeshi Shimi
    CREST of JST, Kansai Advanced Research Center, CRL, Kobe 651 2492, Japan
    J Struct Biol 147:31-41. 2004
    ..These findings contrast with the slow mobility of both GFP-BAF and GFP-emerin during telophase, when they colocalized at the 'core' region of telophase chromosomes at early stages of nuclear assembly...
  7. pmc Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation
    Yuji Chikashige
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Nishi ku, Kobe 651 2492, Japan
    J Cell Biol 187:413-27. 2009
    ..Interestingly, the functional integrity of telomeres is maintained even when they are separated from the nuclear envelope in vegetative cells...
  8. ncbi request reprint Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes
    Yuji Chikashige
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Cell 125:59-69. 2006
    ..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB...
  9. ncbi request reprint Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes
    Hirohisa Masuda
    Cell Biology Group and CREST JST, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, 651 2492, Japan
    Genes Cells 11:319-36. 2006
    ....
  10. ncbi request reprint Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast
    Da Qiao Ding
    CREST Research Project, Kansai Advanced Research Center, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Dev Cell 6:329-41. 2004
    ....
  11. doi request reprint Meiosis-specific noncoding RNA mediates robust pairing of homologous chromosomes in meiosis
    Da Qiao Ding
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Science 336:732-6. 2012
    ..Thus, we propose that RNA transcripts retained on the chromosome play an active role in recognition of homologous chromosomes for pairing...
  12. pmc Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast
    Haruhiko Asakawa
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Mol Biol Cell 16:2325-38. 2005
    ....
  13. ncbi request reprint Meiosis induced by inactivation of Pat1 kinase proceeds with aberrant nuclear positioning of centromeres in the fission yeast Schizosaccharomyces pombe
    Yuji Chikashige
    Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Genes Cells 9:671-84. 2004
    ..As Pat1 inactivation is often useful to induce S. pombe cells synchronously into meiosis, the temporal order of chromosomal events determined here will provide landmarks for the progression of meiosis downstream the Pat1 inactivation...
  14. doi request reprint Localization of gene products using a chromosomally tagged GFP-fusion library in the fission yeast Schizosaccharomyces pombe
    Aki Hayashi
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Japan
    Genes Cells 14:217-25. 2009
    ....
  15. ncbi request reprint Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe
    Yuji Chikashige
    Cell Biology Project, Biological ICT Group, Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, and CREST Research Project, Kobe, Japan
    Cell Struct Funct 32:149-61. 2007
    ..These results suggest that additional chromosomes in aneuploids could lead to imbalances in gene expression through changes in distribution of heterochromatin as well as in gene dosage...
  16. ncbi request reprint Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 120:1967-77. 2007
    ..BAF downregulation also caused lamin A to mislocalize away from the nuclear envelope. These results indicate that BAF is required for the integrity of the nuclear lamina and normal progression of S phase in human cells...
  17. ncbi request reprint Artificial induction of autophagy around polystyrene beads in nonphagocytic cells
    Shouhei Kobayashi
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka cho, Nishi ku, Kobe, Japan
    Autophagy 6:36-45. 2010
    ..In addition, the beads were subsequently transferred to lysosomes within a couple of hours. Our findings demonstrate the cellular responses that lead to autophagy in response to pathogen invasion...
  18. ncbi request reprint Activation of the pheromone-responsive MAP kinase drives haploid cells to undergo ectopic meiosis with normal telomere clustering and sister chromatid segregation in fission yeast
    Takaharu G Yamamoto
    Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communication Technology, 588 2 Iwaoka cho, Iwaoka, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 117:3875-86. 2004
    ..In contrast, in meiosis induced by inactivation of Pat1, sister chromatids segregate precociously during the first meiotic division. Thus, these results suggest that activation of Spk1 drives meiosis in S. pombe...
  19. pmc Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast
    Ayumu Yamamoto
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    EMBO J 22:2284-96. 2003
    ..We propose that co-operation of these two mechanisms generates the high fidelity of monopolar attachment...
  20. doi request reprint From meiosis to postmeiotic events: alignment and recognition of homologous chromosomes in meiosis
    Da Qiao Ding
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe, Japan
    FEBS J 277:565-70. 2010
    ..Here, we discuss the various mechanisms used for alignment and recognition of homologous chromosomes in meiosis...
  21. doi request reprint Early entry and deformation of macropinosomes correlates with high efficiency of decaarginine-polyethylene glycol-lipid-mediated gene delivery
    Shouhei Kobayashi
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Iwaoka, Kobe, Japan
    J Gene Med 14:262-71. 2012
    ..However, the intracellular behaviour of the two different nanostructures, which leads to differences in gene delivery, remains to be elucidated...
  22. ncbi request reprint Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Iwaoka cho, Nishi ku, Kobe, Japan
    Eur J Biochem 271:1035-45. 2004
    ..These results suggest that Btf localization is regulated by apoptotic signals, and that loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy...
  23. ncbi request reprint Another way to move chromosomes
    Yuji Chikashige
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe, 651 2492, Japan
    Chromosoma 116:497-505. 2007
    ..Telomeres are then moved along the nuclear envelope using cytoplasmic microtubules. These findings illustrate a general mechanism for transmitting a cytoskeletal driving force to chromosomes across the nuclear envelope...
  24. doi request reprint Two distinct repeat sequences of Nup98 nucleoporins characterize dual nuclei in the binucleated ciliate tetrahymena
    Masaaki Iwamoto
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Kobe 651 2492, Japan
    Curr Biol 19:843-7. 2009
    ..These results suggest that Nup98s act as a barrier to misdirected localization of nucleus-specific proteins. Our findings provide the first evidence that the NPC contributes to nucleus-selective transport in ciliates...
  25. doi request reprint Nucleoporin Nup98: a gatekeeper in the eukaryotic kingdoms
    Masaaki Iwamoto
    Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Japan
    Genes Cells 15:661-9. 2010
    ..In this review, we focus on Nup98 function and discuss how this nucleoporin has evolved in eukaryotic kingdoms...
  26. doi request reprint Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena
    Masaaki Iwamoto
    Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology NICT, Kobe 651 2492, Japan
    Gene 534:249-55. 2014
    ..These results indicate that pac can be used as a selection marker in molecular studies of T. thermophila...
  27. ncbi request reprint Linear element formation and their role in meiotic sister chromatid cohesion and chromosome pairing
    Monika Molnar
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651 2492, Japan
    J Cell Sci 116:1719-31. 2003
    ..Investigation of the rec10 mutant showed that linear elements are basically dispensable for sister chromatid cohesion, but contribute to full level pairing of homologous chromosomes...
  28. ncbi request reprint [Dynamics of homologous chromosome pairing as observed in living cells of fission yeast]
    Da Qiao Ding
    Tanpakushitsu Kakusan Koso 50:131-5. 2005
  29. pmc Transcriptional Suppression by Transient Recruitment of ARIP4 to Sumoylated nuclear receptor Ad4BP/SF-1
    Hidesato Ogawa
    Division of Sex Differentiation, National Institute for Basic Biology, National Institutes of Natural Sciences, Myodaiji cho, Okazaki 444 8787, Japan
    Mol Biol Cell 20:4235-45. 2009
    ..These findings suggest that ARIP4 may be a cofactor that modulates SUMO-mediated fine-tuning of transcriptional suppression...
  30. ncbi request reprint [Chromosome dynamics during meiosis]
    Haruhiko Asakawa
    Tanpakushitsu Kakusan Koso 51:2159-64. 2006
  31. ncbi request reprint [Fluorescence imaging to visualize molecular dynamics in living cells]
    Tokuko Haraguchi
    Tanpakushitsu Kakusan Koso 51:1972-7. 2006
  32. ncbi request reprint Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase
    Tomohiro Hayakawa
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 116:3327-38. 2003
    ....
  33. ncbi request reprint Cytoplasmic dynein in fungi: insights from nuclear migration
    Ayumu Yamamoto
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    J Cell Sci 116:4501-12. 2003
    ..Recruitment of dynein to microtubules and its subsequent activation upon association with cellular structures are perhaps common to many dynein-dependent eukaryotic processes, including organelle transport...
  34. ncbi request reprint Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells
    Tetsuya Hori
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Sci 116:3347-62. 2003
    ..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic...
  35. pmc Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation
    Ayumu Yamamoto
    Department of Chemistry, Shizuoka University, Suruga ku, Shizuoka 422 8529, Japan
    J Cell Biol 182:277-88. 2008
    ..Our findings show that impacts of SAC activation are not confined to a single division at meiosis due to meiosis-specific APC/C regulation, which has probably been evolved for execution of two meiotic divisions...
  36. doi request reprint Heterochromatin integrity affects chromosome reorganization after centromere dysfunction
    Kojiro Ishii
    Division of Cell Biology, Institute of Life Science, Kurume University, Japan
    Science 321:1088-91. 2008
    ..By affecting the modes of chromosomal reorganization, the genomic distribution of heterochromatin may influence the fate of karyotype evolution...
  37. ncbi request reprint Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe
    Eveline Doll
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012, Bern, Switzerland
    Curr Genet 48:323-33. 2005
    ..The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis...
  38. ncbi request reprint Functional expression of human mitochondrial CYP11B2 in fission yeast and identification of a new internal electron transfer protein, etp1
    Matthias Bureik
    Universitat des Saarlandes, FR 8 8 Biochemie, Postfach 151150, D 66041 Saarbrucken, Germany
    Biochemistry 41:2311-21. 2002
    ..Therefore, we suggest to name this protein etp1 (electron-transfer protein 1)...
  39. ncbi request reprint Spectral imaging fluorescence microscopy
    Tokuko Haraguchi
    CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
    Genes Cells 7:881-7. 2002
    ....
  40. ncbi request reprint [Genome sequence of Schizosaccharomyces pombe]
    Yuji Chikashige
    Tanpakushitsu Kakusan Koso 47:1215-20. 2002
  41. ncbi request reprint CENP-I is essential for centromere function in vertebrate cells
    Ai Nishihashi
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411 8540, Shizuoka, Japan
    Dev Cell 2:463-76. 2002
    ..Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere...
  42. pmc The 14-kDa dynein light chain-family protein Dlc1 is required for regular oscillatory nuclear movement and efficient recombination during meiotic prophase in fission yeast
    Futaba Miki
    Kazusa DNA Research Institute, Kisarazu 292 0812, Japan
    Mol Biol Cell 13:930-46. 2002
    ..S. pombe contains a homolog of the 8-kDa dynein light chain, Dlc2. This class of dynein light chain, however, is not essential in either the vegetative or sexual phases...
  43. ncbi request reprint Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe
    Mizuki Shimanuki
    Initial Research Project IRP, Okinawa Institute of Science and Technology Promotion Corporation OIST, Uruma 904 2234, Okinawa, Japan
    Genes Cells 12:677-92. 2007
    ....
  44. pmc The constitutive centromere component CENP-50 is required for recovery from spindle damage
    Yukinori Minoshima
    National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:10315-28. 2005
    ..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage...
  45. pmc Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome
    Eishi Funakoshi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka 573 0101, Japan
    BMC Cell Biol 4:12. 2003
    ..This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But, the functions of the MNB/DYRK1A gene in cellular processes are unknown...
  46. pmc Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis
    Fabian Oceguera-Yanez
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    J Cell Biol 168:221-32. 2005
    ..These results suggest that Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis...
  47. pmc A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2+, a homolog of the human gene predisposing to tuberous sclerosis complex
    Yukiko Nakase
    Radiation Biology Center, Kyoto University, Japan
    Genetics 173:569-78. 2006
    ..On the basis of these results, we discuss TSC pathology and possible improvement in chemotherapy for TSC...
  48. ncbi request reprint ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
    Akihisa Matsuyama
    Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Nat Biotechnol 24:841-7. 2006
    ..Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1...
  49. ncbi request reprint Selective elimination of messenger RNA prevents an incidence of untimely meiosis
    Yuriko Harigaya
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Nature 442:45-50. 2006
    ..We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts...
  50. ncbi request reprint Functional domain analysis of human HP1 isoforms in Drosophila
    Masaki Kato
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Cell Struct Funct 32:57-67. 2007
    ..These results suggest human HP1 subtypes use different domains to suppress gene expression in Drosophila cells...
  51. pmc Cellular stresses induce the nuclear accumulation of importin alpha and cause a conventional nuclear import block
    Yoichi Miyamoto
    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    J Cell Biol 165:617-23. 2004
    ..These findings suggest that the stress-induced nuclear accumulation of importin alpha can be involved in a common physiological response to various stress conditions...
  52. pmc Peroxisomes are formed from complex membrane structures in PEX6-deficient CHO cells upon genetic complementation
    Noriyo Hashiguchi
    Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678 1297, Japan
    Mol Biol Cell 13:711-22. 2002
    ..Our results indicate that 1) Peroxisomal ghosts are complex membrane structures; and 2) The complex membrane structures become import competent and are converted into peroxisomes upon complementation with PEX6...
  53. ncbi request reprint Assembly of additional heterochromatin distinct from centromere-kinetochore chromatin is required for de novo formation of human artificial chromosome
    Hiroshi Nakashima
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Cell Sci 118:5885-98. 2005
    ....
  54. ncbi request reprint Heat-shock induced nuclear retention and recycling inhibition of importin alpha
    Maiko Furuta
    Cellular Dynamics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Genes Cells 9:429-41. 2004
    ..This suggests a new regulatory mechanism for the adaptation of cells to environmental changes, such as heat-shock...
  55. ncbi request reprint Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins
    Ai Kametaka
    Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Japan
    Genes Cells 7:1231-42. 2002
    ..The possible human homologue of chmadrin, Ki-67 antigen (pKi-67), also has a stretch of LR pairs, but with no obvious overall similarity, at its C-terminus...
  56. ncbi request reprint Multispectral imaging fluorescence microscopy for living cells
    Yasushi Hiraoka
    Kansai Advanced Research Center, Communications Research Laboratory, Kobe, Japan
    Cell Struct Funct 27:367-74. 2002
    ..We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering...
  57. pmc Cadherin activity is required for activity-induced spine remodeling
    Ko Okamura
    Department of Pharmacology, Osaka University Medical School, Suita, Japan
    J Cell Biol 167:961-72. 2004
    ..Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone...
  58. ncbi request reprint Mutations in dynein link motor neuron degeneration to defects in retrograde transport
    Majid Hafezparast
    Department of Neurodegenerative Disease, Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK
    Science 300:808-12. 2003
    ..These mutations exclusively perturb neuron-specific functions of dynein...
  59. ncbi request reprint Nuclear structure-associated TIF2 recruits glucocorticoid receptor and its target DNA
    Hidesato Ogawa
    Department of Biochemistry, Institute for Virus Research, Kyoto University, Japan
    Biochem Biophys Res Commun 320:218-25. 2004
    ..The TIF2 foci could recruit GR carrying a microinjected GR responsive element. We propose that TIF2 provides a nuclear compartment that allows the assembly of multi-protein complexes required for GR-mediated gene activation...