Shigeaki Ishizaka

Summary

Affiliation: Nara Medical University
Country: Japan

Publications

  1. ncbi Development of hepatocytes from ES cells after transfection with the HNF-3beta gene
    Shigeaki Ishizaka
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan
    FASEB J 16:1444-6. 2002
  2. pmc Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells
    Ko Saito
    Department of Gastroenterology and Hepatology, Nara Medical University, Nara, Japan
    World J Gastroenterol 12:6818-27. 2006
  3. pmc Embryonic stem cells reduce liver fibrosis in CCl4-treated mice
    Kei Moriya
    Third Department of Internal Medicine, Nara Medical University, Kashihara, Nara, Japan
    Int J Exp Pathol 89:401-9. 2008
  4. doi A familial case of visceral toxocariasis due to consumption of raw bovine liver
    Masahide Yoshikawa
    Department of Parasitology, Nara Medical University, Japan
    Parasitol Int 57:525-9. 2008
  5. pmc Differentiation of embryonic stem cells into insulin-producing cells promoted by Nkx2.2 gene transfer
    Akira Shiroi
    Division of Developmental Biology, Department of Parasitology, Nara Medical University, Kashihara, Nara 634 8521, Japan
    World J Gastroenterol 11:4161-6. 2005
  6. pmc Embryonic stem cells develop into hepatocytes after intrasplenic transplantation in CCl4-treated mice
    Kei Moriya
    Division of Hepatology, Third Department of Internal Medicine, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    World J Gastroenterol 13:866-73. 2007
  7. doi CoCl(2) inhibits neural differentiation of retinoic acid-treated embryoid bodies
    Jun ichi Nonaka
    Department of Neurosurgery, Nara Medical University, 840 Shijo Cho, Kashihara, Nara, Japan
    J Biosci Bioeng 106:141-7. 2008
  8. doi Treatment of Parkinson's disease model mice with allogeneic embryonic stem cells: necessity of immunosuppressive treatment for sustained improvement
    Hayato Toriumi
    Department of Neurosurgery, Nara Medical University, 840 Shijo Cho, Kashihara shi, Nara 634 8521, Japan
    Neurol Res 31:220-7. 2009
  9. ncbi In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green
    Takatsugu Yamada
    Division of Developmental Biology, Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    Stem Cells 20:146-54. 2002
  10. doi Modulated differentiation of embryonic stem cells into hepatocyte-like cells by coculture with hepatic stellate cells
    Mariko Nishiofuku
    Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara 634 8521, Japan
    J Biosci Bioeng 111:71-7. 2011

Collaborators

Detail Information

Publications38

  1. ncbi Development of hepatocytes from ES cells after transfection with the HNF-3beta gene
    Shigeaki Ishizaka
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan
    FASEB J 16:1444-6. 2002
    ....
  2. pmc Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells
    Ko Saito
    Department of Gastroenterology and Hepatology, Nara Medical University, Nara, Japan
    World J Gastroenterol 12:6818-27. 2006
    ..To explore whether a co-culture of cynomolgus monkey embryonic stem (cES) cells with embryonic liver cells could promote their differentiation into hepatocytes...
  3. pmc Embryonic stem cells reduce liver fibrosis in CCl4-treated mice
    Kei Moriya
    Third Department of Internal Medicine, Nara Medical University, Kashihara, Nara, Japan
    Int J Exp Pathol 89:401-9. 2008
    ....
  4. doi A familial case of visceral toxocariasis due to consumption of raw bovine liver
    Masahide Yoshikawa
    Department of Parasitology, Nara Medical University, Japan
    Parasitol Int 57:525-9. 2008
    ..The patients responded well to treatment with albendazole. Ingestion of raw liver from paratenic animals is considered to be a common transmission route of human toxocariasis, especially in adults...
  5. pmc Differentiation of embryonic stem cells into insulin-producing cells promoted by Nkx2.2 gene transfer
    Akira Shiroi
    Division of Developmental Biology, Department of Parasitology, Nara Medical University, Kashihara, Nara 634 8521, Japan
    World J Gastroenterol 11:4161-6. 2005
    ..To investigate the ability of a genetically altered embryonic stem (ES) cell line to generate insulin-producing cells in vitro following transfer of the Nkx2.2 gene...
  6. pmc Embryonic stem cells develop into hepatocytes after intrasplenic transplantation in CCl4-treated mice
    Kei Moriya
    Division of Hepatology, Third Department of Internal Medicine, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    World J Gastroenterol 13:866-73. 2007
    ..To transplant undifferentiated embryonic stem (ES) cells into the spleens of carbon tetrachloride (CCl4)-treated mice to determine their ability to differentiate into hepatocytes in the liver...
  7. doi CoCl(2) inhibits neural differentiation of retinoic acid-treated embryoid bodies
    Jun ichi Nonaka
    Department of Neurosurgery, Nara Medical University, 840 Shijo Cho, Kashihara, Nara, Japan
    J Biosci Bioeng 106:141-7. 2008
    ..These results suggest that CoCl(2) inhibits neural differentiation of RA-treated EB cells and promotes the proliferation of nestin-immunopositive cells, i.e., embryonic stem (ES)-derived neural stem-like cells...
  8. doi Treatment of Parkinson's disease model mice with allogeneic embryonic stem cells: necessity of immunosuppressive treatment for sustained improvement
    Hayato Toriumi
    Department of Neurosurgery, Nara Medical University, 840 Shijo Cho, Kashihara shi, Nara 634 8521, Japan
    Neurol Res 31:220-7. 2009
    ..The purpose of the present study was to examine the efficacy of transplantation of mouse embryonic stem (ES) into Parkinson's disease (PD) model mice as well as the necessity of immunosuppression in allogeneic donor-host combinations...
  9. ncbi In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green
    Takatsugu Yamada
    Division of Developmental Biology, Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    Stem Cells 20:146-54. 2002
    ..ICG is clinically used as a test substance to evaluate liver function because it is eliminated exclusively by hepatocytes. The aim of the present study was to investigate the hepatic characteristics of ICG-stained cells...
  10. doi Modulated differentiation of embryonic stem cells into hepatocyte-like cells by coculture with hepatic stellate cells
    Mariko Nishiofuku
    Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara 634 8521, Japan
    J Biosci Bioeng 111:71-7. 2011
    ..Thus, HSCs have contrasting effects on EBs undergoing differentiation and undifferentiated ES cells, i.e., positive and negative modulation, respectively...
  11. ncbi Transplantation of embryonic stem cell-derived neural stem cells for spinal cord injury in adult mice
    Hajime Kimura
    Department of Neurosurgery, Nara Medical University, Nara, Japan
    Neurol Res 27:812-9. 2005
    ..To investigate the efficacy of embryonic stem cell-derived neural stem cells (NSCs) for spinal cord injury (SCI) in mice and whether a combination treatment with thyroid hormone provides a more effective ES cell-based therapy...
  12. ncbi Cynomolgus monkey embryonic stem cell lines express green fluorescent protein
    Shigehiko Ueda
    Department of Gastroenterology and Hepatology, Nara Medical University, Kashihara, Nara 634 8521, Japan
    J Biosci Bioeng 102:14-20. 2006
    ..GFP expression was also observed in the differentiated cells. These GFP-expressing cES cell lines are considered useful for basic research, including cell transplantation...
  13. ncbi Cotransplantation of mouse embryonic stem cells and bone marrow stromal cells following spinal cord injury suppresses tumor development
    Ryosuke Matsuda
    Department of Neurosurgery, Nara Medical University, Nara 634 8521, Japan
    Cell Transplant 18:39-54. 2009
    ..Cotransplantation of BMSCs with ES cell-derived graft cells may be useful for preventing the development of ES cell-derived tumors...
  14. ncbi Intraventricular transplantation of embryonic stem cell-derived neural stem cells in intracerebral hemorrhage rats
    Masahiro Nonaka
    Department of Neurosurgery, Nara Medical University, Kashihara, Japan
    Neurol Res 26:265-72. 2004
    ....
  15. ncbi Use of differentiating embryonic stem cells in the Parkinsonian mouse model
    Fumihiko Nishimura
    Division of Development Biology, Department of Parasitology, Nara Medical University, Japan
    Methods Mol Biol 329:485-93. 2006
    ....
  16. ncbi Wnt-10b, uniquely among Wnts, promotes epithelial differentiation and shaft growth
    Yukiteru Ouji
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    Biochem Biophys Res Commun 367:299-304. 2008
    ..Our results suggest that Wnt-10b is unique and plays an important role in differentiation of epithelial cells in the hair follicle...
  17. ncbi Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells
    Yukiteru Ouji
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan
    Biochem Biophys Res Commun 342:1063-9. 2006
    ..These results suggest that Wnt-10b promotes the differentiation of MPSEC and may play an important role in hair follicle development by promoting differentiation of epithelial cells...
  18. ncbi Effects of Wnt-10b on hair shaft growth in hair follicle cultures
    Yukiteru Ouji
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara 634 8521, Japan
    Biochem Biophys Res Commun 359:516-22. 2007
    ....
  19. doi Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart
    Yukiteru Ouji
    Program in Tissue Engineering, Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    J Biosci Bioeng 110:487-90. 2010
    ..This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size...
  20. doi Effects of Wnt-10b on proliferation and differentiation of adult murine skin-derived CD34 and CD49f double-positive cells
    Yukiteru Ouji
    Program in Tissue Engineering, Department of Parasitology, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    J Biosci Bioeng 110:217-22. 2010
    ..These results suggest that Wnt-10b promotes differentiation of epithelial stem/progenitor cells in the skin...
  21. ncbi Promotion of hair follicle development and trichogenesis by Wnt-10b in cultured embryonic skin and in reconstituted skin
    Yukiteru Ouji
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan
    Biochem Biophys Res Commun 345:581-7. 2006
    ..Without the co-transplantation of Wnt-COS, no hair growth was observed. Our results suggest an important role of Wnt-10b in the initiation of hair follicle development and following trichogenesis...
  22. ncbi Derivation and characterization of hepatocytes from embryonic stem cells in vitro
    Shigeaki Ishizaka
    Program in Tissue Engineering, Department of Parasitology, Nara Medical University, Japan
    Methods Mol Biol 330:387-99. 2006
    ..We have explored a three-dimensional culture system in which hepatocytes differentiated from mouse ES cells by transfection with the hepatocyte nuclear factor-3beta possess high metabolic functions that can be maintained long term...
  23. ncbi DACS, novel matrix structure composed of chondroitin sulfate proteoglycan in the brain
    Noriko Hayashi
    Department of Parasitology, Faculty of Medicine, Nara Medical University, Kashihara, Nara 634 8521, Japan
    Biochem Biophys Res Commun 364:410-5. 2007
    ..This is the first report demonstrating the presence of an ECM structure DACS composed of CSPGs around a group of cortical neurons in the adult cerebral cortex...
  24. ncbi Potential use of embryonic stem cells for the treatment of mouse parkinsonian models: improved behavior by transplantation of in vitro differentiated dopaminergic neurons from embryonic stem cells
    Fumihiko Nishimura
    Department of Parasitology, Nara Medical University, Japan
    Stem Cells 21:171-80. 2003
    ....
  25. ncbi Expression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats
    Hiroshi Yokota
    Department of Neurosurgery, Nara Medical University, 840 Shijo Cho, Kashihara, Nara 634 8521, Japan
    Neurosci Lett 377:170-5. 2005
    ..Our findings suggest differential regulation of CNTF and CNTFR-alpha, and the possibility of a therapeutic strategy using CNTF administration for ICH...
  26. doi Localization of chondroitin sulfate proteoglycan versican in adult brain with special reference to large projection neurons
    Noriko Horii-Hayashi
    Department of Parasitology, Faculty of Medicine, Nara Medical University, Kashihara, Nara, 634 8521, Japan
    Cell Tissue Res 334:163-77. 2008
    ..These treatments had no effect on the labeling pattern of versican, suggesting that other versican-interactive molecules are involved in the binding of versican to neurons...
  27. ncbi Surgical removal of a live worm by stereotactic targeting in cerebral sparganosis. Case report
    Misato Nobayashi
    Department of Neurosurgery, Nara Medical University, Nara
    Neurol Med Chir (Tokyo) 46:164-7. 2006
    ..The infection route in the present case was probably through eating raw chicken meat. Cerebral sparganosis is extremely rare but should be considered in the differential diagnosis of metastatic brain tumors, especially in endemic areas...
  28. ncbi Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina
    Yuko Sugie
    Department of Ophthalmology, Nara Medical University, Nara, Kashihara Shi 630 8522, Japan
    Biochem Biophys Res Commun 332:241-7. 2005
    ..These results indicate that co-culture of ES cells with chick embryonic retina tissue is a useful and efficient method for the induction of photoreceptor-like cells...
  29. ncbi Visceral toxocariasis from regular consumption of raw cow liver
    Masahide Yoshikawa
    Department of Parasitology, Nara Medical University, Kashihara, Japan
    Intern Med 47:1289-90. 2008
  30. doi Diagnostic problems in a patient with amicrofilaremic Loa loa
    Masahide Yoshikawa
    Department of Parasitology, Nara Medical University School of Medicine, Kashihara, Japan
    J Travel Med 15:53-7. 2008
    ..It is important to note that most infected travelers whose home countries are in nonendemic regions are amicrofilaremic...
  31. doi Dermal papilla cells serially cultured with Wnt-10b sustain their hair follicle induction activity after transplantation into nude mice
    Yukiteru Ouji
    Department of Pathogen, Infection, and Immunity, Nara Medical University, Kashihara, Nara, Japan
    Cell Transplant 21:2313-24. 2012
    ..These results suggest that Wnt-10b plays a pivotal role in proliferation and maintenance of DP cells in vitro...
  32. ncbi Identification of insulin-producing cells derived from embryonic stem cells by zinc-chelating dithizone
    Akira Shiroi
    Division of Developmental Biology, Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan
    Stem Cells 20:284-92. 2002
    ..The aim of the present study was to investigate the characteristics of DTZ-stained cellular clusters originating from ES cells...
  33. doi Environmental stimulation changes tissue-type plasminogen activator activity in the adult mouse hippocampus
    Noriko Horii-Hayashi
    Department of Anatomy and Cell Biology, Faculty of Medicine, Nara Medical University, Kashihara, Nara, Japan
    Neurochem Int 58:1-4. 2011
    ..These results suggest that tPA activity is regulated reversibly in an experience-dependent manner in the adult hippocampus...
  34. ncbi Wnt-10b promotes differentiation of skin epithelial cells in vitro
    Yukiteru Ouji
    Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan
    Biochem Biophys Res Commun 342:28-35. 2006
    ..These results suggest that Wnt-10b promotes the differentiation of MPSEC...
  35. ncbi In vitro functional gut-like organ formation from mouse embryonic stem cells
    Takatsugu Yamada
    Division of Developmental Biology, Department of Parasitology, Nara Medical University, Nara, Japan
    Stem Cells 20:41-9. 2002
    ..The aim of the present study was to investigate the electrophysiological and morphological properties of ES cell-derived contracting clusters...
  36. doi Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro
    Yukiteru Ouji
    1 Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Japan 2 Program in Tissue Engineering, Department of Parasitology, Nara Medical University, Kashihara, Japan
    J Invest Dermatol 135:1598-608. 2015
    ..Our results suggest the possibility of partial long-term maintenance of EpSCs in vitro by Wnt-3a. ..
  37. ncbi Cystic echinococcosis in immigrant from Peru: first case treated with percutaneous treatment in Japan
    Hiroshi Sakaguchi
    Department of Radiology, Nara Medical University, Kashihara 634 8521, Japan
    Parasitol Int 56:207-10. 2007
    ..We consider that percutaneous treatment is a safe therapeutic modality for hydatid cysts. This is the first case report of CE treated percutaneously in Japan...
  38. ncbi Expressions of hepatobiliary organic anion transporters and bilirubin-conjugating enzyme in differentiating embryonic stem cells
    Yuji Tanaka
    Third Department of Internal Medicine, Mie University School of Medicine, 2 174 Edobashi, Tsu city 514 8507, Japan
    Biochem Biophys Res Commun 309:324-30. 2003
    ....