Keizo Suzuki

Summary

Affiliation: Kyoto Institute of Technology
Country: Japan

Publications

  1. doi request reprint Early Days of Pressure Denaturation Studies of Proteins
    Keizo Suzuki
    Department of Chemistry, College of Science and Engineering, Ritsumeikan University, 1 1 1, Nojihigashi, Kusatsu, Shiga, 525 8577, Japan
    Subcell Biochem 72:3-17. 2015
  2. doi request reprint Enhancement of glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase of Bacillus subtilis
    Hideyuki Suzuki
    Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Biotechnol J 5:829-37. 2010
  3. pmc Mechanism for regulation of the putrescine utilization pathway by the transcription factor PuuR in Escherichia coli K-12
    Naoki Nemoto
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido cho, Matsugasaki, Sakyo ku, Kyoto, Japan
    J Bacteriol 194:3437-47. 2012
  4. doi request reprint gamma-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12
    Shin Kurihara
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    J Biol Chem 283:19981-90. 2008
  5. pmc The putrescine Importer PuuP of Escherichia coli K-12
    Shin Kurihara
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido cho, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Bacteriol 191:2776-82. 2009
  6. pmc A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12
    Shin Kurihara
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido cho Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Bacteriol 192:4582-91. 2010
  7. pmc Identification of the LIV-I/LS system as the third phenylalanine transporter in Escherichia coli K-12
    Takashi Koyanagi
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    J Bacteriol 186:343-50. 2004
  8. ncbi request reprint Thermal denaturation and renaturation of γ-glutamyltranspeptidase of Escherichia coli
    Thao Van Ho
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
    Biosci Biotechnol Biochem 77:409-12. 2013
  9. doi request reprint Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119
    Uschara Thumarat
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Appl Microbiol Biotechnol 95:419-30. 2012
  10. doi request reprint Enzymatic synthesis of γ-glutamylglutamine, a stable glutamine analogue, by γ-glutamyltranspeptidase from Escherichia coli K-12
    Ashraf Sabry Abdel Fatah El Sayed
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Biotechnol Lett 32:1877-81. 2010

Collaborators

Detail Information

Publications12

  1. doi request reprint Early Days of Pressure Denaturation Studies of Proteins
    Keizo Suzuki
    Department of Chemistry, College of Science and Engineering, Ritsumeikan University, 1 1 1, Nojihigashi, Kusatsu, Shiga, 525 8577, Japan
    Subcell Biochem 72:3-17. 2015
    ..Here I review how I started experiments and analysis on pressure denaturation of proteins in early days of 1950s and 1960s in my laboratory and others. ..
  2. doi request reprint Enhancement of glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase of Bacillus subtilis
    Hideyuki Suzuki
    Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan
    Biotechnol J 5:829-37. 2010
    ..508 s(-1) for a D445G mutant enzyme. Consequently, the catalytic efficiency and the turnover rate were improved up to about 1000-fold and 10-fold, compared with the wildtype gamma-glutamyltranspeptidase of B. subtilis...
  3. pmc Mechanism for regulation of the putrescine utilization pathway by the transcription factor PuuR in Escherichia coli K-12
    Naoki Nemoto
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido cho, Matsugasaki, Sakyo ku, Kyoto, Japan
    J Bacteriol 194:3437-47. 2012
    ..E. coli and related enterobacteria may have gained the Puu pathway as an adaptation for survival in the mammalian intestine, an environment in which polyamines exist at relatively high concentrations...
  4. doi request reprint gamma-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12
    Shin Kurihara
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    J Biol Chem 283:19981-90. 2008
    ..It is suggested that the intracellular concentration of putrescine is optimized by PuuA transcriptionally and posttranslationally and that excess putrescine is converted to a nutrient source by the Puu pathway...
  5. pmc The putrescine Importer PuuP of Escherichia coli K-12
    Shin Kurihara
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido cho, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Bacteriol 191:2776-82. 2009
    ..When E. coli was grown in M9-tryptone medium, PuuP was expressed mainly in the exponential growth phase, and PotFGHI was expressed independently of the growth phase...
  6. pmc A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12
    Shin Kurihara
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido cho Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    J Bacteriol 192:4582-91. 2010
    ..The results demonstrate that the PuuE-YneI pathway was a putrescine-inducible GABA degradation pathway for utilizing putrescine as a nutrient source...
  7. pmc Identification of the LIV-I/LS system as the third phenylalanine transporter in Escherichia coli K-12
    Takashi Koyanagi
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    J Bacteriol 186:343-50. 2004
    ..The physiological importance of the LIV-I/LS system for phenylalanine accumulation was revealed in the growth of phenylalanine-auxotrophic E. coli strains under various conditions...
  8. ncbi request reprint Thermal denaturation and renaturation of γ-glutamyltranspeptidase of Escherichia coli
    Thao Van Ho
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
    Biosci Biotechnol Biochem 77:409-12. 2013
    ..coli γ-glutamyltranspeptidase was separated into protomers by heat-treatment, but was renatured by incubation at 4 °C...
  9. doi request reprint Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119
    Uschara Thumarat
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Appl Microbiol Biotechnol 95:419-30. 2012
    ..The mutant was further activated by 2.6-fold (299 u/mg) with 300 mM Ca(2+) and was stable up to 60 °C with 150 mM Ca(2+). Another identical gene was located in tandem in the upstream of est119...
  10. doi request reprint Enzymatic synthesis of γ-glutamylglutamine, a stable glutamine analogue, by γ-glutamyltranspeptidase from Escherichia coli K-12
    Ashraf Sabry Abdel Fatah El Sayed
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Biotechnol Lett 32:1877-81. 2010
    ..The maximum yield of γ-glutamylglutamine (110 mM) was obtained using 250 mM L: -glutamine and 1.1 U γ-glutamyltranspeptidase/ml at pH 10.5 and at 37°C for 7 h; the conversion of glutamine to γ-glutamylglutamine was 88%...
  11. pmc Functional analysis of the Erwinia herbicola tutB gene and its product
    Takane Katayama
    Applied Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    J Bacteriol 184:3135-41. 2002
    ..Tryptophan acted as a competitive inhibitor of tyrosine transport. Unlike the tryptophanase operon, the tpl and tutB genes do not constitute an operon...
  12. ncbi request reprint Gamma-glutamyl-gamma-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12
    Shin Kurihara
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    FEMS Microbiol Lett 256:318-23. 2006
    ....