Kazunori Shimizu

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint Evaluation systems of generated forces of skeletal muscle cell-based bio-actuators
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi, Sakyo, Kyoto 606 8501, Japan
    J Biosci Bioeng 115:115-21. 2013
  2. pmc In vivo site-specific transfection of naked plasmid DNA and siRNAs in mice by using a tissue suction device
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 7:e41319. 2012
  3. doi request reprint Implantable pneumatically actuated microsystem for renal pressure-mediated transfection in mice
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Control Release 159:85-91. 2012
  4. doi request reprint Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice
    Kosuke Kusamori
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 173:119-24. 2014
  5. doi request reprint Increased Insulin Secretion from Insulin-Secreting Cells by Construction of Mixed Multicellular Spheroids
    Kosuke Kusamori
    Department of Biopharmaceutics and Drug Metabolism Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    Pharm Res 33:247-56. 2016
  6. doi request reprint Optimization of renal transfection using a renal suction-mediated transfection method in mice
    Yota Taniguchi
    a Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Drug Target 24:450-6. 2016
  7. ncbi request reprint Liver suction-mediated transfection in mice using a pressure-controlled computer system
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University
    Biol Pharm Bull 37:569-75. 2014
  8. doi request reprint Microfluidic devices for construction of contractile skeletal muscle microtissues
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan Ritusmeikan Global Innovation Research Organization R GIRO, Ritsumeikan University, Noji higashi, Kusatsu, Shiga 525 8577, Japan Electronic address
    J Biosci Bioeng 119:212-6. 2015
  9. doi request reprint Development of a suction device for stabilizing in vivo real-time imaging of murine tissues
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan
    J Biosci Bioeng 112:508-10. 2011
  10. doi request reprint Poly(N-isopropylacrylamide)-coated microwell arrays for construction and recovery of multicellular spheroids
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan
    J Biosci Bioeng 115:695-9. 2013

Collaborators

  • Mitsuru Hashida
  • Makiya Nishikawa
  • Yuriko Higuchi
  • Kosuke Kusamori
  • Satoshi Konishi
  • Yota Taniguchi
  • Narumi Mizuno
  • Tomoko Nishikawa
  • Yuki Takahashi
  • Yoshinobu Takakura
  • Akira Masuzawa
  • Fumiyoshi Yamashita
  • Yuki Fuchigami
  • Shigeru Kawakami
  • Yuya Mizukami
  • Yutaro Tanaka
  • Natsuko Oyama

Detail Information

Publications10

  1. doi request reprint Evaluation systems of generated forces of skeletal muscle cell-based bio-actuators
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi, Sakyo, Kyoto 606 8501, Japan
    J Biosci Bioeng 115:115-21. 2013
    ..Such systems can be used to study the physiology of the skeletal muscle, screen drug candidates for skeletal muscle-related disorders, and improve the function of tissue-engineered skeletal muscle...
  2. pmc In vivo site-specific transfection of naked plasmid DNA and siRNAs in mice by using a tissue suction device
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 7:e41319. 2012
    ..These results indicate that the in vivo transfection method developed in this study will be a viable approach for biological research and therapies using nucleic acids...
  3. doi request reprint Implantable pneumatically actuated microsystem for renal pressure-mediated transfection in mice
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    J Control Release 159:85-91. 2012
    ..This is the first report of an implantable MEMS-based microsystem that demonstrates in vivo transfection to a kidney using naked plasmid DNA...
  4. doi request reprint Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice
    Kosuke Kusamori
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshidashimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    J Control Release 173:119-24. 2014
    ..These results indicate that insulin-secreting NIT-1 spheroids prepared using PNIPAAm-coated PDMS-based microwells are more effective for the treatment of type 1 diabetes than dissociated cells in suspension. ..
  5. doi request reprint Increased Insulin Secretion from Insulin-Secreting Cells by Construction of Mixed Multicellular Spheroids
    Kosuke Kusamori
    Department of Biopharmaceutics and Drug Metabolism Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    Pharm Res 33:247-56. 2016
    ..We previously have shown that multicellular spheroids containing insulin-secreting cells are an effective therapy for diabetic mice. Here we attempted to increase insulin secretion by incorporating other cell types into spheroids...
  6. doi request reprint Optimization of renal transfection using a renal suction-mediated transfection method in mice
    Yota Taniguchi
    a Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    J Drug Target 24:450-6. 2016
    ..We previously developed a suction-mediated transfection method in mice...
  7. ncbi request reprint Liver suction-mediated transfection in mice using a pressure-controlled computer system
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University
    Biol Pharm Bull 37:569-75. 2014
    ..Thus, the developed system is useful for performing the tissue suction method with high accuracy and safety. ..
  8. doi request reprint Microfluidic devices for construction of contractile skeletal muscle microtissues
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan Ritusmeikan Global Innovation Research Organization R GIRO, Ritsumeikan University, Noji higashi, Kusatsu, Shiga 525 8577, Japan Electronic address
    J Biosci Bioeng 119:212-6. 2015
    ..Thus, the microfluidic device for culturing 3D skeletal muscle microtissues presented in this study has a potential to be used for drug discovery and toxicological tests. ..
  9. doi request reprint Development of a suction device for stabilizing in vivo real-time imaging of murine tissues
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan
    J Biosci Bioeng 112:508-10. 2011
    ..In this study, we propose a suction device for stabilizing live imaging, which was used to demonstrate fluorescence detection of circulating blood cells in vessels of mouse livers...
  10. doi request reprint Poly(N-isopropylacrylamide)-coated microwell arrays for construction and recovery of multicellular spheroids
    Kazunori Shimizu
    Institute for Innovative NanoBio Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan
    J Biosci Bioeng 115:695-9. 2013
    ..Functional assessment suggests that the PNIPAAm microwell arrays are not toxic to the formed spheroids. The PNIPAAm microwell array developed in the present study will be useful for constructing and harvesting spheroids...