Tomoji Mashimo

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. pmc Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    PLoS ONE 5:e8870. 2010
  2. pmc Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Sci Rep 3:1253. 2013
  3. doi Generation and characterization of severe combined immunodeficiency rats
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Rep 2:685-94. 2012
  4. pmc The Israeli strain IS-98-ST1 of West Nile virus as viral model for West Nile encephalitis in the Old World
    Marianne Lucas
    Unité des Interactions Moléculaires Flavivirus Hôtes, Institut Pasteur, Paris, France
    Virol J 1:9. 2004
  5. pmc A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    BMC Genet 7:19. 2006
  6. ncbi Rat resources in biomedical research
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Curr Pharm Biotechnol 10:214-20. 2009
  7. doi A missense mutation of the gene encoding voltage-dependent sodium channel (Nav1.1) confers susceptibility to febrile seizures in rats
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Neurosci 30:5744-53. 2010
  8. ncbi Evaluation of LEXF/FXLE rat recombinant inbred strains for genetic dissection of complex traits
    Birger Voigt
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto
    Physiol Genomics 32:335-42. 2008
  9. doi Genetic and phenotypic characterization of a Japanese wild-derived DOB/Oda rat strain
    Takashi Kuramoto
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Mamm Genome 24:303-8. 2013
  10. doi Kcna1-mutant rats dominantly display myokymia, neuromyotonia and spontaneous epileptic seizures
    Saeko Ishida
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Brain Res 1435:154-66. 2012

Collaborators

Detail Information

Publications26

  1. pmc Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    PLoS ONE 5:e8870. 2010
    ..Recently, zinc-finger nucleases (ZFNs) were successfully used to create genome-specific double-stranded breaks and thereby induce targeted gene mutations in a wide variety of organisms including plants, drosophila, zebrafish, etc...
  2. pmc Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Sci Rep 3:1253. 2013
    ..These findings demonstrate TALENs with Exo1 is an easy and efficient method of generating gene knockouts using zygotes, which increases the range of gene targeting technologies available to various species...
  3. doi Generation and characterization of severe combined immunodeficiency rats
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Rep 2:685-94. 2012
    ....
  4. pmc The Israeli strain IS-98-ST1 of West Nile virus as viral model for West Nile encephalitis in the Old World
    Marianne Lucas
    Unité des Interactions Moléculaires Flavivirus Hôtes, Institut Pasteur, Paris, France
    Virol J 1:9. 2004
    ..These results demonstrate that Israeli strain IS-98-ST1 provides a suitable viral model for WNV-induced disease associated with recent WNV outbreaks in the Old World...
  5. pmc A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    BMC Genet 7:19. 2006
    ..Here, we report on a set of 357 simple sequence length polymorphism (SSLP) markers and 122 rat strains, which were genotyped by the marker set...
  6. ncbi Rat resources in biomedical research
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Curr Pharm Biotechnol 10:214-20. 2009
    ..Globally acting rat resource centers are prerequisites for successful and sustainable research in the biomedical field where the rats are used as model species...
  7. doi A missense mutation of the gene encoding voltage-dependent sodium channel (Nav1.1) confers susceptibility to febrile seizures in rats
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Neurosci 30:5744-53. 2010
    ..These findings suggest that a missense mutation (N1417H) of the Na(v)1.1 channel confers susceptibility to FS and the impaired biophysical properties of inhibitory GABAergic neurons underlie one of the mechanisms of FS...
  8. ncbi Evaluation of LEXF/FXLE rat recombinant inbred strains for genetic dissection of complex traits
    Birger Voigt
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto
    Physiol Genomics 32:335-42. 2008
    ..The LEXF/FXLE RI strains have been deposited at the National Bio Resource Project for the Rat in Japan and are maintained under specific pathogen-free conditions. They are available at http://www.anim.med.kyoto-u.ac.jp/nbr...
  9. doi Genetic and phenotypic characterization of a Japanese wild-derived DOB/Oda rat strain
    Takashi Kuramoto
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Mamm Genome 24:303-8. 2013
    ..Therefore, the new DOB/Oda strain will provide an important genome resource that will be useful for designing genetic experiments and for the discovery of genes that modulate mutant phenotypes. ..
  10. doi Kcna1-mutant rats dominantly display myokymia, neuromyotonia and spontaneous epileptic seizures
    Saeko Ishida
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Brain Res 1435:154-66. 2012
    ..ADMS rats provide a new model, distinct from previously reported mouse models, for studying the diverse functions of Kv1.1 in vivo, as well as for understanding the pathology of EA1...
  11. ncbi National BioResource Project-Rat and related activities
    Tadao Serikawa
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University
    Exp Anim 58:333-41. 2009
    ..The future direction of rat resources are also discussed...
  12. ncbi Kyoto rhino rats derived by ENU mutagenesis undergo congenital hair loss and exhibit focal glomerulosclerosis
    Takashi Kuramoto
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Exp Anim 60:57-63. 2011
    ..The F344-Hr(krh) rat is a useful model of skin disease and may provide a new model system for the examination of the pathogenesis of FGS...
  13. doi An informative set of SSLP markers and genomic profiles in the rat MHC, the RT1 complex
    Yumie Takagi
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe cho Sakyo ku, Kyoto 606 8501, Japan
    Immunogenetics 61:189-97. 2009
    ..Owing to the accuracy and ease of determination, PCR-based SSLP genotyping could replace serological typing in genetic analyses and characterization of rat major histocompatibility...
  14. doi Fat Mass Reduction With Adipocyte Hypertrophy and Insulin Resistance in Heterozygous PPARγ Mutant Rats
    Valentino Gumbilai
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Diabetes 65:2954-65. 2016
    ..Furthermore, the study indicates that PPARγ regulates mainly adipocyte number rather than adipocyte size in vivo. The choice of appropriate species as experimental models is critical, especially for the study of PPARγ. ..
  15. doi Enhanced colitis-associated colon carcinogenesis in a novel Apc mutant rat
    Kazuto Yoshimi
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe cho, Sakyo ku, Kyoto, Japan
    Cancer Sci 100:2022-7. 2009
    ..To our knowledge, AOM/DSS-induced colon carcinogenesis using the KAD rat is the most efficient to induce colon tumors in the rat, and therefore would be available as an excellent model for human colitis-associated CRC...
  16. ncbi Rat Phenome Project: the untapped potential of existing rat strains
    Tomoji Mashimo
    Institute of Laboratory Animals, Kyoto University, Graduate School of Medicine, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    J Appl Physiol 98:371-9. 2005
    ..All deposited strains and obtained data are freely available for any interested researcher worldwide at http://www.anim.med.kyoto-u.ac.jp/nbr...
  17. doi Therapy for hyperthermia-induced seizures in Scn1a mutant rats
    Keiichiro Hayashi
    Department of Physiology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan
    Epilepsia 52:1010-7. 2011
    ..To assess the pharmacologic validity of this model, we compared the efficacies of eight different antiepileptic drugs (AEDs) for the treatment of hyperthermia-induced seizures using N1417H-Scn1a mutant rats...
  18. pmc ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
    Kazuto Yoshimi
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Nat Commun 7:10431. 2016
    ..2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms. ..
  19. pmc Advances on genetic rat models of epilepsy
    Tadao Serikawa
    Graduate School of Medicine, Kyoto University, Sakyo ku 606 8501 Laboratory of Pharmacology, Osaka University of Pharmaceutical Sciences, Takatsuki 569 1094, Japan
    Exp Anim 64:1-7. 2015
    ..In the near future, development of novel epileptic rat models will be extensively promoted by the use of sophisticated genome editing technologies. ..
  20. doi Methylphenidate improves learning impairments and hyperthermia-induced seizures caused by an Scn1a mutation
    Iori Ohmori
    Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Department of Medical Ethics, Graduate School and Faculty of Medicine, Kyoto University, Kyoto, Japan
    Epilepsia 55:1558-67. 2014
    ..Using a rodent model with an Scn1a mutation, we aimed to elucidate the pathophysiologic basis and potential therapeutic treatments for developmental disorders stemming from Scn1a mutations...
  21. pmc Simple knockout by electroporation of engineered endonucleases into intact rat embryos
    Takehito Kaneko
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Sci Rep 4:6382. 2014
    ..Electroporation technology provides a simple and effective method to produce knockout animals. ..
  22. doi Generation of leptin-deficient Lepmkyo/Lepmkyo rats and identification of leptin-responsive genes in the liver
    Megumi Aizawa-Abe
    Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, Sakyo ku, Kyoto, Japan
    Physiol Genomics 45:786-93. 2013
    ..Finally, we identified eight genes that primarily respond to leptin in the liver commonly in rat and mouse. These genes might be important for the effect of leptin in the liver. ..
  23. doi Evaluation of seizure foci and genes in the Lgi1(L385R/+) mutant rat
    Naohiro Fumoto
    Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Neurosci Res 80:69-75. 2014
    ..Finally, microarray analysis revealed a number of differentially expressed genes that may be involved in epilepsy. These results suggest that Lgi1(L385R/+) rats are useful as an animal model of human ADLTE. ..
  24. ncbi Comprehensive QTL analysis of serum cholesterol levels before and after a high-cholesterol diet in SHRSP
    Tomoji Mashimo
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Physiol Genomics 30:95-101. 2007
    ..This observation is significant in establishing a new model for atherosclerosis with hypertension in rats...
  25. pmc Simple Genome Editing of Rodent Intact Embryos by Electroporation
    Takehito Kaneko
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, 606 8501, Japan
    PLoS ONE 10:e0142755. 2015
    ..The TAKE method could be widely used as a powerful tool to produce genetically modified animals by genome editing. ..
  26. doi Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection
    Takehito Kaneko
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto, 606 8501, Japan
    Methods Mol Biol 1239:307-15. 2015
    ..This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9. ..