Shiro Kobayashi

Summary

Affiliation: Kyoto Institute of Technology
Country: Japan

Publications

  1. ncbi request reprint Chitinase-catalyzed synthesis of alternatingly N-deacetylated chitin: a chitin-chitosan hybrid polysaccharide
    Akira Makino
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 7:950-7. 2006
  2. pmc Lipase-catalyzed polyester synthesis--a green polymer chemistry
    Shiro Kobayashi
    R and D Center for Biobased Materials, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:338-65. 2010
  3. ncbi request reprint A hyaluronidase supercatalyst for the enzymatic polymerization to synthesize glycosaminoglycans
    Shiro Kobayashi
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Chemistry 12:5962-71. 2006
  4. doi request reprint Recent developments in lipase-catalyzed synthesis of polyesters
    Shiro Kobayashi
    R and D Center for Bio based materials, Kyoto Institute of Technology, Matsugasaki, Sakyo ku Kyoto 606 8585, Japan
    Macromol Rapid Commun 30:237-66. 2009
  5. ncbi request reprint Enzymatic polymerization to novel polysaccharides having a glucose-N-acetylglucosamine repeating unit, a cellulose-chitin hybrid polysaccharide
    Shiro Kobayashi
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 7:1644-56. 2006
  6. ncbi request reprint Enzymatic copolymerization to hybrid glycosaminoglycans: a novel strategy for intramolecular hybridization of polysaccharides
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 8:1802-6. 2007
  7. ncbi request reprint Chitinase-catalyzed synthesis of an alternatingly N-sulfonated chitin derivative
    Akira Makino
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 8:188-95. 2007
  8. ncbi request reprint Hyaluronidase-catalyzed copolymerization for the single-step synthesis of functionalized hyaluronan derivatives
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 8:1327-32. 2007
  9. ncbi request reprint Keratanase II-catalyzed synthesis of keratan sulfate oligomers by using sugar oxazolines as transition-state analogue substrate monomers: a novel insight into the enzymatic catalysis mechanism
    Masashi Ohmae
    Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Chembiochem 8:1710-20. 2007
  10. ncbi request reprint Enzymatic glycosidation of sugar oxazolines having a carboxylate group catalyzed by chitinase
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Carbohydr Res 339:2769-88. 2004

Collaborators

  • Masashi Ohmae
  • Yuichi Kimura
  • Hitomi Ohara
  • Hiroshi Uyama
  • Jun ichi Kadokawa
  • Mitsuyoshi Ueda
  • Hirofumi Ochiai
  • Akira Makino
  • Kiyoaki Ishimoto
  • Shin Ichiro Shoda
  • Shun Ichi Fujikawa
  • Masahiko Ishii
  • Naoya Yabuuchi
  • Maho Arimoto
  • Hirofumi Yamashita
  • Shunsaku Kimura
  • Masahiro Mishina
  • Takeji Hashimoto
  • Kazuhiro Kurosaki
  • Tomonori Mori
  • Itsuko Nakamura
  • Peng Xu
  • Tokuma Fukuoka
  • Yuji Aso
  • Syuhei Yamaguchi
  • Koji Morita
  • Tomoya Okuda
  • Kouji Morita
  • Keiichi Oda
  • Hideaki Nagashima
  • Yasuo Katayama
  • Kiichi Ishiwata
  • Mika Naganawa
  • Kenji Ishii
  • Hirokazu Tanaka
  • Satoshi Koizumi
  • Toshifumi Maeda
  • James E Whitten
  • Hisanari Yoneda
  • Junji Sugiyama
  • David L Kaplan
  • Yoichi Tachibana
  • Hiroyuki Tonami

Detail Information

Publications27

  1. ncbi request reprint Chitinase-catalyzed synthesis of alternatingly N-deacetylated chitin: a chitin-chitosan hybrid polysaccharide
    Akira Makino
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 7:950-7. 2006
    ..Molecular weights of 2 reached 2,020 with using chitinase from Serratia marcescens, which corresponds to 10-12 saccharide units...
  2. pmc Lipase-catalyzed polyester synthesis--a green polymer chemistry
    Shiro Kobayashi
    R and D Center for Biobased Materials, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:338-65. 2010
    ..The polymerization characteristics are of green nature currently demanded for sustainable society, and hence, desirable for conducting 'green polymer chemistry'...
  3. ncbi request reprint A hyaluronidase supercatalyst for the enzymatic polymerization to synthesize glycosaminoglycans
    Shiro Kobayashi
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Chemistry 12:5962-71. 2006
    ..Various sugar oxazoline monomers prepared based on the concept "transition-state analogue substrate" were successfully polymerized and copolymerized with HAase catalysis, yielding natural and unnatural glycosaminoglycans...
  4. doi request reprint Recent developments in lipase-catalyzed synthesis of polyesters
    Shiro Kobayashi
    R and D Center for Bio based materials, Kyoto Institute of Technology, Matsugasaki, Sakyo ku Kyoto 606 8585, Japan
    Macromol Rapid Commun 30:237-66. 2009
    ..Also, since lipase-catalyzed polyester synthesis provides a good opportunity for conducting "green polymer chemistry", the importance of this is described...
  5. ncbi request reprint Enzymatic polymerization to novel polysaccharides having a glucose-N-acetylglucosamine repeating unit, a cellulose-chitin hybrid polysaccharide
    Shiro Kobayashi
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 7:1644-56. 2006
    ..Furthermore, these unnatural hybrids of 2 and 4 were successfully digested by lysozyme from human neutrophils...
  6. ncbi request reprint Enzymatic copolymerization to hybrid glycosaminoglycans: a novel strategy for intramolecular hybridization of polysaccharides
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 8:1802-6. 2007
    ..These hybrid GAGs were successfully digested by the catalysis of hyaluronan lyase, clearly exhibiting that the products are not a blend of different homopolymers but an intramolecularly hybridized GAG...
  7. ncbi request reprint Chitinase-catalyzed synthesis of an alternatingly N-sulfonated chitin derivative
    Akira Makino
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 8:188-95. 2007
    ..It is considered that the C-2 position of the nonreducing unit in the oxazoline-type monomer is not deeply involved in the catalysis of chitinase...
  8. ncbi request reprint Hyaluronidase-catalyzed copolymerization for the single-step synthesis of functionalized hyaluronan derivatives
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 8:1327-32. 2007
    ..The composition of the N-acyl groups was controlled by varying the comonomer feed ratio. The copolymerization mechanism was also discussed...
  9. ncbi request reprint Keratanase II-catalyzed synthesis of keratan sulfate oligomers by using sugar oxazolines as transition-state analogue substrate monomers: a novel insight into the enzymatic catalysis mechanism
    Masashi Ohmae
    Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Chembiochem 8:1710-20. 2007
    ..This is the first report of KSase II-catalyzed transglycosylation to form beta(1-->3)-glycosidic bond through a substrate-assisted mechanism...
  10. ncbi request reprint Enzymatic glycosidation of sugar oxazolines having a carboxylate group catalyzed by chitinase
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Carbohydr Res 339:2769-88. 2004
    ....
  11. doi request reprint Biobased polymers: synthesis of graft copolymers and comb polymers using lactic acid macromonomer and properties of the product polymers
    Kiyoaki Ishimoto
    Department of Biobased Materials Science, Kyoto Institute of Technology, Matsugasaki, Kyoto 606 8585, Japan
    Biomacromolecules 13:3757-68. 2012
    ..In addition, the present approach provided fundamental information on relationships between the length of PLA side chain and the bulk properties of the product polymers...
  12. ncbi request reprint Enzymatic synthesis of chondroitin 4-sulfate with well-defined structure
    Shun Ichi Fujikawa
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 6:2935-42. 2005
    ..Compounds 1b and 1c were not catalyzed by the enzyme, affording the corresponding disaccharides through the oxazoline ring-opening without formation of polysaccharides...
  13. doi request reprint Optical resolution of n-butyl D- and L-lactates using immobilized lipase catalyst
    Hitomi Ohara
    R and D Center for Bio based materials, Kyoto Institute of Technology, Matsugasaki Sakyo ku, Kyoto 606 8585, Japan
    J Biosci Bioeng 111:19-21. 2011
    ..1)H-NMR showed that BuDLa reacted to oligomers, while BuLLa did not react. A mixture containing 90.4% of BuLLa and 9.6% of BuDLa was incubated with the enzyme for 72 h, then distilled. The purity of BuLLa increased to 98.6%...
  14. ncbi request reprint Synthesis of fluorinated chitin derivatives via enzymatic polymerization
    Akira Makino
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Macromol Biosci 6:862-72. 2006
    ..XRD measurements revealed that polysaccharides 2a and 2b had crystalline structures similar to that of alpha-chitin. [reaction: see text]..
  15. doi request reprint Lipase-catalyzed oligomerization and hydrolysis of alkyl lactates: direct evidence in the catalysis mechanism that enantioselection is governed by a deacylation step
    Hitomi Ohara
    R and D Center for Bio based materials, Department of Bio Based Materials Science, and Center for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Biomacromolecules 11:2008-15. 2010
    ..In the co-oligomerization between l- and d-lactates, the l-isomer retarded the reaction rate of the d-isomer, which was found due to the function of the former as a competitive inhibitor in the acylation step toward the latter...
  16. ncbi request reprint Bottom-up synthesis of hyaluronan and its derivatives via enzymatic polymerization: direct incorporation of an amido functional group
    Hirofumi Ochiai
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    Biomacromolecules 6:1068-84. 2005
    ..The 2-isopropyl oxazoline derivative provided the N-isobutyryl derivative of HA in low yields. Monomers of 2-phenyl and 2-isopropenyl oxazoline derivatives were not polymerized. The mechanism of the polymerization is discussed...
  17. ncbi request reprint Enzymatic polymerization behavior using cellulose-binding domain deficient endoglucanase II
    Itsuko Nakamura
    Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku Katsura, Nishikyo ku, Kyoto 615 8510, Japan
    Macromol Biosci 5:623-8. 2005
    ..EGII(core) is therefore advantageous for enzymatic polymerization...
  18. ncbi request reprint Enzymatic synthesis of chondroitin and its derivatives catalyzed by hyaluronidase
    Shiro Kobayashi
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    J Am Chem Soc 125:14357-69. 2003
    ..The M(n) value of 2a corresponds to that of the naturally occurring chondroitin. Thus, hyaluronidase catalysis allows the in vitro production of not only natural type but also the formation of unnatural type chondroitins...
  19. doi request reprint Enzymes as Green Catalysts for Precision Macromolecular Synthesis
    Shin Ichiro Shoda
    Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba ku, Sendai 980 8579, Japan
    Chem Rev 116:2307-413. 2016
    ..Thus, the enzymatic polymerization is desirable for the environment and contributes to "green polymer chemistry" for maintaining sustainable society. ..
  20. doi request reprint Biobased polymer system: miniemulsion of poly(alkyl methacrylate-graft-lactic acid)s
    Kiyoaki Ishimoto
    R and D Center for Bio based materials, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
    Biomacromolecules 10:2719-23. 2009
    ..The copolymer showed elastic polymer properties. The miniemulsion system may find useful applications as a new biobased polymer material that is environmentally desirable...
  21. ncbi request reprint Enzymatic synthesis and curing of biodegradable epoxide-containing polyesters from renewable resources
    Hiroshi Uyama
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Japan
    Biomacromolecules 4:211-5. 2003
    ..The obtained film showed good biodegradability, evaluated by BOD measurement in an activated sludge...
  22. ncbi request reprint Enzymatic polymerization of tyrosine derivatives. Peroxidase- and protease-catalyzed synthesis of poly(tyrosine)s with different structures
    Tokuma Fukuoka
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 606 8501, Japan
    Biomacromolecules 3:768-74. 2002
    ..Scanning electron microscopy observation of the polymer after 72 h showed the formation of a globular crystal in a diameter larger than 50 microm, which was not found by recrystallization of poly(tyrosine)...
  23. ncbi request reprint Peroxidase-catalyzed oxidative polymerization of bisphenols
    Hiroshi Uyama
    Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 606 8501, Japan
    Biomacromolecules 3:187-93. 2002
    ..Polymers from dihydroxydiphenylmethanes showed relatively high thermal stability...
  24. ncbi request reprint Evaluation of distribution of adenosine A2A receptors in normal human brain measured with [11C]TMSX PET
    Masahiro Mishina
    Neurological Institute, Nippon Medical School Chiba Hokusoh Hospital, Imba gun, Chiba ken 270 1694, Japan
    Synapse 61:778-84. 2007
    ..To date, [(11)C]TMSX is the only promising PET ligand, which is available to clinical use for mapping the A2ARs in the living human brain...
  25. ncbi request reprint Chemical reaction at specific sites and reaction-induced self-assembly as observed by in situ and real time SANS: enzymatic polymerization to synthetic cellulose
    Takeji Hashimoto
    Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319 1195, Japan
    Biomacromolecules 7:2479-82. 2006
    ..iii) The fibrils formed the aggregates, which had surface fractal dimension D(s) increasing from 2 to 2.3 with the reaction time, on the smooth surface of the enzyme aggregates...
  26. ncbi request reprint Peroxidase-catalyzed in situ polymerization of surface orientated caffeic acid
    Peng Xu
    Department of Biomedical Engineering, Bioengineering and Biotechnology Center, Tufts University, Medford, Massachusetts 02155, USA
    J Am Chem Soc 127:11745-53. 2005
    ..The results of this study expand the application of DPN technology to surface modification and surface chemistry reactions wherein stereo-regularity and regioselectivity can be exploited...