Fusako Kawai

Summary

Affiliation: Kyoto Institute of Technology
Country: Japan

Publications

  1. ncbi request reprint Biochemistry of microbial polyvinyl alcohol degradation
    Fusako Kawai
    R and D Center for Bio based materials, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Appl Microbiol Biotechnol 84:227-37. 2009
  2. ncbi request reprint The biochemistry and molecular biology of xenobiotic polymer degradation by microorganisms
    Fusako Kawai
    Research Institute for Bioresources, Okayama University, Okayama, Japan
    Biosci Biotechnol Biochem 74:1743-59. 2010
  3. ncbi request reprint A novel Ca²⁺-activated, thermostabilized polyesterase capable of hydrolyzing polyethylene terephthalate from Saccharomonospora viridis AHK190
    Fusako Kawai
    Center for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Appl Microbiol Biotechnol 98:10053-64. 2014
  4. ncbi request reprint Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them
    Fusako Kawai
    Center for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Kyoto 606 8585, Japan
    Arch Microbiol 195:131-40. 2013
  5. pmc Catalytic Mechanism of Short Ethoxy Chain Nonylphenol Dehydrogenase Belonging to a Polyethylene Glycol Dehydrogenase Group in the GMC Oxidoreductase Family
    Xin Liu
    Urban and Environmental Science College, Liaoning Normal University, Dalian 116029, China
    Int J Mol Sci 14:1218-31. 2013
  6. ncbi request reprint Involvement of PEG-carboxylate dehydrogenase and glutathione S-transferase in PEG metabolism by Sphingopyxis macrogoltabida strain 103
    Peechapack Somyoonsap
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama, 710 0046, Japan
    Appl Microbiol Biotechnol 81:473-84. 2008
  7. ncbi request reprint Isolation of bacteria able to grow on both polyethylene glycol (PEG) and polypropylene glycol (PPG) and their PEG/PPG dehydrogenases
    Xiaoping Hu
    Laboratory of Applied Microbiology, Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama, 710 0046, Japan
    Appl Microbiol Biotechnol 73:1407-13. 2007
  8. ncbi request reprint Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads
    Akio Tani
    Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki 710 0046, Okayama, Japan
    Microbiology 153:338-46. 2007
  9. ncbi request reprint Dual regulation of a polyethylene glycol degradative operon by AraC-type and GalR-type regulators in Sphingopyxis macrogoltabida strain 103
    Jittima Charoenpanich
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama 710 0046, Japan
    Microbiology 152:3025-34. 2006
  10. pmc Analysis of amino acid residues involved in catalysis of polyethylene glycol dehydrogenase from Sphingopyxis terrae, using three-dimensional molecular modeling-based kinetic characterization of mutants
    Takeshi Ohta
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama 710 0046, Japan
    Appl Environ Microbiol 72:4388-96. 2006

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Biochemistry of microbial polyvinyl alcohol degradation
    Fusako Kawai
    R and D Center for Bio based materials, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Appl Microbiol Biotechnol 84:227-37. 2009
    ..The complete pva operon was identified in Sphingopyxis sp. 113P3. Anaerobic biodegradability of PVA has also been suggested...
  2. ncbi request reprint The biochemistry and molecular biology of xenobiotic polymer degradation by microorganisms
    Fusako Kawai
    Research Institute for Bioresources, Okayama University, Okayama, Japan
    Biosci Biotechnol Biochem 74:1743-59. 2010
    ....
  3. ncbi request reprint A novel Ca²⁺-activated, thermostabilized polyesterase capable of hydrolyzing polyethylene terephthalate from Saccharomonospora viridis AHK190
    Fusako Kawai
    Center for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Appl Microbiol Biotechnol 98:10053-64. 2014
    ..Thus, the Cut190 will be a useful tool to elucidate the molecular mechanisms of the PET degradation, Ca(2+) activation, and stabilization...
  4. ncbi request reprint Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them
    Fusako Kawai
    Center for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Kyoto 606 8585, Japan
    Arch Microbiol 195:131-40. 2013
    ....
  5. pmc Catalytic Mechanism of Short Ethoxy Chain Nonylphenol Dehydrogenase Belonging to a Polyethylene Glycol Dehydrogenase Group in the GMC Oxidoreductase Family
    Xin Liu
    Urban and Environmental Science College, Liaoning Normal University, Dalian 116029, China
    Int J Mol Sci 14:1218-31. 2013
    ..Asn507 mediates the transfer of proton from a substrate to FAD and His465 transfers the same proton from the reduced flavin to an electron acceptor...
  6. ncbi request reprint Involvement of PEG-carboxylate dehydrogenase and glutathione S-transferase in PEG metabolism by Sphingopyxis macrogoltabida strain 103
    Peechapack Somyoonsap
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama, 710 0046, Japan
    Appl Microbiol Biotechnol 81:473-84. 2008
    ..GST activity was noncompetitively inhibited by acyl-CoA and PEG-carboxylate-CoA, suggesting the interaction of GST with them. The proposed role for GST is to buffer the toxicity of PEG-carboxylate-CoA...
  7. ncbi request reprint Isolation of bacteria able to grow on both polyethylene glycol (PEG) and polypropylene glycol (PPG) and their PEG/PPG dehydrogenases
    Xiaoping Hu
    Laboratory of Applied Microbiology, Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama, 710 0046, Japan
    Appl Microbiol Biotechnol 73:1407-13. 2007
    ..2006). PCR products of the five strains did not have similarity to each other or to oxidoreductases including PVA-DH...
  8. ncbi request reprint Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads
    Akio Tani
    Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki 710 0046, Okayama, Japan
    Microbiology 153:338-46. 2007
    ..These results suggest that the peg genes have evolved in a plasmid-mediated manner. An insertion of a transposon gene (pegF) between pegD and pegA in strain 203 was found, which caused the constitutive expression of pegA in this strain...
  9. ncbi request reprint Dual regulation of a polyethylene glycol degradative operon by AraC-type and GalR-type regulators in Sphingopyxis macrogoltabida strain 103
    Jittima Charoenpanich
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama 710 0046, Japan
    Microbiology 152:3025-34. 2006
    ....
  10. pmc Analysis of amino acid residues involved in catalysis of polyethylene glycol dehydrogenase from Sphingopyxis terrae, using three-dimensional molecular modeling-based kinetic characterization of mutants
    Takeshi Ohta
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama 710 0046, Japan
    Appl Environ Microbiol 72:4388-96. 2006
    ..Thus, we propose several important amino acid residues involved in the electron transfer pathway from the substrate to ubiquinone...
  11. ncbi request reprint Characterization of a cryptic plasmid, pSM103mini, from polyethylene-glycol degrading Sphingopyxis macrogoltabida strain 103
    Akio Tani
    Institute of Plant Science and Resources, Okayama University, Okayama, Japan
    Biosci Biotechnol Biochem 75:295-8. 2011
    ..The plasmid constructed was maintained in strain 103 and its close relative, S. macrogoltabida strain 203, but not efficiently in PEG-degrading S. terrae...
  12. ncbi request reprint Xenoestrogenic short ethoxy chain nonylphenol is oxidized by a flavoprotein alcohol dehydrogenase from Ensifer sp. strain AS08
    Xin Liu
    Laboratory of Applied Microbiology, Research Institute for Bioresources, Okayama University, Kurashiki, 2 20 1 Chuo, Kurashiki, 710 0046, Japan
    Appl Microbiol Biotechnol 73:1414-22. 2007
    ..0) and its components, and NPEOav10, but only slight or no activity was found using diethylene glycol, triethylene glycol, and PEG200...
  13. ncbi request reprint Biochemical and molecular characterization of a periplasmic hydrolase for oxidized polyvinyl alcohol from Sphingomonas sp. strain 113P3
    Wilailak Klomklang
    Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710 0046, Japan
    Microbiology 151:1255-62. 2005
    ..The oph gene was expressed in Escherichia coli under the control of the lac promoter. The recombinant protein had the same molecular mass and N-terminal amino acid sequence as the purified OPH from strain 113P3...
  14. ncbi request reprint Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103
    Akio Tani
    Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama 710 0046, Japan
    Arch Microbiol 189:407-10. 2008
    ....
  15. ncbi request reprint Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3
    Xiaoping Hu
    Laboratory of Applied Microbiology, Research Institute for Bioresources, Okayama University, 2 20 1 Chuo, Kurashiki, Okayama 710 0046, Japan
    Arch Microbiol 188:235-41. 2007
    ..No binding was found with PVA-grown cells treated with formaldehyde. Thus, a dent on the cell surface seems to be related to the uptake of PVA...
  16. ncbi request reprint Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119
    Uschara Thumarat
    Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, Japan
    Appl Microbiol Biotechnol 95:419-30. 2012
    ..The mutant was further activated by 2.6-fold (299 u/mg) with 300 mM Ca(2+) and was stable up to 60 °C with 150 mM Ca(2+). Another identical gene was located in tandem in the upstream of est119...
  17. ncbi request reprint Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis
    Akio Tani
    Research Institute for Bioresources, Okayama University, 2 20 1, Chuo, Kurashiki, Okayama, 710 0046, Japan
    J Biosci Bioeng 109:453-8. 2010
    ..Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance...
  18. ncbi request reprint Flow cytometry-based method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl
    Sou Iijima
    Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710 0046, Japan
    J Biosci Bioeng 109:645-51. 2010
    ..3%. The method we present here serves as a basic technique for the specific and direct isolation of live bacterial cells which contain dioxygenases active on dihydroxylated aromatic compounds...
  19. ncbi request reprint Metabolic pathway of xenoestrogenic short ethoxy chain-nonylphenol to nonylphenol by aerobic bacteria, Ensifer sp. strain AS08 and Pseudomonas sp. strain AS90
    Xin Liu
    Laboratory of Applied Microbiology, Research Institute for Bioresources, Okayama University, Kurashiki, 2 20 1 Chuo, Kurashiki 710 0046, Japan
    Appl Microbiol Biotechnol 72:552-9. 2006
    ..This process is repeated until NP is produced...
  20. ncbi request reprint The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis
    Akio Tani
    Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, Japan
    Microbiology 154:3437-46. 2008
    ....
  21. ncbi request reprint Analytical and computational techniques for exogenous depolymerization of xenobiotic polymers
    Masaji Watanabe
    Faculty of Environmental Science and Technology, Okayama University, 1 1 Naka 3 Chome, Tsushima, Okayama 700 8530, Japan
    Math Biosci 192:19-37. 2004
    ..AK-3. In addition, we present a numerical result which we obtained by applying the degradation rate based on the three week cultivation to simulate the transition of the weight distribution after cultivation of the fungus for five weeks...