Haruhisa Inoue

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. pmc Proteasome impairment in neural cells derived from HMSN-P patient iPSCs.
    Nagahisa Murakami
    Mol Brain 10:7. 2017
  2. pmc P301S mutant human tau transgenic mice manifest early symptoms of human tauopathies with dementia and altered sensorimotor gating
    Hiroki Takeuchi
    Department of Neurology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
    PLoS ONE 6:e21050. 2011
  3. pmc Anti-Aβ drug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer's disease
    Naoki Yahata
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e25788. 2011
  4. doi request reprint Neurodegenerative disease-specific induced pluripotent stem cell research
    Haruhisa Inoue
    Center for iPS Cell Research and Application, Kyoto University, 53, Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Exp Cell Res 316:2560-4. 2010
  5. doi request reprint A chemical neurotoxin, MPTP induces Parkinson's disease like phenotype, movement disorders and persistent loss of dopamine neurons in medaka fish
    Hideaki Matsui
    Department of Neurology, Kyoto University, Graduate School of Medicine, Kyoto 606 8507, Japan
    Neurosci Res 65:263-71. 2009
  6. doi request reprint Proteasome inhibition in medaka brain induces the features of Parkinson's disease
    Hideaki Matsui
    Department of Neurology, Kyoto University, Graduate School of Medicine, Sakyo ku, Kyoto, Japan
    J Neurochem 115:178-87. 2010
  7. doi request reprint Response to Comment on "Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells"
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 5:188lr2. 2013
  8. pmc Calcium dysregulation contributes to neurodegeneration in FTLD patient iPSC-derived neurons
    Keiko Imamura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Sci Rep 6:34904. 2016
  9. doi request reprint Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Aβ and differential drug responsiveness
    Takayuki Kondo
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 12:487-96. 2013
  10. pmc Amyotrophic lateral sclerosis model derived from human embryonic stem cells overexpressing mutant superoxide dismutase 1
    Tamaki Wada
    Stem Cell and Drug Discovery Institute, Kyoto, Japan
    Stem Cells Transl Med 1:396-402. 2012

Detail Information

Publications30

  1. pmc Proteasome impairment in neural cells derived from HMSN-P patient iPSCs.
    Nagahisa Murakami
    Mol Brain 10:7. 2017
    ..Collectively, these results suggest that our cellular model with defects in cellular integrity including UPS impairments may lead to identification of pathomechanisms and a therapeutic target for HMSN-P...
  2. pmc P301S mutant human tau transgenic mice manifest early symptoms of human tauopathies with dementia and altered sensorimotor gating
    Hiroki Takeuchi
    Department of Neurology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
    PLoS ONE 6:e21050. 2011
    ..Therefore, assessment of sensorimotor gating could be used to detect the earliest manifestations of tauopathies exemplified by prodromal AD, in which abnormal tau protein may play critical roles in the onset of neuronal dysfunctions...
  3. pmc Anti-Aβ drug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer's disease
    Naoki Yahata
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e25788. 2011
    ..Induced pluripotent stem (iPS) cells potentially provide an opportunity to generate a human cell-based model of AD that would be crucial for drug discovery as well as for investigating mechanisms of the disease...
  4. doi request reprint Neurodegenerative disease-specific induced pluripotent stem cell research
    Haruhisa Inoue
    Center for iPS Cell Research and Application, Kyoto University, 53, Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Exp Cell Res 316:2560-4. 2010
    ..Further progress in these areas of research can be made for currently incurable neurodegenerative diseases...
  5. doi request reprint A chemical neurotoxin, MPTP induces Parkinson's disease like phenotype, movement disorders and persistent loss of dopamine neurons in medaka fish
    Hideaki Matsui
    Department of Neurology, Kyoto University, Graduate School of Medicine, Kyoto 606 8507, Japan
    Neurosci Res 65:263-71. 2009
    ..Moreover, the amounts of dopamine persistently decreased in the brain of these MPTP-treated fish. MPTP-treated medaka is valuable for modeling human PD...
  6. doi request reprint Proteasome inhibition in medaka brain induces the features of Parkinson's disease
    Hideaki Matsui
    Department of Neurology, Kyoto University, Graduate School of Medicine, Sakyo ku, Kyoto, Japan
    J Neurochem 115:178-87. 2010
    ....
  7. doi request reprint Response to Comment on "Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells"
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 5:188lr2. 2013
    ..Our work and the study of Bilican et al. highlight the need for complementary assays to detect subtle phenotypic differences between control and mutant induced pluripotent stem cell lines...
  8. pmc Calcium dysregulation contributes to neurodegeneration in FTLD patient iPSC-derived neurons
    Keiko Imamura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Sci Rep 6:34904. 2016
    ..These data suggest that neuronal activity may regulate neurodegeneration in tauopathy. This FTLD-Tau model provides mechanistic insights into tauopathy pathogenesis and potential avenues for treatments...
  9. doi request reprint Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Aβ and differential drug responsiveness
    Takayuki Kondo
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Cell Stem Cell 12:487-96. 2013
    ..It also illustrates how patient-specific iPSCs can be useful for analyzing AD pathogenesis and evaluating drugs...
  10. pmc Amyotrophic lateral sclerosis model derived from human embryonic stem cells overexpressing mutant superoxide dismutase 1
    Tamaki Wada
    Stem Cell and Drug Discovery Institute, Kyoto, Japan
    Stem Cells Transl Med 1:396-402. 2012
    ..Thus, this model is expected to help unravel the disease mechanisms involved in the development of FALS and also lead to potential drug discoveries based on the prevention of neurodegeneration...
  11. doi request reprint Drug screening for ALS using patient-specific induced pluripotent stem cells
    Naohiro Egawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Transl Med 4:145ra104. 2012
    ..These findings suggest that motor neurons generated from ALS patient-derived iPSCs may provide a useful tool for elucidating ALS disease pathogenesis and for screening drug candidates...
  12. doi request reprint Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders
    Taizo Ishikawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, Japan
    Hum Mol Genet . 2016
    ..These results suggest that patient iPSCs with BH4 metabolism disorders provide an opportunity for screening substances for treating aberrant DA synthesis-related disorders...
  13. pmc Modeling Alexander disease with patient iPSCs reveals cellular and molecular pathology of astrocytes
    Takayuki Kondo
    Center for iPS Cell Research and Application CiRA, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, 606 8507, Japan
    Acta Neuropathol Commun 4:69. 2016
    ..Astrocytes, differentiated from induced pluripotent stem cells of Alexander disease, could be a cellular model for future translational medicine. ..
  14. pmc Motor neuron-specific disruption of proteasomes, but not autophagy, replicates amyotrophic lateral sclerosis
    Yoshitaka Tashiro
    Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto 6068507, Japan
    J Biol Chem 287:42984-94. 2012
    ..Enhancement of proteasome activity may be a promising strategy for the treatment of ALS...
  15. pmc N-cadherin regulates p38 MAPK signaling via association with JNK-associated leucine zipper protein: implications for neurodegeneration in Alzheimer disease
    Koichi Ando
    School of Human Health Sciences, Kyoto University Graduate School of Medicine, Kyoto 606 8507, Japan
    J Biol Chem 286:7619-28. 2011
    ..The dissociation of N-cadherin-mediated synaptic contact by Aβ may underlie the pathological basis of neurodegeneration such as neuronal death, synaptic loss, and Tau phosphorylation in Alzheimer disease brain...
  16. doi request reprint Cytoplasmic aggregates of dynactin in iPSC-derived tyrosine hydroxylase-positive neurons from a patient with Perry syndrome
    Takayasu Mishima
    Center for iPS Cell Research and Application CiRA, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, 606 8507, Japan Department of Neurology, Fukuoka University School of Medicine, 7 45 1 Nanakuma, Jonan ku, Fukuoka, 814 0180, Japan Electronic address
    Parkinsonism Relat Disord 30:67-72. 2016
    ..Eight mutations in DCTN1 gene have been reported. A novel disease model is required because the detailed pathogenesis remains unclear...
  17. doi request reprint α-Synuclein BAC transgenic mice as a model for Parkinson's disease manifested decreased anxiety-like behavior and hyperlocomotion
    Hodaka Yamakado
    Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Neurosci Res 73:173-7. 2012
    ..Our α-syn BAC tg mice could be a valuable tool to evaluate α-syn gene dosage effects in vivo...
  18. doi request reprint Loss of PINK1 in medaka fish (Oryzias latipes) causes late-onset decrease in spontaneous movement
    Hideaki Matsui
    Department of Neurology, Kyoto University, Graduate School of Medicine, 54 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Neurosci Res 66:151-61. 2010
    ..Thus, PINK1 contributes to the maintenance of dopamine metabolism, even before the selective death of dopaminergic neurons. Our animal model is therefore a valuable tool to detect pathogenesis in Parkinson's patients in the early stages...
  19. doi request reprint Bereitschaftspotential augmentation by neuro-feedback training in Parkinson's disease
    Tomoyuki Fumuro
    Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Clin Neurophysiol 124:1398-405. 2013
    ..We examined whether PD patients could increase BP amplitude by means of neuro-feedback (NFB) training for their slow cortical potentials (SCPs)...
  20. pmc The endoplasmic reticulum stress sensor, ATF6α, protects against neurotoxin-induced dopaminergic neuronal death
    Naohiro Egawa
    Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 286:7947-57. 2011
    ....
  21. pmc In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
    Kohei Yamamizu
    Laboratory of Stem Cell Differentiation, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application CiRA, Kyoto University, 53 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan Electronic address
    Stem Cell Reports 8:634-647. 2017
    ..This technology should be useful for research on human BBB physiology, pathology, and drug development...
  22. pmc Focal transplantation of human iPSC-derived glial-rich neural progenitors improves lifespan of ALS mice
    Takayuki Kondo
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan CREST, JST, Saitama 332 0012, Japan
    Stem Cell Reports 3:242-9. 2014
    ..Our data suggest a potential therapeutic mechanism via activation of AKT signal. The results demonstrated the efficacy of cell therapy for ALS by the use of human iPSCs as cell source...
  23. pmc Modeling the early phenotype at the neuromuscular junction of spinal muscular atrophy using patient-derived iPSCs
    Michiko Yoshida
    Department of Clinical Application, Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602 8566, Japan
    Stem Cell Reports 4:561-8. 2015
    ..Thus, the current in vitro model of AChR clustering using SMA patient-derived iPSCs is useful to dissect the pathophysiological mechanisms underlying the development of SMA, and to evaluate the efficacy of new therapeutic approaches. ..
  24. doi request reprint Chemical library screening identifies a small molecule that downregulates SOD1 transcription for drugs to treat amyotrophic lateral sclerosis
    Gaku Murakami
    Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Biomol Screen 16:405-14. 2011
    ..The assay developed here provides a powerful strategy for discovering novel lead molecules for treating familial SOD1-mediated ALS...
  25. doi request reprint Research on neurodegenerative diseases using induced pluripotent stem cells
    Keiko Imamura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Psychogeriatrics 12:115-9. 2012
    ..In this review, we discuss the recent advances in iPSC technology as well as its potential applications...
  26. doi request reprint Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives
    Fabian Oceguera-Yanez
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Methods 101:43-55. 2016
    ..Here, we outline our AAVS1 targeting protocol using standardized donor vectors and construction methods, as well as provide practical considerations for iPSC culture, drug selection, and genotyping. ..
  27. pmc Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells
    Masatoshi Kajiwara
    Center for Induced Pluripotent Stem Cell Research and Application, Department of Surgery, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 109:12538-43. 2012
    ..These data underscore the importance of donor differences when comparing the differentiation propensities of hiPSC clones...
  28. doi request reprint A chemical probe that labels human pluripotent stem cells
    Nao Hirata
    Institute for Integrated Cell Material Sciences WPI iCeMS, Kyoto University, Kyoto 606 8501, Japan Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Cell Rep 6:1165-74. 2014
    ..Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents. ..
  29. ncbi request reprint [ALS disease modeling and drug screening using patient-specific iPS cells]
    Naohiro Egawa
    Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
    Rinsho Shinkeigaku 53:1020-2. 2013
    ..Anacardic acid treatment reverted those phenotypes. Disease-specific iPSCs might provide a first step for drug-screening platform for ALS using patient-specific iPSCs. ..
  30. doi request reprint Nicotinic receptor stimulation protects nigral dopaminergic neurons in rotenone-induced Parkinson's disease models
    Hiroki Takeuchi
    Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Neurosci Res 87:576-85. 2009
    ..These results suggest that the rotenone mouse model may be useful for assessing candidate antiparkinson agents, and that nAChR (nicotinic acetylcholine receptor) stimulation can protect DA neurons against degeneration...