Shigeo Ikegawa

Summary

Affiliation: Kinki University
Country: Japan

Publications

  1. doi request reprint Simultaneous determination of twelve tetrahydrocorticosteroid glucuronides in human urine by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Anal Chem 81:10124-35. 2009
  2. doi request reprint Synthesis of 3- and 21-monosulfates of [2,2,3β,4,4-²H₅]-tetrahydrocorticosteroids in the 5β-series as internal standards for mass spectrometry
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Steroids 76:1232-40. 2011
  3. doi request reprint Immunoprecipitation and MALDI-MS identification of lithocholic acid-tagged proteins in liver of bile duct-ligated rats
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    J Lipid Res 49:2463-73. 2008
  4. ncbi request reprint Production and characterization of a monoclonal antibody to capture proteins tagged with lithocholic acid
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Anal Sci 24:1475-80. 2008
  5. doi request reprint Chemical synthesis of bile acid acyl-adenylates and formation by a rat liver microsomal fraction
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka, 577 8502, Japan
    Steroids 74:751-7. 2009
  6. doi request reprint Chemical synthesis of N-acetylcysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka 577 8502, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2630-8. 2009
  7. doi request reprint Synthesis of the 3-sulfates of N-acetylcysteine conjugated bile acids (BA-NACs) and their transient formation from BA-NACs and subsequent hydrolysis by a rat liver cytosolic fraction as shown by liquid chromatography/electrospray ionization-mass spectrome
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka, 577 8502, Japan
    Anal Bioanal Chem 400:2061-72. 2011
  8. doi request reprint Synthesis of the 3-sulfates of S-acyl glutathione conjugated bile acids and their biotransformation by a rat liver cytosolic fraction
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka, Japan
    Chem Phys Lipids 165:261-9. 2012
  9. doi request reprint Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka 577 8502, Japan
    Anal Biochem 384:224-30. 2009
  10. doi request reprint Identification of bile acid S-acyl glutathione conjugates in rat bile by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Steroids 76:68-77. 2011

Collaborators

Detail Information

Publications21

  1. doi request reprint Simultaneous determination of twelve tetrahydrocorticosteroid glucuronides in human urine by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Anal Chem 81:10124-35. 2009
    ..The method described here appears to be useful for clinical and biochemical studies...
  2. doi request reprint Synthesis of 3- and 21-monosulfates of [2,2,3β,4,4-²H₅]-tetrahydrocorticosteroids in the 5β-series as internal standards for mass spectrometry
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Steroids 76:1232-40. 2011
    ..The labeled compounds can be used as internal standards in liquid chromatography/mass spectrometry assays for clinical and biochemical studies...
  3. doi request reprint Immunoprecipitation and MALDI-MS identification of lithocholic acid-tagged proteins in liver of bile duct-ligated rats
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    J Lipid Res 49:2463-73. 2008
    ..The covalent binding of the Rab proteins with LCA may influence vesicular transport or binding of vesicles to their cognate membrane and may contribute to LCA-induced liver toxicity...
  4. ncbi request reprint Production and characterization of a monoclonal antibody to capture proteins tagged with lithocholic acid
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Anal Sci 24:1475-80. 2008
    ..The antibody will be useful for monitoring the generation, localization, and capture of proteins tagged with LCA, which may be the cause of LCA-induced toxicity...
  5. doi request reprint Chemical synthesis of bile acid acyl-adenylates and formation by a rat liver microsomal fraction
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka, 577 8502, Japan
    Steroids 74:751-7. 2009
    ..In cholestatic disease, such adenylate formation might lead to subsequent bile acid conjugation with glutathione or proteins...
  6. doi request reprint Chemical synthesis of N-acetylcysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka 577 8502, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2630-8. 2009
    ..Rapid hydrolysis of the BA-NAC conjugates by rabbit liver carboxylesterase was found, demonstrating the possible labile nature of the NAC conjugates formed in the liver...
  7. doi request reprint Synthesis of the 3-sulfates of N-acetylcysteine conjugated bile acids (BA-NACs) and their transient formation from BA-NACs and subsequent hydrolysis by a rat liver cytosolic fraction as shown by liquid chromatography/electrospray ionization-mass spectrome
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka, 577 8502, Japan
    Anal Bioanal Chem 400:2061-72. 2011
    ..Such sulfation was proportional to the hydrophobicity of the unconjugated bile acid. Thus, NAC conjugates of BAs as well as their C-3 sulfates if formed in vivo are rapidly hydrolyzed by cytosolic enzymes...
  8. doi request reprint Synthesis of the 3-sulfates of S-acyl glutathione conjugated bile acids and their biotransformation by a rat liver cytosolic fraction
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka, Japan
    Chem Phys Lipids 165:261-9. 2012
    ..Sulfation was proportional to the hydrophobicity of the unconjugated bile acid. Thus GSH conjugates of bile acids as well as their C-3 sulfates if formed in vivo are rapidly hydrolyzed by cytosolic enzymes...
  9. doi request reprint Formation and biliary excretion of glutathione conjugates of bile acids in the rat as shown by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka 577 8502, Japan
    Anal Biochem 384:224-30. 2009
    ....
  10. doi request reprint Identification of bile acid S-acyl glutathione conjugates in rat bile by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Steroids 76:68-77. 2011
    ..Our results indicate that BA-GSH conjugates are formed and excreted in bile in the healthy rat, although this novel mode of BA conjugation is a very minor pathway...
  11. doi request reprint An efficient synthesis of 7α,12α-dihydroxy-4-cholesten-3-one and its biological precursor 7α-hydroxy-4-cholesten-3-one: Key intermediates in bile acid biosynthesis
    Shoujiro Ogawa
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Steroids 78:927-37. 2013
    ....
  12. doi request reprint Synthesis of multiply deuterated 3- and 21-monosulfates of allo-tetrahydrocorticosteroids as internal standards for mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Steroids 77:1423-37. 2012
    ..The labeled compounds can be used as internal standards in liquid chromatography/mass spectrometry assays for clinical and biochemical studies...
  13. doi request reprint Identification of S-acyl glutathione conjugates of bile acids in human bile by means of LC/ESI-MS
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    Steroids 76:1609-14. 2011
    ..Our results indicate that BA-GSH conjugates are formed and excreted in human bile, at least in infants, although this novel mode of conjugation is a very minor pathway...
  14. ncbi request reprint Potential corticoid metabolites: chemical synthesis of 3- and 21-monosulfates and their double-conjugates of tetrahydrocorticosteroids in the 5alpha- and 5beta-series
    Rika Okihara
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka 577 8502, Japan
    Chem Pharm Bull (Tokyo) 58:344-53. 2010
    ....
  15. doi request reprint Characterization of non-enzymatic acylation of amino or thiol groups of bionucleophiles by the acyl-adenylate or acyl-CoA thioester of cholic acid
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka, 577 8502, Japan
    Anal Bioanal Chem 400:2253-9. 2011
    ....
  16. ncbi request reprint Analysis of bile acid glutathione thioesters by liquid chromatography/electrospray ionization-tandem mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, Higashi, Osaka, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 855:88-97. 2007
    ..0, and was definitely identified by CID spectra by comparison with those of the authentic sample. The results show that both cholyl-adenylate and cholyl-CoA thioester are able to acylate GSH in vitro...
  17. doi request reprint Simultaneous determination of 18 tetrahydrocorticosteroid sulfates in human urine by liquid chromatography/electrospray ionization-tandem mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan Electronic address
    Steroids 85:18-29. 2014
    ..Thus, we have developed a sensitive, precise and accurate assay for urinary tetrahydrocorticosteroid sulfates that should be useful for clinical and biological studies...
  18. ncbi request reprint Enantioselective immunorecognition of protein modification with optically active ibuprofen using polyclonal antibody
    Hiromi Ito
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka 577 8502, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 806:11-7. 2004
    ..These results indicate that the developed method will be useful for monitoring the generation and localization of protein covalently bound with (S)-ibuprofen, which may be the cause of ibuprofen-induced toxicity...
  19. ncbi request reprint Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer
    Shunji Ishiwata
    Department of Pharmaceutical Sciences, Tohoku University Hospital, 1 1 Seiryo machi, Aoba ku, Sendai 980 8574, Japan
    Cancer Lett 212:113-9. 2004
    ..A marked positive staining of cancer cells in colon tissues was observed, and the subcellular staining pattern was mainly cytosolic. These data suggest that the role of TGT60kD in colon carcinogenesis...
  20. ncbi request reprint [Detailed characterization of bile acid and glucocorticoid world by mass spectrometry]
    Shigeo Ikegawa
    Faculty of Pharmaceutical Sciences, Kinki University, 3 4 1 Kowakae, Higashi Osaka, Osaka 577 8502, Japan
    Yakugaku Zasshi 133:661-79. 2013
    ....
  21. ncbi request reprint LC/MS/MS of steroids having vicinal diol as electrospray-active boronates
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan
    Chem Pharm Bull (Tokyo) 61:326-32. 2013
    ....