Manabu Ohyama

Summary

Affiliation: Keio University
Country: Japan

Publications

  1. doi request reprint Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Methods Mol Biol 879:389-401. 2012
  2. ncbi request reprint William J. Cunliffe Scientific Awards. Advances in the study of stem-cell-enriched hair follicle bulge cells: a review featuring characterization and isolation of human bulge cells
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Dermatology 214:342-51. 2007
  3. ncbi request reprint Immunologic and histopathologic characterization of an active disease mouse model for pemphigus vulgaris
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 118:199-204. 2002
  4. doi request reprint The mesenchymal component of hair follicle neogenesis: background, methods and molecular characterization
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Exp Dermatol 19:89-99. 2010
  5. ncbi request reprint Hair follicle bulge: a fascinating reservoir of epithelial stem cells
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 46:81-9. 2007
  6. doi request reprint Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo
    Ophelia Veraitch
    Department of Dermatology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 133:1479-88. 2013
  7. ncbi request reprint Pathogenic autoantibody production requires loss of tolerance against desmoglein 3 in both T and B cells in experimental pemphigus vulgaris
    Kazuyuki Tsunoda
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Eur J Immunol 32:627-33. 2002
  8. ncbi request reprint Suppression of the immune response against exogenous desmoglein 3 in desmoglein 3 knockout mice: an implication for gene therapy
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 120:610-5. 2003
  9. ncbi request reprint Conformational epitope mapping of antibodies against desmoglein 3 in experimental murine pemphigus vulgaris
    Hidemi Anzai
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 35:133-42. 2004
  10. ncbi request reprint Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3
    Kazuyuki Tsunoda
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Immunol 170:2170-8. 2003

Detail Information

Publications90

  1. doi request reprint Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Methods Mol Biol 879:389-401. 2012
    ..Finally, hair reconstitution assay is available for the assessment of multipotency in vivo and sets a basis for tissue engineering of hair follicles...
  2. ncbi request reprint William J. Cunliffe Scientific Awards. Advances in the study of stem-cell-enriched hair follicle bulge cells: a review featuring characterization and isolation of human bulge cells
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Dermatology 214:342-51. 2007
    ..In this review, recent advances in hair follicle bulge cell research are summarized, especially focusing on the characterization and isolation of human bulge cells...
  3. ncbi request reprint Immunologic and histopathologic characterization of an active disease mouse model for pemphigus vulgaris
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 118:199-204. 2002
    ..Pemphigus vulgaris model mice reflect several of the histopathologic and immunologic features seen in pemphigus vulgaris patients, and provide a valuable tool to investigate the pathophysiologic mechanisms of pemphigus vulgaris...
  4. doi request reprint The mesenchymal component of hair follicle neogenesis: background, methods and molecular characterization
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Exp Dermatol 19:89-99. 2010
    ..A list of trichogenic molecular markers identified by those assays is also provided. Finally, this methods review is completed by defining some of the major questions needing resolution...
  5. ncbi request reprint Hair follicle bulge: a fascinating reservoir of epithelial stem cells
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 46:81-9. 2007
    ..Additional clinical relevance of bulge cell biology includes the importance of bulge cells as a gene therapy target and their possible roles in tumorigenesis...
  6. doi request reprint Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo
    Ophelia Veraitch
    Department of Dermatology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 133:1479-88. 2013
    ..The current study suggests a, to our knowledge, previously unrecognized advantage of using hiPSCs to enhance epithelial-mesenchymal interactions in HF bioengineering...
  7. ncbi request reprint Pathogenic autoantibody production requires loss of tolerance against desmoglein 3 in both T and B cells in experimental pemphigus vulgaris
    Kazuyuki Tsunoda
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Eur J Immunol 32:627-33. 2002
    ..These results suggest that loss of tolerance against Dsg3 in both B and T cells is important for the development of autoimmune state of PV...
  8. ncbi request reprint Suppression of the immune response against exogenous desmoglein 3 in desmoglein 3 knockout mice: an implication for gene therapy
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 120:610-5. 2003
    ....
  9. ncbi request reprint Conformational epitope mapping of antibodies against desmoglein 3 in experimental murine pemphigus vulgaris
    Hidemi Anzai
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 35:133-42. 2004
    ..We have recently developed an active disease mouse model for PV by adoptive transfer of splenocytes from immunized or naive Dsg3-/- mice into Rag2-/- recipient mice...
  10. ncbi request reprint Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3
    Kazuyuki Tsunoda
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Immunol 170:2170-8. 2003
    ..These findings demonstrate the pathogenic heterogeneity among anti-Dsg3 IgG Abs due to their epitopes, and suggest the direct inhibition of adhesive interaction of Dsg as an initial molecular event of blister formation in pemphigus...
  11. pmc Defining the pathogenic involvement of desmoglein 4 in pemphigus and staphylococcal scalded skin syndrome
    Takeshi Nagasaka
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Clin Invest 114:1484-92. 2004
    ..These findings suggest that Dsg4 may play a role other than adhesion and that the cross-reactivity of desmoglein autoantibodies should be factored into the framework of future studies of autoimmune mechanisms in pemphigus...
  12. ncbi request reprint IgG binds to desmoglein 3 in desmosomes and causes a desmosomal split without keratin retraction in a pemphigus mouse model
    Atsushi Shimizu
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 122:1145-53. 2004
    ..These findings indicate that anti-Dsg3 IgG antibodies can directly access Dsg3 present in desmosomes in vivo and cause the subsequent desmosome separation that leads to blister formation in PV...
  13. ncbi request reprint Ultrastructural changes in mice actively producing antibodies to desmoglein 3 parallel those in patients with pemphigus vulgaris
    Atsushi Shimizu
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Arch Dermatol Res 294:318-23. 2002
    ....
  14. doi request reprint Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells
    Tetsuro Kobayashi
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 130:1988-95. 2010
    ..Our findings demonstrate a unique strategy utilizing canine bulge cells to investigate human stem cell biology and intractable hair disorders that involve the bulge region...
  15. doi request reprint Epidermal structure created by canine hair follicle keratinocytes enriched with bulge cells in a three-dimensional skin equivalent model in vitro: implications for regenerative therapy of canine epidermis
    Tetsuro Kobayashi
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, Japan
    Vet Dermatol 24:77-83.e19-20. 2013
    ..This cell population might thus be an ideal source for generating the interfollicular epidermis in a canine skin equivalent...
  16. pmc Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin
    Keisuke Nagao
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Nat Immunol 13:744-52. 2012
    ..Pre-LCs failed to enter hairless skin in mice or humans, which establishes hair follicles as portals for LCs...
  17. doi request reprint Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions
    Tetsuro Kobayashi
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Vet Dermatol 22:414-22. 2011
    ..This study provides crucial information necessary for further optimization of culture conditions of canine DP...
  18. doi request reprint Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation
    Koji Nishifuji
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 59:184-91. 2010
    ..ETs are known to cleave specifically a single peptide bond in the extracellular domains 3 and 4 of desmoglein (Dsg) 1. However, the precise mechanisms underlying ET-induced epidermal blister formation remain poorly understood...
  19. ncbi request reprint Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism
    Takayuki Ota
    Department of Dermatology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Int Immunol 16:1487-95. 2004
    ..These results indicate that auto-reactive B cells against peripheral antigen (Dsg3) are able to develop in the presence of Dsg3 but are ignored by the immune system...
  20. ncbi request reprint In vitro keratinocyte dissociation assay for evaluation of the pathogenicity of anti-desmoglein 3 IgG autoantibodies in pemphigus vulgaris
    Ken Ishii
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 124:939-46. 2005
    ..These findings indicate that this dissociation assay will provide a simple and objective biological method to measure the pathogenic strength of pemphigus autoantibodies...
  21. doi request reprint Ectopic expression of epidermal antigens renders the lung a target organ in paraneoplastic pemphigus
    Tsuyoshi Hata
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Immunol 191:83-90. 2013
    ..These findings demonstrate that squamous metaplasia after pulmonary epithelial injury may play a crucial role in redirecting the skin-specific autoimmune reaction to the lungs in PNP. ..
  22. doi request reprint Restoration of the intrinsic properties of human dermal papilla in vitro
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Cell Sci 125:4114-25. 2012
    ..The study reported here revealed previously unreported molecular mechanisms contributing to human DP properties and describes a useful technique for the investigation of human DP biology and hair follicle bioengineering...
  23. ncbi request reprint Anti-desmoglein 3 (Dsg3) monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity
    Yukari Yamamoto
    Department of Dermatology, Gifu University School of Medicine, Gifu City 501 1194, Japan
    J Biol Chem 282:17866-76. 2007
    ....
  24. ncbi request reprint Synergistic pathogenic effects of combined mouse monoclonal anti-desmoglein 3 IgG antibodies on pemphigus vulgaris blister formation
    Hiroshi Kawasaki
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 126:2621-30. 2006
    ..These mAbs will provide a valuable tool to investigate the molecular mechanisms of blister formation, mimicking the effects of the polyclonal IgG antibodies found in patients...
  25. ncbi request reprint Novel system evaluating in vivo pathogenicity of desmoglein 3-reactive T cell clones using murine pemphigus vulgaris
    Hayato Takahashi
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Immunol 181:1526-35. 2008
    ..This strategy is useful for evaluating the effector function of autoreactive T cells involved in the pathogenesis of various autoimmune diseases...
  26. doi request reprint Subcellular localization of desmosomal components is different between desmoglein3 knockout mice and pemphigus vulgaris model mice
    Hitoshi Saito
    Department of Dermatology, Saitama Municipal Hospital, 2460 Mimuro, Midori ku, Saitama shi, Saitama 336 8522, Japan
    J Dermatol Sci 55:108-15. 2009
    ..The desmoglein 3 (Dsg3) knockout mouse and pemphigus vulgaris (PV) mouse model present a similar type of supra-basal acantholysis, even though the subcellular mechanism is considered to be completely different...
  27. ncbi request reprint Conformational epitope mapping and IgG subclass distribution of desmoglein 3 in paraneoplastic pemphigus
    Yuko Futei
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Am Acad Dermatol 49:1023-8. 2003
    ..It has been demonstrated that in PV, dominant epitopes reside in N-terminal adhesive regions of Dsg3 and that the dominant IgG subclass against Dsg3 is IgG4...
  28. doi request reprint Autoreactive B-cell elimination by pathogenic IgG specific for the same antigen: implications for peripheral tolerance
    Takayuki Ota
    Department of Dermatology, Keio University School of Medicine, Tokyo160 8582, Japan
    Int Immunol 20:1351-60. 2008
    ....
  29. ncbi request reprint Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation
    Yasushi Hanakawa
    Department of Dermatology, School of Medicine, Ehime University, Ehime, Japan
    J Invest Dermatol 119:1231-6. 2002
    ..Our findings provide biologic evidence that desmoglein 1 and desmoglein 3 play a different functional role in cell-cell adhesion of keratinocytes...
  30. ncbi request reprint [Loss of adhesive function of desmosomal cadherin and skin diseases in humans and animals]
    Koji Nishifuji
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Seikagaku 78:609-14. 2006
  31. doi request reprint Differential X Chromosome Inactivation Patterns during the Propagation of Human Induced Pluripotent Stem Cells
    Tomoko Andoh-Noda
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Keio J Med . 2017
    ..Careful evaluation of X chromosome inactivation in hiPSC clones, particularly in early passages, by methods such as HUMARA-MSP, is thus important when using patient-specific hiPSCs to model X-linked disease...
  32. doi request reprint Promise of human induced pluripotent stem cells in skin regeneration and investigation
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 134:605-9. 2014
    ..With their developmental plasticity, iPSCs may also enable the regeneration of skin appendages. The iPSC technology may provide novel remedies for intractable disorders, once key issues particularly, safety concerns, are cleared. ..
  33. pmc Mitochondrial dysfunction associated with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue
    Yoichi Imaizumi
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Tokyo 160 8582, Japan
    Mol Brain 5:35. 2012
    ..The familial form of PD, PARK2, is caused by mutations in the parkin gene. parkin-knockout mouse models show some abnormalities, but they do not fully recapitulate the pathophysiology of human PARK2...
  34. doi request reprint Brief report: requirement of TACE/ADAM17 for hair follicle bulge niche establishment
    Keisuke Nagao
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    Stem Cells 30:1781-5. 2012
    ..This study provides mechanistic implication for human TACE-deficiency and for hair abnormality caused by EGFR inhibitors...
  35. ncbi request reprint Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression
    Koji Nishifuji
    Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3 5 8 Saiwai cho, Fuchu, Tokyo 183 8509, Japan
    Vet Immunol Immunopathol 95:177-82. 2003
    ....
  36. ncbi request reprint [Autoimmune blistering diseases]
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine
    Nihon Rinsho 63:540-6. 2005
  37. doi request reprint Pemphigus mouse model as a tool to evaluate various immunosuppressive therapies
    Yujiro Takae
    Department of Dermatology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    Exp Dermatol 18:252-60. 2009
    ..Knowing the advantages and limitations of this model will provide an important foundation for the future evaluation and development of novel therapeutic strategies...
  38. doi request reprint Post-IID 2008 International Meeting on autoimmune bullous diseases
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 128:2135-7. 2008
  39. ncbi request reprint In vivo ultrastructural localization of the desmoglein 3 adhesive interface to the desmosome mid-line
    Atsushi Shimizu
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 124:984-9. 2005
    ..These results indicate that the N-terminal regions of Dsg3 from opposing cells interact at the dense mid-line of desmosomes where EC1 overlaps...
  40. doi request reprint The molecular logic of pemphigus and impetigo: the desmoglein story
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Vet Dermatol 20:308-12. 2009
    ....
  41. pmc Autoimmune and infectious skin diseases that target desmogleins
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:524-37. 2010
    ..Further investigation of desmoglein molecules will continue to provide insight into the unsolved pathophysiological mechanisms of diseases and aid in the development of novel therapeutic strategies with minimal side effects...
  42. doi request reprint Immune dysregulation of pemphigus in humans and mice
    Tomoaki Yokoyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol 37:205-13. 2010
    ..Thus, delicate balance between self-reactive lymphocytes and regulatory T cells may be a key element in determining whether individuals produce pathogenic antibodies and develop pemphigus phenotypes or not...
  43. ncbi request reprint Cloning of canine desmoglein 3 and immunoreactivity of serum antibodies in human and canine pemphigus vulgaris with its extracellular domains
    Koji Nishifuji
    Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3 5 8 Saiwaicho, Fuchu, Tokyo 183 8509, Japan
    J Dermatol Sci 32:181-91. 2003
    ..The autoimmune target in human PV has been identified as desmoglein (Dsg) 3, a desmosomal cell-cell adhesion molecule, whereas the autoimmune target in canine PV has not yet been identified clearly...
  44. ncbi request reprint Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid
    Mariko Yoshida
    Department of Dermatology, Kurume University School of Medicine, 67 Asahi Machi, Kurume, Fukuoka 830 0011, Japan
    J Dermatol Sci 41:21-30. 2006
    ..We produced different recombinant glutathione-S-transferase-fusion proteins, which roughly presented N-terminal domain, central rod domain and C-terminal domain of human BP230...
  45. ncbi request reprint No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases
    Takayuki Ota
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi Shinjuku ku, 160 8582, Tokyo, Japan
    J Dermatol Sci 32:137-41. 2003
    ..About 20-30% of patients with PNP develop fatal bronchiolitis obliterans, in which autoantibody-mediated injury is suspected because of in vivo IgG deposition on cell surfaces of bronchial epithelia...
  46. ncbi request reprint Comparative study of autoantigen profile between Colombian and Brazilian types of endemic pemphigus foliaceus by various biochemical and molecular biological techniques
    Yoshiko Hisamatsu
    Department of Dermatology, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830 0011, Japan
    J Dermatol Sci 32:33-41. 2003
    ..Our previous study suggested that Colombian EPF seemed to react various plakin family proteins, such as envoplakin, periplakin and BP230...
  47. ncbi request reprint Desmoglein as a target in autoimmunity and infection
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, USA
    J Am Acad Dermatol 48:244-52. 2003
    ..In SSSS, exfoliative toxin produced by Staphylococcus aureus specifically binds and cleaves desmoglein 1 with resultant blister formation at the identical site...
  48. ncbi request reprint Pemphigus as a paradigm of autoimmunity and cell adhesion
    Masayuki Amagai
    Department of Dermatology, School of Medicine, Keio University, Tokyo, Japan
    Keio J Med 51:133-9. 2002
    ..This model will be valuable not only for dissecting the cellular and molecular mechanisms in pathogenic antibody production but also for developing novel therapeutic strategies...
  49. ncbi request reprint Staphylococcal exfoliative toxin B specifically cleaves desmoglein 1
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 118:845-50. 2002
    ..These findings demonstrate that exfoliative toxin A and exfoliative toxin B cause blister formation in staphylococcal scalded skin syndrome and bullous impetigo by identical molecular pathophysiologic mechanisms...
  50. ncbi request reprint [Immunological tests: Anti desmoglein 1 and 3 antibody]
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine
    Nihon Rinsho 63:571-4. 2005
  51. doi request reprint Pemphigus vulgaris and its active disease mouse model
    Masayuki Amagai
    Department of Dermatology, School of Medicine, Keio University, Tokyo, Japan
    Curr Dir Autoimmun 10:167-81. 2008
    ....
  52. ncbi request reprint Non-pathogenic anti-desmoglein 3 IgG autoantibodies in Fogo Selvagem
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 126:1931-2. 2006
    ....
  53. ncbi request reprint Cicatricial pemphigoid of the oropharynx after allogeneic stem cell transplantation for relapsed follicular lymphoma
    Yoshinobu Aisa
    Division of Hematology, Department of Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
    Int J Hematol 82:266-9. 2005
    ..A diagnosis of cicatricial pemphigoid (CP) was made, and complete resolution of the CP was achieved with prednisolone therapy. The occurrence of autoimmune blistering diseases is rare after allogeneic SCT...
  54. ncbi request reprint Characterization of skin microbiota in patients with atopic dermatitis and in normal subjects using 16S rRNA gene-based comprehensive analysis
    Itaru Dekio
    Department of Dermatology, School of Medicine, Keio University, Shinjuku ku, Tokyo 160 8582, Japan
    J Med Microbiol 56:1675-83. 2007
    ....
  55. ncbi request reprint IgG autoantibodies directed against desmoglein 3 cause dissociation of keratinocytes in canine pemphigus vulgaris and paraneoplastic pemphigus
    Koji Nishifuji
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Tokyo 160 8582, Japan
    Vet Immunol Immunopathol 117:209-21. 2007
    ..The present study indicates for the first time that circulating anti-Dsg3 IgG antibodies capable of dissociating keratinocytes are present in dogs with PV and PNP...
  56. ncbi request reprint Cloning of swine desmoglein 1 and its direct proteolysis by Staphylococcus hyicus exfoliative toxins isolated from pigs with exudative epidermitis
    Koji Nishifuji
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Vet Dermatol 16:315-23. 2005
    ..Recognition and digestion of calcium-stabilized structure on the extracellular domains of swine Dsg1 by Exhs indicated that EE shares similar molecular pathophysiological mechanisms of intra-epidermal splitting with SSSS in humans...
  57. ncbi request reprint Staphylococcal exfoliative toxins: "molecular scissors" of bacteria that attack the cutaneous defense barrier in mammals
    Koji Nishifuji
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 49:21-31. 2008
    ..The species-specificity of staphylococcal exfoliative toxins to cleave Dsg1 in certain mammalian species is discussed...
  58. ncbi request reprint Abnormal keratin expression in circumscribed palmar hypokeratosis
    Akira Ishiko
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    J Am Acad Dermatol 57:285-91. 2007
    ..Circumscribed palmar or plantar hypokeratosis (CPH) is a rare skin disorder only recently described...
  59. doi request reprint Changeability of the fully methylated status of the 15q11.2 region in induced pluripotent stem cells derived from a patient with Prader-Willi syndrome
    Hironobu Okuno
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    Congenit Anom (Kyoto) . 2016
    ..The present data potentially opens a door to cell-based therapy for PWS patients and, possibly, patients with other disorders associated with genomic imprinting. This article is protected by copyright. All rights reserved...
  60. pmc Functional Neurons Generated from T Cell-Derived Induced Pluripotent Stem Cells for Neurological Disease Modeling
    Takuya Matsumoto
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan Institute for Innovation, Ajinomoto Co, Inc, Kawasaki ku, Kanagawa 210 8681, Japan
    Stem Cell Reports 6:422-35. 2016
    ..Therefore, we conclude that TiPSCs are a useful tool for modeling neurological diseases...
  61. doi request reprint Application of electron microscopic analysis and fluorescent in situ hybridization technique for the successful diagnosis of extraskeletal Ewing's sarcoma
    Takeru Funakoshi
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol 42:893-6. 2015
    ..Subsequent FISH analysis identified reciprocal translocation of the ESWR1 gene, enabling the final diagnosis of extraskeletal ES. This study provides useful information enabling the diagnosis of this uncommon soft tissue tumor. ..
  62. doi request reprint Strategies to enhance epithelial-mesenchymal interactions for human hair follicle bioengineering
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol Sci 70:78-87. 2013
    ..These approaches may not always synergistically intensify EMIs, however, step-by-step investigation probing optimal combinations should maximally enhance EMIs to achieve successful human hair follicle bioengineering...
  63. pmc Generation of human melanocytes from induced pluripotent stem cells
    Shigeki Ohta
    Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 6:e16182. 2011
    ..This in vitro differentiation system should prove useful for understanding human melanocyte biology and revealing the mechanism of various pigment cell disorders, including melanoma...
  64. ncbi request reprint BP180 ELISA using bacterial recombinant NC16a protein as a diagnostic and monitoring tool for bullous pemphigoid
    Masakazu Kobayashi
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 30:224-32. 2002
    ..These findings indicate that NC16a ELISA will be a valuable tool not only for the diagnosis of patients with BP but also for the monitoring of the disease activity...
  65. ncbi request reprint [Desmoglein, the target molecule in autoimmunity and infection]
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine
    Nihon Rinsho Meneki Gakkai Kaishi 29:325-33. 2006
    ..It is not clear why so many diseases are clustered in desmogleins, but there must be a reason for this. Studies on desmogleins will provide an important framework to understand the mysteries between autoimmunity and infection...
  66. ncbi request reprint Tolerance induction by the blockade of CD40/CD154 interaction in pemphigus vulgaris mouse model
    Miyo Aoki-Ota
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 126:105-13. 2006
    ....
  67. ncbi request reprint Paraneoplastic pemphigus associated with Castleman's disease and asymptomatic bronchiolitis obliterans
    Wataru Fujimoto
    Department of Dermatology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Eur J Dermatol 12:355-9. 2002
    ..The expiratory images of high resolution computed tomography showed air trapping, indicating the presence of asymptomatic but gradually progressive bronchiolitis obliterans...
  68. doi request reprint Genetic characterization of human Dsg3-specific B cells isolated by flow cytometry from the peripheral blood of patients with pemphigus vulgaris
    Jun Yamagami
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol Sci 52:98-107. 2008
    ..Pemphigus vulgaris (PV) is an autoimmune bullous disease caused by anti-desmoglein 3 (Dsg3) IgG autoantibodies; however, the Dsg3-specific B cells that produce anti-Dsg3 IgG are not well characterized...
  69. ncbi request reprint No activation of urokinase plasminogen activator by anti-desmoglein 3 monoclonal IgG antibodies in cultured human keratinocytes
    Yukari Yamamoto
    Department of Dermatology, Gifu University School of Medicine, Yanagido 1 1, Gifu City 501 1194, Japan
    J Dermatol Sci 47:119-25. 2007
    ....
  70. pmc Pathogenic epitopes of autoantibodies in pemphigus reside in the amino-terminal adhesive region of desmogleins which are unmasked by proteolytic processing of prosequence
    Mariko Yokouchi
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 129:2156-66. 2009
    ..These findings support the idea that at least some pathogenic pemphigus autoantibodies induce the loss of cell adhesion by directly binding the trans-interaction site of Dsgs...
  71. ncbi request reprint A single helper T cell clone is sufficient to commit polyclonal naive B cells to produce pathogenic IgG in experimental pemphigus vulgaris
    Hayato Takahashi
    Department of Dermatology, Division of Rheumatology, Keio University School of Medicine, Tokyo, Japan
    J Immunol 182:1740-5. 2009
    ..These findings provide an important framework for examining immunological mechanisms in Ab-mediated autoimmune diseases...
  72. doi request reprint LNGFR+THY-1+ human pluripotent stem cell-derived neural crest-like cells have the potential to develop into mesenchymal stem cells
    Takehito Ouchi
    Department of Dentistry and Oral Surgery, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    Differentiation 92:270-280. 2016
    ..These results suggest that LNGFR+THY-1+ cells identified following NCLC induction from ESCs/iPSCs shared similar potentials with multipotent MSCs...
  73. doi request reprint Exploring the biology of the nail: An intriguing but less-investigated skin appendage
    Masataka Saito
    Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan Electronic address
    J Dermatol Sci 79:187-93. 2015
    ..However, it is at least certain that the nail unit has a promising potential for the future of regenerative medicine. This review explores the biology of the nail organ by focusing on intriguing knowledge gained from recent studies. ..
  74. pmc The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa
    Tetsu Yoshida
    Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku 160 8582, Tokyo, Japan
    Mol Brain 7:45. 2014
    ....
  75. doi request reprint Primary cicatricial alopecia: recent advances in understanding and management
    Manabu Ohyama
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol 39:18-26. 2012
    ..Based on that hypothesis and observations, novel therapeutic approaches have been proposed, including the use of peroxisome proliferator-activated receptor-γ agonist for lichen planopilaris...
  76. ncbi request reprint A case of herpetiform pemphigus with anti-desmoglein 3 IgG autoantibodies
    Rieko Isogai
    Department of Dermatology, Kinki University School of Medicine, Osaka Sayama, Japan
    J Dermatol 31:407-10. 2004
    ..It remains to be clarified why the anti-Dsg3 IgG autoantibodies in this patient induced this unique features of HP, rather than the mucosal dominant type of pemphigus vulgaris...
  77. ncbi request reprint Cutaneous type pemphigus vulgaris: a rare clinical phenotype of pemphigus
    Kazue Yoshida
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Am Acad Dermatol 52:839-45. 2005
    ..This expression can be a transient phenotype that may develop from, or evolve into, other subtypes of pemphigus...
  78. ncbi request reprint T helper type 2-biased natural killer cell phenotype in patients with pemphigus vulgaris
    Hayato Takahashi
    Department of Dermatology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Invest Dermatol 127:324-30. 2007
    ..Together these findings suggest that NK cells contribute to a T helper type 2-biased immune response in PV patients through impaired IL-12 signaling and an upregulation of IL-10 and IL-5...
  79. doi request reprint Detailed clinicopathological characterization of progressive alopecia areata patients treated with i.v. corticosteroid pulse therapy toward optimization of inclusion criteria
    Misato Sato
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol 41:957-63. 2014
    ....
  80. doi request reprint Histopathological investigation of clinically non-affected perilesional scalp in alopecias detected unexpected spread of disease activities
    Emiko Watanabe-Okada
    Department of Dermatology, Keio University School of Medicine, Tokyo Division of Dermatology, Kawasaki Municipal Hospital, Kawasaki, Japan
    J Dermatol 41:802-7. 2014
    ....
  81. doi request reprint Follicular microhemorrhage: a unique dermoscopic sign for the detection of coexisting trichotillomania in alopecia areata
    Misaki Ise
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dermatol 41:518-20. 2014
    ..So far, we have detected FMH in four TT patients with moderate to severe AA. Although further accumulation of cases is necessary, FMH would be beneficial to dissect complicated pathophysiology of hair loss in AA patients with TT. ..
  82. doi request reprint Rheumatoid neutrophilic dermatosis with tense blister formation: a case report and review of the literature
    Yumi Fujio
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Australas J Dermatol 55:e12-4. 2014
    ..Of note, half of the cases were resistant to corticosteroids, as anti-neutrophil agents are reported to be effective. Accordingly, it is important to recognise this unusual manifestation for the timely initiation of appropriate therapy. ..
  83. doi request reprint Ichthyosis follicularis, alopecia, and photophobia syndrome: a case report and a pathological insight into pilosebaceous anomaly
    Mariko Kamo
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    Am J Dermatopathol 33:403-6. 2011
    ....
  84. ncbi request reprint A mystery of AHNAK/desmoyokin still goes on
    Masayuki Amagai
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Invest Dermatol 123:xiv-xv. 2004
  85. pmc Flaky tail mouse denotes human atopic dermatitis in the steady state and by topical application with Dermatophagoides pteronyssinus extract
    Catharina Sagita Moniaga
    Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Am J Pathol 176:2385-93. 2010
    ..These results suggest that the Flg(ft) mouse genotype has potential as an animal model of AD corresponding with filaggrin mutation in human AD...
  86. doi request reprint Sequence analysis of filaggrin gene by novel shotgun method in Japanese atopic dermatitis
    Takashi Sasaki
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Dermatol Sci 51:113-20. 2008
    ..This unique gene structure has hampered the precise DNA sequence determination...
  87. ncbi request reprint Rapid growth of malignant melanoma in pregnancy
    Tomotaka Sato
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Dtsch Dermatol Ges 6:126-9. 2008
    ..There are no standarized guidelines for treatment; each case requires an individualized approach. We review the literature and present an algorithm to aid in approaching such patients...
  88. doi request reprint Inflammatory plaque with peripheral nodules: a new specific finding of cutaneous polyarteritis nodosa
    Po Tak Chan
    Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
    J Am Acad Dermatol 60:320-5. 2009
    ..In conclusion, cutaneous polyarteritis nodosa can present as inflammatory plaques on the trunk and proximal extremities, and the presence of peripheral nodules around these plaques constitutes a useful clinical clue to its diagnosis...
  89. ncbi request reprint Pathogenic monoclonal antibody against desmoglein 3 augments desmoglein 3 and p38 MAPK phosphorylation in human squamous carcinoma cell line
    Yuki Kawasaki
    Department of Dermatology, Gifu University School of Medicine, Gifu, 501 1194, Japan
    Autoimmunity 39:587-90. 2006
    ..These results indicate that antibodies bind to Dsg3, but not other antigens, in the IgG fraction and can induce activation of signal transduction...
  90. doi request reprint Candida albicans infection delays duodenal ulcer healing in cysteamine-induced duodenal ulcers in rats
    Longxue Jin
    Department of Surgery, Keio University School of Medicine, Shinjuku ku, Tokyo, 160 8582, Japan
    Dig Dis Sci 53:2878-85. 2008
    ..Using a rat model, we have demonstrated that Candida infection can delay the wound healing process of duodenal ulcers by means of a low expression of VEGF-A and PCNA...