Kazutake Shimada

Summary

Affiliation: Kanazawa University
Country: Japan

Publications

  1. ncbi request reprint Development and application of electrospray-active derivatization reagents for hydroxysteroids
    Tadashi Nishio
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 44:786-95. 2007
  2. ncbi request reprint Liquid chromatography-tandem mass spectrometric method for the determination of salivary 25-hydroxyvitamin D3: a noninvasive tool for the assessment of vitamin D status
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, 920 1192, Japan
    Anal Bioanal Chem 391:229-38. 2008
  3. ncbi request reprint Characterization of in vitro metabolites of toad venom using high-performance liquid chromatography and liquid chromatography-mass spectrometry
    Kazutake Shimada
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biomed Chromatogr 20:1321-7. 2006
  4. doi request reprint Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS/MS: Method development and evaluation of applicability for diagnosis and medication for late-onset hypogonadism
    Yujin Shibayama
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2615-23. 2009
  5. ncbi request reprint Studies on neurosteroids XXV. Influence of a 5alpha-reductase inhibitor, finasteride, on rat brain neurosteroid levels and metabolism
    Yoshiyuki Mukai
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, Japan
    Biol Pharm Bull 31:1646-50. 2008
  6. doi request reprint Studies on neurosteroids XXIV. Determination of neuroactive androgens, androsterone and 5alpha-androstane-3alpha,17beta-diol, in rat brain and serum using liquid chromatography-tandem mass spectrometry
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biomed Chromatogr 22:1434-41. 2008
  7. ncbi request reprint Studies on neurosteroids XX. Liquid chromatography-tandem mass spectrometric method for simultaneous determination of testosterone and 5alpha-dihydrotestosterone in rat brain and serum
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr Sci 46:653-8. 2008
  8. ncbi request reprint Studies on Neurosteroids XXI: an improved liquid chromatography-tandem mass spectrometric method for determination of 5alpha-androstane-3alpha,17beta-diol in rat brains
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, Japan
    Anal Sci 23:1015-9. 2007
  9. ncbi request reprint Studies on neurosteroids XXIII. Analysis of tetrahydrocorticosterone isomers in the brain of rats exposed to immobilization using LC-MS
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Steroids 72:865-74. 2007
  10. doi request reprint Simultaneous determination of 17alpha-hydroxypregnenolone and 17alpha-hydroxyprogesterone in dried blood spots from low birth weight infants using LC-MS/MS
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 48:177-82. 2008

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Development and application of electrospray-active derivatization reagents for hydroxysteroids
    Tadashi Nishio
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 44:786-95. 2007
    ..These derivatization procedures coupled with LC-ESI-MS/MS were successfully used for the determination of estrogens in the serum and prostatic 5alpha-dihydrotestosterone...
  2. ncbi request reprint Liquid chromatography-tandem mass spectrometric method for the determination of salivary 25-hydroxyvitamin D3: a noninvasive tool for the assessment of vitamin D status
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, 920 1192, Japan
    Anal Bioanal Chem 391:229-38. 2008
    ..The method also enabled the detection of the increase in the salivary 25(OH)D(3) level after the supplementation of vitamin D(3)...
  3. ncbi request reprint Characterization of in vitro metabolites of toad venom using high-performance liquid chromatography and liquid chromatography-mass spectrometry
    Kazutake Shimada
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biomed Chromatogr 20:1321-7. 2006
    ..The structures of these metabolites were confirmed using authentic samples regarding their high-performance liquid chromatographic behavior and/or liquid chromatography-mass spectrometry analysis...
  4. doi request reprint Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS/MS: Method development and evaluation of applicability for diagnosis and medication for late-onset hypogonadism
    Yujin Shibayama
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2615-23. 2009
    ..Thus, the developed method has satisfactory applicability in the diagnosis and medication for LOH...
  5. ncbi request reprint Studies on neurosteroids XXV. Influence of a 5alpha-reductase inhibitor, finasteride, on rat brain neurosteroid levels and metabolism
    Yoshiyuki Mukai
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, Japan
    Biol Pharm Bull 31:1646-50. 2008
    ..No change was found in the brain levels of 3alpha-DHP, another GABAergic neurosteroid, and DOC by the administration of FIN...
  6. doi request reprint Studies on neurosteroids XXIV. Determination of neuroactive androgens, androsterone and 5alpha-androstane-3alpha,17beta-diol, in rat brain and serum using liquid chromatography-tandem mass spectrometry
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biomed Chromatogr 22:1434-41. 2008
    ....
  7. ncbi request reprint Studies on neurosteroids XX. Liquid chromatography-tandem mass spectrometric method for simultaneous determination of testosterone and 5alpha-dihydrotestosterone in rat brain and serum
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr Sci 46:653-8. 2008
    ....
  8. ncbi request reprint Studies on Neurosteroids XXI: an improved liquid chromatography-tandem mass spectrometric method for determination of 5alpha-androstane-3alpha,17beta-diol in rat brains
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, Japan
    Anal Sci 23:1015-9. 2007
    ..1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3alpha,5alpha-Adiol level by immobilization stress was also analyzed using the developed method...
  9. ncbi request reprint Studies on neurosteroids XXIII. Analysis of tetrahydrocorticosterone isomers in the brain of rats exposed to immobilization using LC-MS
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Steroids 72:865-74. 2007
    ..05), although the brain corticosterone level was not higher in the stressed female rats than in the male rats (no statistical difference)...
  10. doi request reprint Simultaneous determination of 17alpha-hydroxypregnenolone and 17alpha-hydroxyprogesterone in dried blood spots from low birth weight infants using LC-MS/MS
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 48:177-82. 2008
    ....
  11. ncbi request reprint Studies on neurosteroids XXII. Liquid chromatography-tandem mass spectrometric method for profiling rat brain 3-oxo-4-ene-neuroactive steroids
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biomed Chromatogr 22:34-43. 2008
    ..The limits of quantitation were 0.1 ng/g tissue for all the steroids. The application of this developed method for the analysis of changes in the brain neuroactive steroid levels by immobilization stress is also presented...
  12. ncbi request reprint Determination of salivary 17-ketosteroid sulfates using liquid chromatography-electrospray ionization-mass spectrometry
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 43:1782-8. 2007
    ..No significant matrix effect or change in the measured value by freeze/thaw repetitions was observed. The developed method was applied to clinical studies, and produced satisfactory results...
  13. doi request reprint Liquid chromatography-tandem mass spectrometric method for determination of salivary 17alpha-hydroxyprogesterone: a noninvasive tool for evaluating efficacy of hormone replacement therapy in congenital adrenal hyperplasia
    Yujin Shibayama
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 867:49-56. 2008
    ..The result from the salivary 17OHP measurement in patients with congenital adrenal hyperplasia demonstrated that the proposed method is very useful for monitoring of the therapeutic efficacy during hormone replacement therapy...
  14. ncbi request reprint Studies on neurosteroids XIX. Development and validation of liquid chromatography-tandem mass spectrometric method for determination of 5alpha-reduced pregnane-type neurosteroids in rat brain and serum
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 848:188-99. 2007
    ..0% for all the steroids in both the brain and serum. The application of the developed method to the analysis of changes in the brain and serum neurosteroid levels by immobilization stress and ethanol administration is also presented...
  15. ncbi request reprint Characterization of in vivo metabolites of toad venom using liquid chromatography-mass spectrometry
    Yoshimichi Miyashiro
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr Sci 46:534-8. 2008
    ..Bufalin is metabolized to 3-epibufalin, which is found to undergo further conjugation resulting in its 3-glucuronide. Furthermore, 3-epibufalin 3-sulfate is formed only in the female rats...
  16. ncbi request reprint Liquid chromatography-mass spectrometric assay of androstenediol in prostatic tissue: influence of androgen deprivation therapy on its level
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Steroids 71:1007-13. 2006
    ..83 +/- 0.28, 0.62 +/- 0.31 and 0.71 +/- 0.28 ng g(-1)tissue, respectively, and there was no significant difference between these groups...
  17. ncbi request reprint Studies on neurosteroids XVIII LC-MS analysis of changes in rat brain and serum testosterone levels induced by immobilization stress and ethanol administration
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Steroids 71:609-17. 2006
    ....
  18. ncbi request reprint Derivatization of neutral steroids to enhance their detection characteristics in liquid chromatography-mass spectrometry
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, 920 0934 Kanazawa, Japan
    Anal Bioanal Chem 378:875-82. 2004
    ..The introduction of moieties with proton affinity or electron affinity enhances the analyte signals in positive and negative atmospheric pressure chemical ionization MS, respectively...
  19. ncbi request reprint Detection and characterization of 20-oxosteroids in rat brains using LC-electron capture APCI-MS after derivatization with 2-nitro-4-trifluoromethylphenylhydrazine
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    Analyst 128:130-3. 2003
    ..Pregnenolone, progesterone, 5alpha-dihydroprogesterone, allopregnanolone and epiallopregnanolone were clearly detected with a 50 mg sample of the brain tissue...
  20. ncbi request reprint Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, Takara machi, Japan
    Biol Pharm Bull 26:709-11. 2003
    ..37-1.29 ng/g tissue). These results demonstrate that, in rats, the brain PREGS may be derived from peripheral sources, and its actual levels are much lower than those previously measured by indirect methods...
  21. ncbi request reprint Application of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-dione to analysis of 25-hydroxyvitamin D3 in human plasma by liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    Anal Sci 19:941-3. 2003
    ..This derivatization-LC/ECAPCI-MS method was applied to a plasma 25-hydroxyvitamin D3 assay, and gave satisfactory results in sensitivity, specificity, measurable range and throughput of the analysis...
  22. ncbi request reprint Simultaneous determination of androstenediol 3-sulfate and dehydroepiandrosterone sulfate in human serum using isotope diluted liquid chromatography-electrospray ionization-mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 796:121-30. 2003
    ..2-245.3 mg/ml (Adiol-3S) and 0.175-5.16 microg/ml (DHEA-S), and those of patients with prostate cancer (n=19) were 15.3-182.7 ng/ml (Adiol-3S; four samples, not detectable) and 0.110-2.421 microg/ml (DHEA-S)...
  23. ncbi request reprint Studies on neurosteroids XV. Development of enzyme-linked immunosorbent assay for examining whether pregnenolone sulfate is a veritable neurosteroid
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    J Pharm Biomed Anal 30:1907-17. 2003
    ..These results practically agreed with our previous results by liquid chromatography (LC)-electrospray ionization (ESI)-MS without deconjugation...
  24. ncbi request reprint Degradation of estrogen conjugates using titanium dioxide as a photocatalyst
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    Anal Sci 20:3-4. 2004
    ..90%), which were conjugated at the 3-hydroxy group, remained after a 6.5 h irradiation. These results supported the hypothesis that the photodegradation of estrogens was initiated at the phenolic hydroxy group...
  25. ncbi request reprint Analysis of C-3 epimerization in (24R)-24,25-dihydroxyvitamin D3 catalyzed by hydroxysteroid dehydrogenase
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 36:429-36. 2004
    ..The present study suggests that HSDs may catalyze the C-3 epimerization of vitamin D compounds and modulate their concentrations and biological activities in animals and humans...
  26. ncbi request reprint Electron-capturing derivatization of neutral steroids for increasing sensitivity in liquid chromatography/negative atmospheric pressure chemical ionization-mass spectrometry
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    Anal Sci 18:1301-7. 2002
    ..The developed derivatization procedures were applied to analyses of small amounts of steroids in human plasma and gave satisfactory results...
  27. ncbi request reprint Studies on neurosteroids XVII. Analysis of stress-induced changes in neurosteroid levels in rat brains using liquid chromatography-electron capture atmospheric pressure chemical ionization-mass spectrometry
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Steroids 70:1-11. 2005
    ..Thus, the present studies suggest that changes in the brain levels of neurosteroids may play an important role in the homeostatic mechanisms that counteract the inhibitory effect of stress on the GABA(A) receptor function...
  28. ncbi request reprint Enzymic conversion of 3beta-hydroxy-5-ene-steroids and their sulfates to 3-oxo-4-ene-steroids for increasing sensitivity in LC-APCI-MS
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Pharm Biomed Anal 39:718-23. 2005
    ..The method enabled the detection of trace levels of dehydroepiandrosterone and androstenediol 3-sulfate in human serum, which could not be detected by the usual LC-MS...
  29. ncbi request reprint Determination of prostatic androgens in 10 mg of tissue using liquid chromatography-tandem mass spectrometry with charged derivatization
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Anal Bioanal Chem 382:1035-43. 2005
    ..0 ng g(-1) tissue. The developed method was used to determine DHT and T in the prostates of patients with benign prostatic hyperplasia and prostate cancer, and satisfactory results were obtained...
  30. ncbi request reprint 2-hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 825:214-22. 2005
    ..The developed derivatization procedure was applied to the LC-ESI-MS analysis of 5alpha-dihydrotestosterone in human prostate, and allowed the reproducible quantification of nanogram/gram level of the androgen with a 10-mg sample...
  31. ncbi request reprint Determination of sulfates of androsterone and epiandrosterone in human serum using isotope diluted liquid chromatography-electrospray ionization-mass spectrometry
    Kuniko Mitamura
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biomed Chromatogr 19:796-801. 2005
    ..The levels of these androgen sulfates tended to decrease during aging, and the A-S levels in the sera obtained from both healthy males and patients with PC were correlated with their EpiA-S levels...
  32. ncbi request reprint Alternative procedure for charged derivatization to enhance detection responses of steroids in electrospray ionization-MS
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Japan
    Chem Pharm Bull (Tokyo) 55:662-5. 2007
    ..The derivatives also provided intense characteristic product ions in the MS-MS, which are expected to be usable for the selected reaction monitoring mode...
  33. ncbi request reprint Determination of salivary dehydroepiandrosterone using liquid chromatography--tandem mass spectrometry combined with charged derivatization
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 846:195-201. 2007
    ..No significant matrix effect or change in the measured value by freeze/thaw repetition was observed. The developed method was applied to clinical studies, and produced satisfactory results...
  34. ncbi request reprint Determination of digoxin in human serum using stable isotope dilution liquid chromatography/electrospray ionization-tandem mass spectrometry
    Kuniko Mitamura
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Biol Pharm Bull 30:1653-6. 2007
    ..20-3.20 ng/ml. The concentrations of digoxin in the serum samples obtained from digitalized patients (n=19) were in the range of 0.25-2.84 ng/ml, which were compared to those obtained by radioimmunoassay...
  35. ncbi request reprint Identification of dehydroepiandrosterone metabolites formed from human prostate homogenate using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry
    Kuniko Mitamura
    Faculty of Pharmaceutical Sciences, Kanazawa University, Japan
    J Chromatogr A 961:97-105. 2002
    ....
  36. ncbi request reprint Liquid chromatography--mass spectrometric method combined with derivatization for determination of 1 alpha-hydroxyvitamin D(3) in human plasma
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 772:229-38. 2002
    ..The limit of quantitation was 25 pg/ml for a 1.0-ml plasma aliquot. The application of the developed method to the sample of a volunteer orally given 1 alpha(OH)D(3) was also described...
  37. ncbi request reprint Procedure for increasing the detection responses of estrogens in LC-MS based on introduction of a nitrobenzene moiety followed by electron capture atmospheric pressure chemical ionization
    Tatsuya Higashi
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa, 920 1192, Japan
    Anal Bioanal Chem 386:658-65. 2006
    ..Tatsuya Higashiis Associate Professor of the Laboratory of Clinical Analytical Sciences (Professor Kazutake Shimada's research group) at the Graduate School of Natural Science and Technology of Kanazawa University...
  38. ncbi request reprint Determination method for steroid 5alpha-reductase activity using liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry
    Kuniko Mitamura
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    Anal Sci 21:1241-4. 2005
    ..The sum of the formed products was used as a measurement of the enzyme activity. This method was applied to kinetic study of this enzyme and inhibitory experiments using Finasteride as a model inhibitor...
  39. ncbi request reprint Characterization of urinary metabolites of vitamin D(3) in man under physiological conditions using liquid chromatography-tandem mass spectrometry
    Tatsuya Higashi
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, 920 0934, Kanazawa, Japan
    J Pharm Biomed Anal 29:947-55. 2002
    ..In addition, 23S,25-dihydroxy-24-oxovitamin D(3) occurred as its glucuronide in most of the urine, which suggested that this metabolite also plays a part in the excretion of vitamin D(3) in man...