M Sato

Summary

Affiliation: Kagoshima University
Country: Japan

Publications

  1. pmc The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890 8544, Japan
    Int J Mol Sci 17:. 2016
  2. pmc Targeted toxin-based selectable drug-free enrichment of Mammalian cells with high transgene expression
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890 8544, Japan
    Biology (Basel) 2:341-55. 2013
  3. pmc Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, 8 35 1 Sakuragaoka, Kagoshima, Kagoshima, 890 8544, Japan
    Biol Direct 11:16. 2016
  4. pmc Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890 8544, Japan
    Int J Mol Sci 16:17838-56. 2015
  5. doi request reprint A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Biotechnol J 10:143-53. 2015
  6. pmc A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
    Takayuki Sakurai
    Department of Cardiovascular Research, Graduate School of Medicine, Shinshu University, 3 1 1 Asahi, Matsumoto, Nagano 390 8621, Japan
    BMC Biotechnol 14:69. 2014
  7. pmc Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Biotechnol J 8:1355-61. 2013
  8. doi request reprint Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts
    M Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Reprod Domest Anim 47:759-65. 2012
  9. doi request reprint In vivo gene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and subsequent in vivo electroporation
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Syst Biol Reprod Med 58:278-87. 2012
  10. doi request reprint Development of a technique for efficient gene transfer to antral follicular cells in the mouse ovary
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Syst Biol Reprod Med 58:136-41. 2012

Collaborators

Detail Information

Publications47

  1. pmc The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890 8544, Japan
    Int J Mol Sci 17:. 2016
    ..These results indicate the feasibility of this PB-mediated method for simultaneous transfer of multigene constructs into the porcine cell genome, which is useful for production of cloned transgenic pigs expressing multiple transgenes. ..
  2. pmc Targeted toxin-based selectable drug-free enrichment of Mammalian cells with high transgene expression
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890 8544, Japan
    Biology (Basel) 2:341-55. 2013
    ..This system would be particularly valuable for researchers who wish to perform large-scale production of therapeutically important recombinant proteins. ..
  3. pmc Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, 8 35 1 Sakuragaoka, Kagoshima, Kagoshima, 890 8544, Japan
    Biol Direct 11:16. 2016
    ..In addition, several of the methods are re-evaluated with respect to their suitability to deliver genome editing components, particularly CRISPR/Cas9, to embryos...
  4. pmc Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890 8544, Japan
    Int J Mol Sci 16:17838-56. 2015
    ..Furthermore, it is suggested that zygote microinjection of mRNAs might lead to the production of piglets with cells harboring various mutation types. ..
  5. doi request reprint A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Biotechnol J 10:143-53. 2015
    ..These results indicated that the proposed approach is useful and efficient for obtaining stable transfectants without the use of drug-resistance genes, and offers a novel route for gene manipulation in cultured nonhuman mammalian cells. ..
  6. pmc A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
    Takayuki Sakurai
    Department of Cardiovascular Research, Graduate School of Medicine, Shinshu University, 3 1 1 Asahi, Matsumoto, Nagano 390 8621, Japan
    BMC Biotechnol 14:69. 2014
    ..However, it is not known whether indel mutations caused by the CRISPR/Cas9 system can be reproducibly detected in embryos. In this study, we assessed the detection of CRISPR/Cas9-induced mutations in embryos...
  7. pmc Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Biotechnol J 8:1355-61. 2013
    ..This novel gene delivery method to the pancreatic parenchyma may find application in gene therapy strategies for pancreatic diseases and in investigation of specific gene function in situ. ..
  8. doi request reprint Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts
    M Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Reprod Domest Anim 47:759-65. 2012
    ..Furthermore, these markers were found to be independent. The present information will be useful for the production of SCNT-mediated GM piglets that express multiple transgenes...
  9. doi request reprint In vivo gene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and subsequent in vivo electroporation
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Syst Biol Reprod Med 58:278-87. 2012
    ..This technology may provide a new method for manipulating preimplantation embryos in vivo, by using, for example, Cre-mediated conditional DNA recombination...
  10. doi request reprint Development of a technique for efficient gene transfer to antral follicular cells in the mouse ovary
    Masahiro Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Syst Biol Reprod Med 58:136-41. 2012
    ..This approach might be useful to explore the roles of genes related to oogenesis/folliculogenesis, and for reproductive manipulation targeted to antral follicles...
  11. pmc Development of CRTEIL and CETRIZ, Cre-loxP-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector
    Masato Ohtsuka
    Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    J Biomed Biotechnol 2009:985140. 2009
    ..These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion...
  12. doi request reprint Cultivation with untransfected fibroblasts stimulates proliferation of a single gene-modified fibroblast derived from a clawn miniature swine foetus
    M Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Reprod Domest Anim 46:911-6. 2011
    ..Thus, this method should be useful for the purification of target recombinant pig clones from mosaic populations that cannot be cultured as a single cell or for which suitable cell growth-promoting conditions are unclear...
  13. ncbi request reprint Usefulness of double gene construct for rapid identification of transgenic mice exhibiting tissue-specific gene expression
    M Sato
    Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    Mol Reprod Dev 60:446-56. 2001
    ....
  14. ncbi request reprint Differential expression of multidrug resistance (mdr) and canalicular multispecific organic anion transporter (cMOAT) genes following extrahepatic biliary obstruction in rats
    T Kagawa
    Department of Internal Medicine III, School of Medicine, Tokai University, Isehara, Kanagawa, Japan
    Biochem Mol Biol Int 44:443-52. 1998
    ..The expression of canalicular transporters was differentially regulated in extrahepatic biliary obstruction, indicating the different roles are played by mdr and cMOAT in the pathogenesis of cholestasis...
  15. ncbi request reprint Functional analysis after auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Japan
    Tohoku J Exp Med 189:295-305. 1999
    ..From this functional analysis, transplanted autologous IPE may have, in part, an alternative function in regard to the retinal pigment epithelium in the subretinal space...
  16. ncbi request reprint Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice
    M Sato
    Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, 259 1193, Kanagawa, Japan
    Biomol Eng 17:83-5. 2001
    ..All the blastocysts obtained by mating with the combination of Tg/Tg male (homozygous for transgene) x +/+ female exhibited fluorescence...
  17. ncbi request reprint Characterization of iris pigment epithelial cell for auto cell transplantation
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Miyagi, Japan
    Cell Transplant 8:501-10. 1999
    ..In the present study, we established auto IPE cell culture using auto serum. The cultured IPE cell showed pigment epithelial cell properties until around five passages in both human and monkey...
  18. ncbi request reprint New approach to cell lineage analysis in mammals using the Cre-loxP system
    M Sato
    Department of Molecular and Developmental Science, Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, Kanagawa, Japan
    Mol Reprod Dev 56:34-44. 2000
    ..Thus, our system, which is based on transient expression of the Cre recombinase gene directly introduced into nuclei of embryonic cells by microinjection, is a powerful means for cell lineage analysis in mammals...
  19. ncbi request reprint Possible mechanism of gene transfer into early to mid-gestational mouse fetuses by tail vein injection
    N Kikuchi
    Division of Molecular Life Science, School of Medicine, Tokai University, Isehara, Japan
    Gene Ther 9:1529-41. 2002
    ..If significantly improved, gene transfer to fetuses by injection into the maternal circulation may become a promising tool in fetal gene therapy and embryological studies...
  20. ncbi request reprint Molecular cloning of a leucine zipper motif-containing novel cDNA specifically expressed in adult mouse testis
    L Chen
    Department of Molecular Life Science, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa, Japan
    DNA Seq 9:101-8. 1998
    ..We therefore termed this novel cDNA tsec-2, testis-specifically expressed cDNAs-2. Results of Southern blot analysis suggested that the tsec-2 gene may exist as a single copy in the mouse genome...
  21. ncbi request reprint Sez4 gene encoding an elongation subunit of DNA polymerase zeta is required for normal embryogenesis
    K Kajiwara
    Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa 259 1193, Japan
    Genes Cells 6:99-106. 2001
    ..Although yeast REV3 homologues in mouse and human have recently been identified and characterized, their precise roles remain elusive...
  22. ncbi request reprint Intrabursal transfer of spermatozoa (ITS): a new route for artificial insemination of mice
    M Sato
    Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Isehara, Kanagawa, Japan
    Theriogenology 55:1881-90. 2001
    ....
  23. ncbi request reprint Abdominal lymphoma: differentiation from pancreatic carcinoma with Doppler US
    H Ishida
    First Department of Internal Medicine, Akita University, School of Medicine, 1 1 1 Hondo, Akita 010, Japan
    Abdom Imaging 27:461-4. 2002
    ..We investigated whether US and color Doppler US allowed differentiation between those pathologies...
  24. doi request reprint Generation of α-1,3-Galactosyltransferase-Deficient Porcine Embryonic Fibroblasts by CRISPR/Cas9-Mediated Knock-in of a Small Mutated Sequence and a Targeted Toxin-Based Selection System
    M Sato
    Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
    Reprod Domest Anim 50:872-80. 2015
    ....
  25. ncbi request reprint Prolonged survival of mouse epididymal spermatozoa stored at room temperature
    M Sato
    The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, Kanagawa, Japan
    Genesis 31:147-55. 2001
    ..3%) than that of embryos derived from in vitro fertilization with fresh spermatozoa. These findings indicate that spermatozoa kept for up to 3 days at 22 degrees C can fertilize oocytes, although at relatively low efficiency...
  26. doi request reprint Bovine Arboviruses in Culicoides Biting Midges and Sentinel Cattle in Southern Japan from 2003 to 2013
    T Kato
    Kyushu Research Station, National Institute of Animal Health, NARO, Kagoshima, Japan
    Transbound Emerg Dis 63:e160-e172. 2016
    ..Silent circulations of the other arboviruses, including potentially new viruses, were also detected during the study period...
  27. ncbi request reprint Overexpression of a minor component of myelin basic protein isoform (17.2 kDa) can restore myelinogenesis in transgenic shiverer mice
    M Kimura
    Division of Molecular Life Science, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259 11, Japan
    Brain Res 785:245-52. 1998
    ..2-kDa MBP isoform is present, has the ability to elicit CNS myelination in transgenic shi mice. This transgenic strategy will be useful for elucidating the role of each type of MBP isoform in CNS myelinogenesis...
  28. ncbi request reprint Gene structure and promoter for Crad2 encoding mouse cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase isozyme
    K Tomita
    Department of Molecular Life Science, School of Medicine, Tokai University, Bohseidai, Isehara, 259 1193, Kanagawa, Japan
    Gene 251:175-86. 2000
    ....
  29. ncbi request reprint Apoptosis in the lymphatic organs of piglets inoculated with classical swine fever virus
    M Sato
    Kyushu Research Station, National Institute of Animal Health, 2702 Chuzan, Kagoshima 891 0105, Kagoshima, Japan
    Vet Microbiol 75:1-9. 2000
    ..e. margination of condensed nuclear chromatin, were observed in the lymphatic organs from 2 DPI onward. These results suggest that apoptosis is involved in the pathology of CSF...
  30. ncbi request reprint Cloning and characterization of 5'-upstream sequence of the M32 gene for a mouse homologue of Drosophila heterochromatin protein 1 (HP1)
    M Sato
    Molecular Medicine Research Center, Institute of Medical Sciences, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    DNA Seq 12:97-106. 2001
    ..These findings suggest that the 5'-upstream region of the M32 gene consists of a multiple regulatory complex which probably plays important roles in nuclear function such as chromatin organization and RNA processing...
  31. ncbi request reprint HOXD3 regulates expression of JAGGED1, a ligand for Notch receptors
    Y Taniguchi
    Department of Genetic Information, School of Medicine, Tokai University, Isehara, Kanagawa 259 1193, Japan
    Nucleic Acids Res Suppl . 2001
    ..Consequently, HOXD3 promoted expression of JAGGED1, a ligand for Notch receptors, in both the transfectants, suggesting that the JAGGED1 gene is a downstream target of HOXD3...
  32. ncbi request reprint Cultured articular chondrocytes sheets for partial thickness cartilage defects utilizing temperature-responsive culture dishes
    N Kaneshiro
    Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Shimokasuya, Isehara, Kanagawa, Japan
    Eur Cell Mater 13:87-92. 2007
    ....
  33. pmc CD133 expression is correlated with lymph node metastasis and vascular endothelial growth factor-C expression in pancreatic cancer
    S Maeda
    Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Science, 8 35 1 Sakuragaoka, Kagoshima 890 8520, Japan
    Br J Cancer 98:1389-97. 2008
    ..0103). These results suggest that CD133 expression in pancreatic cancer was significantly associated with lymphatic metastasis, VEGF-C expression, and prognosis...
  34. doi request reprint Water nanodroplets make a greater contribution to facial skin moisture levels in air-conditioned rooms during winter than in summer
    H Ohno
    The Department of Physiology, School of Medicine, Aichi Medical University, Nagakute, Japan
    Skin Res Technol 21:207-13. 2015
    ..In the present study, we investigated the ability of mist to improve facial skin moisture levels in winter and summer...
  35. ncbi request reprint [Resection of malignant fibrous histiocytoma through a combined thoracic and abdominal wall approach]
    T Otsuka
    Department of Thoracic Surgery, Kagoshima University, Japan
    Kyobu Geka 65:389-92. 2012
    ..She received adjuvant radiotherapy as the tumor cells were detected in the surgical margin of the diaphragm. The patient has remained well without signs of recurrence for 10 months after the operation...
  36. ncbi request reprint Molecular cloning and analysis of novel cDNAs specifically expressed in adult mouse testes
    L Chen
    Department of Molecular Life Science, School of Medicine, Tokai University, Kanagawa, Japan
    Biochem Biophys Res Commun 240:261-8. 1997
    ..We therefore termed these novel clones tsec-1, testis-specifically expressed cDNAs-1. The protein products of tsec-1 may play an important role in mammalian spermatogenesis...
  37. ncbi request reprint Reduced formation of granulomata in gamma(delta) T cell knockout BALB/c mice inoculated with Mycobacterium avium subsp. paratuberculosis
    S Tanaka
    Kyushu Research Station, National Institute of Animal Health, Chuzan, Kagoshima, Japan
    Vet Pathol 37:415-21. 2000
    ..The results of this study may have relevance to our understanding of the pathogenesis of paratuberculosis in ruminants, in which a prominent number of gamma(delta) T cells exist in the lymphoid system...
  38. ncbi request reprint The effects of the elimination of Kupffer cells in the isolated perfused liver from non-heart-beating rat
    K Oikawa
    Second Department of Surgery, Tohoku University, School of Medicine, Sendai, Japan
    Transpl Int 13:S573-9. 2000
    ..These results indicated that the elimination of Kupffer cells improved sinusoidal microcirculation in NHBD and liver transplantation using grafts from NHBD could be made to succeed by modulation of Kupffer cells...
  39. ncbi request reprint Dexamethasone prevents acute cadmium-induced hepatic injury but exacerbates kidney dysfunction in rabbits
    H Horiguchi
    Department of Public Health, School of Medicine, Fukushima Medical University, 1 Hikarigoaka, Fukushima, 960 1295, Japan
    Toxicol Appl Pharmacol 174:225-34. 2001
    ..These results indicate that dexamethasone pretreatment potently prevented cadmium-induced liver injury, but exacerbated renal tubular dysfunction...
  40. doi request reprint Gender differences in the association between maternal smoking during pregnancy and childhood growth trajectories: multilevel analysis
    K Suzuki
    Department of Health Sciences, School of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan
    Int J Obes (Lond) 35:53-9. 2011
    ..This study examines the gender differences in the association between maternal smoking during pregnancy and later growth in childhood...
  41. ncbi request reprint Growth hormone prevents Fas-induced apoptosis in lymphocytes through modulation of Bcl-2 and caspase-3
    H Mitsunaka
    First Department of Internal Medicine, School of Medicine, Kagawa Medical University, Kita gun, Kagawa, Japan
    Neuroimmunomodulation 9:256-62. 2001
    ..However, the detailed mechanism of the effect of GH on the immune system has not yet been clarified. This study was designed to investigate the nature of this mechanism...
  42. ncbi request reprint Association of nonpolio enteroviral infection in the central nervous system of children with febrile seizures
    M Hosoya
    Department of Pediatrics, School of Medicine, Fukushima Medical University, Fukushima, Japan
    Pediatrics 107:E12. 2001
    ..To clarify the relationship between enteroviral infection and febrile seizures...
  43. ncbi request reprint Indomethacin induced bulky lymphadenopathy and eosinophilic pneumonia
    Y Oishi
    Second Department of Internal Medicine, Gunma University, School of Medicine, University of Tokyo, Tokyo, Japan
    Respirology 6:57-60. 2001
    ..An allergic reaction to NSAID should be considered as a rare cause of mediastinal lymphadenopathy...
  44. ncbi request reprint Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration: short-term results
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Japan
    Tohoku J Exp Med 191:7-20. 2000
    ..In conclusion, the treatment resulted in no significant improvement in macular function, as compared with the control; however, no rejection or deterioration in visual acuity occurred up to the 13 month follow up...
  45. ncbi request reprint Mesenteric lipoma: report of a case with emphasis on US findings
    M Sato
    School of Medicine, Akita University, 1 1 1 Hondo, Akita, Japan
    Eur Radiol 12:793-5. 2002
    ..Color Doppler US and angiography confirmed the avascular nature of the mass. Knowledge of the US findings in this case is useful for the diagnosis of ML...
  46. ncbi request reprint Phrenic nerve conduction in infancy and early childhood
    T Imai
    Department of Neurology, School of Medicine, Sapporo Medical University, South 1, West 16, Chuo Ku, Sapporo 060 8543, Japan
    Muscle Nerve 23:915-8. 2000
    ..The results are valuable not only as a reference for the diagnosis of patients with phrenic nerve palsy, but also as an indicator of the normal development of the phrenic nerve...
  47. ncbi request reprint Absence of R1066X mutation in six Japanese patients with Dubin-Johnson syndrome
    T Kagawa
    Department of Internal Medicine, School of Medicine, Tokai University, Isehara, Japan
    Biochem Mol Biol Int 47:639-44. 1999
    ..These findings suggested that this R1066X mutation was not a major mutation in Japanese patients with DJS. Further investigation will be required in an attempt to search other mutations in cMOAT gene in Japanese patients with DJS...