Chiharu Nishiyama

Summary

Affiliation: Juntendo University School of Medicine
Country: Japan

Publications

  1. ncbi request reprint Splice isoforms of transcription factor Elf-1 affecting its regulatory function in transcription-molecular cloning of rat Elf-1
    C Nishiyama
    Foods and Pharmaceuticals Research and Development Laboratory, Asahi Breweries, Ltd, Kitasoma gun, Ibaraki, Japan
    Biosci Biotechnol Biochem 64:2601-7. 2000
  2. ncbi request reprint Molecular mechanism of allergy-related gene regulation and hematopoietic cell development by transcription factors
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, Japan
    Biosci Biotechnol Biochem 70:1-9. 2006
  3. ncbi request reprint GATA-1 is required for expression of Fc{epsilon}RI on mast cells: analysis of mast cells derived from GATA-1 knockdown mouse bone marrow
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Int Immunol 17:847-56. 2005
  4. ncbi request reprint Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy
    Chiharu Nishiyama
    Atopy Allergy Research Center, Department of Dermatology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Immunol 173:6458-64. 2004
  5. ncbi request reprint Evidence against requirement of Ser41 and Ser45 for function of PU.1 -- molecular cloning of rat PU.1
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    FEBS Lett 572:57-64. 2004
  6. ncbi request reprint Functional analysis of PU.1 domains in monocyte-specific gene regulation
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    FEBS Lett 561:63-8. 2004
  7. ncbi request reprint Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 313:516-21. 2004
  8. ncbi request reprint Molecular cloning of rat transcription factor YY1
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Biosci Biotechnol Biochem 67:654-8. 2003
  9. ncbi request reprint Cloning of full-length genomic DNA encoding human FcepsilonRI alpha-chain and its transcriptional regulation
    C Nishiyama
    Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Biochem Biophys Res Commun 284:1056-64. 2001
  10. ncbi request reprint Regulation of human Fc epsilon RI alpha-chain gene expression by multiple transcription factors
    Chiharu Nishiyama
    Allergy Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 168:4546-52. 2002

Collaborators

Detail Information

Publications65

  1. ncbi request reprint Splice isoforms of transcription factor Elf-1 affecting its regulatory function in transcription-molecular cloning of rat Elf-1
    C Nishiyama
    Foods and Pharmaceuticals Research and Development Laboratory, Asahi Breweries, Ltd, Kitasoma gun, Ibaraki, Japan
    Biosci Biotechnol Biochem 64:2601-7. 2000
    ..This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control...
  2. ncbi request reprint Molecular mechanism of allergy-related gene regulation and hematopoietic cell development by transcription factors
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, Japan
    Biosci Biotechnol Biochem 70:1-9. 2006
    ..Another interesting finding on transcription factors functioning in mast cells is that the expression level of PU.1 determines cell fate between mast cells and monocytes...
  3. ncbi request reprint GATA-1 is required for expression of Fc{epsilon}RI on mast cells: analysis of mast cells derived from GATA-1 knockdown mouse bone marrow
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Int Immunol 17:847-56. 2005
    ..We concluded that the transcription factor GATA-1 positively regulates FcepsilonRI alpha- and beta-chain expression and therefore is involved in mast cell development...
  4. ncbi request reprint Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy
    Chiharu Nishiyama
    Atopy Allergy Research Center, Department of Dermatology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Immunol 173:6458-64. 2004
    ..These results suggest that the SNPs in the FcepsilonRIbeta promoter are causally linked with atopy via regulation of FcepsilonRI expression...
  5. ncbi request reprint Evidence against requirement of Ser41 and Ser45 for function of PU.1 -- molecular cloning of rat PU.1
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    FEBS Lett 572:57-64. 2004
    ..From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter-upregulating function of PU.1...
  6. ncbi request reprint Functional analysis of PU.1 domains in monocyte-specific gene regulation
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    FEBS Lett 561:63-8. 2004
    ..1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression...
  7. ncbi request reprint Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 313:516-21. 2004
    ..1-overproducing cells exhibited the morphology, in part, similar to that of monocyte. These results indicate that the mast cell progenitors still have the ability to express monocyte-specific genes by increased expression of PU.1...
  8. ncbi request reprint Molecular cloning of rat transcription factor YY1
    Chiharu Nishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Biosci Biotechnol Biochem 67:654-8. 2003
    ..On the other hand, N-terminus of the activation region (1-78/100-106) did not have transactivation activity by itself as well as synergistic activity with an erythroid specific transcription factor GATA-1...
  9. ncbi request reprint Cloning of full-length genomic DNA encoding human FcepsilonRI alpha-chain and its transcriptional regulation
    C Nishiyama
    Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Biochem Biophys Res Commun 284:1056-64. 2001
    ..Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in FcepsilonRI alpha-chain positive cells...
  10. ncbi request reprint Regulation of human Fc epsilon RI alpha-chain gene expression by multiple transcription factors
    Chiharu Nishiyama
    Allergy Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 168:4546-52. 2002
    ..Transcriptional regulation of the alpha-chain gene through four transcriptional factors is discussed...
  11. ncbi request reprint Analysis of transactivation region of Elf-1 by using a yeast one-hybrid system
    Chiharu Nishiyama
    Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    Biosci Biotechnol Biochem 66:1105-7. 2002
    ..The transcriptional regulatory region of Elf-1 was analyzed by the combination of a yeast one-hybrid system and site-directed mutagenesis. This analysis enabled us to map an activation region between 85-175 of Elf-1...
  12. ncbi request reprint A novel -66T/C polymorphism in Fc epsilon RI alpha-chain promoter affecting the transcription activity: possible relationship to allergic diseases
    Masanari Hasegawa
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 171:1927-33. 2003
    ..Our findings for the first time demonstrate the presence of FcepsilonRIalpha polymorphism related to the allergic diseases...
  13. doi request reprint Roles of PU.1 in monocyte- and mast cell-specific gene regulation: PU.1 transactivates CIITA pIV in cooperation with IFN-gamma
    Tomonobu Ito
    Atopy Allergy Research Center, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    Int Immunol 21:803-16. 2009
    ..1 transfectants indicate that enforced PU.1 suppresses mast cell-specific gene expression through these transcription factors...
  14. doi request reprint Suppressive effect of Elf-1 on FcepsilonRI alpha-chain expression in primary mast cells
    Qing Hui Wang
    Atopy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Immunogenetics 60:557-63. 2008
    ..These results indicate that Elf-1 negatively regulates FcepsilonRI alpha-chain expression by suppressing PU.1-mediated transcription of the alpha-chain in BMMC...
  15. ncbi request reprint Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants: molecular cloning of rat Icsbp (Irf-8) cDNA
    Nobuhiro Nakano
    Atopy Allergy Research Center, Juntendo University School of Medicine, Bunkyo ku, Tokyo 113 8421 Japan
    Immunogenetics 56:871-7. 2005
    ..1/ICSBP/DNA-complex formation. These results indicated that Ser147 of PU.1 is definitively required for forming a complex with ICSBP and phosphorylation of PU.1 and/or ICSBP is critical for formation of the ternary complex...
  16. doi request reprint Opposite effects of PU.1 on mast cell stimulation
    Yusuke Niwa
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 375:95-100. 2008
    ..These observations indicate that up-regulation of PU.1 suppresses expression of FcepsilonRI signal transduction-related intracellular molecules, but increases the potential of transcription activity of monocyte characters...
  17. pmc Notch1-mediated signaling induces MHC class II expression through activation of class II transactivator promoter III in mast cells
    Nobuhiro Nakano
    Atopy Allergy Research Center, Department of Immunology, Juntendo Univesity School of Medicine, Tokyo, Japan
    J Biol Chem 286:12042-8. 2011
    ..These findings indicate that activation of CIITA pIII plays an important role in MHC class II expression in mast cells...
  18. doi request reprint Suppressive effects of transcription factor GATA-1 on cell type-specific gene expression in dendritic cells
    Naomi Shimokawa
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Immunogenetics 62:421-9. 2010
    ..These results indicate that GATA-1 suppresses PU.1 function but not PU.1 transcription. Thus, GATA-1 appears to determine cell fate by regulating several cell-specific transcription factors...
  19. ncbi request reprint Two different transcription factors discriminate the -315C>T polymorphism of the Fc epsilon RI alpha gene: binding of Sp1 to -315C and of a high mobility group-related molecule to -315T
    Shunsuke Kanada
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 180:8204-10. 2008
    ..The findings demonstrate that a -315 SNP significantly affects human FcepsilonRI alpha-chain promoter activity and expression level of FcepsilonRI on basophils by binding different transcription factors to the SNP site...
  20. doi request reprint Involvement of PU.1 in the transcriptional regulation of TNF-alpha
    Tatsuo Fukai
    Atopy Research Center, Juntendo University School of Medicine, Hongo, Bunkyo ku, Tokyo, Japan
    Biochem Biophys Res Commun 388:102-6. 2009
    ..These observations indicate that PU.1 transactivates the TNF-alpha promoter and that the amount of PU.1 binding on the promoter is associated with promoter activity...
  21. doi request reprint Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation
    Nobuhiro Nakano
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    Int Arch Allergy Immunol 147:213-21. 2008
    ....
  22. ncbi request reprint Mast cells acquire monocyte-specific gene expression and monocyte-like morphology by overproduction of PU.1
    Tomonobu Ito
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Immunol 174:376-83. 2005
    ..However, surface level of CD11c and MHC class II was not affected. These results indicate that the potential capacity to exhibit monocytic features is different between both the mast cells...
  23. ncbi request reprint Regulation of the human Fc epsilon RI alpha-chain distal promoter
    Masanari Hasegawa
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 170:3732-8. 2003
    ..These results indicate that IL-4-dependent up-regulation of the human alpha-chain was due to enhancement of distal promoter activity and suggests that the two promoters have different regulatory mechanisms for alpha-chain expression...
  24. ncbi request reprint Role of Sp1 in transcription of human ATP2A2 gene in keratinocytes
    Atsushi Takagi
    Atopy Research Center, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    J Invest Dermatol 128:96-103. 2008
    ..This is early evidence demonstrating that Sp1 plays an important and positive role in ATP2A2 gene expression in NHK in vivo and in vitro...
  25. doi request reprint Role of PU.1 in MHC class II expression through transcriptional regulation of class II transactivator pI in dendritic cells
    Nao Kitamura
    Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Allergy Clin Immunol 129:814-824.e6. 2012
    ..PU.1 is a hematopoietic cell-specific transcription factor belonging to the Ets family. We hypothesized that PU.1 is involved in MHC class II expression in dendritic cells (DCs)...
  26. doi request reprint Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2
    Eisuke Inage
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, 113 8421, Japan
    J Immunol 192:3936-46. 2014
    ..Our findings will contribute to the development of new therapeutic approaches for FcεRI-mediated allergic diseases. ..
  27. ncbi request reprint Regulation of cell type-specific mouse Fc epsilon RI beta-chain gene expression by GATA-1 via four GATA motifs in the promoter
    Keiko Maeda
    Atopy Allergy Research Center, Juntendo University School of Medicine, University of Tokyo, Japan
    J Immunol 170:334-40. 2003
    ..These results indicate that cell type-specific transcription of mouse beta-chain gene is regulated by GATA-1...
  28. doi request reprint Transcriptional regulation of the mouse CD11c promoter by AP-1 complex with JunD and Fra2 in dendritic cells
    Mutsuko Hara
    Atopy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Mol Immunol 53:295-301. 2013
    ..These results indicate that AP-1 composed with JunD and Fra2 protein plays a primary role in enhancing the transcription level of the CD11c gene in DC...
  29. doi request reprint Interleukin-10 gene-transfected mature dendritic cells suppress murine experimental autoimmune optic neuritis
    Ryusaku Matsuda
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
    Invest Ophthalmol Vis Sci 53:7235-45. 2012
    ..In our study, we examined whether IL-10-transfected mDC prevent development of EAON and EAE...
  30. doi request reprint GATA2 and Sp1 positively regulate the c-kit promoter in mast cells
    Keiko Maeda
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Immunol 185:4252-60. 2010
    ..These findings indicate that GATA2 and Sp1 play crucial roles in expression of the c-kit gene in mast cells...
  31. ncbi request reprint Regulation of human FcepsilonRI beta chain gene expression by Oct-1
    Yushiro Akizawa
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Int Immunol 15:549-56. 2003
    ..Regulation of beta chain gene expression is discussed...
  32. doi request reprint Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus
    Takuya Ouchi
    Biotechnology Research Center, The University of Tokyo, Tokyo, Japan
    Nat Chem Biol 9:277-83. 2013
    ..Phylogenetic analysis reveals that gene duplication events at different stages of evolution led to ArgX and LysX...
  33. pmc GATA2 is a critical transactivator for the human IL1RL1/ST2 promoter in mast cells/basophils: opposing roles for GATA2 and GATA1 in human IL1RL1/ST2 gene expression
    Yosuke Baba
    Atopy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Biol Chem 287:32689-96. 2012
    ..These results indicate that GATA2 and GATA1 positively and negatively control human ST2 gene transcription, respectively...
  34. doi request reprint Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells
    Shunsuke Kanada
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    Blood 117:2211-22. 2011
    ..1 siRNA or topical application of a cream-emulsified PU.1 siRNA efficiently inhibited murine contact hypersensitivity. Our results suggest that PU.1 is a potential target for the treatment of immune-related diseases...
  35. ncbi request reprint Prolonged MHC class II expression and CIITA transcription in human keratinocytes
    Atsushi Takagi
    Department of Dermatology, Juntendo University School of Medicine, 2 1 1 Hongo, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 347:388-93. 2006
    ..This difference subsequently caused significantly higher expression at 72 h of MHC class II on NHK, compared with THP-1. This is the first report to quantitatively analyze each type of CIITA transcript in NHK...
  36. ncbi request reprint B-transferase with a Pro234Ser substitution acquires AB-transferase activity
    Chiyomi Nishida
    Atopy Allergy Research Center, Juntendo University School of Medicine, Hongo, Bunkyo ku, Tokyo, Japan
    Biosci Biotechnol Biochem 75:1570-5. 2011
    ..Molecular simulation indicated that the P234S mutation causes a conformational change in the substrate pocket making it suitable for N-acetylgalactosamine...
  37. doi request reprint Opposite effects of Trichostatin A on activation of mast cells by different stimulants
    Qing Hui Wang
    Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    FEBS Lett 584:2315-20. 2010
    ..TSA-treatment inhibited nuclear translocation of NF-kappaB following IgE-mediated, but not LPS-induced activation in MCs...
  38. ncbi request reprint FOG-1 represses GATA-1-dependent FcepsilonRI beta-chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice
    Keiko Maeda
    Atopy Allergy Research Center and Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan
    Blood 108:262-9. 2006
    ..These results suggest that FOG-1 expression level regulates the GATA-1-dependent FcepsilonRI beta-chain promoter...
  39. doi request reprint FcepsilonRIalpha gene -18483A>C polymorphism affects transcriptional activity through YY1 binding
    Daniel P Potaczek
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Immunogenetics 61:649-55. 2009
    ..Indeed, we confirmed preferential binding of the YY1 transcription factor to the -18483C allele, resulting in lower transcriptional activity when compared with the -18483A allele...
  40. ncbi request reprint Transcriptional regulation of ATP2C1 gene by Sp1 and YY1 and reduced function of its promoter in Hailey-Hailey disease keratinocytes
    Hiroshi Kawada
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Invest Dermatol 124:1206-14. 2005
    ..This is a report describing the regulation of the expression of ATP2C1...
  41. ncbi request reprint Inhibitory effect of polyphenol-enriched apple extracts on mast cell degranulation in vitro targeting the binding between IgE and FcepsilonRI
    Tomoko Tokura
    Atopy Allergy Research Center, Juntendo University School of Medicine, Hongo, Tokyo, Japan
    Biosci Biotechnol Biochem 69:1974-7. 2005
    ..This is the first report demonstrating the molecular mechanism for the anti-allergic effect of procyanidin-enriched extracts from apples...
  42. doi request reprint Notch signaling confers antigen-presenting cell functions on mast cells
    Nobuhiro Nakano
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    J Allergy Clin Immunol 123:74-81.e1. 2009
    ..Notch signaling is involved in cell fate determination along with the development of the immune system. However, very little is known about the role for Notch signaling in mast cells...
  43. doi request reprint Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus
    Akira Horie
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Nat Chem Biol 5:673-9. 2009
    ..This study thus reveals the previously unknown function of a small protein in primary metabolism...
  44. doi request reprint Involvement of PU.1 in mast cell/basophil-specific function of the human IL1RL1/ST2 promoter
    Yosuke Baba
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    Allergol Int 61:461-7. 2012
    ..In the present study, we analyzed regulation mechanism of the human ST2 promoter in the human mast cell line LAD2 and basophilic cell line KU812...
  45. ncbi request reprint Involvement of mast cells in IL-12/23 p40 production is essential for survival from polymicrobial infections
    Nobuhiro Nakano
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Blood 109:4846-55. 2007
    ..These results demonstrate that mast cells play a significant role in the production of IL-12 required for host defense. This is the first report to demonstrate that mast cells are a crucial source of functional IL-12...
  46. doi request reprint Genetic variability of the high-affinity IgE receptor alpha-subunit (FcepsilonRIalpha)
    Daniel P Potaczek
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Immunol Res 45:75-84. 2009
    ....
  47. pmc Role of PU.1 in MHC Class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells
    Ryosuke Miura
    Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6 3 1 Niijuku, Katsushika ku, Tokyo, 125 8585, Japan
    PLoS ONE 11:e0154094. 2016
    ..The GM-CSF-mediated up-regulation of these mRNAs was canceled in PU.1 siRNA-introduced cells. Taking these results together, we conclude that PU.1 transactivates the pIII through direct binding to Ets-motifs in the promoter in pDCs. ..
  48. doi request reprint Pharmacologic inhibition of Notch signaling suppresses food antigen-induced mucosal mast cell hyperplasia
    Asuka Honjo
    Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan
    J Allergy Clin Immunol . 2016
    ..Mucosal mast cells (MMCs) play a central role in the development of symptoms associated with IgE-mediated food allergy. Recently, Notch2-mediated signaling was shown to be involved in proper MMC distribution in the intestinal tract...
  49. doi request reprint A comparative search for human FcεRIα gene (FCER1A) 3'-UTR polymorphisms in Japanese and Polish populations
    Daniel P Potaczek
    Atopy Allergy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, 113 8421, Tokyo, Japan
    Mol Biol Rep 39:3747-53. 2012
    ....
  50. doi request reprint Establishment of a simple detection system for blood group ABO-specific transferase activity in DNA-transfected cells
    Chiyomi Nishida
    Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan
    Leg Med (Tokyo) 12:172-6. 2010
    ..Accordingly, the present study demonstrates a possibility that A/B-transferase variants may be characterized by using this method...
  51. ncbi request reprint Human umbilical cord epithelial cells express Notch1: implications for its epidermal-like differentiation
    William Ng
    Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan
    J Dermatol Sci 49:143-52. 2008
    ..As such, we hypothesized that Notch1 may be present in human umbilical cord epithelial cells (HUCEC)...
  52. doi request reprint Involvement of PU.1 in NFATc1 promoter function in osteoclast development
    Kentaro Ishiyama
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan
    Allergol Int 64:241-7. 2015
    ..However, the existence of cross-regulation between NFATc1 and PU.1 is largely unknown. In the present study, the role of PU.1 in NFATc1 expression was investigated...
  53. doi request reprint The Transcription Factor Ehf Is Involved in TGF-β-Induced Suppression of FcεRI and c-Kit Expression and FcεRI-Mediated Activation in Mast Cells
    Susumu Yamazaki
    Atopy Allergy Research Center, Juntendo University Graduate School of Medicine, Tokyo 113 8421, Japan Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo 113 8421, Japan and
    J Immunol 195:3427-35. 2015
    ..In part, mast cell response to TGF-β1 was mimicked by forced expression of Ehf, suggesting that TGF-β1 suppresses FcεRI and c-Kit expression and suppresses FcεRI-mediated activation through upregulation of Ehf. ..
  54. doi request reprint Suppression of murine experimental autoimmune optic neuritis by mature dendritic cells transfected with calcitonin gene-related Peptide gene
    Ryusaku Matsuda
    Department of Ophthalmology, Tokyo Medical University, Shinjuku ku, Tokyo, Japan
    Invest Ophthalmol Vis Sci 53:5475-85. 2012
    ..We examined whether CGRP-transfected dendritic cells (DC) prevent the development of experimental autoimmune optic neuritis (EAON) and experimental autoimmune encephalomyelitis (EAE)...
  55. pmc Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity
    Taisuke Sawada
    Department of Immunology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Biol Chem 283:26820-8. 2008
    ..Collectively, these functional and spatial changes of OTT and BSAC caused by the fusion might perturb their functions, culminating in the development of acute megakaryoblastic leukemia...
  56. pmc PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells
    Takuya Yashiro
    Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6 3 1 Niijuku, Katsushika ku, Tokyo, 125 8585, Japan Atopy Research Center, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    PLoS ONE 10:e0137699. 2015
    ..1 siRNA-transfected stimulated DCs. Our results indicate that PU.1 plays pivotal roles in DC development and function, serving not only as a transcriptional activator but also as a repressor. ..
  57. pmc Structural insight into amino group-carrier protein-mediated lysine biosynthesis: crystal structure of the LysZ·LysW complex from Thermus thermophilus
    Ayako Yoshida
    From the Biotechnology Research Center, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, 113 8657
    J Biol Chem 290:435-47. 2015
    ....
  58. doi request reprint Notch signaling enhances FcεRI-mediated cytokine production by mast cells through direct and indirect mechanisms
    Nobuhiro Nakano
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Immunol 194:4535-44. 2015
    ....
  59. pmc Critical Role of Transcription Factor PU.1 in the Function of the OX40L/TNFSF4 Promoter in Dendritic Cells
    Takuya Yashiro
    Laboratory of Molecular Biology and Immunology, Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6 3 1 Niijuku, Katsushika ku, Tokyo, 125 8585, Japan
    Sci Rep 6:34825. 2016
    ..In the present study, the structure of the OX40L promoter regulated by PU.1 is determined. It is also suggested that PU.1 is involved in mouse OX40L expression via multiple binding sites on the gene...
  60. pmc IL-33 promotes MHC class II expression in murine mast cells
    Tomonobu Ito
    Department of Dermatology, Tokyo Medical University 6 7 1 Nishi shinjuku, Shinjuku ku, Tokyo 160 0023, Japan
    Immun Inflamm Dis 3:196-208. 2015
    ..IL-33 is an important cytokine in allergic disorders. Mast cells have the ability to express MHC class II after prolonged exposure to IL-33 in a murine model. IL-33 holds a key to understanding the etiology of atopic dermatitis...
  61. doi request reprint C/EBPα controls mast cell function
    Kazumi Kasakura
    College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan Atopy Allergy Research Center, Juntendo University School of Medicine, Bunkyo, Tokyo, Japan Department of Biological Science and Technology, Tokyo University of Science, Katsushika, Tokyo, Japan
    FEBS Lett 588:4645-53. 2014
    ..This suggests that intestinal commensal bacteria modulate C/EBPα expression and thereby regulate mast cell function. ..
  62. ncbi request reprint Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus
    Taishi Tsubouchi
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 280:18511-6. 2005
    ..This is the first report demonstrating the transcriptional regulation of amino acid biosynthesis in T. thermophilus...
  63. doi request reprint Very rare minor homozygous GG genotype of tissue factor +5466A>G mutation in a patient with two cryptogenic cerebrovascular ischemic events
    Daniel P Potaczek
    Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan
    Int J Cardiol 147:e13-5. 2011
    ..There is a need to conduct a prospective study on the effect of TF +5466A>G polymorphism on the risk of cryptogenic stroke...
  64. ncbi request reprint Transcriptional regulation of the human high affinity IgE receptor alpha-chain gene
    Kyoko Takahashi
    Department of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University School of Medicine, 30 1 Oyaguchi Kamimachi, Itabashi ku, 173 8610, Tokyo, Japan
    Mol Immunol 38:1193-9. 2002
    ..Furthermore, we found two novel exons at 18.4 and 12.6kb upstream from the reported first exon and discovered an additional distal promoter...
  65. ncbi request reprint Regulation of the human high affinity IgE receptor beta-chain gene expression via an intronic element
    Kyoko Takahashi
    Division of Molecular Cell Immunology and Allergology, Nihon University Graduate School of Medical Sciences, 30 1 Oyaguchi, Kami machi, Itabashi ku, Tokyo 173 8610, Japan
    J Immunol 171:2478-84. 2003
    ..Furthermore, it was suggested that a cofactor binding with MZF-1, whose expression level was different among the cell types, was required for transcriptional repression by MZF-1...